MX2020006884A - Metodos de purificacion de virus adenoasociado. - Google Patents

Metodos de purificacion de virus adenoasociado.

Info

Publication number
MX2020006884A
MX2020006884A MX2020006884A MX2020006884A MX2020006884A MX 2020006884 A MX2020006884 A MX 2020006884A MX 2020006884 A MX2020006884 A MX 2020006884A MX 2020006884 A MX2020006884 A MX 2020006884A MX 2020006884 A MX2020006884 A MX 2020006884A
Authority
MX
Mexico
Prior art keywords
adeno
associated virus
purification methods
virus purification
aav
Prior art date
Application number
MX2020006884A
Other languages
English (en)
Inventor
Meinhard Hasslacher
Christian Fiedler
Jadranka Koehn
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of MX2020006884A publication Critical patent/MX2020006884A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Medicinal Preparation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Se proporcionan en la presente métodos para la producción de un producto de virus adenoasociado (AAV) y métodos para la purificación de virus adenoasociados. El AAV se carga en una resina de afinidad, se llevan a cabo etapas de lavado y el AAV se eluye de la resina de afinidad. Se describen diversos amortiguadores para usarlos en las etapas de lavado y elución.
MX2020006884A 2017-12-29 2018-12-27 Metodos de purificacion de virus adenoasociado. MX2020006884A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762611709P 2017-12-29 2017-12-29
PCT/US2018/067627 WO2019133677A1 (en) 2017-12-29 2018-12-27 Adeno-associated virus purification methods

Publications (1)

Publication Number Publication Date
MX2020006884A true MX2020006884A (es) 2020-10-01

Family

ID=67064154

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020006884A MX2020006884A (es) 2017-12-29 2018-12-27 Metodos de purificacion de virus adenoasociado.

Country Status (12)

Country Link
US (1) US11981934B2 (es)
EP (1) EP3731863A4 (es)
JP (2) JP7299896B2 (es)
KR (1) KR20200106045A (es)
CN (1) CN111655285A (es)
AU (1) AU2018395254A1 (es)
BR (1) BR112020013197A8 (es)
CA (1) CA3084827A1 (es)
IL (1) IL275533A (es)
MX (1) MX2020006884A (es)
SG (1) SG11202006232SA (es)
WO (1) WO2019133677A1 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020014479A1 (en) * 2018-07-11 2020-01-16 Baxalta Incorporated Aav compositions
CN114173827A (zh) * 2019-06-28 2022-03-11 武田药品工业株式会社 腺相关病毒纯化方法
EP3868886A1 (en) * 2020-02-21 2021-08-25 Bia Separations D.O.O. A method for separation or depletion of empty aav capsids from full aav capsids
US20220033782A1 (en) * 2020-07-29 2022-02-03 Pall Corporation Adenovirus-associated viruses separation method
MX2023005218A (es) * 2020-11-03 2023-05-16 Pfizer Metodos de purificacion de vectores de aav mediante cromatografia de intercambio anionico.
US20240043869A1 (en) * 2020-12-23 2024-02-08 Pfizer Inc. Methods for purification of aav vectors by affinity chromatography
JP2024503896A (ja) * 2021-01-21 2024-01-29 アスクレピオス バイオファーマシューティカル， インコーポレイテッド 組換えウイルス粒子を精製するための方法
CN114591921A (zh) * 2022-03-31 2022-06-07 上海勉亦生物科技有限公司 腺相关病毒的纯化方法和洗脱液
WO2022222869A1 (zh) * 2021-04-19 2022-10-27 信念医药（香港）有限公司 重组腺相关病毒及其应用
CN113552349B (zh) * 2021-07-30 2022-07-19 上海勉亦生物科技有限公司 Aav蛋白外壳的检测方法
WO2023077085A2 (en) * 2021-10-29 2023-05-04 Oxford Biomedica Solutions Llc Methods and compositions for the purification of adeno-associated virus
CN114350622A (zh) * 2022-01-17 2022-04-15 苏州博腾生物制药有限公司 一种病毒纯化高效保护试剂及其应用
TW202342522A (zh) 2022-03-07 2023-11-01 日商武田藥品工業股份有限公司 臨床人類ｉｇｇ產品之親和層析生產
GB202206123D0 (en) * 2022-04-27 2022-06-08 Cytiva Bioprocess R & D Ab A pre-screening method and a method for separating adeno-associated virus capsids

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4543363A (en) 1983-06-15 1985-09-24 Asahi Kasei Kogyo Kabushiki Kaisha Ion exchanger having hydroxyl groups bonded directly to backbone skeleton
SE9902133D0 (sv) 1999-06-06 1999-06-06 Amersham Pharm Biotech Ab A method for surface modification, a novel support matrix and the use of the matrix
GB9925432D0 (en) 1999-10-27 1999-12-29 Univ London Virus preparation
US6593123B1 (en) 2000-08-07 2003-07-15 Avigen, Inc. Large-scale recombinant adeno-associated virus (rAAV) production and purification
MX2011013613A (es) 2009-06-16 2012-01-19 Genzyme Corp Metodos mejorados para purificacion de vecotres de aav recombinantes.
US20150275195A1 (en) * 2012-10-24 2015-10-01 Genzyme Corporation Elution of biomolecules from multi-modal resins using mes and mops as mobile phase modifiers
CN104232687A (zh) * 2014-09-17 2014-12-24 许瑞安 一种重组腺相关病毒rAAV载体的分离纯化方法
EP3054007A1 (en) * 2015-02-09 2016-08-10 Institut National De La Sante Et De La Recherche Medicale (Inserm) Recombinant adeno-associated virus particle purification comprising an immuno-affinity purification step
EP3387117B1 (en) * 2015-12-11 2022-11-23 The Trustees Of The University Of Pennsylvania Scalable purification method for aav8
WO2017160360A2 (en) 2015-12-11 2017-09-21 The Trustees Of The University Of Pennsylvania Scalable purification method for aav9
CN115315306A (zh) 2019-11-21 2022-11-08 Emd密理博公司 亲水性膜

Also Published As

Publication number Publication date
JP2023089298A (ja) 2023-06-27
JP2021508484A (ja) 2021-03-11
WO2019133677A1 (en) 2019-07-04
KR20200106045A (ko) 2020-09-10
CA3084827A1 (en) 2019-07-04
US20200332266A1 (en) 2020-10-22
RU2020124946A (ru) 2022-01-31
BR112020013197A8 (pt) 2022-11-08
US11981934B2 (en) 2024-05-14
EP3731863A4 (en) 2021-10-27
EP3731863A1 (en) 2020-11-04
JP7299896B2 (ja) 2023-06-28
IL275533A (en) 2020-08-31
CN111655285A (zh) 2020-09-11
RU2020124946A3 (es) 2022-02-08
AU2018395254A1 (en) 2020-06-25
SG11202006232SA (en) 2020-07-29
BR112020013197A2 (pt) 2021-02-17

Similar Documents

Publication Publication Date Title
MX2020006884A (es) Metodos de purificacion de virus adenoasociado.
AU2018260998A1 (en) Modulatory polynucleotides
MX2019000962A (es) Novedosas proteinas de la capside del virus adenoasociado.
MX2020000216A (es) Metodos de purificacion por columna de vectores aav.
TN2018000005A1 (en) Agents, uses and methods for the treatment of synucleinopathy
EP3758724A4 (en) NOVEL ADENO-ASSOCIATED VIRUS (AAV) VECTORS, AAV VECTORS WITH REDUCED CAPSID DEAMIDATION AND USES THEREOF
WO2016018740A8 (en) Purification platform for bispecific antibodies
MX2019013172A (es) Composiciones y metodos para tratar la enfermedad de huntington.
EP3768695A4 (en) NOVEL ADENO-ASSOCIATED VIRUS (AAV) VECTORS, AAV VECTORS WITH REDUCED CAPSIID DEAMIDATION AND USES THEREOF
MX2019004784A (es) Metodos de purificacion del virus adeno-asociado.
WO2016191643A3 (en) Tigit-binding agents and uses thereof
WO2014164959A3 (en) Anti-il-33 antibodies and uses thereof
WO2017106684A3 (en) Antibodies specifically binding hla-dr and their uses
WO2016012285A3 (en) Method
PH12018502104A1 (en) Column-based fully scalable raav manufacturing process
MX2019005594A (es) Agentes, usos y metodos para el tratamiento de la sinucleinopatia.
MX2019013151A (es) Composiciones y metodos para expresar otoferlina.
EP3990031A4 (en) METHODS FOR PURIFYING ADENO-ASSOCIATED VIRUS
MX2016017115A (es) Metodos y reactivos para la purificacion de proteinas.
WO2017006052A3 (fr) UTILISATION DE FRAGMENTS Fc MODIFIÉS EN IMMUNOTHÉRAPIE
EP4273165A3 (en) Interferon beta antibodies and uses thereof
EP3575398A4 (en) ADENO-ASSOCIATED VIRUS (AAV) CAPSIDE PROTEIN MUTANT
AU2020299026A8 (en) Methods and AAV vectors for in vivo transduction
MX2017010883A (es) Composicion para el tratamiento de la infertilidad.
EP3846791A4 (en) ILLUDIN ANALOGUES, THEIR USES AND PROCEDURES FOR THEIR SYNTHESIS

Legal Events

Date Code Title Description
GB Transfer or rights

Owner name: TAKEDA PHARMACEUTICAL COMPANY LIMITED