MX2023005218A - Metodos de purificacion de vectores de aav mediante cromatografia de intercambio anionico. - Google Patents

Metodos de purificacion de vectores de aav mediante cromatografia de intercambio anionico.

Info

Publication number
MX2023005218A
MX2023005218A MX2023005218A MX2023005218A MX2023005218A MX 2023005218 A MX2023005218 A MX 2023005218A MX 2023005218 A MX2023005218 A MX 2023005218A MX 2023005218 A MX2023005218 A MX 2023005218A MX 2023005218 A MX2023005218 A MX 2023005218A
Authority
MX
Mexico
Prior art keywords
exchange chromatography
purification
methods
anion exchange
aav vectors
Prior art date
Application number
MX2023005218A
Other languages
English (en)
Inventor
Alexander Berrill
William S Kish
John R Lightholder
Matthew K Roach
William B Wellborn
Tamara Zekovic
Original Assignee
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer filed Critical Pfizer
Publication of MX2023005218A publication Critical patent/MX2023005218A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/10—Selective adsorption, e.g. chromatography characterised by constructional or operational features
    • B01D15/16—Selective adsorption, e.g. chromatography characterised by constructional or operational features relating to the conditioning of the fluid carrier
    • B01D15/166—Fluid composition conditioning, e.g. gradient
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
    • B01D15/361—Ion-exchange
    • B01D15/363—Anion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14141—Use of virus, viral particle or viral elements as a vector
    • C12N2750/14143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material

Abstract

La presente descripción proporciona métodos para purificar un vector de AAV recombinante (AAVr) de una solución mediante cromatografía de intercambio aniónico (AEX) para producir un eluato enriquecido para las cápsides completas y sin cápsides vacías.
MX2023005218A 2020-11-03 2021-11-01 Metodos de purificacion de vectores de aav mediante cromatografia de intercambio anionico. MX2023005218A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US202063109049P 2020-11-03 2020-11-03
US202163217194P 2021-06-30 2021-06-30
US202163253215P 2021-10-07 2021-10-07
PCT/IB2021/060095 WO2022097008A1 (en) 2020-11-03 2021-11-01 Methods for purification of aav vectors by anion exchange chromatography

Publications (1)

Publication Number Publication Date
MX2023005218A true MX2023005218A (es) 2023-05-16

Family

ID=78599082

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2023005218A MX2023005218A (es) 2020-11-03 2021-11-01 Metodos de purificacion de vectores de aav mediante cromatografia de intercambio anionico.

Country Status (8)

Country Link
US (1) US20230399656A1 (es)
EP (1) EP4240861A1 (es)
JP (1) JP2023548816A (es)
AU (1) AU2021375404A1 (es)
CA (1) CA3200401A1 (es)
IL (1) IL302608A (es)
MX (1) MX2023005218A (es)
WO (1) WO2022097008A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
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WO2024003687A1 (en) 2022-06-28 2024-01-04 Pfizer Inc. Nucleic acids encoding acid alpha-glucosidase (gaa) and vectors for gene therapy
WO2024038365A1 (en) * 2022-08-16 2024-02-22 Pfizer Inc. Methods for purification of aav vectors by anion exchange chromatography
WO2024081551A1 (en) * 2022-10-10 2024-04-18 Passage Bio, Inc. Method of purifying full recombinant aav particles

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Also Published As

Publication number Publication date
IL302608A (en) 2023-07-01
JP2023548816A (ja) 2023-11-21
US20230399656A1 (en) 2023-12-14
CA3200401A1 (en) 2022-05-12
EP4240861A1 (en) 2023-09-13
WO2022097008A1 (en) 2022-05-12
AU2021375404A1 (en) 2023-06-08

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