JP2020534872A5 - - Google Patents

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JP2020534872A5
JP2020534872A5 JP2020538759A JP2020538759A JP2020534872A5 JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5 JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020538759 A JP2020538759 A JP 2020538759A JP 2020534872 A5 JP2020534872 A5 JP 2020534872A5
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cell
perturbation
functionalized
beads
nucleotide
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JP2020538759A
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JP2020534872A (ja
JP7361700B2 (ja
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Priority claimed from PCT/US2018/052497 external-priority patent/WO2019060857A1/en
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JP2020538759A 2017-09-25 2018-09-24 オリゴヌクレオチドにコードされた化学ライブラリ Active JP7361700B2 (ja)

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JP2023171925A JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ

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US201762562905P 2017-09-25 2017-09-25
US201762562912P 2017-09-25 2017-09-25
US62/562,912 2017-09-25
US62/562,905 2017-09-25
PCT/US2018/052497 WO2019060857A1 (en) 2017-09-25 2018-09-24 CHEMICAL LIBRARIES CODED BY OLIGONUCLEOTIDES

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JP2020534872A JP2020534872A (ja) 2020-12-03
JP2020534872A5 true JP2020534872A5 (cg-RX-API-DMAC7.html) 2021-11-04
JP7361700B2 JP7361700B2 (ja) 2023-10-16

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JP2023171925A Pending JP2024001119A (ja) 2017-09-25 2023-10-03 オリゴヌクレオチドにコードされた化学ライブラリ

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US (8) US11084037B2 (cg-RX-API-DMAC7.html)
EP (1) EP3687687A4 (cg-RX-API-DMAC7.html)
JP (2) JP7361700B2 (cg-RX-API-DMAC7.html)
KR (2) KR102671480B1 (cg-RX-API-DMAC7.html)
CN (2) CN111971124B (cg-RX-API-DMAC7.html)
CA (1) CA3076747A1 (cg-RX-API-DMAC7.html)
IL (1) IL273511A (cg-RX-API-DMAC7.html)
WO (2) WO2019060830A1 (cg-RX-API-DMAC7.html)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10258590B2 (en) 2015-10-14 2019-04-16 Alcresta Therapeutics, Inc. Enteral feeding device and related methods of use
EP3472351B1 (de) 2016-06-15 2020-10-28 Ludwig-Maximilians-Universität München Einzelmolekülnachweis bzw. -quantifizierung durch dna-nanotechnologie
CA3076747A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. Oligonucleotide encoded chemical libraries
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
GB201817321D0 (en) * 2018-10-24 2018-12-05 Nanna Therapeutics Ltd Microbeads for tagless encoded chemical library screening
CN110359097B (zh) * 2019-07-08 2022-09-20 上海药明康德新药开发有限公司 DNA编码化合物库构建中通过Suzuki偶联反应得到On-DNA芳烃化合物的方法
CN110331447B (zh) * 2019-07-08 2022-05-27 上海药明康德新药开发有限公司 DNA编码化合物库构建中制备On-DNA芳基炔烃化合物的方法
US20220403374A1 (en) * 2019-09-03 2022-12-22 Flexomics, Llc Optically readable barcodes and systems and methods for characterizing molecular interactions
AU2020361681A1 (en) 2019-10-10 2022-05-05 1859, Inc. Methods and systems for microfluidic screening
WO2021113330A1 (en) * 2019-12-06 2021-06-10 Bio-Rad Laboratories, Inc. Sample processing barcoded bead composition, method, manufacturing, and system
GB201918340D0 (en) * 2019-12-12 2020-01-29 Cambridge Entpr Ltd Spatial barcoding
CN115698282A (zh) 2020-01-13 2023-02-03 福路伦特生物科学公司 单细胞测序
US20230065224A1 (en) * 2020-01-28 2023-03-02 Plexium, Inc. Oligonucleotide encoded chemical libraries, related systems, devices, and methods for detecting, analyzing, quantifying, and testing biologics/genetics
US11305288B2 (en) 2020-01-28 2022-04-19 Plexium, Inc. Assay devices for combinatorial libraries
US11027272B1 (en) 2020-01-28 2021-06-08 Plexium, Inc. Transfer dispensers for assay devices
US11040343B1 (en) 2020-01-28 2021-06-22 Plexium, Inc. Caps for assay devices
WO2021188500A1 (en) 2020-03-16 2021-09-23 Fluent Biosciences Inc. Multi-omic analysis in monodisperse droplets
EP4127058A4 (en) 2020-03-24 2024-04-24 Fluent Biosciences Inc. VIRAL DETECTION USING MATRIX EMULSIFICATION
JP7548305B2 (ja) * 2020-05-25 2024-09-10 日産化学株式会社 切断可能なdnaコード化ライブラリ
US20230249178A1 (en) * 2020-07-08 2023-08-10 Roche Sequencing Solutions, Inc. Split-pool synthesis apparatus and methods of performing split-pool synthesis
JP2023534028A (ja) 2020-07-15 2023-08-07 フルーエント バイオサイエンシーズ インコーポレイテッド 段階的ライゲーション用オリゴ
WO2022051369A1 (en) * 2020-09-01 2022-03-10 Replay Holdings, Llc Methods and systems for nucleic acid synthesis
WO2022220843A1 (en) * 2021-04-16 2022-10-20 Plexium, Inc. Caps for assay devices
JP2024516375A (ja) * 2021-04-16 2024-04-15 プレクシアム・インコーポレイテッド 組合せライブラリ用分析装置
CA3220008A1 (en) 2021-06-07 2022-12-15 Plexium, Inc. Transfer dispensers for assay devices with bead size exclusion
CN113403690B (zh) * 2021-06-21 2022-07-19 吉林大学 一种dna编码化合物库药物分子垂钓方法
WO2022271954A1 (en) 2021-06-24 2022-12-29 Illumina, Inc. Methods and compositions for combinatorial indexing of bead-based nucleic acids
WO2023287957A1 (en) * 2021-07-15 2023-01-19 Fluent Biosciences, Inc. Pre-templated instant partitioning of dna-encoded libraries
CN113549676B (zh) * 2021-08-24 2022-12-27 重庆范德瓦尔斯生物科技有限公司 一种基于磁珠的RNA pull down新方法
US20240393342A1 (en) * 2021-09-27 2024-11-28 The University Of Tokyo Cell labeling molecule and method for analyzing cell
US20250215615A1 (en) * 2021-11-24 2025-07-03 Nissan Chemical Corporation Method for evaluating dna-encoded library
WO2023108090A2 (en) * 2021-12-09 2023-06-15 University Of Florida Research Foundation, Incorporated Platform using dna-encoded small molecule libraries and rna selection to design small molecules that target rna
EP4444910A4 (en) 2021-12-10 2025-11-05 Bio Rad Laboratories Inc Compositions, processes and systems for the treatment of samples with functionalized particles of adjustable morphology
US20250197937A1 (en) * 2022-03-13 2025-06-19 Eleven Therapeutics Ltd System and method for high throughput evaluation of chemical modifications of nucleic acid molecules
WO2025159785A1 (en) * 2024-01-23 2025-07-31 Zafrens Inc. Perturbation beads for use in assays
CN115058492B (zh) * 2022-07-07 2025-09-19 东南大学 一种基于均分组合编码微球的空间转录组测序文库构建方法
WO2024091949A1 (en) * 2022-10-24 2024-05-02 1859, Inc. Methods and systems for encoded effector screening
US20250207122A1 (en) * 2022-12-29 2025-06-26 Illumina, Inc. Generation of a large library of barcoded beads using combinatorial split-pool strategy and chemical ligation
WO2024196941A1 (en) * 2023-03-20 2024-09-26 Fluid Discovery Core-shell gel particles for separating and analyzing rna and dna from a cell
US20250011843A1 (en) * 2023-07-07 2025-01-09 California Institute Of Technology Molecular detection using barcode probes or barcode fusion probes and hybridization chain reaction
WO2025085861A1 (en) * 2023-10-20 2025-04-24 Spectral Therapeutics, Inc. Composite particles for use in generating spectrally encoded libraries of chemical compounds, and methods of making and using the same

Family Cites Families (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573905A (en) * 1992-03-30 1996-11-12 The Scripps Research Institute Encoded combinatorial chemical libraries
CN1525171A (zh) 1993-10-01 2004-09-01 ŦԼ�и��ױ��Ǵ�ѧ���� 用标示物编码的多元组合化学库
EP0726906A4 (en) 1993-11-02 1998-10-07 Affymax Tech Nv THE SYNTHESIS AND EXAMINATION OF MOLECULAR DIVERSITY
ATE466109T1 (de) * 1995-06-07 2010-05-15 Solexa Inc Verfahren zur verbesserung der effizienz der polynukleotidsequenzierung
US5958703A (en) * 1996-12-03 1999-09-28 Glaxo Group Limited Use of modified tethers in screening compound libraries
DE69913509T2 (de) * 1998-05-15 2004-09-23 Oxford Gene Technology Ip Ltd., Kidlington Bibliotheken von Oligomeren, die mit unterschiedlichen Markierungen (TAGS) markiert sind
DE10050085C1 (de) 2000-10-10 2001-10-31 Jochem Koetting Verschlußelement und Verschlußsystem für Behältnisse und Gefäße
AU2003225552A1 (en) * 2002-02-05 2003-09-02 Genome Therapeutics Corporation Seal for microtiter plate and methods of use thereof
SE526038C2 (sv) * 2002-07-08 2005-06-21 Gambro Lundia Ab Polymeraffinitetsmatris, förfarande för framställning därav och anvädning därav
US8597597B2 (en) * 2003-06-26 2013-12-03 Seng Enterprises Ltd. Picoliter well holding device and method of making the same
EP2662136A3 (en) * 2003-08-27 2013-12-25 President and Fellows of Harvard College Method for handling and mixing droplets
CN101189345A (zh) * 2005-02-01 2008-05-28 Ab先进基因分析公司 珠基测序的试剂、方法和文库
US20070065844A1 (en) * 2005-06-08 2007-03-22 Massachusetts Institute Of Technology Solution-based methods for RNA expression profiling
DE102006028101B4 (de) 2006-06-19 2014-02-13 Siemens Aktiengesellschaft Verfahren zur Analyse von amplifizierten Nukleinsäuren
CN100417730C (zh) * 2006-10-10 2008-09-10 东南大学 基于引物延伸的dna测序方法
EP2222832A4 (en) * 2007-11-15 2012-08-29 Seng Entpr Ltd PICOLITER WELL SUPPORT AND METHOD FOR THE PRODUCTION THEREOF
WO2009126290A2 (en) * 2008-04-09 2009-10-15 Cornell University Coferons and methods of making and using them
US8741558B2 (en) 2009-02-04 2014-06-03 Wake Forest University Compositions, methods, and kits for identifying candidate molecules from encoded chemical libraries
US9347092B2 (en) * 2009-02-25 2016-05-24 Roche Molecular System, Inc. Solid support for high-throughput nucleic acid analysis
JP5985390B2 (ja) * 2009-04-02 2016-09-06 フリューダイム・コーポレイション 標的核酸にバーコードを付加するためのマルチプライマー増幅方法
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods
AU2010266010B2 (en) * 2009-06-26 2015-08-20 President And Fellows Of Harvard College Fluid injection
EP3561159B1 (en) * 2010-10-08 2023-09-20 President and Fellows of Harvard College High-throughput single cell barcoding
CN102485979B (zh) * 2010-12-02 2014-02-19 深圳华大基因科技服务有限公司 Ffpe样品核酸文库,其构建方法和ffpe样品分析方法
US20130344473A1 (en) * 2011-03-09 2013-12-26 Seng Enterprises Ltd. Arresting objects
WO2012162267A2 (en) * 2011-05-20 2012-11-29 Fluidigm Corporation Nucleic acid encoding reactions
US20140378345A1 (en) * 2012-08-14 2014-12-25 10X Technologies, Inc. Compositions and methods for sample processing
US10436778B2 (en) * 2013-03-15 2019-10-08 Plexbio Co., Ltd. Methods and systems for multiplex assays
DE202013003390U1 (de) 2013-04-11 2014-07-14 Brand Gmbh + Co Kg Pipettiervorrichtung mit einer Mikrodosiereinheit
EP3008201B1 (en) 2013-06-12 2019-08-07 The General Hospital Corporation Methods for multiplexed detection of target molecules and uses thereof
KR102436171B1 (ko) 2013-06-27 2022-08-24 10엑스 제노믹스, 인크. 샘플 처리를 위한 조성물 및 방법
KR102207770B1 (ko) * 2013-08-05 2021-01-26 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
EP2921556A1 (en) * 2014-03-21 2015-09-23 Lexogen GmbH Copy number preserving RNA analysis method
CN107075543B (zh) * 2014-04-21 2021-11-16 哈佛学院院长及董事 用于条形码化核酸的系统和方法
EP3169812A4 (en) * 2014-07-18 2017-12-20 CDI Laboratories Inc. Methods and compositions to identify, quantify, and characterize target analytes and binding moieties
EP4105337A1 (en) * 2014-09-09 2022-12-21 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
DK3262059T3 (da) 2015-02-24 2022-01-31 Hope City Platforme til screening af kemisk kodede, rumligt adresserede biblioteker
EP3277843A2 (en) * 2015-03-30 2018-02-07 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CA2983932C (en) * 2015-05-11 2023-07-25 Illumina, Inc. Platform for discovery and analysis of therapeutic agents
US20190046985A1 (en) 2015-09-17 2019-02-14 The Regents Of The University Of California Droplet-trapping devices for bioassays and diagnostics
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
JP7280181B2 (ja) 2016-10-01 2023-05-23 バークレー ライツ,インコーポレイテッド Dnaバーコード組成物及びマイクロ流体デバイスによるインサイチュ同定法
JP2020503846A (ja) * 2016-11-08 2020-02-06 ナンナ セラピューティクス リミテッドNanna Therapeutics Limited タグなしコード化化学ライブラリ
WO2018089641A2 (en) 2016-11-10 2018-05-17 The Scripps Research Institute Encode solid phase compound library with polynucleotide based barcoding
CA3076747A1 (en) 2017-09-25 2019-03-28 Plexium, Inc. Oligonucleotide encoded chemical libraries
US11584956B2 (en) * 2018-12-21 2023-02-21 Microsoft Technology Licensing, Llc Selectively controllable cleavable linkers

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