JP2020529221A5 - - Google Patents
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- Publication number
- JP2020529221A5 JP2020529221A5 JP2020527858A JP2020527858A JP2020529221A5 JP 2020529221 A5 JP2020529221 A5 JP 2020529221A5 JP 2020527858 A JP2020527858 A JP 2020527858A JP 2020527858 A JP2020527858 A JP 2020527858A JP 2020529221 A5 JP2020529221 A5 JP 2020529221A5
- Authority
- JP
- Japan
- Prior art keywords
- fusion
- protein
- deep
- fusion protein
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108020001507 fusion proteins Proteins 0.000 claims 24
- 102000037865 fusion proteins Human genes 0.000 claims 24
- 230000004927 fusion Effects 0.000 claims 17
- 102000004169 proteins and genes Human genes 0.000 claims 16
- 108090000623 proteins and genes Proteins 0.000 claims 16
- 238000003776 cleavage reaction Methods 0.000 claims 11
- 230000007017 scission Effects 0.000 claims 11
- 125000003275 alpha amino acid group Chemical group 0.000 claims 8
- 102000004877 Insulin Human genes 0.000 claims 6
- 108090001061 Insulin Proteins 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- 210000004027 cell Anatomy 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- 239000003623 enhancer Substances 0.000 claims 2
- 238000000034 method Methods 0.000 claims 2
- 102000039446 nucleic acids Human genes 0.000 claims 2
- 108020004707 nucleic acids Proteins 0.000 claims 2
- 150000007523 nucleic acids Chemical class 0.000 claims 2
- 229920001184 polypeptide Polymers 0.000 claims 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims 2
- 108091005804 Peptidases Proteins 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 210000004899 c-terminal region Anatomy 0.000 claims 1
- 238000010367 cloning Methods 0.000 claims 1
- 125000000487 histidyl group Chemical group [H]N([H])C(C(=O)O*)C([H])([H])C1=C([H])N([H])C([H])=N1 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- 239000011347 resin Substances 0.000 claims 1
- 229920005989 resin Polymers 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762538505P | 2017-07-28 | 2017-07-28 | |
| US62/538,505 | 2017-07-28 | ||
| PCT/US2018/044156 WO2019023616A1 (en) | 2017-07-28 | 2018-07-27 | FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2020529221A JP2020529221A (ja) | 2020-10-08 |
| JP2020529221A5 true JP2020529221A5 (enExample) | 2021-09-02 |
Family
ID=63312460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020527858A Pending JP2020529221A (ja) | 2017-07-28 | 2018-07-27 | 組み換えタンパク質発現のための融合タグ |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US12473340B2 (enExample) |
| EP (1) | EP3658574A1 (enExample) |
| JP (1) | JP2020529221A (enExample) |
| KR (1) | KR20200037819A (enExample) |
| CN (1) | CN111132996A (enExample) |
| AU (1) | AU2018307960B2 (enExample) |
| CA (1) | CA3071419A1 (enExample) |
| MX (1) | MX2020001058A (enExample) |
| SG (1) | SG11202000775SA (enExample) |
| WO (1) | WO2019023616A1 (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20200037819A (ko) | 2017-07-28 | 2020-04-09 | 더 트러스티즈 오브 프린스턴 유니버시티 | 재조합 단백질 발현을 위한 융합 태그 |
| WO2020252266A1 (en) * | 2019-06-14 | 2020-12-17 | Mytide Therapeutics, Inc. | A manufacturing process for peptide and protein production |
| WO2022094146A1 (en) | 2020-10-28 | 2022-05-05 | The Trustees Of Princeton University | Methods of converting precursor proteins to mature proteins using kex2 proteases |
| CN116981680A (zh) * | 2021-01-14 | 2023-10-31 | 生物催化剂有限责任公司 | 用于肽生产的组合物和方法 |
| CN113388009B (zh) * | 2021-05-18 | 2022-07-19 | 中国科学院福建物质结构研究所 | 一种标签蛋白及其编码基因、重组载体与应用 |
| KR102868986B1 (ko) * | 2022-09-27 | 2025-10-15 | 크리포 주식회사 | 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1996000787A1 (en) | 1994-06-30 | 1996-01-11 | Novo Nordisk Biotech, Inc. | Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein |
| US8153395B2 (en) | 2006-11-22 | 2012-04-10 | Novo Nordisk A/S | Method of making activated carboxypeptidases |
| ES2665853T3 (es) | 2012-03-29 | 2018-04-27 | Biocon Limited | Secreción de insulina glargina funcional directamente en el medio de cultivo mediante la sobreexpresión intracelularmente de Kex2p |
| CN103031328B (zh) * | 2012-11-22 | 2015-08-05 | 江南大学 | 利用一种融合标签提高外源蛋白质的表达量 |
| CN105324397B (zh) | 2013-03-14 | 2020-01-14 | 印第安纳大学研究及科技有限公司 | 胰岛素-肠促胰岛素缀合物 |
| SG11201600684XA (en) * | 2013-07-31 | 2016-02-26 | Biogenomics Ltd | Process for production of insulin and insulin analogues |
| EP3362468A1 (en) * | 2015-10-13 | 2018-08-22 | The Research Foundation for the State University of New York | Cleavable fusion tag for protein overexpression and purification |
| KR20200037819A (ko) | 2017-07-28 | 2020-04-09 | 더 트러스티즈 오브 프린스턴 유니버시티 | 재조합 단백질 발현을 위한 융합 태그 |
-
2018
- 2018-07-27 KR KR1020207005772A patent/KR20200037819A/ko not_active Withdrawn
- 2018-07-27 CN CN201880062847.9A patent/CN111132996A/zh active Pending
- 2018-07-27 AU AU2018307960A patent/AU2018307960B2/en not_active Expired - Fee Related
- 2018-07-27 EP EP18759004.7A patent/EP3658574A1/en not_active Withdrawn
- 2018-07-27 CA CA3071419A patent/CA3071419A1/en active Pending
- 2018-07-27 JP JP2020527858A patent/JP2020529221A/ja active Pending
- 2018-07-27 WO PCT/US2018/044156 patent/WO2019023616A1/en not_active Ceased
- 2018-07-27 MX MX2020001058A patent/MX2020001058A/es unknown
- 2018-07-27 SG SG11202000775SA patent/SG11202000775SA/en unknown
- 2018-07-27 US US16/633,991 patent/US12473340B2/en active Active
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