AU2018307960B2 - Fusion tags for recombinant protein expression - Google Patents

Fusion tags for recombinant protein expression Download PDF

Info

Publication number
AU2018307960B2
AU2018307960B2 AU2018307960A AU2018307960A AU2018307960B2 AU 2018307960 B2 AU2018307960 B2 AU 2018307960B2 AU 2018307960 A AU2018307960 A AU 2018307960A AU 2018307960 A AU2018307960 A AU 2018307960A AU 2018307960 B2 AU2018307960 B2 AU 2018307960B2
Authority
AU
Australia
Prior art keywords
protein
deep
fusion
amino acid
insulin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
AU2018307960A
Other languages
English (en)
Other versions
AU2018307960A1 (en
Inventor
Michael Hecht
Shlomo ZARZHITSKY
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Princeton University
Original Assignee
Princeton University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Princeton University filed Critical Princeton University
Publication of AU2018307960A1 publication Critical patent/AU2018307960A1/en
Application granted granted Critical
Publication of AU2018307960B2 publication Critical patent/AU2018307960B2/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2018307960A 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression Expired - Fee Related AU2018307960B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
US62/538,505 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS

Publications (2)

Publication Number Publication Date
AU2018307960A1 AU2018307960A1 (en) 2020-02-27
AU2018307960B2 true AU2018307960B2 (en) 2023-03-23

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2018307960A Expired - Fee Related AU2018307960B2 (en) 2017-07-28 2018-07-27 Fusion tags for recombinant protein expression

Country Status (10)

Country Link
US (1) US12473340B2 (enExample)
EP (1) EP3658574A1 (enExample)
JP (1) JP2020529221A (enExample)
KR (1) KR20200037819A (enExample)
CN (1) CN111132996A (enExample)
AU (1) AU2018307960B2 (enExample)
CA (1) CA3071419A1 (enExample)
MX (1) MX2020001058A (enExample)
SG (1) SG11202000775SA (enExample)
WO (1) WO2019023616A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200037819A (ko) 2017-07-28 2020-04-09 더 트러스티즈 오브 프린스턴 유니버시티 재조합 단백질 발현을 위한 융합 태그
WO2020252266A1 (en) * 2019-06-14 2020-12-17 Mytide Therapeutics, Inc. A manufacturing process for peptide and protein production
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
CN116981680A (zh) * 2021-01-14 2023-10-31 生物催化剂有限责任公司 用于肽生产的组合物和方法
CN113388009B (zh) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用
KR102868986B1 (ko) * 2022-09-27 2025-10-15 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US8153395B2 (en) 2006-11-22 2012-04-10 Novo Nordisk A/S Method of making activated carboxypeptidases
ES2665853T3 (es) 2012-03-29 2018-04-27 Biocon Limited Secreción de insulina glargina funcional directamente en el medio de cultivo mediante la sobreexpresión intracelularmente de Kex2p
CN103031328B (zh) * 2012-11-22 2015-08-05 江南大学 利用一种融合标签提高外源蛋白质的表达量
CN105324397B (zh) 2013-03-14 2020-01-14 印第安纳大学研究及科技有限公司 胰岛素-肠促胰岛素缀合物
SG11201600684XA (en) * 2013-07-31 2016-02-26 Biogenomics Ltd Process for production of insulin and insulin analogues
EP3362468A1 (en) * 2015-10-13 2018-08-22 The Research Foundation for the State University of New York Cleavable fusion tag for protein overexpression and purification
KR20200037819A (ko) 2017-07-28 2020-04-09 더 트러스티즈 오브 프린스턴 유니버시티 재조합 단백질 발현을 위한 융합 태그

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
AJAMALUDDIN MALIK: "Protein fusion tags for efficient expression and purification of recombinant proteins in the periplasmic space of E. coli", 3 BIOTECH, vol. 6, no. 1, 4 February 2016 (2016-02-04) *
KAMTEKAR S ET AL, "PROTEIN DESIGN BY BINARY PATTERNING OF POLAR AND NONPOLAR AMINO ACIDS", SCIENCE, AMERICAN ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE, US, (1993-12-10), vol. 262, no. 5140, pages 1680 - 1685 *
WEI YINAN ET AL: "Stably folded de novo proteins from a designedcombinatorial library", PROTEIN SCIENCE, WILEY, US, vol. 12, no. 1, 31 December 2002 (2002-12-31), pages 92 - 102 *
Y. WEI ET AL: "Enzyme-like proteins from an unselected library of designed amino acid sequences", PROTEIN ENGINEERING, DESIGN AND SELECTION, vol. 17, no. 1, 1 January 2004 (2004-01-01), GB, pages 67 - 75, *

Also Published As

Publication number Publication date
US20200216512A1 (en) 2020-07-09
JP2020529221A (ja) 2020-10-08
US12473340B2 (en) 2025-11-18
KR20200037819A (ko) 2020-04-09
MX2020001058A (es) 2020-08-06
WO2019023616A1 (en) 2019-01-31
CA3071419A1 (en) 2019-01-31
AU2018307960A1 (en) 2020-02-27
CN111132996A (zh) 2020-05-08
SG11202000775SA (en) 2020-02-27
EP3658574A1 (en) 2020-06-03

Similar Documents

Publication Publication Date Title
AU2018307960B2 (en) Fusion tags for recombinant protein expression
AU2012314355B2 (en) Split inteins and uses thereof
CA2237296C (en) Process for the preparation of peptides by way of streptavidin fusion proteins
CN104619726A (zh) 由超折叠绿色荧光蛋白构成的融合蛋白及其用途
KR20150008852A (ko) 계면활성제 펩타이드의 제조 방법
CN113195521A (zh) Mtu ΔI-CM内含肽变体和其应用
CN102666570B (zh) 新的肽基α-羟基甘氨酸α-酰胺化裂合酶
US8119369B2 (en) Human SUMO-3 for enhancing protein expression
KR102345011B1 (ko) GroES 융합을 이용한 글루카곤 유사 펩타이드-1 또는 이의 유사체의 생산방법
Abd Elhameed et al. Purification of proteins with native terminal sequences using a Ni (II)-cleavable C-terminal hexahistidine affinity tag
CN111073925B (zh) 一种基于无序蛋白偶联酶的高效多肽-多肽偶联系统和方法
JP2005514028A (ja) 融合タンパク質
KR20230165291A (ko) 폴리펩티드의 발현 증가를 위한 구축물 및 방법
US7223742B2 (en) Enhanced solubility of recombinant proteins
US7879578B2 (en) Self-assembled proteins and related methods and protein structures
CA2529282C (en) Recombinant igf expression systems
Greenshields et al. Strategies for recombinant expression of small, highly disulphide-bonded, cationic antimicrobial peptides
WO2022094146A1 (en) Methods of converting precursor proteins to mature proteins using kex2 proteases
WO2004022587A2 (en) A pair of polypeptides with specific and strong interaction
CN120943969A (zh) 一种肌醇光学探针及其制备方法和应用
EP2369000A1 (en) Production of peptides and proteins by accumulation in mitochondria

Legal Events

Date Code Title Description
MK25 Application lapsed reg. 22.2i(2) - failure to pay acceptance fee