KR20200037819A - 재조합 단백질 발현을 위한 융합 태그 - Google Patents

재조합 단백질 발현을 위한 융합 태그 Download PDF

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Publication number
KR20200037819A
KR20200037819A KR1020207005772A KR20207005772A KR20200037819A KR 20200037819 A KR20200037819 A KR 20200037819A KR 1020207005772 A KR1020207005772 A KR 1020207005772A KR 20207005772 A KR20207005772 A KR 20207005772A KR 20200037819 A KR20200037819 A KR 20200037819A
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South Korea
Prior art keywords
fusion
protein
deep
fusion protein
insulin
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KR1020207005772A
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English (en)
Korean (ko)
Inventor
마이클 헤흐트
쉴로모 자르지트스키
Original Assignee
더 트러스티즈 오브 프린스턴 유니버시티
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Publication of KR20200037819A publication Critical patent/KR20200037819A/ko
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Diabetes (AREA)
  • Endocrinology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020207005772A 2017-07-28 2018-07-27 재조합 단백질 발현을 위한 융합 태그 Withdrawn KR20200037819A (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762538505P 2017-07-28 2017-07-28
US62/538,505 2017-07-28
PCT/US2018/044156 WO2019023616A1 (en) 2017-07-28 2018-07-27 FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS

Publications (1)

Publication Number Publication Date
KR20200037819A true KR20200037819A (ko) 2020-04-09

Family

ID=63312460

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020207005772A Withdrawn KR20200037819A (ko) 2017-07-28 2018-07-27 재조합 단백질 발현을 위한 융합 태그

Country Status (10)

Country Link
US (1) US12473340B2 (enExample)
EP (1) EP3658574A1 (enExample)
JP (1) JP2020529221A (enExample)
KR (1) KR20200037819A (enExample)
CN (1) CN111132996A (enExample)
AU (1) AU2018307960B2 (enExample)
CA (1) CA3071419A1 (enExample)
MX (1) MX2020001058A (enExample)
SG (1) SG11202000775SA (enExample)
WO (1) WO2019023616A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024071960A1 (ko) * 2022-09-27 2024-04-04 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 his-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20200037819A (ko) 2017-07-28 2020-04-09 더 트러스티즈 오브 프린스턴 유니버시티 재조합 단백질 발현을 위한 융합 태그
WO2020252266A1 (en) * 2019-06-14 2020-12-17 Mytide Therapeutics, Inc. A manufacturing process for peptide and protein production
WO2022094146A1 (en) 2020-10-28 2022-05-05 The Trustees Of Princeton University Methods of converting precursor proteins to mature proteins using kex2 proteases
CN116981680A (zh) * 2021-01-14 2023-10-31 生物催化剂有限责任公司 用于肽生产的组合物和方法
CN113388009B (zh) * 2021-05-18 2022-07-19 中国科学院福建物质结构研究所 一种标签蛋白及其编码基因、重组载体与应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1996000787A1 (en) 1994-06-30 1996-01-11 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
US8153395B2 (en) 2006-11-22 2012-04-10 Novo Nordisk A/S Method of making activated carboxypeptidases
ES2665853T3 (es) 2012-03-29 2018-04-27 Biocon Limited Secreción de insulina glargina funcional directamente en el medio de cultivo mediante la sobreexpresión intracelularmente de Kex2p
CN103031328B (zh) * 2012-11-22 2015-08-05 江南大学 利用一种融合标签提高外源蛋白质的表达量
CN105324397B (zh) 2013-03-14 2020-01-14 印第安纳大学研究及科技有限公司 胰岛素-肠促胰岛素缀合物
SG11201600684XA (en) * 2013-07-31 2016-02-26 Biogenomics Ltd Process for production of insulin and insulin analogues
EP3362468A1 (en) * 2015-10-13 2018-08-22 The Research Foundation for the State University of New York Cleavable fusion tag for protein overexpression and purification
KR20200037819A (ko) 2017-07-28 2020-04-09 더 트러스티즈 오브 프린스턴 유니버시티 재조합 단백질 발현을 위한 융합 태그

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024071960A1 (ko) * 2022-09-27 2024-04-04 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 his-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법
KR20240043839A (ko) * 2022-09-27 2024-04-04 크리포 주식회사 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법

Also Published As

Publication number Publication date
US20200216512A1 (en) 2020-07-09
JP2020529221A (ja) 2020-10-08
US12473340B2 (en) 2025-11-18
MX2020001058A (es) 2020-08-06
WO2019023616A1 (en) 2019-01-31
CA3071419A1 (en) 2019-01-31
AU2018307960B2 (en) 2023-03-23
AU2018307960A1 (en) 2020-02-27
CN111132996A (zh) 2020-05-08
SG11202000775SA (en) 2020-02-27
EP3658574A1 (en) 2020-06-03

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