JP2020524490A - Ｅｓｃｈｅｒｉｃｈｉａ Ｃｏｌｉを改良するためのＨＴＰゲノム操作プラットフォーム - Google Patents

Ｅｓｃｈｅｒｉｃｈｉａ Ｃｏｌｉを改良するためのＨＴＰゲノム操作プラットフォーム Download PDF

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JP2020524490A
JP2020524490A JP2019567283A JP2019567283A JP2020524490A JP 2020524490 A JP2020524490 A JP 2020524490A JP 2019567283 A JP2019567283 A JP 2019567283A JP 2019567283 A JP2019567283 A JP 2019567283A JP 2020524490 A JP2020524490 A JP 2020524490A
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coli
gene
library
coli strain
strain
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JP2020524490A5 (enExample
Inventor
マシュー デイビス，
マシュー デイビス，
クリスティー ウィスニュースキー，
クリスティー ウィスニュースキー，
パトリック ウエストフォール，
パトリック ウエストフォール，
ザック サーバー，
ザック サーバー，
エリック ジェデディア ディーン，
エリック ジェデディア ディーン，
ショーン マンチェスター，
ショーン マンチェスター，
キャサリン ゴラ，
キャサリン ゴラ，
エリン シェルマン，
エリン シェルマン，
アーロン キンボール，
アーロン キンボール，
ショーン シィジカ，
ショーン シィジカ，
バーバラ フレウェン，
バーバラ フレウェン，
トーマス トレイナー，
トーマス トレイナー，
マイケル フラッシュマン，
マイケル フラッシュマン，
ロバート ハウシャルター，
ロバート ハウシャルター，
ステイシー−アン モーガン，
ステイシー−アン モーガン，
マイケル ブレイズ，
マイケル ブレイズ，
プラバ ラマクリシュナン，
プラバ ラマクリシュナン，
カイル ロスチャイルド−マンチネッリ，
カイル ロスチャイルド−マンチネッリ，
ヨンニュン キム，
ヨンニュン キム，
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Zymergen Inc
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Zymergen Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1079—Screening libraries by altering the phenotype or phenotypic trait of the host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

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  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Virology (AREA)
  • Ecology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
JP2019567283A 2017-06-06 2018-06-06 Ｅｓｃｈｅｒｉｃｈｉａ Ｃｏｌｉを改良するためのＨＴＰゲノム操作プラットフォーム Pending JP2020524490A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201762515870P 2017-06-06 2017-06-06
US62/515,870 2017-06-06
PCT/US2018/036333 WO2018226880A1 (en) 2017-06-06 2018-06-06 A htp genomic engineering platform for improving escherichia coli

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JP2020524490A true JP2020524490A (ja) 2020-08-20
JP2020524490A5 JP2020524490A5 (enExample) 2021-07-26

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US (1) US20200370058A1 (enExample)
EP (2) EP3485013B1 (enExample)
JP (1) JP2020524490A (enExample)
KR (1) KR20200026874A (enExample)
CN (1) CN110945125A (enExample)
CA (1) CA3064612A1 (enExample)
ES (1) ES2875579T3 (enExample)
WO (1) WO2018226880A1 (enExample)

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* Cited by examiner, † Cited by third party
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WO2022124302A1 (ja) * 2020-12-08 2022-06-16 国立大学法人神戸大学 有用化合物を高生産する代謝改変微生物株の構築方法及び代謝改変大腸菌株

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CN114921463B (zh) * 2022-04-11 2023-12-01 中国农业科学院生物技术研究所 一种调控萜类化合物合成产量的人工非编码RNA分子CsiY及其应用
CN115074410B (zh) * 2022-06-30 2025-08-19 武汉合生科技有限公司 一种生物合成虾青素的方法及载体
CN115992160A (zh) * 2022-09-30 2023-04-21 倍生生物科技(深圳)有限公司 一种穿梭载体及其构建方法和应用
CN115820703A (zh) * 2022-12-20 2023-03-21 山东乾肽生物科技有限公司 利用rna环化技术制备寡肽的方法及寡肽的表达dna
CN116013407B (zh) * 2022-12-26 2025-06-27 杭州智科慧创科技有限公司 一种基于语言模型的性质解耦蛋白质生成方法
CN116504313A (zh) * 2023-05-08 2023-07-28 暨南大学 基因终止子查询方法、装置、设备及可读存储介质
CN117275590B (zh) * 2023-11-10 2024-03-26 华东师范大学 有机固废体系中大分子的降解功能基因数据库与分析平台
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Cited By (2)

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CA3064612A1 (en) 2018-12-13
EP3485013B1 (en) 2021-03-24
ES2875579T3 (es) 2021-11-10
EP3485013A1 (en) 2019-05-22
WO2018226880A1 (en) 2018-12-13
US20200370058A1 (en) 2020-11-26
CN110945125A (zh) 2020-03-31
EP3878961A1 (en) 2021-09-15
KR20200026874A (ko) 2020-03-11

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