JP2020523034A5 - - Google Patents
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- JP2020523034A5 JP2020523034A5 JP2020515835A JP2020515835A JP2020523034A5 JP 2020523034 A5 JP2020523034 A5 JP 2020523034A5 JP 2020515835 A JP2020515835 A JP 2020515835A JP 2020515835 A JP2020515835 A JP 2020515835A JP 2020523034 A5 JP2020523034 A5 JP 2020523034A5
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- polynucleotide
- polynucleotides
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- vessel
- adapter
- Prior art date
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- 108091033319 polynucleotide Proteins 0.000 claims description 411
- 239000002157 polynucleotide Substances 0.000 claims description 411
- 102000040430 polynucleotide Human genes 0.000 claims description 411
- 238000000034 method Methods 0.000 claims description 208
- 210000004027 cell Anatomy 0.000 claims description 108
- 230000000295 complement effect Effects 0.000 claims description 90
- 108091034117 Oligonucleotide Proteins 0.000 claims description 89
- 239000013615 primer Substances 0.000 claims description 77
- 230000037452 priming Effects 0.000 claims description 59
- 125000003729 nucleotide group Chemical group 0.000 claims description 46
- 239000002773 nucleotide Substances 0.000 claims description 45
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims description 36
- 108091093088 Amplicon Proteins 0.000 claims description 20
- 108091008874 T cell receptors Proteins 0.000 claims description 18
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 18
- 102100034343 Integrase Human genes 0.000 claims description 17
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 17
- 238000012163 sequencing technique Methods 0.000 claims description 17
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 16
- 108060003951 Immunoglobulin Proteins 0.000 claims description 15
- 230000003321 amplification Effects 0.000 claims description 15
- 102000018358 immunoglobulin Human genes 0.000 claims description 15
- 108020004635 Complementary DNA Proteins 0.000 claims description 12
- 238000010804 cDNA synthesis Methods 0.000 claims description 12
- 239000002299 complementary DNA Substances 0.000 claims description 12
- 210000001744 T-lymphocyte Anatomy 0.000 claims description 10
- RWQNBRDOKXIBIV-UHFFFAOYSA-N thymine Chemical group CC1=CNC(=O)NC1=O RWQNBRDOKXIBIV-UHFFFAOYSA-N 0.000 claims description 10
- 239000007787 solid Substances 0.000 claims description 9
- 229930024421 Adenine Natural products 0.000 claims description 5
- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 5
- 229960000643 adenine Drugs 0.000 claims description 5
- GFFGJBXGBJISGV-UHFFFAOYSA-N adenyl group Chemical group N1=CN=C2N=CNC2=C1N GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 claims description 5
- 230000000694 effects Effects 0.000 claims description 5
- 210000004698 lymphocyte Anatomy 0.000 claims description 5
- 229940113082 thymine Drugs 0.000 claims description 5
- 108091028664 Ribonucleotide Proteins 0.000 claims description 4
- 210000003719 b-lymphocyte Anatomy 0.000 claims description 4
- 230000002934 lysing effect Effects 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 4
- 238000010839 reverse transcription Methods 0.000 claims description 4
- 239000002336 ribonucleotide Substances 0.000 claims description 4
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 4
- 238000011222 transcriptome analysis Methods 0.000 claims description 4
- 238000000137 annealing Methods 0.000 claims description 3
- 230000020411 cell activation Effects 0.000 claims description 3
- 230000003915 cell function Effects 0.000 claims description 3
- 230000002062 proliferating effect Effects 0.000 claims description 3
- 108091026890 Coding region Proteins 0.000 claims description 2
- 239000003155 DNA primer Substances 0.000 claims description 2
- 150000001413 amino acids Chemical class 0.000 claims description 2
- 230000001413 cellular effect Effects 0.000 claims description 2
- 230000009977 dual effect Effects 0.000 claims description 2
- 239000000839 emulsion Substances 0.000 claims description 2
- 238000009396 hybridization Methods 0.000 claims description 2
- 239000003094 microcapsule Substances 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 229920001184 polypeptide Polymers 0.000 claims description 2
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 2
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 2
- 102100029764 DNA-directed DNA/RNA polymerase mu Human genes 0.000 claims 1
- 238000002360 preparation method Methods 0.000 claims 1
- 108090000623 proteins and genes Proteins 0.000 claims 1
- 239000000523 sample Substances 0.000 description 13
- 210000003071 memory t lymphocyte Anatomy 0.000 description 6
- 102100033215 DNA nucleotidylexotransferase Human genes 0.000 description 4
- 210000002865 immune cell Anatomy 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 210000003819 peripheral blood mononuclear cell Anatomy 0.000 description 4
- 239000011324 bead Substances 0.000 description 3
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 210000003162 effector t lymphocyte Anatomy 0.000 description 2
- 239000008240 homogeneous mixture Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 210000003289 regulatory T cell Anatomy 0.000 description 2
- 241000271566 Aves Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 125000000217 alkyl group Chemical group 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 210000000612 antigen-presenting cell Anatomy 0.000 description 1
- 230000001363 autoimmune Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
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- 208000032839 leukemia Diseases 0.000 description 1
- 210000001806 memory b lymphocyte Anatomy 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 208000025113 myeloid leukemia Diseases 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762511949P | 2017-05-26 | 2017-05-26 | |
| US62/511,949 | 2017-05-26 | ||
| PCT/US2018/034768 WO2018218222A1 (en) | 2017-05-26 | 2018-05-25 | High-throughput polynucleotide library sequencing and transcriptome analysis |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020523034A JP2020523034A (ja) | 2020-08-06 |
| JP2020523034A5 true JP2020523034A5 (https=) | 2021-07-26 |
| JP7308187B2 JP7308187B2 (ja) | 2023-07-13 |
Family
ID=62621068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020515835A Active JP7308187B2 (ja) | 2017-05-26 | 2018-05-25 | ハイスループットポリヌクレオチドライブラリーシーケンシングおよびトランスクリプトーム解析法 |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US12049667B2 (https=) |
| EP (1) | EP3631012B1 (https=) |
| JP (1) | JP7308187B2 (https=) |
| KR (1) | KR102550778B1 (https=) |
| CN (1) | CN111148849A (https=) |
| AU (1) | AU2018273999A1 (https=) |
| CA (1) | CA3064205A1 (https=) |
| ES (1) | ES2926722T3 (https=) |
| MA (1) | MA49352A (https=) |
| WO (1) | WO2018218222A1 (https=) |
Families Citing this family (57)
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| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
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| CA3034924C (en) | 2016-09-26 | 2025-11-18 | Becton, Dickinson And Company | MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES |
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| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
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| KR20210141449A (ko) * | 2018-12-28 | 2021-11-23 | 내셔널 유니버시티 오브 싱가포르 | 표적화된 상보적 dna 강화를 위한 방법 |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP2022543278A (ja) * | 2019-08-05 | 2022-10-11 | ミッション バイオ インコーポレイテッド | 細胞軌道を測定するためのシングルセル分析のための方法及び装置 |
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| US12163189B2 (en) | 2019-11-27 | 2024-12-10 | University Of Washington | Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria |
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- 2018-05-25 CA CA3064205A patent/CA3064205A1/en active Pending
- 2018-05-25 KR KR1020197038441A patent/KR102550778B1/ko active Active
- 2018-05-25 MA MA049352A patent/MA49352A/fr unknown
- 2018-05-25 EP EP18731697.1A patent/EP3631012B1/en active Active
- 2018-05-25 CN CN201880049800.9A patent/CN111148849A/zh active Pending
- 2018-05-25 JP JP2020515835A patent/JP7308187B2/ja active Active
- 2018-05-25 WO PCT/US2018/034768 patent/WO2018218222A1/en not_active Ceased
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