JP2017527313A5 - - Google Patents
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- JP2017527313A5 JP2017527313A5 JP2017533733A JP2017533733A JP2017527313A5 JP 2017527313 A5 JP2017527313 A5 JP 2017527313A5 JP 2017533733 A JP2017533733 A JP 2017533733A JP 2017533733 A JP2017533733 A JP 2017533733A JP 2017527313 A5 JP2017527313 A5 JP 2017527313A5
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- polynucleotide
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- 108091033319 polynucleotide Proteins 0.000 claims description 570
- 102000040430 polynucleotide Human genes 0.000 claims description 570
- 239000002157 polynucleotide Substances 0.000 claims description 569
- 238000000034 method Methods 0.000 claims description 444
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- 108060003951 Immunoglobulin Proteins 0.000 claims description 38
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- 150000007523 nucleic acids Chemical class 0.000 claims description 27
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- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims description 24
- 238000012163 sequencing technique Methods 0.000 claims description 15
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- 108010008286 DNA nucleotidylexotransferase Proteins 0.000 claims description 11
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- 108020004635 Complementary DNA Proteins 0.000 claims 1
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- 229940113082 thymine Drugs 0.000 description 8
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- 201000009030 Carcinoma Diseases 0.000 description 2
- 208000005243 Chondrosarcoma Diseases 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 2
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- 241001465754 Metazoa Species 0.000 description 2
- 241000713869 Moloney murine leukemia virus Species 0.000 description 2
- 206010039491 Sarcoma Diseases 0.000 description 2
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- 208000032839 leukemia Diseases 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 210000001806 memory b lymphocyte Anatomy 0.000 description 2
- 108020004999 messenger RNA Proteins 0.000 description 2
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- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 1
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Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020036503A JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462050549P | 2014-09-15 | 2014-09-15 | |
| US62/050,549 | 2014-09-15 | ||
| US201462051832P | 2014-09-17 | 2014-09-17 | |
| US62/051,832 | 2014-09-17 | ||
| PCT/US2015/050119 WO2016044227A1 (en) | 2014-09-15 | 2015-09-15 | High-throughput nucleotide library sequencing |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020036503A Division JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017527313A JP2017527313A (ja) | 2017-09-21 |
| JP2017527313A5 true JP2017527313A5 (https=) | 2018-11-01 |
| JP6672310B2 JP6672310B2 (ja) | 2020-03-25 |
Family
ID=54291588
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017533733A Active JP6672310B2 (ja) | 2014-09-15 | 2015-09-15 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2020036503A Active JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A Active JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020036503A Active JP6960490B2 (ja) | 2014-09-15 | 2020-03-04 | ハイスループットヌクレオチドライブラリーシークエンシング |
| JP2021166730A Active JP7278352B2 (ja) | 2014-09-15 | 2021-10-11 | ハイスループットヌクレオチドライブラリーシークエンシング |
Country Status (14)
| Country | Link |
|---|---|
| US (3) | US10590483B2 (https=) |
| EP (3) | EP3950944A1 (https=) |
| JP (3) | JP6672310B2 (https=) |
| KR (1) | KR102541849B1 (https=) |
| CN (1) | CN107002076B (https=) |
| AU (2) | AU2015318011B2 (https=) |
| CA (1) | CA2961210A1 (https=) |
| ES (2) | ES2895750T3 (https=) |
| IL (1) | IL251123B (https=) |
| MX (1) | MX378730B (https=) |
| NZ (2) | NZ730044A (https=) |
| SG (2) | SG11201702060VA (https=) |
| WO (1) | WO2016044227A1 (https=) |
| ZA (1) | ZA201701916B (https=) |
Families Citing this family (142)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101829182B1 (ko) | 2009-04-02 | 2018-03-29 | 플루이다임 코포레이션 | 표적 핵산의 바코딩을 위한 멀티 프라이머 증폭 방법 |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| US10941396B2 (en) | 2012-02-27 | 2021-03-09 | Becton, Dickinson And Company | Compositions and kits for molecular counting |
| CA3216609C (en) | 2012-08-14 | 2024-05-14 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US10584381B2 (en) | 2012-08-14 | 2020-03-10 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US11591637B2 (en) | 2012-08-14 | 2023-02-28 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9701998B2 (en) | 2012-12-14 | 2017-07-11 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10273541B2 (en) | 2012-08-14 | 2019-04-30 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10323279B2 (en) | 2012-08-14 | 2019-06-18 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10752949B2 (en) | 2012-08-14 | 2020-08-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| CN108267581A (zh) | 2012-08-24 | 2018-07-10 | 耶鲁大学 | 用于高通量多重检测的系统、装置和方法 |
| WO2014093676A1 (en) | 2012-12-14 | 2014-06-19 | 10X Technologies, Inc. | Methods and systems for processing polynucleotides |
| US10533221B2 (en) | 2012-12-14 | 2020-01-14 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| AU2014214682B2 (en) | 2013-02-08 | 2018-07-26 | 10X Genomics, Inc. | Polynucleotide barcode generation |
| US10119134B2 (en) | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| ES2711168T3 (es) | 2013-08-28 | 2019-04-30 | Becton Dickinson Co | Análisis masivo en paralelo de células individuales |
| WO2015157567A1 (en) | 2014-04-10 | 2015-10-15 | 10X Genomics, Inc. | Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same |
| EP3155426B1 (en) | 2014-06-13 | 2023-07-19 | Immudex ApS | General detection and isolation of specific cells by binding of labeled molecules |
| CN113249435B (zh) | 2014-06-26 | 2024-09-03 | 10X基因组学有限公司 | 分析来自单个细胞或细胞群体的核酸的方法 |
| US12312640B2 (en) | 2014-06-26 | 2025-05-27 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| MX2016016904A (es) | 2014-06-26 | 2017-03-27 | 10X Genomics Inc | Analisis de secuencias de acidos nucleicos. |
| ES2895750T3 (es) | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
| MX2017005267A (es) | 2014-10-29 | 2017-07-26 | 10X Genomics Inc | Metodos y composiciones para la secuenciacion de acidos nucleicos seleccionados como diana. |
| US9975122B2 (en) | 2014-11-05 | 2018-05-22 | 10X Genomics, Inc. | Instrument systems for integrated sample processing |
| DE102014017085A1 (de) * | 2014-11-20 | 2016-05-25 | Kautex Textron Gmbh & Co. Kg | Vorrichtung zum Erzeugen einer Verstärkungsstruktur auf einer Formkörperoberfläche |
| CN112782140A (zh) | 2014-12-03 | 2021-05-11 | 伊索普莱西斯公司 | 细胞分泌特征的分析和筛选 |
| SG11201705615UA (en) | 2015-01-12 | 2017-08-30 | 10X Genomics Inc | Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same |
| EP4286516A3 (en) | 2015-02-24 | 2024-03-06 | 10X Genomics, Inc. | Partition processing methods and systems |
| BR112017018054A2 (pt) | 2015-02-24 | 2018-07-24 | 10X Genomics Inc | métodos para a cobertura de sequências de ácidos nucleicos direcionadas |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| SG11201708579WA (en) * | 2015-04-21 | 2017-11-29 | Gen Automation Lab Tech Inc | High resolution systems, kits, apparatus, and methods for high throughput microbiology applications |
| US10712356B2 (en) | 2015-04-21 | 2020-07-14 | General Automation Lab Technologies Inc. | Apparatus and method for picking biological sample |
| US10788452B2 (en) * | 2015-04-21 | 2020-09-29 | General Automation Lab Technologies Inc. | High resolution systems, kits, apparatus, and methods for bacterial community relationship determination and other high throughput microbiology applications |
| CN107580632B (zh) | 2015-04-23 | 2021-12-28 | 贝克顿迪金森公司 | 用于全转录组扩增的方法和组合物 |
| AU2016253964B2 (en) | 2015-04-27 | 2022-07-07 | Abvitro Llc | Methods of sequencing, determining, pairing, and validating therapeutic agents and disease specific antigens |
| CN108026524A (zh) | 2015-09-11 | 2018-05-11 | 赛卢拉研究公司 | 用于核酸文库标准化的方法和组合物 |
| AU2016326738B2 (en) | 2015-09-24 | 2023-08-31 | Abvitro Llc | HIV antibody compositions and methods of use |
| KR20180085717A (ko) | 2015-09-24 | 2018-07-27 | 에이비비트로, 엘엘씨 | 친화성-올리고뉴클레오타이드 콘쥬게이트 및 그것의 사용 |
| SG11201803646YA (en) | 2015-11-04 | 2018-05-30 | Atreca Inc | Combinatorial sets of nucleic acid barcodes for analysis of nucleic acids associated with single cells |
| US10265497B2 (en) | 2015-11-24 | 2019-04-23 | Massachusetts Institute Of Technology | Systems and methods for treating dementia |
| SG11201804086VA (en) | 2015-12-04 | 2018-06-28 | 10X Genomics Inc | Methods and compositions for nucleic acid analysis |
| RU2729116C2 (ru) | 2015-12-16 | 2020-08-04 | Гритстоун Онколоджи, Инк. | Идентификация, производство и применение неоантигенов |
| WO2017106777A1 (en) | 2015-12-16 | 2017-06-22 | Fluidigm Corporation | High-level multiplex amplification |
| WO2017197343A2 (en) | 2016-05-12 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic on-chip filters |
| WO2017197338A1 (en) | 2016-05-13 | 2017-11-16 | 10X Genomics, Inc. | Microfluidic systems and methods of use |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN109661474A (zh) * | 2016-07-14 | 2019-04-19 | 富鲁达公司 | 单细胞转录物测序 |
| EP3510398B1 (en) | 2016-09-12 | 2026-02-25 | Isoplexis Corporation | System and methods for multiplexed analysis of cellular and other immunotherapeutics |
| WO2018053362A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation |
| CA3037190A1 (en) | 2016-09-15 | 2018-03-22 | ArcherDX, Inc. | Methods of nucleic acid sample preparation for analysis of cell-free dna |
| BR112019005748A2 (pt) * | 2016-09-24 | 2019-06-18 | Abvitro Llc | afinidade-conjugados de oligonucleotídeo e usos destes |
| CA3034924C (en) | 2016-09-26 | 2025-11-18 | Becton, Dickinson And Company | MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES |
| CN110139873A (zh) | 2016-10-03 | 2019-08-16 | 朱诺治疗学股份有限公司 | Hpv特异性结合分子 |
| WO2018075693A1 (en) | 2016-10-19 | 2018-04-26 | 10X Genomics, Inc. | Methods and systems for barcoding nucleic acid molecules from individual cells or cell populations |
| EP4198140B1 (en) * | 2016-11-02 | 2024-09-04 | ArcherDX, LLC | Methods of nucleic acid sample preparation for immune repertoire sequencing |
| EP4036578A1 (en) | 2016-11-11 | 2022-08-03 | Isoplexis Corporation | Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells |
| KR20250153860A (ko) | 2016-11-17 | 2025-10-27 | 코그니토 쎄라퓨틱스, 인코포레이티드 | 시각 자극을 통한 신경 자극 방법 및 시스템 |
| EP3545104B1 (en) * | 2016-11-23 | 2021-02-24 | Université de Strasbourg | Tandem barcoding of target molecules for their absolute quantification at single-entity resolution |
| US10815525B2 (en) | 2016-12-22 | 2020-10-27 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10011872B1 (en) | 2016-12-22 | 2018-07-03 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US10550429B2 (en) | 2016-12-22 | 2020-02-04 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| JP7360324B2 (ja) | 2016-12-23 | 2023-10-12 | ビステラ， インコーポレイテッド | 結合ポリペプチドおよびそれを作製する方法 |
| US12264411B2 (en) | 2017-01-30 | 2025-04-01 | 10X Genomics, Inc. | Methods and systems for analysis |
| CN110214186B (zh) | 2017-01-30 | 2023-11-24 | 10X基因组学有限公司 | 用于基于微滴的单细胞条形编码的方法和系统 |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| CN110199022A (zh) * | 2017-02-16 | 2019-09-03 | 宝生物工程(美国) 有限公司 | 制备核酸文库的方法和用于实施所述方法的组合物和试剂盒 |
| WO2018208133A1 (ko) * | 2017-05-12 | 2018-11-15 | 서울대학교산학협력단 | 고순도 뉴클레오타이드 획득 방법 및 장치 |
| CN109526228B (zh) | 2017-05-26 | 2022-11-25 | 10X基因组学有限公司 | 转座酶可接近性染色质的单细胞分析 |
| ES2926722T3 (es) | 2017-05-26 | 2022-10-27 | Abvitro Llc | Secuenciación de alto rendimiento de biblioteca de polinucleótidos y análisis de transcriptoma |
| US10844372B2 (en) | 2017-05-26 | 2020-11-24 | 10X Genomics, Inc. | Single cell analysis of transposase accessible chromatin |
| JP7536450B2 (ja) | 2017-06-05 | 2024-08-20 | ベクトン・ディキンソン・アンド・カンパニー | 単一細胞用のサンプルインデックス付加 |
| US20200131564A1 (en) * | 2017-07-07 | 2020-04-30 | Board Of Regents, The University Of Texas System | High-coverage and ultra-accurate immune repertoire sequencing using molecular identifiers |
| CN111263641B (zh) | 2017-08-09 | 2025-10-31 | 朱诺治疗学股份有限公司 | 用于制备基因工程化细胞的方法和组合物 |
| EP3679370A1 (en) | 2017-09-07 | 2020-07-15 | Juno Therapeutics, Inc. | Methods of identifying cellular attributes related to outcomes associated with cell therapy |
| BR112020006643A2 (pt) | 2017-10-03 | 2020-09-24 | Juno Therapeutics Inc | moléculas de ligação específica ao hpv |
| KR20260039788A (ko) | 2017-10-10 | 2026-03-20 | 시애틀 프로젝트 코포레이션 | 핫스팟을 이용한 신생항원 동정 |
| KR102706443B1 (ko) | 2017-10-10 | 2024-09-11 | 메사추세츠 인스티튜트 오브 테크놀로지 | 치매를 예방, 경감 및/또는 치료하기 위한 시스템 및 방법 |
| US10960225B2 (en) | 2017-10-10 | 2021-03-30 | Massachusetts Institute Of Technology | Systems and methods for preventing, mitigating, and/or treating dementia via visual stimulation that binds higher order brain regions, reduces neurodegeneration and neuroinflammation, and improves cognitive function |
| EP3954782A1 (en) | 2017-11-15 | 2022-02-16 | 10X Genomics, Inc. | Functionalized gel beads |
| US10829815B2 (en) | 2017-11-17 | 2020-11-10 | 10X Genomics, Inc. | Methods and systems for associating physical and genetic properties of biological particles |
| CA3083097A1 (en) | 2017-11-22 | 2019-05-31 | Gritstone Oncology, Inc. | Reducing junction epitope presentation for neoantigens |
| EP3717661A1 (en) | 2017-11-27 | 2020-10-07 | The Trustees of Columbia University in the City of New York | Rna printing and sequencing devices, methods, and systems |
| WO2019113457A1 (en) | 2017-12-07 | 2019-06-13 | Massachusetts Institute Of Technology | Single cell analyses |
| WO2019118937A1 (en) | 2017-12-15 | 2019-06-20 | Juno Therapeutics, Inc. | Anti-cct5 binding molecules and methods of use thereof |
| CN111492068B (zh) | 2017-12-19 | 2025-03-21 | 贝克顿迪金森公司 | 与寡核苷酸相关联的颗粒 |
| CN108866174B (zh) * | 2017-12-25 | 2023-05-19 | 厦门基源医疗科技有限公司 | 一种循环肿瘤dna低频突变的检测方法 |
| CN108085341A (zh) * | 2017-12-28 | 2018-05-29 | 河南省华隆生物技术有限公司 | 一种msgv重组载体及其制备方法和应用 |
| CN112236518A (zh) * | 2018-02-23 | 2021-01-15 | 耶鲁大学 | 单细胞冻融裂解 |
| AU2019249209A1 (en) | 2018-04-05 | 2020-10-15 | Juno Therapeutics, Inc. | T cell receptors and engineered cells expressing same |
| CN112262218B (zh) | 2018-04-06 | 2024-11-08 | 10X基因组学有限公司 | 用于单细胞处理中的质量控制的系统和方法 |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| CA3097976A1 (en) | 2018-05-03 | 2019-11-07 | Becton, Dickinson And Company | High throughput multiomics sample analysis |
| CN109251961A (zh) * | 2018-06-28 | 2019-01-22 | 广西医科大学 | 一种单细胞测序检测活化t细胞的方法 |
| CN112673100B (zh) * | 2018-07-09 | 2025-04-29 | 格罗生物科学公司 | 包括非标准氨基酸的组合物及其用途 |
| KR102543325B1 (ko) | 2018-08-13 | 2023-06-13 | 루트패스 제노믹스, 인크. | 쌍을 이룬 이분 면역수용체 폴리뉴클레오티드의 고처리량 클로닝 및 이의 적용 |
| EP3837545A1 (en) | 2018-08-17 | 2021-06-23 | F. Hoffmann-La Roche AG | In vitro transcytosis assay |
| CN112912513A (zh) * | 2018-08-28 | 2021-06-04 | 贝克顿迪金森公司 | 使用糖类结合试剂和膜透过性试剂进行样品多重化 |
| ES2992135T3 (es) * | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US12466892B2 (en) | 2018-11-08 | 2025-11-11 | The Regents Of The University Of California | Systems and methods for targeting cancer cells |
| JP7618548B2 (ja) | 2018-11-08 | 2025-01-21 | ベクトン・ディキンソン・アンド・カンパニー | ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析 |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| JP7542444B2 (ja) * | 2018-12-14 | 2024-08-30 | イルミナ ケンブリッジ リミテッド | 配列決定の間のアンラベル化ヌクレオチドによるフェージング減少 |
| EP4242322B1 (en) | 2019-01-23 | 2024-08-21 | Becton, Dickinson and Company | Oligonucleotides associated with antibodies |
| CA3123303A1 (en) | 2019-01-29 | 2020-08-06 | Juno Therapeutics, Inc. | Antibodies and chimeric antigen receptors specific for receptor tyrosine kinase like orphan receptor 1 (ror1) |
| CN113454234B (zh) | 2019-02-14 | 2025-03-18 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| SG11202110956RA (en) | 2019-04-05 | 2021-10-28 | Rootpath Genomics Inc | Compositions and methods for t-cell receptor gene assembly |
| WO2020214642A1 (en) | 2019-04-19 | 2020-10-22 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN110241459A (zh) * | 2019-05-31 | 2019-09-17 | 南方医科大学南方医院 | 一种甄别样品间与独立样品自身交叉反应的免疫组库方法 |
| CN110241460A (zh) * | 2019-05-31 | 2019-09-17 | 南方医科大学南方医院 | 一种甄别独立样品自身交叉反应的免疫组库方法 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| JP2022544101A (ja) * | 2019-08-08 | 2022-10-17 | アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル | 表現型の規定されたｂ細胞及びｔ細胞のサブセットにおける、ｂ細胞とｔ細胞のトランスクリプトームの分析のためのｒｎａシークエンス法 |
| US20220315982A1 (en) * | 2019-09-04 | 2022-10-06 | Vanderbilt University | Methods for identification of antigen binding specificity of antibodies |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| EP4090764A1 (en) | 2020-01-13 | 2022-11-23 | Becton, Dickinson and Company | Cell capture using du-containing oligonucleotides |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| US20210238661A1 (en) * | 2020-01-31 | 2021-08-05 | Becton, Dickinson And Company | Mesophilic dna polymerase extension blockers |
| EP4111168A1 (en) | 2020-02-25 | 2023-01-04 | Becton Dickinson and Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| JP7727662B2 (ja) | 2020-05-13 | 2025-08-21 | ジュノー セラピューティクス インコーポレイテッド | 臨床応答に関連する特徴量の特定方法およびその使用 |
| EP4150118A1 (en) | 2020-05-14 | 2023-03-22 | Becton Dickinson and Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| CA3186974A1 (en) * | 2020-06-24 | 2021-12-30 | Claret Bioscience, Llc | Methods and compositions for analyzing nucleic acid |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| WO2022081643A2 (en) * | 2020-10-13 | 2022-04-21 | 10X Genomics, Inc. | Compositions and methods for generating recombinant antigen binding molecules from single cells |
| CN112071366B (zh) * | 2020-10-13 | 2024-02-27 | 南开大学 | 一种基于二代测序技术的宏基因组数据分析方法 |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| EP4263859A1 (en) | 2020-12-15 | 2023-10-25 | Becton, Dickinson and Company | Single cell secretome analysis |
| WO2022192603A1 (en) * | 2021-03-10 | 2022-09-15 | Factorial Diagnostics, Inc. | Cell barcoding compositions and methods |
| CN114958996B (zh) * | 2021-05-12 | 2022-12-20 | 浙江大学 | 一种超高通量单细胞测序试剂组合 |
| IL309326A (en) * | 2021-06-18 | 2024-02-01 | Pact Pharma Inc | Methods for improved sequencing of T cell receptors |
| CN115029341A (zh) * | 2022-05-23 | 2022-09-09 | 立凌生物制药(苏州)有限公司 | 一种快速克隆配对tcr序列检测方法及其应用 |
| EP4554973A1 (en) * | 2022-07-13 | 2025-05-21 | 10X Genomics, Inc. | Methods for characterization of antigen-binding molecules from biological samples |
| JP2026510460A (ja) | 2023-03-13 | 2026-04-06 | エイチ．リー モフィット キャンサー センター アンド リサーチ インスティテュート インコーポレイテッド | 選択された腫瘍浸潤リンパ球の組成物ならびに関連するその生成及び使用方法 |
| WO2025080865A1 (en) | 2023-10-11 | 2025-04-17 | Turnstone Biologics Corp. | Combination of tumor infiltrating lymphocytes (til) and low dose radiation |
| WO2025096638A2 (en) | 2023-10-30 | 2025-05-08 | Turnstone Biologics Corp. | Genetically modified tumor infilitrating lymphocytes and methods of producing and using the same |
Family Cites Families (194)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| US5242794A (en) | 1984-12-13 | 1993-09-07 | Applied Biosystems, Inc. | Detection of specific sequences in nucleic acids |
| US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4965188A (en) | 1986-08-22 | 1990-10-23 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme |
| US4766067A (en) | 1985-05-31 | 1988-08-23 | President And Fellows Of Harvard College | Gene amplification |
| GB8601597D0 (en) | 1986-01-23 | 1986-02-26 | Wilson R H | Nucleotide sequences |
| US4800159A (en) | 1986-02-07 | 1989-01-24 | Cetus Corporation | Process for amplifying, detecting, and/or cloning nucleic acid sequences |
| US4889818A (en) | 1986-08-22 | 1989-12-26 | Cetus Corporation | Purified thermostable enzyme |
| US4795699A (en) | 1987-01-14 | 1989-01-03 | President And Fellows Of Harvard College | T7 DNA polymerase |
| US4921794A (en) | 1987-01-14 | 1990-05-01 | President And Fellows Of Harvard College | T7 DNA polymerase |
| US4988617A (en) | 1988-03-25 | 1991-01-29 | California Institute Of Technology | Method of detecting a nucleotide change in nucleic acids |
| US5168038A (en) | 1988-06-17 | 1992-12-01 | The Board Of Trustees Of The Leland Stanford Junior University | In situ transcription in cells and tissues |
| US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
| US5091310A (en) | 1988-09-23 | 1992-02-25 | Cetus Corporation | Structure-independent dna amplification by the polymerase chain reaction |
| US5142033A (en) | 1988-09-23 | 1992-08-25 | Hoffmann-La Roche Inc. | Structure-independent DNA amplification by the polymerase chain reaction |
| US5066584A (en) | 1988-09-23 | 1991-11-19 | Cetus Corporation | Methods for generating single stranded dna by the polymerase chain reaction |
| US4994370A (en) | 1989-01-03 | 1991-02-19 | The United States Of America As Represented By The Department Of Health And Human Services | DNA amplification technique |
| US5242974A (en) | 1991-11-22 | 1993-09-07 | Affymax Technologies N.V. | Polymer reversal on solid surfaces |
| US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US5527681A (en) | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| DE3920358A1 (de) | 1989-06-22 | 1991-01-17 | Behringwerke Ag | Bispezifische und oligospezifische, mono- und oligovalente antikoerperkonstrukte, ihre herstellung und verwendung |
| US5252743A (en) | 1989-11-13 | 1993-10-12 | Affymax Technologies N.V. | Spatially-addressable immobilization of anti-ligands on surfaces |
| US5494810A (en) | 1990-05-03 | 1996-02-27 | Cornell Research Foundation, Inc. | Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease |
| US5210015A (en) | 1990-08-06 | 1993-05-11 | Hoffman-La Roche Inc. | Homogeneous assay system using the nuclease activity of a nucleic acid polymerase |
| AU660306B2 (en) | 1991-02-12 | 1995-06-22 | Roussel-Uclaf | Nucleotidic sequences coding for variable regions of beta chains of human T lymphocyte receptors, corresponding peptidic segments and diagnostic and therapeutic applications |
| US5384261A (en) | 1991-11-22 | 1995-01-24 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis using mechanically directed flow paths |
| US5324633A (en) | 1991-11-22 | 1994-06-28 | Affymax Technologies N.V. | Method and apparatus for measuring binding affinity |
| DE69233501T2 (de) | 1991-11-22 | 2006-02-23 | Affymetrix, Inc. (n.d.Ges.d.Staates Delaware), Santa Clara | Kombinatorische Strategien für die Polymersynthese |
| US5550215A (en) | 1991-11-22 | 1996-08-27 | Holmes; Christopher P. | Polymer reversal on solid surfaces |
| DE69232137T2 (de) | 1991-11-25 | 2002-05-29 | Enzon Inc | Multivalente antigen-bindende proteine |
| CA2124967C (en) | 1991-12-17 | 2008-04-08 | Nils Lonberg | Transgenic non-human animals capable of producing heterologous antibodies |
| US5491074A (en) | 1993-04-01 | 1996-02-13 | Affymax Technologies Nv | Association peptides |
| US5837832A (en) | 1993-06-25 | 1998-11-17 | Affymetrix, Inc. | Arrays of nucleic acid probes on biological chips |
| DE69431719T2 (de) | 1993-06-25 | 2003-09-18 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Hybridisierung und sequenzierung von nukleinsäuren |
| US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| US5925517A (en) | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| US5631734A (en) | 1994-02-10 | 1997-05-20 | Affymetrix, Inc. | Method and apparatus for detection of fluorescently labeled materials |
| US6090555A (en) | 1997-12-11 | 2000-07-18 | Affymetrix, Inc. | Scanned image alignment systems and methods |
| US5578832A (en) | 1994-09-02 | 1996-11-26 | Affymetrix, Inc. | Method and apparatus for imaging a sample on a device |
| WO1995030774A1 (en) | 1994-05-05 | 1995-11-16 | Beckman Instruments, Inc. | Oligonucleotide repeat arrays |
| US5571639A (en) | 1994-05-24 | 1996-11-05 | Affymax Technologies N.V. | Computer-aided engineering system for design of sequence arrays and lithographic masks |
| US5795716A (en) | 1994-10-21 | 1998-08-18 | Chee; Mark S. | Computer-aided visualization and analysis system for sequence evaluation |
| US5599695A (en) | 1995-02-27 | 1997-02-04 | Affymetrix, Inc. | Printing molecular library arrays using deprotection agents solely in the vapor phase |
| US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
| US5624711A (en) | 1995-04-27 | 1997-04-29 | Affymax Technologies, N.V. | Derivatization of solid supports and methods for oligomer synthesis |
| US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
| US6852487B1 (en) | 1996-02-09 | 2005-02-08 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays |
| US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| WO1997043611A1 (en) | 1996-05-16 | 1997-11-20 | Affymetrix, Inc. | Systems and methods for detection of labeled materials |
| EP1736554B1 (en) | 1996-05-29 | 2013-10-09 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| EP1033411B1 (en) | 1996-06-04 | 2006-02-22 | University of Utah Research Foundation | Fluorescent donor-acceptor pair |
| DK0937251T3 (da) | 1996-09-06 | 2007-02-19 | Ortho Mcneil Pharm Inc | Oprensning af antigenspecifikke T-celler |
| JP2002503954A (ja) | 1997-04-01 | 2002-02-05 | グラクソ、グループ、リミテッド | 核酸増幅法 |
| US6974669B2 (en) | 2000-03-28 | 2005-12-13 | Nanosphere, Inc. | Bio-barcodes based on oligonucleotide-modified nanoparticles |
| EP1027456B1 (en) | 1997-10-31 | 2005-03-16 | Affymetrix, Inc. (a Delaware Corporation) | Expression profiles in adult and fetal organs |
| US6269846B1 (en) | 1998-01-13 | 2001-08-07 | Genetic Microsystems, Inc. | Depositing fluid specimens on substrates, resulting ordered arrays, techniques for deposition of arrays |
| US6428752B1 (en) | 1998-05-14 | 2002-08-06 | Affymetrix, Inc. | Cleaning deposit devices that form microarrays and the like |
| US6054276A (en) | 1998-02-23 | 2000-04-25 | Macevicz; Stephen C. | DNA restriction site mapping |
| US5936324A (en) | 1998-03-30 | 1999-08-10 | Genetic Microsystems Inc. | Moving magnet scanner |
| AU3463699A (en) | 1998-04-03 | 1999-10-25 | Phylos, Inc. | Addressable protein arrays |
| JP3813818B2 (ja) | 1998-05-01 | 2006-08-23 | アリゾナ ボード オブ リージェンツ | オリゴヌクレオチドおよびｄｎａ分子のヌクレオチド配列の決定方法 |
| US6780591B2 (en) | 1998-05-01 | 2004-08-24 | Arizona Board Of Regents | Method of determining the nucleotide sequence of oligonucleotides and DNA molecules |
| US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| EP1165839A2 (en) | 1999-03-26 | 2002-01-02 | Whitehead Institute For Biomedical Research | Universal arrays |
| US6300070B1 (en) | 1999-06-04 | 2001-10-09 | Mosaic Technologies, Inc. | Solid phase methods for amplifying multiple nucleic acids |
| GB9928787D0 (en) | 1999-12-03 | 2000-02-02 | Medical Res Council | Direct screening method |
| US6582938B1 (en) | 2001-05-11 | 2003-06-24 | Affymetrix, Inc. | Amplification of nucleic acids |
| EP1303639A2 (en) | 2000-04-14 | 2003-04-23 | Cornell Research Foundation, Inc. | Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction |
| US6686184B1 (en) | 2000-05-25 | 2004-02-03 | President And Fellows Of Harvard College | Patterning of surfaces utilizing microfluidic stamps including three-dimensionally arrayed channel networks |
| US7567870B1 (en) | 2000-07-31 | 2009-07-28 | Institute For Systems Biology | Multiparameter analysis for predictive medicine |
| AU2002210926B2 (en) | 2000-10-24 | 2005-12-15 | Shunichi Shiozawa | Genomes participating in rheumatoid arthritis, method of the diagnosis thereof, method of evaluating the onset possibility thereof, diagnostic kit for detecting them and therapeutic method and remedies for rheumatoid arthritis |
| US7393656B2 (en) | 2001-07-10 | 2008-07-01 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for risk stratification |
| US7414111B2 (en) | 2001-09-19 | 2008-08-19 | Alexion Pharmaceuticals, Inc. | Engineered templates and their use in single primer amplification |
| GB0127564D0 (en) | 2001-11-16 | 2002-01-09 | Medical Res Council | Emulsion compositions |
| JP2006507921A (ja) | 2002-06-28 | 2006-03-09 | プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ | 流体分散のための方法および装置 |
| WO2004003820A2 (en) | 2002-07-01 | 2004-01-08 | Institut Pasteur | System, method, device, and computer program product for extraction, gathering, manipulation, and analysis of peak data from an automated sequencer |
| AU2003281336A1 (en) | 2002-07-03 | 2004-01-23 | Institute For Scientific Research, Inc. | Compositions and methods for the detection of human t cell receptor variable family gene expression |
| EP1546397A4 (en) | 2002-09-27 | 2007-10-31 | Cold Spring Harbor Lab | CELL BASED RNA INTERFERENCE AND ASSOCIATED METHODS AND COMPOSITIONS |
| DE60334471D1 (de) | 2002-10-11 | 2010-11-18 | Univ Erasmus | Primer für nukleinsäureamplifikation in pcr-basierten klonalitätsstudien |
| WO2005003375A2 (en) | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| US20060078893A1 (en) | 2004-10-12 | 2006-04-13 | Medical Research Council | Compartmentalised combinatorial chemistry by microfluidic control |
| EP2127736A1 (en) | 2003-04-10 | 2009-12-02 | The President and Fellows of Harvard College | Formation and control of fluidic species |
| AU2003902299A0 (en) | 2003-05-13 | 2003-05-29 | Flinders Medical Centre | A method of analysing a marker nucleic acid molecule |
| EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| US7767435B2 (en) | 2003-08-25 | 2010-08-03 | University Of Washington | Method and device for biochemical detection and analysis of subcellular compartments from a single cell |
| CN104069784B (zh) | 2003-08-27 | 2017-01-11 | 哈佛大学 | 流体物种的电子控制 |
| TWI333977B (en) | 2003-09-18 | 2010-12-01 | Symphogen As | Method for linking sequences of interest |
| US7169560B2 (en) | 2003-11-12 | 2007-01-30 | Helicos Biosciences Corporation | Short cycle methods for sequencing polynucleotides |
| FR2863274B1 (fr) | 2003-12-05 | 2012-11-16 | Commissariat Energie Atomique | Procede d'evaluation quantitative d'un rearrangement ou d'une recombinaison genetique ciblee d'un individu et ses applications. |
| US20080166718A1 (en) | 2003-12-15 | 2008-07-10 | Institut Pasteur | Repertoire determination of a lymphocyte B population |
| US20070160994A1 (en) | 2003-12-15 | 2007-07-12 | Institut Pasteur | Repertoire determination of a lymphocyte b population |
| US20060046258A1 (en) | 2004-02-27 | 2006-03-02 | Lapidus Stanley N | Applications of single molecule sequencing |
| WO2005084134A2 (en) * | 2004-03-04 | 2005-09-15 | Dena Leshkowitz | Quantifying and profiling antibody and t cell receptor gene expression |
| US20050221339A1 (en) | 2004-03-31 | 2005-10-06 | Medical Research Council Harvard University | Compartmentalised screening by microfluidic control |
| US7622281B2 (en) | 2004-05-20 | 2009-11-24 | The Board Of Trustees Of The Leland Stanford Junior University | Methods and compositions for clonal amplification of nucleic acid |
| US7820382B2 (en) | 2004-08-03 | 2010-10-26 | Bauer A Robert | Method for the early detection of breast cancer, lung cancer, pancreatic cancer and colon polyps, growths and cancers as well as other gastrointestinal disease conditions and the preoperative and postoperative monitoring of transplanted organs from the donor and in the recipient and their associated conditions related and unrelated to the organ transplantation |
| US20060094018A1 (en) | 2004-08-03 | 2006-05-04 | Bauer A R Jr | Discovery and a method for the early detection of pancreatic cancer and other disease conditions |
| US20080280282A1 (en) | 2004-08-03 | 2008-11-13 | Bauer Jr A Robert | Method for early detection of various cancers and gastrointestinal disease and monitoring of transplanted organs |
| US7915036B2 (en) | 2004-09-13 | 2011-03-29 | The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Compositions comprising T cell receptors and methods of use thereof |
| US7643818B2 (en) | 2004-11-22 | 2010-01-05 | Seven Networks, Inc. | E-mail messaging to/from a mobile terminal |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| EP2248578B1 (en) | 2005-03-04 | 2012-06-06 | President and Fellows of Harvard College | Method for forming multiple emulsions |
| US8592136B2 (en) | 2005-09-13 | 2013-11-26 | Affymetrix, Inc. | Methods for producing codes for microparticles |
| EP2428579B1 (en) | 2005-10-24 | 2013-05-29 | The Johns Hopkins University | Improved methods for BEAMing |
| US7375211B2 (en) | 2005-11-18 | 2008-05-20 | Kou Zhong C | Method for detection and quantification of T-cell receptor Vβ repertoire |
| US20070161031A1 (en) | 2005-12-16 | 2007-07-12 | The Board Of Trustees Of The Leland Stanford Junior University | Functional arrays for high throughput characterization of gene expression regulatory elements |
| JP2009536313A (ja) | 2006-01-11 | 2009-10-08 | レインダンス テクノロジーズ， インコーポレイテッド | ナノリアクターの形成および制御において使用するマイクロ流体デバイスおよび方法 |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| WO2007089541A2 (en) | 2006-01-27 | 2007-08-09 | President And Fellows Of Harvard College | Fluidic droplet coalescence |
| JP2009528828A (ja) | 2006-03-06 | 2009-08-13 | シムフォゲン・アクティーゼルスカブ | 消化器多核体ウイルス感染症の治療のための組み換えポリクローナル抗体 |
| ATE540750T1 (de) | 2006-05-11 | 2012-01-15 | Raindance Technologies Inc | Mikrofluidische vorrichtung und verfahren |
| CN101506378A (zh) | 2006-06-19 | 2009-08-12 | 约翰·霍普金斯大学 | 在油包水乳液中的微粒上的单分子pcr |
| US20080108804A1 (en) | 2006-11-02 | 2008-05-08 | Kabushiki Kaisha Dnaform | Method for modifying RNAS and preparing DNAS from RNAS |
| US20100094795A1 (en) | 2006-11-30 | 2010-04-15 | Johns Hopkins University | Gene expression barcode for normal and diseased tissue classification |
| WO2008076842A2 (en) | 2006-12-14 | 2008-06-26 | Applied Biosystems Inc. | Sequencing methods |
| US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
| CN102083575B (zh) | 2007-06-25 | 2014-09-17 | 阿费梅特里克斯公司 | 刻印的微码 |
| EP2173772A2 (en) | 2007-07-03 | 2010-04-14 | Ablynx N.V. | Providing improved immunoglobulin sequences by mutating cdr and/or fr positions |
| PL2036989T3 (pl) | 2007-09-12 | 2012-12-31 | Pasteur Institut | Polinukleotyd odpowiedni do testu reporterowego dla pojedynczych komórek do monitorowania schematów ekspresji genów z dużą rozdzielczością przestrzenną i czasową |
| US8268564B2 (en) | 2007-09-26 | 2012-09-18 | President And Fellows Of Harvard College | Methods and applications for stitched DNA barcodes |
| US20100285984A1 (en) | 2007-09-28 | 2010-11-11 | Wettstein Peter J | Assessing t cell repertoires |
| EP2062982A1 (fr) | 2007-11-26 | 2009-05-27 | ImmunID | Procédé d'étude de la diversité combinatoire V(D)J |
| WO2009076485A2 (en) | 2007-12-10 | 2009-06-18 | Xiaolian Gao | Sequencing of nucleic acids |
| US20090163366A1 (en) | 2007-12-24 | 2009-06-25 | Helicos Biosciences Corporation | Two-primer sequencing for high-throughput expression analysis |
| EP2100970B1 (en) | 2008-03-13 | 2017-05-10 | National Institute of Immunology | Ig genes specific oligonucleotides and uses thereof |
| US9012148B2 (en) | 2008-04-16 | 2015-04-21 | Jian Han | Method for evaluating and comparing immunorepertoires |
| DK2279263T3 (da) | 2008-04-30 | 2013-11-11 | Integrated Dna Tech Inc | Rnase-h-baserede assays med anvendelse af modificerede rna-monomerer |
| US8911948B2 (en) | 2008-04-30 | 2014-12-16 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| US8198028B2 (en) | 2008-07-02 | 2012-06-12 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
| EP2315629B1 (en) | 2008-07-18 | 2021-12-15 | Bio-Rad Laboratories, Inc. | Droplet libraries |
| US20100062494A1 (en) | 2008-08-08 | 2010-03-11 | President And Fellows Of Harvard College | Enzymatic oligonucleotide pre-adenylation |
| WO2010021936A1 (en) | 2008-08-16 | 2010-02-25 | The Board Of Trustees Of The Leland Stanford Junior University | Digital pcr calibration for high throughput sequencing |
| US9115399B2 (en) | 2008-09-23 | 2015-08-25 | The Trustees Of The University Of Pennsylvania | Recombination sequence (RS) rearrangement frequency as a measure of central B cell tolerance |
| CA2738578C (en) | 2008-09-23 | 2021-05-04 | Quantalife, Inc. | Droplet-based assay system |
| RU2011117213A (ru) | 2008-09-30 | 2012-11-10 | Эббот Лэборетриз (Us) | Улучшенные библиотеки антител |
| EP2359139B1 (en) | 2008-11-07 | 2018-03-07 | National Jewish Health | Diagnosis and treatment of autommune diseases by targeting autommune-related b cells ("abcs") |
| US8748103B2 (en) | 2008-11-07 | 2014-06-10 | Sequenta, Inc. | Monitoring health and disease status using clonotype profiles |
| CN104195227B (zh) | 2008-11-07 | 2017-04-12 | 适应生物技术公司 | 通过序列分析监测状况的方法 |
| US8691510B2 (en) | 2008-11-07 | 2014-04-08 | Sequenta, Inc. | Sequence analysis of complex amplicons |
| EP2387627B1 (en) | 2009-01-15 | 2016-03-30 | Adaptive Biotechnologies Corporation | Adaptive immunity profiling and methods for generation of monoclonal antibodies |
| AU2010206843B2 (en) | 2009-01-20 | 2015-01-29 | The Board Of Trustees Of The Leland Stanford Junior University | Single cell gene expression for diagnosis, prognosis and identification of drug targets |
| US9079942B2 (en) | 2009-02-09 | 2015-07-14 | Epitomics, Inc. | CDR-anchored amplification method |
| GB0905023D0 (en) | 2009-03-24 | 2009-05-06 | Univ Erasmus Medical Ct | Binding molecules |
| DK3002337T3 (en) * | 2009-03-30 | 2019-02-18 | Illumina Inc | ANALYSIS OF EXPRESSION OF GENES IN SINGLE CELLS |
| US20120266260A1 (en) | 2009-05-26 | 2012-10-18 | Hitoshi Suzuki | Diagnosing and treating iga nephropathy |
| CA2763199A1 (en) | 2009-05-29 | 2010-12-02 | The Regents Of The Univeristy Of California | B cell signature associated with tolerance in transplant recipients |
| CN102459643B (zh) | 2009-06-25 | 2016-06-01 | 弗雷德哈钦森癌症研究中心 | 检测获得性免疫的方法 |
| US8497071B2 (en) | 2009-06-29 | 2013-07-30 | California Institute Of Technology | Isolation of unknown rearranged T-cell receptors from single cells |
| GB2471473A (en) | 2009-06-30 | 2011-01-05 | Owen Mumford Ltd | Syringe sheath remover |
| CA2772298A1 (en) | 2009-08-26 | 2011-03-03 | Research Development Foundation | Methods for creating antibody libraries |
| US8293483B2 (en) | 2009-09-11 | 2012-10-23 | Epitomics, Inc. | Method for identifying lineage-related antibodies |
| US20110086051A1 (en) | 2009-10-08 | 2011-04-14 | Dartmouth-Hitchcock Clinic | System and method for monitoring and optimizing immune status in transplant recipients |
| GB0918564D0 (en) | 2009-10-22 | 2009-12-09 | Plasticell Ltd | Nested cell encapsulation |
| WO2011049603A1 (en) | 2009-10-22 | 2011-04-28 | Dana-Farber Cancer Institute, Inc. | Biomarkers to identify hiv-specific t-cell subsets |
| HUE052213T2 (hu) | 2009-11-06 | 2021-04-28 | Univ Leland Stanford Junior | Grafitkilökõdés nem invazív diagnosztizálása szervátültetett betegekben |
| US9399797B2 (en) | 2010-02-12 | 2016-07-26 | Raindance Technologies, Inc. | Digital analyte analysis |
| CA2789425C (en) | 2010-02-12 | 2020-04-28 | Raindance Technologies, Inc. | Digital analyte analysis with polymerase error correction |
| WO2011106558A1 (en) | 2010-02-24 | 2011-09-01 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for autoimmune disease diagnosis, prognosis, and treatment |
| WO2011119980A1 (en) | 2010-03-25 | 2011-09-29 | The Board Of Trustees Of The Leland Stanford Junior University | Protein and gene biomarkers for rejection of organ transplants |
| EP4219759A3 (en) | 2010-05-06 | 2023-09-06 | Adaptive Biotechnologies Corporation | Monitoring health and disease status using clonotype profiles |
| WO2011140433A2 (en) | 2010-05-07 | 2011-11-10 | The Board Of Trustees Of The Leland Stanford Junior University | Measurement and comparison of immune diversity by high-throughput sequencing |
| CN103003697A (zh) | 2010-05-17 | 2013-03-27 | 得克萨斯系统大学董事会 | 来自动物的单克隆抗体的快速分离 |
| US9650629B2 (en) | 2010-07-07 | 2017-05-16 | Roche Molecular Systems, Inc. | Clonal pre-amplification in emulsion |
| WO2012027503A2 (en) | 2010-08-24 | 2012-03-01 | Fred Hutchinson Cancer Research Center | Method of measuring adaptive immunity |
| CA2811185C (en) | 2010-09-21 | 2020-09-22 | Population Genetics Technologies Ltd. | Increasing confidence of allele calls with molecular counting |
| WO2012042374A2 (en) | 2010-10-01 | 2012-04-05 | Anssi Jussi Nikolai Taipale | Method of determining number or concentration of molecules |
| EP2625320B1 (en) * | 2010-10-08 | 2019-03-27 | President and Fellows of Harvard College | High-throughput single cell barcoding |
| EP2625295B1 (en) | 2010-10-08 | 2019-03-13 | President and Fellows of Harvard College | High-throughput immune sequencing |
| EP2646573A1 (en) | 2010-12-01 | 2013-10-09 | MorphoSys AG | Simultaneous detection of biomolecules in single cells |
| WO2012083069A2 (en) | 2010-12-15 | 2012-06-21 | The Board Of Trustees Of The Leland Stanford Junior University | Measurement and monitoring of cell clonality |
| WO2012083225A2 (en) * | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| CN103620405B (zh) | 2010-12-31 | 2016-05-25 | 生物蛋白有限公司 | 全面单克隆抗体产生 |
| WO2012097374A1 (en) | 2011-01-14 | 2012-07-19 | Cb Biotechnologies, Inc. | Immunodiversity assessment method and its use |
| EP3736281A1 (en) * | 2011-02-18 | 2020-11-11 | Bio-Rad Laboratories, Inc. | Compositions and methods for molecular labeling |
| ES2625288T3 (es) | 2011-04-15 | 2017-07-19 | The Johns Hopkins University | Sistema de secuenciación segura |
| US9347059B2 (en) * | 2011-04-25 | 2016-05-24 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| PT3415619T (pt) * | 2011-04-28 | 2021-03-22 | Univ Leland Stanford Junior | Identificação de polinucleótidos associados a uma amostra |
| EP3709018A1 (en) | 2011-06-02 | 2020-09-16 | Bio-Rad Laboratories, Inc. | Microfluidic apparatus for identifying components of a chemical reaction |
| JP6731702B2 (ja) | 2011-07-12 | 2020-07-29 | エックスバイオテク, インコーポレイテッドXbiotech, Inc. | 親和性成熟化ヒト抗体の同定 |
| GB2496016B (en) | 2011-09-09 | 2016-03-16 | Univ Leland Stanford Junior | Methods for obtaining a sequence |
| EP2758550B1 (en) | 2011-09-22 | 2016-10-26 | Lineage Biosciences, Inc. | Detection of isotype profiles as signatures for disease |
| EP2817418B1 (en) * | 2012-02-24 | 2017-10-11 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
| WO2013134261A1 (en) * | 2012-03-05 | 2013-09-12 | President And Fellows Of Harvard College | Systems and methods for epigenetic sequencing |
| WO2014071361A1 (en) * | 2012-11-05 | 2014-05-08 | Rubicon Genomics | Barcoding nucleic acids |
| US10119134B2 (en) | 2013-03-15 | 2018-11-06 | Abvitro Llc | Single cell bar-coding for antibody discovery |
| US20160122753A1 (en) * | 2013-06-12 | 2016-05-05 | Tarjei Mikkelsen | High-throughput rna-seq |
| TWI707038B (zh) * | 2013-08-05 | 2020-10-11 | 美商扭轉生物科技有限公司 | 重新合成之基因庫 |
| CA2935122C (en) * | 2013-12-30 | 2023-09-19 | Atreca, Inc. | Analysis of nucleic acids associated with single cells using nucleic acid barcodes |
| ES2819277T3 (es) * | 2014-02-11 | 2021-04-15 | Hoffmann La Roche | Secuenciación dirigida y filtrado de UID |
| EP3110971B1 (en) * | 2014-02-27 | 2019-05-08 | The Broad Institute Inc. | T cell balance gene expression and methods of use thereof |
| ES2895750T3 (es) | 2014-09-15 | 2022-02-22 | Abvitro Llc | Secuenciación de alto rendimiento de colecciones de nucleótidos |
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