JP2020519304A5 - - Google Patents
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- Publication number
- JP2020519304A5 JP2020519304A5 JP2020500937A JP2020500937A JP2020519304A5 JP 2020519304 A5 JP2020519304 A5 JP 2020519304A5 JP 2020500937 A JP2020500937 A JP 2020500937A JP 2020500937 A JP2020500937 A JP 2020500937A JP 2020519304 A5 JP2020519304 A5 JP 2020519304A5
- Authority
- JP
- Japan
- Prior art keywords
- exonuclease
- nucleic acid
- acid molecule
- target nucleic
- rect
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 101700008821 EXO Proteins 0.000 claims 35
- 101700083023 EXRN Proteins 0.000 claims 34
- 108020004707 nucleic acids Proteins 0.000 claims 33
- 150000007523 nucleic acids Chemical class 0.000 claims 33
- 210000004027 cells Anatomy 0.000 claims 20
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 19
- 238000000137 annealing Methods 0.000 claims 12
- 102000004169 proteins and genes Human genes 0.000 claims 10
- 108090000623 proteins and genes Proteins 0.000 claims 10
- 238000010367 cloning Methods 0.000 claims 8
- 101700011961 DPOM Proteins 0.000 claims 7
- 101710029649 MDV043 Proteins 0.000 claims 7
- 101700061424 POLB Proteins 0.000 claims 7
- 101700054624 RF1 Proteins 0.000 claims 7
- 241000588724 Escherichia coli Species 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 6
- 238000002744 homologous recombination Methods 0.000 claims 5
- 210000004436 Chromosomes, Artificial, Bacterial Anatomy 0.000 claims 4
- 108010017826 DNA Polymerase I Proteins 0.000 claims 4
- 102000004594 DNA Polymerase I Human genes 0.000 claims 4
- 102000013165 Exonucleases Human genes 0.000 claims 4
- 108060002716 Exonucleases Proteins 0.000 claims 4
- 102000001218 Rec A Recombinases Human genes 0.000 claims 4
- 108010055016 Rec A Recombinases Proteins 0.000 claims 4
- 230000029087 digestion Effects 0.000 claims 4
- 230000002255 enzymatic Effects 0.000 claims 4
- 238000000338 in vitro Methods 0.000 claims 4
- 229920002676 Complementary DNA Polymers 0.000 claims 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims 3
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 3
- 108060009497 WRNexo Proteins 0.000 claims 3
- 230000001580 bacterial Effects 0.000 claims 3
- 239000002299 complementary DNA Substances 0.000 claims 3
- 230000000694 effects Effects 0.000 claims 3
- 239000002773 nucleotide Substances 0.000 claims 3
- 125000003729 nucleotide group Chemical group 0.000 claims 3
- 108060003435 Beta Proteins 0.000 claims 2
- 210000000349 Chromosomes Anatomy 0.000 claims 2
- 108010042407 Endonucleases Proteins 0.000 claims 2
- 102000004533 Endonucleases Human genes 0.000 claims 2
- 235000003534 Saccharomyces carlsbergensis Nutrition 0.000 claims 2
- 229940081969 Saccharomyces cerevisiae Drugs 0.000 claims 2
- 238000003259 recombinant expression Methods 0.000 claims 2
- 101710008158 D15 Proteins 0.000 claims 1
- 241000588722 Escherichia Species 0.000 claims 1
- 101710027747 MT2151 Proteins 0.000 claims 1
- 108050002092 RAD52 Proteins 0.000 claims 1
- 102100002821 RAD52 Human genes 0.000 claims 1
- 108010068097 Rad51 Recombinase Proteins 0.000 claims 1
- 102000002490 Rad51 Recombinase Human genes 0.000 claims 1
- 229940047431 Recombinate Drugs 0.000 claims 1
- 230000015572 biosynthetic process Effects 0.000 claims 1
- 238000003776 cleavage reaction Methods 0.000 claims 1
- 238000010276 construction Methods 0.000 claims 1
- 210000004962 mammalian cells Anatomy 0.000 claims 1
- 238000005215 recombination Methods 0.000 claims 1
- 108091007521 restriction endonucleases Proteins 0.000 claims 1
- 238000003786 synthesis reaction Methods 0.000 claims 1
- 230000002194 synthesizing Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710177676.1A CN106995813B (zh) | 2017-03-23 | 2017-03-23 | 基因组大片段直接克隆和dna多分子组装新技术 |
| CN201710177676.1 | 2017-03-23 | ||
| PCT/CN2017/000483 WO2018170614A1 (zh) | 2017-03-23 | 2017-08-02 | 基因组大片段直接克隆和dna多分子组装新技术 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020519304A JP2020519304A (ja) | 2020-07-02 |
| JP2020519304A5 true JP2020519304A5 (es) | 2021-09-24 |
| JP7106625B2 JP7106625B2 (ja) | 2022-07-26 |
Family
ID=59431502
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020500937A Active JP7106625B2 (ja) | 2017-03-23 | 2017-08-02 | ゲノム大断片のダイレクトクローニングおよびｄｎａマルチ分子構築の新手法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US20200010867A1 (es) |
| EP (1) | EP3604524B1 (es) |
| JP (1) | JP7106625B2 (es) |
| KR (1) | KR102488128B1 (es) |
| CN (1) | CN106995813B (es) |
| CA (1) | CA3060585A1 (es) |
| DK (1) | DK3604524T3 (es) |
| ES (1) | ES2902210T3 (es) |
| WO (1) | WO2018170614A1 (es) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
| US11293019B2 (en) | 2017-12-22 | 2022-04-05 | Gflas Life Sciences, Inc. | Chimeric genome engineering molecules and methods |
| CN108676847A (zh) * | 2017-12-30 | 2018-10-19 | 深圳人体密码基因科技有限公司 | 大片段基因组的捕获探针的制备方法及试剂盒 |
| CN111088275B (zh) * | 2018-10-23 | 2023-06-27 | 黄菁 | Dna大片段的克隆方法 |
| JP2022524044A (ja) * | 2019-03-08 | 2022-04-27 | ザイマージェン インコーポレイテッド | 微生物のプール型ゲノム編集 |
| CN110846239B (zh) * | 2019-11-29 | 2022-08-23 | 南京工业大学 | 具有高同源重组效率的重组解脂耶氏酵母菌及其构建方法和应用 |
| CN112852849B (zh) * | 2019-12-31 | 2023-03-14 | 湖北伯远合成生物科技有限公司 | 一种用于大片段dna无缝组装的系统及组装方法 |
| WO2021163637A1 (en) * | 2020-02-13 | 2021-08-19 | Zymergen Inc. | Metagenomic library and natural product discovery platform |
| CN113355339B (zh) * | 2020-03-05 | 2023-01-24 | 山东大学 | 一种大型基因簇的无痕定点改造方法及其应用 |
| CN111440753A (zh) * | 2020-03-16 | 2020-07-24 | 扬州大学 | 禽致病性大肠杆菌clbH基因缺失株及其构建方法和应用 |
| WO2023178544A1 (zh) * | 2022-03-23 | 2023-09-28 | 深圳华大生命科学研究院 | 表达元件组合、表达载体、宿主、应用及组装方法 |
| CN115960733B (zh) * | 2022-09-19 | 2023-11-24 | 苏州泓迅生物科技股份有限公司 | 一种用于大片段dna组装的基因工程酵母菌、构建方法及其应用 |
| CN116355931A (zh) * | 2023-05-29 | 2023-06-30 | 北京因诺惟康医药科技有限公司 | 一种快速分子克隆方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL120338A0 (en) * | 1997-02-27 | 1997-06-10 | Gesher Israel Advanced Biotecs | Single step DNA fragments assembly |
| US20040101520A1 (en) | 2001-02-09 | 2004-05-27 | Muijrers Joep P P | Recombination method |
| US7723077B2 (en) | 2005-08-11 | 2010-05-25 | Synthetic Genomics, Inc. | In vitro recombination method |
| EP1929012B1 (en) | 2005-08-11 | 2010-10-06 | Synthetic Genomics, Inc. | Method for in vitro recombination |
| GB201009732D0 (en) * | 2010-06-10 | 2010-07-21 | Gene Bridges Gmbh | Direct cloning |
| CN102634534A (zh) | 2012-03-30 | 2012-08-15 | 深圳市中联生物科技开发有限公司 | 基于同源重组的核酸分子克隆方法及相关试剂盒 |
| EP3778891A1 (en) | 2014-08-27 | 2021-02-17 | New England Biolabs, Inc. | Synthon formation |
| CN106995813B (zh) * | 2017-03-23 | 2020-06-16 | 山东大学 | 基因组大片段直接克隆和dna多分子组装新技术 |
-
2017
- 2017-03-23 CN CN201710177676.1A patent/CN106995813B/zh active Active
- 2017-08-02 ES ES17901979T patent/ES2902210T3/es active Active
- 2017-08-02 KR KR1020197031095A patent/KR102488128B1/ko active IP Right Grant
- 2017-08-02 CA CA3060585A patent/CA3060585A1/en active Pending
- 2017-08-02 WO PCT/CN2017/000483 patent/WO2018170614A1/zh unknown
- 2017-08-02 JP JP2020500937A patent/JP7106625B2/ja active Active
- 2017-08-02 DK DK17901979.9T patent/DK3604524T3/da active
- 2017-08-02 EP EP17901979.9A patent/EP3604524B1/en active Active
-
2019
- 2019-09-22 US US16/578,385 patent/US20200010867A1/en not_active Abandoned
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