JP2020519230A - がんの治療のための、補体不活性化に耐性のエンベロープウイルス - Google Patents
がんの治療のための、補体不活性化に耐性のエンベロープウイルス Download PDFInfo
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Abstract
Description
Annex C/ST.25テキストファイルの形式で電子出願された配列表及び関連ファイル参照21003−PCTは、本開示の一部である。
[実施例]
Carroll, M. C., E. M. Alicot, P. J. Katzman, L. B. Klickstein, J. A. Smith, and D. T. Fearon. 1988. Organization of the genes encoding complement receptors type 1 and 2, decay-accelerating factor, and C4-binding protein in the RCA locus on human chromosome 1. J. Exp. Med. 167:1271
Rey-Campos, J., P. Rubinstein, and S. Rodriguez de Cordoba. 1988. A physical map of the human regulator of complement activation gene cluster linking the complement genes CR1, CR2, DAF, and C4BP. J. Exp. Med. 167:664
Lublin, D. M., and J. P. Atkinson. 1989. Decay-accelerating factor: biochemistry, molecular biology, and function. Annu. Rev. Immunol. 7:35. 5. Nakano, Y., K. Sumida, N. Kikuta, N. H. Miura, T. Tobe, and M. Tomita. 1992. Complete determination of disulfide bonds localized within the short consensus repeat units of decay accelerating factor (CD55 antigen). Biochim. Biophys. Acta 1116:235
Censullo, P., and M.A. Davitz. 1994a. How GPI-anchored proteins turnover: or where do they go after arrival at the plasma membrane. Semin Immunol. 6:81
Censullo, P., and M.A. Davitz. 1994b. The fate of GPI-anchored molecules. Braz J. Med. Biol. Res. 27:289
Morgan, B. P., and S. Meri. 1994. Membrane proteins that protect against complement lysis. Springer Semin. Immunopathol. 15:369
Turner A.J. 1994. PIG-tailed membrane proteins. Essays Biochem. 28:113
Kim D.D., and W.C. Song. 2006. Membrane complement regulatory proteins. Clin. Immunol. 118:127
Pecora, A.L., Rizvi, N., Cohen, G.I., Meropol, N.J., Sterman, D., Marshall, J.L., Goldberg, S., Gross, P., O’Neil, J.D., Groene, W.S., Roberts, M.S., Rabin, H., Bamat, M.K., and R.M. Lorence. 2002. Phase I trial of intravenous administration of PV701, an oncolytic virus, in patients with advanced solid cancers. J. Clin. Oncol. 20:2251
Laurie, S.A., Bell, J.C., Atkins, H.L., Roach, J., Bamat, M.K., O’Neil, J.D., Roberts, M.S., Groene, W.S., and R.M. Lorence. 2006. A phase 1 clinical study of intravenous administration of PV701, an oncolytic virus, using two-step desensitization. Clin. Cancer Res. 12:2555
Lorence, R.M., Roberts, M.S., O’Neil, J.D., Groene, W.S., Miller, J.A., Mueller, S.N., and M.K. Bamat. 2007. Phase 1 clinical experience using intravenous administration of PV701, an oncolytic Newcastle disease virus. 7:157
Biswas, M., Johnson, J.B., Kumar, S.R.P. Parks, G.D., and E. Subbiah. 2012. Incorporation of host complement regulatory proteins into Newcastle disease virus enhances complement evasion. J. Virol. 86:12708
Rangaswamy, U.S., Cotter, C.R., Chang, X., Jin, H., and Z. Chen. 2016. CD55 is a key complement regulatory protein that counteracts complement-mediated inactivation of Newcastle disease virus. J. Gen. Virol. 97:1765
Claims (34)
- (a)CD55ペプチド配列、(b)前記CD55配列のC末端側にリンカー配列、(c)前記リンカー配列のC末端側に膜貫通ドメイン、及び(d)前記膜貫通ドメインのC末端側に細胞内ドメインを含む融合タンパク質であって、GPIアンカーを含有しない、前記融合タンパク質。
- CD55ペプチド配列がヒトCD55ペプチド配列である、請求項1に記載のタンパク質。
- 配列(a)がCD55の4個のショートコンセンサスリピート(SCR)を含む、請求項1に記載のタンパク質。
- リンカーが(G4S1)3リンカーである、請求項1に記載のタンパク質。
- 膜貫通ドメインがCD8膜貫通ドメインである、請求項1に記載のタンパク質。
- 細胞内ドメインが、トランケートしたCD8細胞内ドメインである、請求項1に記載のタンパク質。
- ペプチド配列(a)が、単一のペプチド結合によってペプチド配列(b)と共有結合している、請求項1に記載のタンパク質。
- ペプチド配列(a)が、スペーサーによってペプチド配列(b)と共有結合している、請求項1に記載のタンパク質。
- ペプチド配列(b)が、単一のペプチド結合によってペプチド配列(c)と共有結合している、請求項1に記載のタンパク質。
- ペプチド配列(b)が、スペーサーによってペプチド配列(c)と共有結合している、請求項1に記載のタンパク質。
- ペプチド配列(c)が、単一のペプチド結合によってペプチド配列(d)と共有結合している、請求項1に記載のタンパク質。
- ペプチド配列(c)が、スペーサーによってペプチド配列(d)と共有結合している、請求項1に記載のタンパク質。
- 融合タンパク質が、配列(a)のN末端側に分泌シグナルペプチドをさらに含む、請求項1に記載のタンパク質。
- 分泌シグナルペプチドがCD55の分泌シグナルペプチドである、請求項13に記載のタンパク質。
- N末端分泌シグナルペプチドが、単一のペプチドによって配列(a)と共有結合している、請求項13に記載のタンパク質。
- N末端分泌シグナルペプチドが、スペーサーによって配列(a)と共有結合している、請求項13に記載のタンパク質。
- 配列番号2の配列を有する、請求項1に記載のタンパク質。
- 配列番号3の配列を有する、請求項1に記載のタンパク質。
- 請求項1〜18のいずれかに記載のタンパク質をコードする核酸。
- DNAである、請求項19に記載の核酸。
- 1又は2以上のイントロンをさらに含む、請求項20に記載の核酸。
- 配列番号2の配列を有するタンパク質をコードする、請求項19に記載の核酸。
- 配列番号1の配列を有する、請求項22に記載の核酸。
- 制御配列に作動可能に連結された、請求項19〜23のいずれかに記載の核酸を含む発現ベクター。
- 請求項1〜18のいずれかに記載のタンパク質を細胞表面に安定に発現する細胞株。
- 哺乳動物細胞株である、請求項25に記載の細胞株。
- DF−1ニワトリ胚線維芽細胞株である、請求項25に記載の細胞株。
- 請求項1〜18のいずれかに記載のタンパク質をウイルス膜上に組み込んでいるエンベロープウイルス。
- 腫瘍溶解性ウイルスである、請求項28に記載のウイルス。
- 腫瘍溶解性ウイルスがニューカッスル病ウイルスである、請求項29に記載のウイルス。
- 請求項29又は30に記載のウイルスと、薬学的に許容される担体とを含む医薬組成物。
- 哺乳動物対象において新生物状態を治療するための方法であって、前記状態を治療するのに有効な量の、請求項28に記載のウイルスを、前記対象に投与することを含む、前記方法。
- ウイルスが腫瘍内投与される、請求項32に記載の方法。
- ウイルスが静脈内投与される、請求項32に記載の方法。
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US10946068B2 (en) | 2017-12-06 | 2021-03-16 | Pandion Operations, Inc. | IL-2 muteins and uses thereof |
US10174092B1 (en) | 2017-12-06 | 2019-01-08 | Pandion Therapeutics, Inc. | IL-2 muteins |
US11981715B2 (en) | 2020-02-21 | 2024-05-14 | Pandion Operations, Inc. | Tissue targeted immunotolerance with a CD39 effector |
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Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10313865A (ja) * | 1997-05-15 | 1998-12-02 | Deinabetsuku Kenkyusho:Kk | ヒト補体制御因子が呈示されたベクター |
JP2002509695A (ja) * | 1997-11-21 | 2002-04-02 | ゲセルシャフト フュア バイオテクノロギッシェフォーシュング エムベーハー(ゲーベーエフ) | 一本鎖dna又は二本鎖dna、コード化融合タンパク質,ウイルス,パッケージング細胞及びこれらの使用ヒト補体システムによる不活化に耐えるウイルスの開発 |
JP2008507272A (ja) * | 2004-07-21 | 2008-03-13 | ダナ−ファーバー キャンサー インスティチュート,インコーポレイテッド | レンチウイルスベクターおよびその使用 |
JP2008061539A (ja) * | 2006-09-05 | 2008-03-21 | Osaka Univ | ヒト補体制御因子発現遺伝子およびその利用 |
WO2016174407A1 (en) * | 2015-04-27 | 2016-11-03 | Ucl Business Plc | Nucleic acid construct for expressing more than one chimeric antigen receptor |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5374548A (en) | 1986-05-02 | 1994-12-20 | Genentech, Inc. | Methods and compositions for the attachment of proteins to liposomes using a glycophospholipid anchor |
DE69435118D1 (de) | 1993-04-30 | 2008-08-28 | Wellstat Biologics Corp | Gereinigte Zusammensetzungen von Newcastle-Krankheitvirus |
BR9814370A (pt) | 1997-12-22 | 2000-10-10 | Univ Tennessee Research Corp A | Rhabdovirus recombinante contendo uma proteìna de fusão heteróloga. |
NZ507063A (en) * | 1998-04-02 | 2003-11-28 | Rigel Pharmaceuticals Inc | Self-dimerising peptides causing the formation of compact structures |
EP1390046A4 (en) | 1999-04-15 | 2005-04-20 | Wellstat Biologics Corp | TREATMENT OF NEOPLASMS WITH VIRUSES |
JP4915980B2 (ja) | 2002-11-15 | 2012-04-11 | エムユーエスシー ファウンデーション フォー リサーチ デベロップメント | 補体レセプター2標的化補体調節因子 |
US20100120628A1 (en) * | 2007-02-06 | 2010-05-13 | Genizon Biosciences Inc. | Genemap of the human genes associated with adhd |
KR20090122465A (ko) | 2007-03-01 | 2009-11-30 | 어드벤스드 비젼 테라피스, 인코포레이티드 | 염증성 질환의 치료 |
US8877896B2 (en) | 2008-02-15 | 2014-11-04 | Tufts University | Compositions, methods and kits for modeling, diagnosing, and treating complement disorders |
BR112013027635A2 (pt) | 2011-04-29 | 2019-09-24 | Keygene Nv | sequência de nucleotídeos, enzima, vetor, hospedeiro, planta ou planta transgênica ou parte da mesma, semente, métodos para fornecer uma planta (célula) com resistência a glifosato, para acentuar resistência a glifosato de uma planta (célula), e para gerar um produto de planta, produto de planta, usos de uma sequência de nucleotídeos e de uma mutação |
MX350445B (es) | 2011-05-05 | 2017-09-07 | Wellstat Immunotherapeutics Llc | Analogos del factor b del complemento y sus usos. |
EP2785744B1 (en) * | 2011-12-01 | 2017-10-04 | AP Biosciences, Inc. | Protein inhibitors to complement and vegf pathways and methods of use thereof |
CA2948462A1 (en) | 2014-05-15 | 2015-11-19 | National University Of Singapore | Modified natural killer cells and uses thereof |
KR20180012747A (ko) * | 2015-04-06 | 2018-02-06 | 서브도메인, 엘엘씨 | 드 노보 결합 도메인 함유 폴리펩티드 및 그의 용도 |
JP2018522833A (ja) | 2015-06-12 | 2018-08-16 | イミューノメディクス、インコーポレイテッドImmunomedics, Inc. | キメラ抗原受容体(car)コンストラクト、及びcarコンストラクトを発現するt細胞(car−t)またはnk細胞(car−nk)による疾患治療 |
EP3458576A4 (en) | 2016-05-19 | 2020-03-25 | Turnstone Limited Partnership | PSEUDOTYPED ONCOLYTIC RHABDOVIRUSES AND THEIR USE IN POLYTHERAPY |
-
2018
- 2018-05-10 WO PCT/US2018/032018 patent/WO2018209052A1/en unknown
- 2018-05-10 IL IL263979A patent/IL263979B2/en unknown
- 2018-05-10 EP EP18798575.9A patent/EP3621635A4/en active Pending
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- 2018-05-10 JP JP2018564966A patent/JP7161940B2/ja active Active
- 2018-05-10 MX MX2018015599A patent/MX2018015599A/es unknown
- 2018-05-10 CN CN201880002553.7A patent/CN109414483A/zh active Pending
- 2018-05-10 AU AU2018265258A patent/AU2018265258B2/en active Active
- 2018-11-28 ZA ZA2018/08040A patent/ZA201808040B/en unknown
-
2022
- 2022-02-18 US US17/674,990 patent/US20220169701A1/en not_active Abandoned
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH10313865A (ja) * | 1997-05-15 | 1998-12-02 | Deinabetsuku Kenkyusho:Kk | ヒト補体制御因子が呈示されたベクター |
JP2002509695A (ja) * | 1997-11-21 | 2002-04-02 | ゲセルシャフト フュア バイオテクノロギッシェフォーシュング エムベーハー(ゲーベーエフ) | 一本鎖dna又は二本鎖dna、コード化融合タンパク質,ウイルス,パッケージング細胞及びこれらの使用ヒト補体システムによる不活化に耐えるウイルスの開発 |
JP2008507272A (ja) * | 2004-07-21 | 2008-03-13 | ダナ−ファーバー キャンサー インスティチュート,インコーポレイテッド | レンチウイルスベクターおよびその使用 |
JP2008061539A (ja) * | 2006-09-05 | 2008-03-21 | Osaka Univ | ヒト補体制御因子発現遺伝子およびその利用 |
WO2016174407A1 (en) * | 2015-04-27 | 2016-11-03 | Ucl Business Plc | Nucleic acid construct for expressing more than one chimeric antigen receptor |
Non-Patent Citations (1)
Title |
---|
MOLECULAR THERAPY : THE JOURNAL OF THE AMERICAN SOCIETY OF GENE THERAPY, vol. 11(4), JPN6022006448, 2005, pages 645 - 651, ISSN: 0004709205 * |
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