JP2020512845A5 - - Google Patents
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- JP2020512845A5 JP2020512845A5 JP2020505541A JP2020505541A JP2020512845A5 JP 2020512845 A5 JP2020512845 A5 JP 2020512845A5 JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020512845 A5 JP2020512845 A5 JP 2020512845A5
- Authority
- JP
- Japan
- Prior art keywords
- sequence
- ato
- universal sequence
- target polynucleotide
- modified
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000000034 method Methods 0.000 claims description 31
- 108091033319 polynucleotide Proteins 0.000 claims description 24
- 102000040430 polynucleotide Human genes 0.000 claims description 24
- 239000002157 polynucleotide Substances 0.000 claims description 24
- 108091034117 Oligonucleotide Proteins 0.000 claims description 17
- 230000000295 complement effect Effects 0.000 claims description 15
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 claims description 10
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 claims description 10
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 230000037452 priming Effects 0.000 claims description 4
- 125000006850 spacer group Chemical group 0.000 claims description 4
- 108091028664 Ribonucleotide Proteins 0.000 claims description 3
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical class O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims description 3
- 239000002336 ribonucleotide Substances 0.000 claims description 3
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 3
- 102000012410 DNA Ligases Human genes 0.000 claims description 2
- 108010061982 DNA Ligases Proteins 0.000 claims description 2
- 125000003277 amino group Chemical group 0.000 claims description 2
- 239000005546 dideoxynucleotide Substances 0.000 claims description 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims 2
- ASJSAQIRZKANQN-CRCLSJGQSA-N 2-deoxy-D-ribose Chemical compound OC[C@@H](O)[C@@H](O)CC=O ASJSAQIRZKANQN-CRCLSJGQSA-N 0.000 claims 1
- 108060002716 Exonuclease Proteins 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims 1
- 230000003321 amplification Effects 0.000 claims 1
- 102000013165 exonuclease Human genes 0.000 claims 1
- 238000003199 nucleic acid amplification method Methods 0.000 claims 1
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 125000003729 nucleotide group Chemical group 0.000 description 3
- 108010068698 spleen exonuclease Proteins 0.000 description 3
- 108020004414 DNA Proteins 0.000 description 2
- 108010010677 Phosphodiesterase I Proteins 0.000 description 2
- 238000003776 cleavage reaction Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 230000007017 scission Effects 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- -1 spacers phosphoramidite Chemical class 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- RYYWUUFWQRZTIU-UHFFFAOYSA-K thiophosphate Chemical compound [O-]P([O-])([O-])=S RYYWUUFWQRZTIU-UHFFFAOYSA-K 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB1706059.1A GB201706059D0 (en) | 2017-04-17 | 2017-04-17 | Methods, compositions, and kits for preparing library and targeted sequencing |
| GB1706059.1 | 2017-04-17 | ||
| GB1708384.1 | 2017-05-25 | ||
| GBGB1708384.1A GB201708384D0 (en) | 2017-05-25 | 2017-05-25 | Methods, compositions, and kits for preparing library and targeted sequencing |
| GB1719114.9 | 2017-11-17 | ||
| GBGB1719114.9A GB201719114D0 (en) | 2017-11-17 | 2017-11-17 | Methods, compositions, and kits for preparing library and targeted sequencing |
| PCT/GB2018/051000 WO2018193233A1 (en) | 2017-04-17 | 2018-04-17 | Methods, compositions, and kits for preparing nucleic acid libraries |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020512845A JP2020512845A (ja) | 2020-04-30 |
| JP2020512845A5 true JP2020512845A5 (enExample) | 2021-04-22 |
| JP7079029B2 JP7079029B2 (ja) | 2022-06-01 |
Family
ID=62089770
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020505541A Active JP7079029B2 (ja) | 2017-04-17 | 2018-04-17 | 核酸ライブラリーを調製するための方法、組成物、およびキット |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US11408025B2 (enExample) |
| EP (1) | EP3612642B1 (enExample) |
| JP (1) | JP7079029B2 (enExample) |
| CN (2) | CN110832084B (enExample) |
| AU (1) | AU2018254190B2 (enExample) |
| CA (1) | CA3060018A1 (enExample) |
| ES (1) | ES3035812T3 (enExample) |
| WO (1) | WO2018193233A1 (enExample) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018193233A1 (en) | 2017-04-17 | 2018-10-25 | Genefirst Ltd | Methods, compositions, and kits for preparing nucleic acid libraries |
| WO2020106906A1 (en) * | 2018-11-21 | 2020-05-28 | Avida Biomed, Inc. | Methods for targeted nucleic acid library formation |
| US11390915B2 (en) * | 2019-04-26 | 2022-07-19 | New England Biolabs, Inc. | Polynucleotide adapter design for reduced bias |
| WO2020252720A1 (zh) * | 2019-06-20 | 2020-12-24 | 深圳华大生命科学研究院 | 基于rna样本构建文库的方法及应用 |
| WO2020264565A1 (en) * | 2019-06-25 | 2020-12-30 | Board Of Regents, The University Of Texas System | Methods for duplex sequencing of cell-free dna and applications thereof |
| US20230227896A1 (en) * | 2020-01-16 | 2023-07-20 | Dnae Diagnostics Limited | Compositions, Kits and Methods for Isolating Target Polynucleotides |
| AU2021270596A1 (en) * | 2020-05-15 | 2022-12-15 | Integrated Dna Technologies, Inc. | Methods for ligation-coupled-PCR |
| MX2023001400A (es) * | 2020-09-11 | 2023-04-25 | Illumina Cambridge Ltd | Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla. |
| GB202108427D0 (en) | 2021-06-14 | 2021-07-28 | Fu Guoliang | Methods, compositions, and kits for preparing sequencing library |
| CN113684249B (zh) * | 2021-08-25 | 2024-10-22 | 广州达安基因股份有限公司 | 一种rna和dna二代测序文库的构建方法及二代测序盒 |
| WO2023077128A1 (en) * | 2021-10-29 | 2023-05-04 | Board Of Regents, The University Of Texas System | Shielded small nucleotides for intracellular barcoding |
| US20250179567A1 (en) * | 2022-02-18 | 2025-06-05 | Agilent Technologies, Inc. | Systems and methods for targeted nucleic acid capture and barcoding |
| CN119095979A (zh) * | 2022-03-09 | 2024-12-06 | 新英格兰生物实验室公司 | 目标富集 |
| CN117487884B (zh) * | 2022-08-01 | 2025-04-15 | 南京诺唯赞生物科技股份有限公司 | 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸 |
| WO2024102958A1 (en) * | 2022-11-11 | 2024-05-16 | Biomeme, Inc. | Methods and compositions for processing and amplification of nucleic acids |
| WO2024137316A1 (en) * | 2022-12-23 | 2024-06-27 | Foundation Medicine, Inc. | Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs |
| GB202308392D0 (en) * | 2023-06-06 | 2023-07-19 | Fu Guoliang | Methods, compositions, and kits for preparing library and targeted sequencing |
| GB202319353D0 (en) | 2023-12-17 | 2024-01-31 | Fu Guoliang | Tail sequence guided addition of adaptor |
| CN117701679B (zh) * | 2024-02-06 | 2024-06-14 | 中国医学科学院基础医学研究所 | 一种基于5’连接的单链dna特异的高通量测序方法 |
| WO2025184067A1 (en) * | 2024-02-26 | 2025-09-04 | Biomeme, Inc. | Methods and compositions for processing and amplification of nucleic acids |
| WO2026050948A1 (zh) * | 2024-09-04 | 2026-03-12 | 深圳华大生命科学研究院 | 核酸文库的构建方法及应用 |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070128598A1 (en) * | 2002-10-30 | 2007-06-07 | Piet Boender | Methods for generating rna copies |
| US20050153333A1 (en) * | 2003-12-02 | 2005-07-14 | Sooknanan Roy R. | Selective terminal tagging of nucleic acids |
| US20120156729A1 (en) * | 2003-12-02 | 2012-06-21 | Sooknanan Roy R | Selective terminal tagging of nucleic acids |
| US8304183B2 (en) * | 2005-11-30 | 2012-11-06 | Cellscript, Inc. | Selective terminal tagging of nucleic acids |
| US7754429B2 (en) * | 2006-10-06 | 2010-07-13 | Illumina Cambridge Limited | Method for pair-wise sequencing a plurity of target polynucleotides |
| EP2280081A1 (en) * | 2009-07-31 | 2011-02-02 | Qiagen GmbH | Method of normalized quantification of RNA |
| KR101599555B1 (ko) | 2009-12-24 | 2016-03-03 | 두산인프라코어 주식회사 | 하이브리드용 전력 변환 장치 |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| EP2971097B1 (en) * | 2013-03-15 | 2018-08-01 | Verinata Health, Inc | Generating cell-free dna libraries directly from blood |
| WO2014176355A1 (en) * | 2013-04-23 | 2014-10-30 | President And Fellows Of Harvard College | In situ interaction determination |
| US20160257985A1 (en) * | 2013-11-18 | 2016-09-08 | Rubicon Genomics, Inc. | Degradable adaptors for background reduction |
| EP3080298B1 (en) * | 2013-12-11 | 2018-10-31 | AccuraGen Holdings Limited | Methods for detecting rare sequence variants |
| US10407720B2 (en) * | 2013-12-15 | 2019-09-10 | Academia Sinica | Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences |
| CN106029962A (zh) * | 2014-02-11 | 2016-10-12 | 豪夫迈·罗氏有限公司 | 靶向测序和uid过滤 |
| EP3626834B1 (en) * | 2014-07-15 | 2022-09-21 | Qiagen Sciences, LLC | Semi-random barcodes for nucleic acid analysis |
| WO2018193233A1 (en) | 2017-04-17 | 2018-10-25 | Genefirst Ltd | Methods, compositions, and kits for preparing nucleic acid libraries |
-
2018
- 2018-04-17 WO PCT/GB2018/051000 patent/WO2018193233A1/en not_active Ceased
- 2018-04-17 EP EP18721095.0A patent/EP3612642B1/en active Active
- 2018-04-17 CN CN201880040163.9A patent/CN110832084B/zh active Active
- 2018-04-17 AU AU2018254190A patent/AU2018254190B2/en active Active
- 2018-04-17 CA CA3060018A patent/CA3060018A1/en active Pending
- 2018-04-17 CN CN202510735693.7A patent/CN120796437A/zh active Pending
- 2018-04-17 ES ES18721095T patent/ES3035812T3/es active Active
- 2018-04-17 US US16/605,520 patent/US11408025B2/en active Active
- 2018-04-17 JP JP2020505541A patent/JP7079029B2/ja active Active
-
2022
- 2022-07-20 US US17/869,595 patent/US11993805B2/en active Active
-
2023
- 2023-06-26 US US18/341,446 patent/US12297484B2/en active Active
-
2025
- 2025-05-12 US US19/204,940 patent/US20250270621A1/en active Pending
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