JP7079029B2 - 核酸ライブラリーを調製するための方法、組成物、およびキット - Google Patents

核酸ライブラリーを調製するための方法、組成物、およびキット Download PDF

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JP7079029B2
JP7079029B2 JP2020505541A JP2020505541A JP7079029B2 JP 7079029 B2 JP7079029 B2 JP 7079029B2 JP 2020505541 A JP2020505541 A JP 2020505541A JP 2020505541 A JP2020505541 A JP 2020505541A JP 7079029 B2 JP7079029 B2 JP 7079029B2
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sequence
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target polynucleotide
primer
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フゥ グオリアン
ダンウェル トーマス
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
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  • Wood Science & Technology (AREA)
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  • Biomedical Technology (AREA)
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  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2020505541A 2017-04-17 2018-04-17 核酸ライブラリーを調製するための方法、組成物、およびキット Active JP7079029B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB1706059.1A GB201706059D0 (en) 2017-04-17 2017-04-17 Methods, compositions, and kits for preparing library and targeted sequencing
GB1706059.1 2017-04-17
GBGB1708384.1A GB201708384D0 (en) 2017-05-25 2017-05-25 Methods, compositions, and kits for preparing library and targeted sequencing
GB1708384.1 2017-05-25
GBGB1719114.9A GB201719114D0 (en) 2017-11-17 2017-11-17 Methods, compositions, and kits for preparing library and targeted sequencing
GB1719114.9 2017-11-17
PCT/GB2018/051000 WO2018193233A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries

Publications (3)

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JP2020512845A JP2020512845A (ja) 2020-04-30
JP2020512845A5 JP2020512845A5 (enExample) 2021-04-22
JP7079029B2 true JP7079029B2 (ja) 2022-06-01

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US (4) US11408025B2 (enExample)
EP (1) EP3612642B1 (enExample)
JP (1) JP7079029B2 (enExample)
CN (2) CN120796437A (enExample)
AU (1) AU2018254190B2 (enExample)
CA (1) CA3060018A1 (enExample)
ES (1) ES3035812T3 (enExample)
WO (1) WO2018193233A1 (enExample)

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EP3612642B1 (en) 2017-04-17 2025-07-09 GeneFirst Limited Methods and kits for preparing nucleic acid libraries
GB2595076B8 (en) * 2018-11-21 2023-08-23 Agilent Technologies Inc Methods for targeted nucleic acid library formation
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
EP3988666B1 (en) * 2019-06-20 2023-10-25 BGI Shenzhen Method for constructing library on basis of rna samples, and use thereof
US20220356467A1 (en) * 2019-06-25 2022-11-10 Board Of Regents, The University Of Texas System Methods for duplex sequencing of cell-free dna and applications thereof
IL300983A (en) * 2020-09-11 2023-04-01 Illumina Cambridge Ltd Methods for enriching a target sequence from a sequence library using hairpin adapters
GB202108427D0 (en) 2021-06-14 2021-07-28 Fu Guoliang Methods, compositions, and kits for preparing sequencing library
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
WO2023077128A1 (en) * 2021-10-29 2023-05-04 Board Of Regents, The University Of Texas System Shielded small nucleotides for intracellular barcoding
CN117487884B (zh) * 2022-08-01 2025-04-15 南京诺唯赞生物科技股份有限公司 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸
WO2024102958A1 (en) * 2022-11-11 2024-05-16 Biomeme, Inc. Methods and compositions for processing and amplification of nucleic acids
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
GB202308392D0 (en) 2023-06-06 2023-07-19 Fu Guoliang Methods, compositions, and kits for preparing library and targeted sequencing
GB202319353D0 (en) 2023-12-17 2024-01-31 Fu Guoliang Tail sequence guided addition of adaptor
CN117701679B (zh) * 2024-02-06 2024-06-14 中国医学科学院基础医学研究所 一种基于5’连接的单链dna特异的高通量测序方法

Citations (4)

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US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
JP2006504440A (ja) 2002-10-30 2006-02-09 パムジーン ベー．ベー． 複数のｒｎａコピーを作成するための改良方法
WO2007062495A1 (en) 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
JP2013500706A (ja) 2009-07-31 2013-01-10 キアゲン ゲーエムベーハー Ｒｎａの正規化した定量化方法

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US20120156729A1 (en) * 2003-12-02 2012-06-21 Sooknanan Roy R Selective terminal tagging of nucleic acids
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
KR101599555B1 (ko) 2009-12-24 2016-03-03 두산인프라코어 주식회사 하이브리드용 전력 변환 장치
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
EP3409791B1 (en) * 2013-03-15 2021-06-30 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014176355A1 (en) * 2013-04-23 2014-10-30 President And Fellows Of Harvard College In situ interaction determination
JP2016537003A (ja) * 2013-11-18 2016-12-01 ルビコン ゲノミクス インコーポレイテッド バックグラウンド減少のための分解可能なアダプター
EP3495506B1 (en) * 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
CN105793438B (zh) * 2013-12-15 2020-02-11 中央研究院 未知序列的双股线性核酸的全长扩增方法
JP6494045B2 (ja) * 2014-02-11 2019-04-03 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 標的シーケンシングおよびｕｉｄフィルタリング
EP3169799B1 (en) * 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
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JP2006504440A (ja) 2002-10-30 2006-02-09 パムジーン ベー．ベー． 複数のｒｎａコピーを作成するための改良方法
US20050153333A1 (en) 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
WO2007062495A1 (en) 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
JP2013500706A (ja) 2009-07-31 2013-01-10 キアゲン ゲーエムベーハー Ｒｎａの正規化した定量化方法

Non-Patent Citations (1)

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Nucleic Acids Research，2016年，Vol. 44, No. 11, e105

Also Published As

Publication number Publication date
WO2018193233A1 (en) 2018-10-25
US20250270621A1 (en) 2025-08-28
CN120796437A (zh) 2025-10-17
US11993805B2 (en) 2024-05-28
CN110832084B (zh) 2025-06-20
ES3035812T3 (en) 2025-09-09
AU2018254190B2 (en) 2024-05-30
US20240002906A1 (en) 2024-01-04
US12297484B2 (en) 2025-05-13
CA3060018A1 (en) 2018-10-25
EP3612642B1 (en) 2025-07-09
AU2018254190A1 (en) 2019-10-31
CN110832084A (zh) 2020-02-21
JP2020512845A (ja) 2020-04-30
EP3612642C0 (en) 2025-07-09
EP3612642A1 (en) 2020-02-26
US20200115736A1 (en) 2020-04-16
US11408025B2 (en) 2022-08-09
US20230121409A1 (en) 2023-04-20

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