CN120796437A - 用于制备核酸文库的方法、组合物和试剂盒 - Google Patents

用于制备核酸文库的方法、组合物和试剂盒

Info

Publication number
CN120796437A
CN120796437A CN202510735693.7A CN202510735693A CN120796437A CN 120796437 A CN120796437 A CN 120796437A CN 202510735693 A CN202510735693 A CN 202510735693A CN 120796437 A CN120796437 A CN 120796437A
Authority
CN
China
Prior art keywords
sequence
ato
target polynucleotide
target
universal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202510735693.7A
Other languages
English (en)
Chinese (zh)
Inventor
富国良
T·邓威尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genefirst Ltd
Original Assignee
Genefirst Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1706059.1A external-priority patent/GB201706059D0/en
Priority claimed from GBGB1708384.1A external-priority patent/GB201708384D0/en
Priority claimed from GBGB1719114.9A external-priority patent/GB201719114D0/en
Application filed by Genefirst Ltd filed Critical Genefirst Ltd
Publication of CN120796437A publication Critical patent/CN120796437A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202510735693.7A 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒 Pending CN120796437A (zh)

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
GBGB1706059.1A GB201706059D0 (en) 2017-04-17 2017-04-17 Methods, compositions, and kits for preparing library and targeted sequencing
GB1706059.1 2017-04-17
GBGB1708384.1A GB201708384D0 (en) 2017-05-25 2017-05-25 Methods, compositions, and kits for preparing library and targeted sequencing
GB1708384.1 2017-05-25
GB1719114.9 2017-11-17
GBGB1719114.9A GB201719114D0 (en) 2017-11-17 2017-11-17 Methods, compositions, and kits for preparing library and targeted sequencing
PCT/GB2018/051000 WO2018193233A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries
CN201880040163.9A CN110832084B (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201880040163.9A Division CN110832084B (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

Publications (1)

Publication Number Publication Date
CN120796437A true CN120796437A (zh) 2025-10-17

Family

ID=62089770

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202510735693.7A Pending CN120796437A (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒
CN201880040163.9A Active CN110832084B (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

Family Applications After (1)

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CN201880040163.9A Active CN110832084B (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

Country Status (8)

Country Link
US (4) US11408025B2 (enExample)
EP (1) EP3612642B1 (enExample)
JP (1) JP7079029B2 (enExample)
CN (2) CN120796437A (enExample)
AU (1) AU2018254190B2 (enExample)
CA (1) CA3060018A1 (enExample)
ES (1) ES3035812T3 (enExample)
WO (1) WO2018193233A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN120796437A (zh) 2017-04-17 2025-10-17 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒
KR20210141915A (ko) * 2018-11-21 2021-11-23 아비다 바이오메드 인코포레이티드 표적화된 핵산 라이브러리 형성을 위한 방법
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
WO2020252720A1 (zh) * 2019-06-20 2020-12-24 深圳华大生命科学研究院 基于rna样本构建文库的方法及应用
WO2020264565A1 (en) * 2019-06-25 2020-12-30 Board Of Regents, The University Of Texas System Methods for duplex sequencing of cell-free dna and applications thereof
ES3004943T3 (en) * 2020-09-11 2025-03-13 Illumina Cambridge Ltd Methods of enriching a target sequence from a sequencing library using hairpin adaptors
GB202108427D0 (en) 2021-06-14 2021-07-28 Fu Guoliang Methods, compositions, and kits for preparing sequencing library
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
WO2023077128A1 (en) * 2021-10-29 2023-05-04 Board Of Regents, The University Of Texas System Shielded small nucleotides for intracellular barcoding
CN117487884B (zh) * 2022-08-01 2025-04-15 南京诺唯赞生物科技股份有限公司 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸
CN120603940A (zh) * 2022-11-11 2025-09-05 生米公司 用于处理和扩增核酸的方法和组合物
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
GB202308392D0 (en) 2023-06-06 2023-07-19 Fu Guoliang Methods, compositions, and kits for preparing library and targeted sequencing
GB202319353D0 (en) 2023-12-17 2024-01-31 Fu Guoliang Tail sequence guided addition of adaptor
CN117701679B (zh) * 2024-02-06 2024-06-14 中国医学科学院基础医学研究所 一种基于5’连接的单链dna特异的高通量测序方法

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AU2003293634A1 (en) * 2002-10-30 2004-06-03 Pamgene B.V. Improved methods for generating multiple rna copies
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US8304183B2 (en) * 2005-11-30 2012-11-06 Cellscript, Inc. Selective terminal tagging of nucleic acids
US20120156729A1 (en) * 2003-12-02 2012-06-21 Sooknanan Roy R Selective terminal tagging of nucleic acids
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
KR101599555B1 (ko) 2009-12-24 2016-03-03 두산인프라코어 주식회사 하이브리드용 전력 변환 장치
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
CA2906818C (en) * 2013-03-15 2022-08-16 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
US10053725B2 (en) * 2013-04-23 2018-08-21 President And Fellows Of Harvard College In situ interaction determination
CA2930942A1 (en) * 2013-11-18 2015-05-21 Rubicon Genomics, Inc. Degradable adaptors for background reduction
KR102379877B1 (ko) * 2013-12-11 2022-03-30 아큐라젠 홀딩스 리미티드 희귀 서열 변이를 검출하기 위한 조성물 및 방법
WO2015089496A1 (en) * 2013-12-15 2015-06-18 Academia Sinica Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
CN106029962A (zh) * 2014-02-11 2016-10-12 豪夫迈·罗氏有限公司 靶向测序和uid过滤
WO2016010856A1 (en) * 2014-07-15 2016-01-21 Qiagen Sciences, Llc Semi-random barcodes for nucleic acid analysis
CN120796437A (zh) 2017-04-17 2025-10-17 吉复生物科技有限公司 用于制备核酸文库的方法、组合物和试剂盒

Also Published As

Publication number Publication date
CN110832084B (zh) 2025-06-20
US20200115736A1 (en) 2020-04-16
ES3035812T3 (en) 2025-09-09
US11408025B2 (en) 2022-08-09
US20240002906A1 (en) 2024-01-04
WO2018193233A1 (en) 2018-10-25
US20250270621A1 (en) 2025-08-28
JP2020512845A (ja) 2020-04-30
EP3612642A1 (en) 2020-02-26
EP3612642B1 (en) 2025-07-09
JP7079029B2 (ja) 2022-06-01
CA3060018A1 (en) 2018-10-25
AU2018254190B2 (en) 2024-05-30
US12297484B2 (en) 2025-05-13
US20230121409A1 (en) 2023-04-20
EP3612642C0 (en) 2025-07-09
CN110832084A (zh) 2020-02-21
US11993805B2 (en) 2024-05-28
AU2018254190A1 (en) 2019-10-31

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