CA3060018A1 - Methods, compositions, and kits for preparing nucleic acid libraries - Google Patents

Methods, compositions, and kits for preparing nucleic acid libraries Download PDF

Info

Publication number
CA3060018A1
CA3060018A1 CA3060018A CA3060018A CA3060018A1 CA 3060018 A1 CA3060018 A1 CA 3060018A1 CA 3060018 A CA3060018 A CA 3060018A CA 3060018 A CA3060018 A CA 3060018A CA 3060018 A1 CA3060018 A1 CA 3060018A1
Authority
CA
Canada
Prior art keywords
ato
sequence
target
primer
universal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3060018A
Other languages
English (en)
French (fr)
Inventor
Thomas DUNWELL
Guoliang Fu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GENEFIRST Ltd
Original Assignee
GENEFIRST Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB1706059.1A external-priority patent/GB201706059D0/en
Priority claimed from GBGB1708384.1A external-priority patent/GB201708384D0/en
Priority claimed from GBGB1719114.9A external-priority patent/GB201719114D0/en
Application filed by GENEFIRST Ltd filed Critical GENEFIRST Ltd
Publication of CA3060018A1 publication Critical patent/CA3060018A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3060018A 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries Pending CA3060018A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GBGB1706059.1A GB201706059D0 (en) 2017-04-17 2017-04-17 Methods, compositions, and kits for preparing library and targeted sequencing
GB1706059.1 2017-04-17
GBGB1708384.1A GB201708384D0 (en) 2017-05-25 2017-05-25 Methods, compositions, and kits for preparing library and targeted sequencing
GB1708384.1 2017-05-25
GBGB1719114.9A GB201719114D0 (en) 2017-11-17 2017-11-17 Methods, compositions, and kits for preparing library and targeted sequencing
GB1719114.9 2017-11-17
PCT/GB2018/051000 WO2018193233A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries

Publications (1)

Publication Number Publication Date
CA3060018A1 true CA3060018A1 (en) 2018-10-25

Family

ID=62089770

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3060018A Pending CA3060018A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries

Country Status (8)

Country Link
US (4) US11408025B2 (enExample)
EP (1) EP3612642B1 (enExample)
JP (1) JP7079029B2 (enExample)
CN (2) CN120796437A (enExample)
AU (1) AU2018254190B2 (enExample)
CA (1) CA3060018A1 (enExample)
ES (1) ES3035812T3 (enExample)
WO (1) WO2018193233A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3612642B1 (en) 2017-04-17 2025-07-09 GeneFirst Limited Methods and kits for preparing nucleic acid libraries
GB2595076B8 (en) * 2018-11-21 2023-08-23 Agilent Technologies Inc Methods for targeted nucleic acid library formation
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
EP3988666B1 (en) * 2019-06-20 2023-10-25 BGI Shenzhen Method for constructing library on basis of rna samples, and use thereof
US20220356467A1 (en) * 2019-06-25 2022-11-10 Board Of Regents, The University Of Texas System Methods for duplex sequencing of cell-free dna and applications thereof
IL300983A (en) * 2020-09-11 2023-04-01 Illumina Cambridge Ltd Methods for enriching a target sequence from a sequence library using hairpin adapters
GB202108427D0 (en) 2021-06-14 2021-07-28 Fu Guoliang Methods, compositions, and kits for preparing sequencing library
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
WO2023077128A1 (en) * 2021-10-29 2023-05-04 Board Of Regents, The University Of Texas System Shielded small nucleotides for intracellular barcoding
CN117487884B (zh) * 2022-08-01 2025-04-15 南京诺唯赞生物科技股份有限公司 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸
WO2024102958A1 (en) * 2022-11-11 2024-05-16 Biomeme, Inc. Methods and compositions for processing and amplification of nucleic acids
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
GB202308392D0 (en) 2023-06-06 2023-07-19 Fu Guoliang Methods, compositions, and kits for preparing library and targeted sequencing
GB202319353D0 (en) 2023-12-17 2024-01-31 Fu Guoliang Tail sequence guided addition of adaptor
CN117701679B (zh) * 2024-02-06 2024-06-14 中国医学科学院基础医学研究所 一种基于5’连接的单链dna特异的高通量测序方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070128598A1 (en) * 2002-10-30 2007-06-07 Piet Boender Methods for generating rna copies
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids
US20120156729A1 (en) * 2003-12-02 2012-06-21 Sooknanan Roy R Selective terminal tagging of nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2280081A1 (en) * 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
KR101599555B1 (ko) 2009-12-24 2016-03-03 두산인프라코어 주식회사 하이브리드용 전력 변환 장치
ES2690753T3 (es) 2010-09-21 2018-11-22 Agilent Technologies, Inc. Aumento de la confianza en las identificaciones de alelos con el recuento molecular
EP3409791B1 (en) * 2013-03-15 2021-06-30 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014176355A1 (en) * 2013-04-23 2014-10-30 President And Fellows Of Harvard College In situ interaction determination
JP2016537003A (ja) * 2013-11-18 2016-12-01 ルビコン ゲノミクス インコーポレイテッド バックグラウンド減少のための分解可能なアダプター
EP3495506B1 (en) * 2013-12-11 2023-07-12 AccuraGen Holdings Limited Methods for detecting rare sequence variants
CN105793438B (zh) * 2013-12-15 2020-02-11 中央研究院 未知序列的双股线性核酸的全长扩增方法
JP6494045B2 (ja) * 2014-02-11 2019-04-03 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 標的シーケンシングおよびｕｉｄフィルタリング
EP3169799B1 (en) * 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
EP3612642B1 (en) 2017-04-17 2025-07-09 GeneFirst Limited Methods and kits for preparing nucleic acid libraries

Also Published As

Publication number Publication date
WO2018193233A1 (en) 2018-10-25
US20250270621A1 (en) 2025-08-28
CN120796437A (zh) 2025-10-17
US11993805B2 (en) 2024-05-28
CN110832084B (zh) 2025-06-20
ES3035812T3 (en) 2025-09-09
AU2018254190B2 (en) 2024-05-30
US20240002906A1 (en) 2024-01-04
US12297484B2 (en) 2025-05-13
EP3612642B1 (en) 2025-07-09
AU2018254190A1 (en) 2019-10-31
JP7079029B2 (ja) 2022-06-01
CN110832084A (zh) 2020-02-21
JP2020512845A (ja) 2020-04-30
EP3612642C0 (en) 2025-07-09
EP3612642A1 (en) 2020-02-26
US20200115736A1 (en) 2020-04-16
US11408025B2 (en) 2022-08-09
US20230121409A1 (en) 2023-04-20

Similar Documents

Publication Publication Date Title
US12297484B2 (en) Methods, compositions, and kits for preparing nucleic acid libraries
AU2022201907B2 (en) Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions
US11014957B2 (en) Methods of library construction for polynucleotide sequencing
CA2948951C (en) Synthesis of double-stranded nucleic acids
JP2016511007A (ja) 鎖になったｒｎａまたはｄｎａのライブラリを生成するための方法、組成物およびキット
US20240384261A1 (en) Methods, compositions, and kits for preparing sequencing library
WO2024252146A1 (en) Methods, compositions, and kits for preparing library and targeted sequencing
WO2025133584A1 (en) Tail sequence guided addition of adaptor

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20230417

EEER Examination request

Effective date: 20230417

EEER Examination request

Effective date: 20230417