CN110832084B - 用于制备核酸文库的方法、组合物和试剂盒 - Google Patents

用于制备核酸文库的方法、组合物和试剂盒 Download PDF

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Publication number
CN110832084B
CN110832084B CN201880040163.9A CN201880040163A CN110832084B CN 110832084 B CN110832084 B CN 110832084B CN 201880040163 A CN201880040163 A CN 201880040163A CN 110832084 B CN110832084 B CN 110832084B
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sequence
ato
target
target polynucleotide
universal
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CN110832084A (zh
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富国良
T·邓威尔
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Genefirst Ltd
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Genefirst Ltd
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Priority claimed from GBGB1706059.1A external-priority patent/GB201706059D0/en
Priority claimed from GBGB1708384.1A external-priority patent/GB201708384D0/en
Priority claimed from GBGB1719114.9A external-priority patent/GB201719114D0/en
Application filed by Genefirst Ltd filed Critical Genefirst Ltd
Priority to CN202510735693.7A priority Critical patent/CN120796437A/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN201880040163.9A 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒 Active CN110832084B (zh)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202510735693.7A CN120796437A (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
GB1706059.1 2017-04-17
GBGB1706059.1A GB201706059D0 (en) 2017-04-17 2017-04-17 Methods, compositions, and kits for preparing library and targeted sequencing
GBGB1708384.1A GB201708384D0 (en) 2017-05-25 2017-05-25 Methods, compositions, and kits for preparing library and targeted sequencing
GB1708384.1 2017-05-25
GB1719114.9 2017-11-17
GBGB1719114.9A GB201719114D0 (en) 2017-11-17 2017-11-17 Methods, compositions, and kits for preparing library and targeted sequencing
PCT/GB2018/051000 WO2018193233A1 (en) 2017-04-17 2018-04-17 Methods, compositions, and kits for preparing nucleic acid libraries

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CN202510735693.7A Division CN120796437A (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

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CN110832084A CN110832084A (zh) 2020-02-21
CN110832084B true CN110832084B (zh) 2025-06-20

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CN202510735693.7A Pending CN120796437A (zh) 2017-04-17 2018-04-17 用于制备核酸文库的方法、组合物和试剂盒

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US (4) US11408025B2 (enExample)
EP (1) EP3612642B1 (enExample)
JP (1) JP7079029B2 (enExample)
CN (2) CN110832084B (enExample)
AU (1) AU2018254190B2 (enExample)
CA (1) CA3060018A1 (enExample)
ES (1) ES3035812T3 (enExample)
WO (1) WO2018193233A1 (enExample)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2018254190B2 (en) 2017-04-17 2024-05-30 Guoliang Fu Methods, compositions, and kits for preparing nucleic acid libraries
AU2019384154B2 (en) * 2018-11-21 2025-11-20 Agilent Technologies, Inc. Methods for targeted nucleic acid library formation
US11390915B2 (en) * 2019-04-26 2022-07-19 New England Biolabs, Inc. Polynucleotide adapter design for reduced bias
CN114026249B (zh) 2019-06-20 2024-01-23 深圳华大生命科学研究院 基于rna样本构建文库的方法及应用
US20220356467A1 (en) * 2019-06-25 2022-11-10 Board Of Regents, The University Of Texas System Methods for duplex sequencing of cell-free dna and applications thereof
EP4342999B1 (en) * 2020-01-16 2025-06-11 DNAe Diagnostics Limited Compositions, kits and methods for isolating target polynucleotides
WO2021232023A2 (en) * 2020-05-15 2021-11-18 Swift Biosciences, Inc. Methods for ligation-coupled-pcr
CA3188197A1 (en) * 2020-09-11 2022-03-17 Andrew Slatter Methods of enriching a target sequence from a sequencing library using hairpin adaptors
GB202108427D0 (en) 2021-06-14 2021-07-28 Fu Guoliang Methods, compositions, and kits for preparing sequencing library
CN113684249B (zh) * 2021-08-25 2024-10-22 广州达安基因股份有限公司 一种rna和dna二代测序文库的构建方法及二代测序盒
WO2023077128A1 (en) * 2021-10-29 2023-05-04 Board Of Regents, The University Of Texas System Shielded small nucleotides for intracellular barcoding
US20250179567A1 (en) * 2022-02-18 2025-06-05 Agilent Technologies, Inc. Systems and methods for targeted nucleic acid capture and barcoding
US20250179568A1 (en) * 2022-03-09 2025-06-05 New England Biolabs, Inc. Target Enrichment
US20250382607A1 (en) * 2022-05-17 2025-12-18 Nanjing GenScript Biotech Co., Ltd. Sgrna sequencing linker and use thereof
CN117487884B (zh) * 2022-08-01 2025-04-15 南京诺唯赞生物科技股份有限公司 一种高样品利用率的基于转座酶的测序文库构建方法及相关寡核苷酸
CN120603940A (zh) * 2022-11-11 2025-09-05 生米公司 用于处理和扩增核酸的方法和组合物
WO2024137316A1 (en) * 2022-12-23 2024-06-27 Foundation Medicine, Inc. Oligonucleotides and methods for capturing single-stranded templates and/or templates with 3' overhangs
GB202308392D0 (en) 2023-06-06 2023-07-19 Fu Guoliang Methods, compositions, and kits for preparing library and targeted sequencing
GB202319353D0 (en) 2023-12-17 2024-01-31 Fu Guoliang Tail sequence guided addition of adaptor
CN117701679B (zh) 2024-02-06 2024-06-14 中国医学科学院基础医学研究所 一种基于5’连接的单链dna特异的高通量测序方法
WO2025184067A1 (en) * 2024-02-26 2025-09-04 Biomeme, Inc. Methods and compositions for processing and amplification of nucleic acids
WO2026050948A1 (zh) * 2024-09-04 2026-03-12 深圳华大生命科学研究院 核酸文库的构建方法及应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2501983A1 (en) 2002-10-30 2004-05-27 Pamgene B.V. Improved methods for generating multiple rna copies
US20120156729A1 (en) * 2003-12-02 2012-06-21 Sooknanan Roy R Selective terminal tagging of nucleic acids
US20050153333A1 (en) * 2003-12-02 2005-07-14 Sooknanan Roy R. Selective terminal tagging of nucleic acids
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
EP2280081A1 (en) 2009-07-31 2011-02-02 Qiagen GmbH Method of normalized quantification of RNA
KR101599555B1 (ko) 2009-12-24 2016-03-03 두산인프라코어 주식회사 하이브리드용 전력 변환 장치
EP2619327B1 (en) 2010-09-21 2014-10-22 Population Genetics Technologies LTD. Increasing confidence of allele calls with molecular counting
WO2014145078A1 (en) * 2013-03-15 2014-09-18 Verinata Health, Inc. Generating cell-free dna libraries directly from blood
WO2014176355A1 (en) * 2013-04-23 2014-10-30 President And Fellows Of Harvard College In situ interaction determination
CN105917002A (zh) * 2013-11-18 2016-08-31 鲁比康基因组学公司 用于减少背景的可降解适体
IL274464B2 (en) * 2013-12-11 2023-08-01 Accuragen Holdings Ltd Preparations and methods for the detection of rare sequence variants
US10407720B2 (en) * 2013-12-15 2019-09-10 Academia Sinica Methods for full-length amplification of double-stranded linear nucleic acids of unknown sequences
EP3105349B1 (en) * 2014-02-11 2020-07-15 F. Hoffmann-La Roche AG Targeted sequencing and uid filtering
EP3169799B1 (en) * 2014-07-15 2019-09-04 Qiagen Sciences, LLC Semi-random barcodes for nucleic acid analysis
AU2018254190B2 (en) 2017-04-17 2024-05-30 Guoliang Fu Methods, compositions, and kits for preparing nucleic acid libraries

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007062495A1 (en) * 2005-11-30 2007-06-07 Roy Rabindranauth Sooknanan Selective terminal tagging of nucleic acids

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US11993805B2 (en) 2024-05-28
EP3612642B1 (en) 2025-07-09
ES3035812T3 (en) 2025-09-09
US20230121409A1 (en) 2023-04-20
US12297484B2 (en) 2025-05-13
EP3612642C0 (en) 2025-07-09
US11408025B2 (en) 2022-08-09
JP2020512845A (ja) 2020-04-30
US20200115736A1 (en) 2020-04-16
JP7079029B2 (ja) 2022-06-01
CA3060018A1 (en) 2018-10-25
US20240002906A1 (en) 2024-01-04
CN120796437A (zh) 2025-10-17
AU2018254190A1 (en) 2019-10-31
AU2018254190B2 (en) 2024-05-30
WO2018193233A1 (en) 2018-10-25
EP3612642A1 (en) 2020-02-26
CN110832084A (zh) 2020-02-21
US20250270621A1 (en) 2025-08-28

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