JP2020511629A5 - - Google Patents

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JP2020511629A5
JP2020511629A5 JP2019543321A JP2019543321A JP2020511629A5 JP 2020511629 A5 JP2020511629 A5 JP 2020511629A5 JP 2019543321 A JP2019543321 A JP 2019543321A JP 2019543321 A JP2019543321 A JP 2019543321A JP 2020511629 A5 JP2020511629 A5 JP 2020511629A5
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oligonucleotide
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JP2020511629A (ja
JP7248368B2 (ja
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JP2019543321A 2017-03-01 2018-02-20 高度に特異的な環状近接ライゲーションアッセイ Active JP7248368B2 (ja)

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Application Number Priority Date Filing Date Title
US201762465320P 2017-03-01 2017-03-01
US62/465,320 2017-03-01
PCT/US2018/018859 WO2018160397A1 (en) 2017-03-01 2018-02-20 Highly specific circular proximity ligation assay

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JP2020511629A JP2020511629A (ja) 2020-04-16
JP2020511629A5 true JP2020511629A5 (enExample) 2021-04-01
JP7248368B2 JP7248368B2 (ja) 2023-03-29

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US (2) US11530438B2 (enExample)
EP (1) EP3589750B1 (enExample)
JP (1) JP7248368B2 (enExample)
CA (1) CA3053384A1 (enExample)
ES (1) ES2917500T3 (enExample)
WO (1) WO2018160397A1 (enExample)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4282977B1 (en) 2015-04-10 2024-06-05 10x Genomics Sweden AB Spatially distinguished, multiplex nucleic acid analysis of biological specimens
KR102476709B1 (ko) 2016-11-21 2022-12-09 나노스트링 테크놀로지스, 인크. 화학적 조성물 및 이것을 사용하는 방법
EP3794146B1 (en) 2018-05-14 2025-12-10 Bruker Spatial Biology, Inc. Method for identifying a predetermined nucleotide sequence
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
CN112752850A (zh) * 2018-09-26 2021-05-04 蓝普臻公司 用于蛋白质检测的数字化扩增
WO2020106906A1 (en) * 2018-11-21 2020-05-28 Avida Biomed, Inc. Methods for targeted nucleic acid library formation
WO2020123311A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Resolving spatial arrays using deconvolution
US12529094B2 (en) 2018-12-10 2026-01-20 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
EP4055185A1 (en) 2019-11-08 2022-09-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
WO2021113290A1 (en) * 2019-12-03 2021-06-10 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
CN115135984B (zh) 2019-12-23 2025-12-23 10X基因组学有限公司 可逆固定试剂及其使用方法
US12241890B2 (en) 2019-12-23 2025-03-04 10X Genomics, Inc. Methods for generating barcoded nucleic acid molecules using fixed cells
DK3891300T3 (da) 2019-12-23 2023-05-22 10X Genomics Inc Fremgangsmåder til rumlig analyse ved anvendelse af rna-template-ligering
US12365942B2 (en) 2020-01-13 2025-07-22 10X Genomics, Inc. Methods of decreasing background on a spatial array
US12405264B2 (en) 2020-01-17 2025-09-02 10X Genomics, Inc. Electrophoretic system and method for analyte capture
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US20210230681A1 (en) 2020-01-24 2021-07-29 10X Genomics, Inc. Methods for spatial analysis using proximity ligation
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11835462B2 (en) 2020-02-11 2023-12-05 10X Genomics, Inc. Methods and compositions for partitioning a biological sample
US12399123B1 (en) 2020-02-14 2025-08-26 10X Genomics, Inc. Spatial targeting of analytes
US12281357B1 (en) 2020-02-14 2025-04-22 10X Genomics, Inc. In situ spatial barcoding
US12215379B2 (en) 2020-02-17 2025-02-04 10X Genomics, Inc. In situ analysis of chromatin interaction
KR20220142501A (ko) * 2020-02-18 2022-10-21 에이전시 포 사이언스, 테크놀로지 앤드 리서치 핵산 프로브
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
US11926863B1 (en) 2020-02-27 2024-03-12 10X Genomics, Inc. Solid state single cell method for analyzing fixed biological cells
US11768175B1 (en) 2020-03-04 2023-09-26 10X Genomics, Inc. Electrophoretic methods for spatial analysis
ES3014967T3 (en) 2020-04-22 2025-04-28 10X Genomics Inc Methods for spatial analysis using targeted rna depletion
US12416603B2 (en) 2020-05-19 2025-09-16 10X Genomics, Inc. Electrophoresis cassettes and instrumentation
EP4414459B1 (en) 2020-05-22 2025-09-03 10X Genomics, Inc. Simultaneous spatio-temporal measurement of gene expression and cellular activity
EP4153776B1 (en) 2020-05-22 2025-03-05 10X Genomics, Inc. Spatial analysis to detect sequence variants
US12265079B1 (en) 2020-06-02 2025-04-01 10X Genomics, Inc. Systems and methods for detecting analytes from captured single biological particles
EP4158054B1 (en) 2020-06-02 2025-04-16 10X Genomics, Inc. Spatial transcriptomics for antigen-receptors
EP4025692A2 (en) 2020-06-02 2022-07-13 10X Genomics, Inc. Nucleic acid library methods
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252747A1 (en) 2020-06-10 2021-12-16 1Ox Genomics, Inc. Fluid delivery methods
US12435363B1 (en) 2020-06-10 2025-10-07 10X Genomics, Inc. Materials and methods for spatial transcriptomics
EP4450639B1 (en) 2020-06-25 2025-10-15 10X Genomics, Inc. Spatial analysis of dna methylation
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US12209280B1 (en) 2020-07-06 2025-01-28 10X Genomics, Inc. Methods of identifying abundance and location of an analyte in a biological sample using second strand synthesis
US12553898B1 (en) 2020-08-10 2026-02-17 10X Genomics, Inc. Fluorescent hybridization of antibody-oligonucleotide for multiplexing and signal amplification
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
EP4214678B1 (en) 2020-09-18 2024-11-06 10X Genomics, Inc. Sample handling apparatus and image registration methods
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) * 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
WO2022109496A2 (en) 2020-11-23 2022-05-27 Pleno, Inc. Encoded assays
WO2022140028A1 (en) 2020-12-21 2022-06-30 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
WO2022178267A2 (en) 2021-02-19 2022-08-25 10X Genomics, Inc. Modular assay support devices
WO2022198068A1 (en) 2021-03-18 2022-09-22 10X Genomics, Inc. Multiplex capture of gene and protein expression from a biological sample
EP4305196B1 (en) 2021-04-14 2025-04-02 10X Genomics, Inc. Methods of measuring mislocalization of an analyte
WO2022236054A1 (en) 2021-05-06 2022-11-10 10X Genomics, Inc. Methods for increasing resolution of spatial analysis
CN113528611A (zh) * 2021-06-01 2021-10-22 上海交通大学 一种蛋白检测试剂盒及检测方法
EP4582555A3 (en) 2021-06-03 2025-10-22 10X Genomics, Inc. Methods, compositions, kits, and systems for enhancing analyte capture for spatial analysis
JP2024527509A (ja) 2021-06-24 2024-07-25 モールキュレント エービー 平面生体試料の空間解析
US12553805B2 (en) 2021-08-02 2026-02-17 10X Genomics, Inc. Methods of preserving a biological sample
CA3223731A1 (en) * 2021-08-11 2023-02-16 Illumina, Inc. Detection of analytes using targeted epigenetic assays, proximity-induced tagmentation, strand invasion, restriction, or ligation
EP4446426B1 (en) 2021-08-16 2026-03-04 10X Genomics, Inc. Probes comprising a split barcode region and methods of use
EP4196605B1 (en) 2021-09-01 2024-12-04 10X Genomics, Inc. Methods for blocking a capture probe on a spatial array
WO2023086880A1 (en) 2021-11-10 2023-05-19 10X Genomics, Inc. Methods, compositions, and kits for determining the location of an analyte in a biological sample
WO2023102118A2 (en) 2021-12-01 2023-06-08 10X Genomics, Inc. Methods, compositions, and systems for improved in situ detection of analytes and spatial analysis
EP4441711A1 (en) 2021-12-20 2024-10-09 10X Genomics, Inc. Self-test for pathology/histology slide imaging device
US20250101503A1 (en) * 2022-01-26 2025-03-27 Moleculent Ab Detection of proximity assay products in situ
US20250180545A1 (en) * 2022-02-14 2025-06-05 Cz Biohub Sf, Llc A tunable proximity assay that can overcome dilutional non-linearity
EP4482979B1 (en) 2022-11-09 2025-10-01 10X Genomics, Inc. Methods, compositions, and kits for determining the location of multiple analytes in a biological sample
EP4623094A1 (en) * 2022-11-23 2025-10-01 Range Biotechnologies, Inc. Compositions and methods for detection of protein analytes
WO2025111396A1 (en) 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Molecular barcode set and use thereof in multiplex proximity detection assays
WO2025111397A1 (en) * 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Use of autoinhibition standard curves in proximity assays
WO2025111398A1 (en) * 2023-11-20 2025-05-30 Alamar Biosciences, Inc. Reduction of non-cognate signal in multiplex proximity ligation assays
CN119757755B (zh) * 2024-11-01 2025-08-29 上海伯杰医疗科技股份有限公司 一种环状连接超敏免疫检测方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5948902A (en) 1997-11-20 1999-09-07 South Alabama Medical Science Foundation Antisense oligonucleotides to human serine/threonine protein phosphatase genes
CN100436574C (zh) 2001-09-06 2008-11-26 基因描绘系统有限公司 复制细胞的快速检测
US20040248103A1 (en) 2003-06-04 2004-12-09 Feaver William John Proximity-mediated rolling circle amplification
US20050233340A1 (en) 2004-04-20 2005-10-20 Barrett Michael T Methods and compositions for assessing CpG methylation
GB0605584D0 (en) 2006-03-20 2006-04-26 Olink Ab Method for analyte detection using proximity probes
GB201004292D0 (en) 2010-03-15 2010-04-28 Olink Ab Assay for localised detection of analytes
GB201107863D0 (en) * 2011-05-11 2011-06-22 Olink Ab Method and product
GB201108678D0 (en) 2011-05-24 2011-07-06 Olink Ab Multiplexed proximity ligation assay
JP2018512164A (ja) * 2015-04-15 2018-05-17 プロメディオール, インコーポレイテッド 骨髄増殖性障害を処置するための方法
US11168347B2 (en) * 2016-09-23 2021-11-09 California Institute Of Technology Digital quantification of DNA replication and/or chromosome segregation based determination of antimicrobial susceptibility

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