JP2020503059A5 - - Google Patents
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- JP2020503059A5 JP2020503059A5 JP2019537102A JP2019537102A JP2020503059A5 JP 2020503059 A5 JP2020503059 A5 JP 2020503059A5 JP 2019537102 A JP2019537102 A JP 2019537102A JP 2019537102 A JP2019537102 A JP 2019537102A JP 2020503059 A5 JP2020503059 A5 JP 2020503059A5
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- WQZGKKKJIJFFOK-IVMDWMLBSA-N D-allose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@H](O)[C@@H]1O WQZGKKKJIJFFOK-IVMDWMLBSA-N 0.000 claims 34
- 239000010452 phosphate Substances 0.000 claims 34
- GSXOAOHZAIYLCY-HSUXUTPPSA-N keto-D-fructose 6-phosphate Chemical compound OCC(=O)[C@@H](O)[C@H](O)[C@H](O)COP(O)(O)=O GSXOAOHZAIYLCY-HSUXUTPPSA-N 0.000 claims 16
- 229940045189 Glucose-6-Phosphate Drugs 0.000 claims 10
- NBSCHQHZLSJFNQ-GASJEMHNSA-N Glucose-6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 claims 10
- 241000193448 Ruminiclostridium thermocellum Species 0.000 claims 10
- 239000006166 lysate Substances 0.000 claims 10
- -1 allose compound Chemical class 0.000 claims 8
- 230000003197 catalytic Effects 0.000 claims 8
- 241001468177 Brevibacillus thermoruber Species 0.000 claims 7
- 108050004944 Alpha-glucan phosphorylase Proteins 0.000 claims 6
- 235000014633 carbohydrates Nutrition 0.000 claims 6
- 239000008103 glucose Substances 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 6
- 150000007523 nucleic acids Chemical group 0.000 claims 6
- 108010077004 EC 2.4.1.49 Proteins 0.000 claims 5
- 229920001850 Nucleic acid sequence Polymers 0.000 claims 5
- 230000000813 microbial Effects 0.000 claims 5
- 108090000790 Enzymes Proteins 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 4
- BJHIKXHVCXFQLS-UYFOZJQFSA-N Fructose Natural products OC[C@@H](O)[C@@H](O)[C@H](O)C(=O)CO BJHIKXHVCXFQLS-UYFOZJQFSA-N 0.000 claims 4
- 238000006555 catalytic reaction Methods 0.000 claims 4
- 238000006243 chemical reaction Methods 0.000 claims 4
- WQZGKKKJIJFFOK-GASJEMHNSA-N D-Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims 3
- 102000009569 Phosphoglucomutase Human genes 0.000 claims 3
- 108010009450 Phosphoglucomutase Proteins 0.000 claims 3
- 241000193446 Thermoanaerobacterium thermosaccharolyticum Species 0.000 claims 3
- 241000206213 Thermosipho africanus Species 0.000 claims 3
- 241000893512 Aquifex aeolicus Species 0.000 claims 2
- 241001157085 Caldanaerobacter subterraneus Species 0.000 claims 2
- 241001503799 Deferribacter desulfuricans Species 0.000 claims 2
- 241001261380 Defluviitalea phaphyphila Species 0.000 claims 2
- 239000005715 Fructose Substances 0.000 claims 2
- 108010028688 Isoamylase Proteins 0.000 claims 2
- 241000168442 Kosmotoga olearia Species 0.000 claims 2
- 241000252565 Pseudothermotoga thermarum Species 0.000 claims 2
- 241000589887 Spirochaeta thermophila Species 0.000 claims 2
- 241000202694 Thermoanaerobacter wiegelii Species 0.000 claims 2
- 241000689179 Thermosipho atlanticus Species 0.000 claims 2
- 241000531145 Thermosphaera aggregans Species 0.000 claims 2
- 241000204652 Thermotoga Species 0.000 claims 2
- 241000204664 Thermotoga neapolitana Species 0.000 claims 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K [O-]P([O-])([O-])=O Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims 2
- 229950010772 glucose-1-phosphate Drugs 0.000 claims 2
- HXXFSFRBOHSIMQ-VFUOTHLCSA-N α-D-glucose 1-phosphate Chemical compound OC[C@H]1O[C@H](OP(O)(O)=O)[C@H](O)[C@@H](O)[C@@H]1O HXXFSFRBOHSIMQ-VFUOTHLCSA-N 0.000 claims 2
- 241001134630 Acidothermus cellulolyticus Species 0.000 claims 1
- 241000212079 Aciduliprofundum Species 0.000 claims 1
- 241000147155 Ammonifex degensii Species 0.000 claims 1
- 241000205042 Archaeoglobus fulgidus Species 0.000 claims 1
- 241001055473 Archaeoglobus sulfaticallidus Species 0.000 claims 1
- 241000593342 Archaeoglobus veneficus Species 0.000 claims 1
- 241000349352 Ardenticatena maritima Species 0.000 claims 1
- 241000984226 Bacillus flavocaldarius Species 0.000 claims 1
- 241000193422 Bacillus lentus Species 0.000 claims 1
- 241000606125 Bacteroides Species 0.000 claims 1
- 241000606215 Bacteroides vulgatus Species 0.000 claims 1
- 241001429558 Caldicellulosiruptor bescii Species 0.000 claims 1
- 241000987688 Caldicellulosiruptor kronotskyensis Species 0.000 claims 1
- 241000670995 Caldisalinibacter kiritimatiensis Species 0.000 claims 1
- 241001397935 Caloranaerobacter ferrireducens Species 0.000 claims 1
- 241000398179 Caminicella sporogenes Species 0.000 claims 1
- 229920002299 Cellodextrin Polymers 0.000 claims 1
- 241001052484 Crenarchaeota archaeon Species 0.000 claims 1
- 241001332002 Deinococcus geothermalis DSM 11300 Species 0.000 claims 1
- 241000592830 Desulfotomaculum kuznetsovii Species 0.000 claims 1
- 229920001353 Dextrin Polymers 0.000 claims 1
- 239000004375 Dextrin Substances 0.000 claims 1
- 241000863389 Dictyoglomus thermophilum Species 0.000 claims 1
- 241000531185 Ferroglobus placidus Species 0.000 claims 1
- 241000346754 Fervidobacterium pennivorans Species 0.000 claims 1
- 241000182950 Geoglobus ahangari Species 0.000 claims 1
- 229920002527 Glycogen Polymers 0.000 claims 1
- 229940096919 Glycogen Drugs 0.000 claims 1
- BYSGBSNPRWKUQH-UJDJLXLFSA-N Glycogen Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)O)[C@H](O)[C@@H](O)[C@@H](O[C@@H]2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)O1 BYSGBSNPRWKUQH-UJDJLXLFSA-N 0.000 claims 1
- 241001026343 Hydrogenivirga Species 0.000 claims 1
- 241000605325 Hydrogenobacter thermophilus Species 0.000 claims 1
- 241000356800 Ignisphaera aggregans Species 0.000 claims 1
- 229920002774 Maltodextrin Polymers 0.000 claims 1
- 239000005913 Maltodextrin Substances 0.000 claims 1
- 241001357706 Marinitoga piezophila Species 0.000 claims 1
- 241000589496 Meiothermus ruber Species 0.000 claims 1
- 241000954246 Meiothermus timidus Species 0.000 claims 1
- 241001326159 Metallosphaera hakonensis Species 0.000 claims 1
- 241000203407 Methanocaldococcus jannaschii Species 0.000 claims 1
- 241000605702 Methanocaldococcus villosus Species 0.000 claims 1
- 241001302042 Methanothermobacter thermautotrophicus Species 0.000 claims 1
- 241001302037 Methanothermobacter wolfeii Species 0.000 claims 1
- 241000010755 Methanothermococcus okinawensis Species 0.000 claims 1
- 241000203367 Methanothermus fervidus Species 0.000 claims 1
- 241001115911 Oceanithermus profundus Species 0.000 claims 1
- 241000037202 Palaeococcus pacificus Species 0.000 claims 1
- 241000981393 Persephonella marina Species 0.000 claims 1
- 241000983938 Petrotoga mobilis Species 0.000 claims 1
- BJHIKXHVCXFQLS-PUFIMZNGSA-N Psicose Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)C(=O)CO BJHIKXHVCXFQLS-PUFIMZNGSA-N 0.000 claims 1
- 241000205160 Pyrococcus Species 0.000 claims 1
- 241001222730 Pyrococcus horikoshii OT3 Species 0.000 claims 1
- 241001542965 Pyrococcus kukulkanii Species 0.000 claims 1
- 241000203746 Sphaerobacter Species 0.000 claims 1
- 229920002472 Starch Polymers 0.000 claims 1
- 241000205098 Sulfolobus acidocaldarius Species 0.000 claims 1
- 241000160715 Sulfolobus tokodaii Species 0.000 claims 1
- 241000981396 Sulfurihydrogenibium azorense Species 0.000 claims 1
- 241000207197 Symbiobacterium thermophilum Species 0.000 claims 1
- 241001147775 Thermoanaerobacter brockii Species 0.000 claims 1
- 241000186337 Thermoanaerobacter ethanolicus Species 0.000 claims 1
- 241000203780 Thermobifida fusca Species 0.000 claims 1
- 241000545779 Thermococcus barophilus Species 0.000 claims 1
- 241001235254 Thermococcus kodakarensis Species 0.000 claims 1
- 241000205180 Thermococcus litoralis Species 0.000 claims 1
- 241000706981 Thermococcus sibiricus Species 0.000 claims 1
- 241001293534 Thermocrinis ruber Species 0.000 claims 1
- 241000204315 Thermosipho <sea snail> Species 0.000 claims 1
- 241000974459 Thermosulfidibacter takaii Species 0.000 claims 1
- 241000869609 Thermosulfurimonas dismutans Species 0.000 claims 1
- 241000204666 Thermotoga maritima Species 0.000 claims 1
- 241001135650 Thermotoga sp. Species 0.000 claims 1
- 241000088680 Thermus islandicus Species 0.000 claims 1
- 241000557726 Thermus oshimai Species 0.000 claims 1
- 241001351214 [Clostridium] straminisolvens Species 0.000 claims 1
- 239000002253 acid Substances 0.000 claims 1
- 235000019425 dextrin Nutrition 0.000 claims 1
- 229940035034 maltodextrin Drugs 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 235000019698 starch Nutrition 0.000 claims 1
- 239000008107 starch Substances 0.000 claims 1
Claims (21)
- アルロ−ス化合物を生産する方法であって、以下のステップ:
多量体グルコ−ス炭水化物を、α−グルカンまたはセロデキストリンホスホリラ−ゼによる触媒作用によりグルコ−ス1−リン酸(G1P)に変換すること;該グルコ−ス1−リン酸(G1P)を、ホスホグルコムタ−ゼによる触媒作用により変換してグルコ−ス6−リン酸(G6P)を生産すること;該グルコ−ス6−リン酸(G6P)を、ホスホグルコイソメラ−ゼによる触媒作用によりフルクト−ス6−リン酸(F6P)に変換すること;該フルクト−ス6−リン酸(F6P)を、アルロ−ス6−リン酸エピメラ−ゼ(A6PE)による触媒作用により変換してアルロ−ス6−リン酸(A6P)を生産すること;および、該アルロ−ス6−リン酸(A6P)を、アルロ−ス6−リン酸ホスファタ−ゼ(A6PP)による触媒作用によりアルロ−スに変換すること、ここでA6PPはRuminiclostridium thermocellum A6PPである、
を含む、前記方法。 - アルロ−ス化合物を生産する方法であって、以下のステップ:
多量体グルコ−ス炭水化物を、α−グルカンまたはセロデキストリンホスホリラ−ゼによる触媒作用によりグルコ−ス1−リン酸(G1P)に変換すること;該グルコ−ス1−リン酸(G1P)を、ホスホグルコムタ−ゼによる触媒作用により変換してグルコ−ス6−リン酸(G6P)を生産すること;該グルコ−ス6−リン酸(G6P)を、ホスホグルコイソメラ−ゼによる触媒作用によりフルクト−ス6−リン酸(F6P)に変換すること;該フルクト−ス6−リン酸(F6P)を、アルロ−ス6−リン酸エピメラ−ゼ(A6PE)による触媒作用により変換してアルロ−ス6−リン酸(A6P)を生産すること;および、該アルロ−ス6−リン酸(A6P)を、アルロ−ス6−リン酸ホスファタ−ゼ(A6PP)による触媒作用によりアルロ−スに変換すること、ここでA6PEはBrevibacillus thermoruberA6PEである、
を含む、前記方法。 - アルロ−ス化合物を生産する方法であって、以下のステップ:
フルクト−ス6−リン酸(F6P)を、Thermoanaerobacterium thermosaccharolyticumA6PE、Hydrogenivirga sp. 128−5−R1−1 A6PE、およびBrevibacillus thermoruberA6PEからなる群から選択されるアルロ−ス6−リン酸エピメラ−ゼ(A6PE)による触媒作用によりアルロ−ス6−リン酸(A6P)に変換すること;および、A6Pを、Ruminiclostridium thermocellumアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)による触媒作用によりアルロ−スに変換すること、
を含む、前記方法。 - アルロ−ス化合物を生産する方法であって、以下のステップ:
フルクト−ス6−リン酸(F6P)を、アルロ−ス6−リン酸エピメラ−ゼ(A6PE)による触媒作用によりアルロ−ス6−リン酸(A6P)に変換すること、ここでA6PEは微生物細胞において発現される核酸配列によってコードされており;および、A6Pを、Ruminiclostridium thermocellumアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)による触媒作用によりアルロ−スに変換すること、
を含む、前記方法。 - アルロ−ス化合物を生産する方法であって、以下のステップ:
アルロ−ス6−リン酸エピメラ−ゼ(A6PE)およびアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)を発現する微生物細胞からの細胞ライセートを含む細胞ライセート混合物において、フルクト−ス6−リン酸(F6P)を、A6PEによる触媒作用によりアルロ−ス6−リン酸(A6P)に変換すること、およびA6PをA6PPによる触媒作用によりアルロースに変換すること、ここでA6PPはRuminiclostridium thermocellum核酸配列によってコードされる、
を含む、前記方法。 - 細胞ライセート混合物において、多量体グルコ−ス炭水化物をアルロ−スに変換すること、ここで細胞ライセート混合物は、α−グルカンホスホリラ−ゼまたはセロデキストリンホスホリラ−ゼ、ホスホグルコムタ−ゼ、ホスホグルコイソメラ−ゼ、アルロ−ス6−リン酸エピメラ−ゼ(A6PE)、およびアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)、ここでA6PPはRuminiclostridium thermocellum核酸配列によってコードされる、を発現する改変微生物細胞からの細胞ライセートを含む、
を含む方法。 - アルロ−スを生産する方法であって、方法が以下:
フルクト−ス6−リン酸(F6P)を、Brevibacillus thermoruberアルロ−ス6−リン酸エピメラ−ゼ(A6PE)による触媒作用によりアルロ−ス6−リン酸(A6P)に変換すること;および
A6Pを、アルロ−ス6−リン酸ホスファタ−ゼ(A6PP)による触媒作用によりアルロ−スに変換すること、ここでA6PPは微生物細胞において発現される核酸配列によってコードされる、
を含む、前記方法。 - アルロ−スを生産する方法であって、方法が以下:
アルロ−ス6−リン酸エピメラ−ゼ(A6PE)、およびアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)を発現する微生物細胞からの細胞ライセートを含む細胞ライセート混合物において、フルクト−ス6−リン酸(F6P)を、A6PEによる触媒作用によりアルロ−ス6−リン酸(A6P)に変換すること、およびA6Pを、A6PPによる触媒作用によりアルロ−スに変換すること、ここでA6PEはBrevibacillus thermoruber核酸配列によってコードされている、前記方法。 - 細胞ライセート混合物において、多量体グルコ−ス炭水化物をアルロ−スに変換すること、ここで細胞ライセート混合物は、α−グルカンホスホリラ−ゼまたはセロデキストリンホスホリラ−ゼ、ホスホグルコムタ−ゼ、ホスホグルコイソメラ−ゼ、アルロ−ス6−リン酸エピメラ−ゼ(A6PE)、およびアルロ−ス6−リン酸ホスファタ−ゼ(A6PP)、ここでA6PEはBrevibacillus thermoruber核酸配列によってコードされる、を発現する微生物細胞からの細胞ライセートを含む、
を含む方法。 - 少なくとも1つの酵素が耐熱性であるか、または少なくとも2つの酵素が耐熱性である、請求項1〜9のいずれか一項に記載の方法。
- A6PE、A6PP、またはA6PEおよびA6PPの両方が耐熱性であるかまたは耐熱性であるように改変されている、請求項1〜10のいずれか一項に記載の方法。
- 多量体グルコ−ス炭水化物が、デンプン、セロデキストリン、マルトデキストリンまたはグリコ−ゲンから選択される、請求項1、2、10および11のいずれか一項に記載の方法。
- さらに多量体グルコ−ス炭水化物を枝切り酵素で処理することを含む、請求項1、2、および10〜12のいずれか一項に記載の方法。
- 枝切り酵素が、(i)イソアミラ−ゼ、任意にSulfolobus tokodaii、Metallosphaera hakonensis、Sphaerobacter thermophiles、およびBacillus lentusイソアミラ−ゼから選択される、および(ii)プルラナ−ゼ、任意にFervidobacterium pennavorans、Thermotoga sp. RQ5、Bacillus flavocaldarius、Thermosipho africanus、およびKosmotoga oleariaプルラナ−ゼから選択される、
から選択される、請求項13に記載の方法。 - A6PEが、Thermobacterium thermosaccharolyticum、Thermoanaerobacter brockii、Caldanaerobacter subterraneus、Deferribacter desulfuricans、Thermocrinis ruber、Hydrogenivirga sp. 128−5−R1−1、Brevibacillus thermoruber、Thermosipho atlanticus、およびThermosulfidibacter takaiiのアルロ−ス6−リン酸エピメラ−ゼからなる群から選択される、請求項1、4〜6、および10〜14のいずれか一項に記載の方法。
- A6PEが、Thermobacterium thermosaccharolyticum、Hydrogenivirga sp. 128−5−R1−1、およびBrevibacillus thermoruberからなる群から選択される、請求項15に記載の方法。
- α−グルカンホスホリラ−ゼが、Aquifex aeolicus、Thermocrinis minervae、 Thermosulfidibacter takaii、Thermosulfurimonas dismutans、Thermococcus litoralis、Palaeococcus pacificus、Thermotoga neapolitana、Ruminiclostridium thermocellum、Pyrococcus abyssi、Thermococcus thioreducens、Deinococcus radiodurans、Sulfolobus acidocaldarius、Thermus caldophilus、Meiothermus silvanus、Oceanithermus profundus、Ardenticatena maritima、Thermococcus barophilus、Pseudothermotoga thermarum、Hydrogenobacter thermophilus、Thermus oshimai、Meiothermus ruber、およびMarinitoga piezophilaのα−グルカンホスホリラ−ゼからなる群から選択される、請求項1、2、および10〜16のいずれか一項に記載の方法。
- セロデキストリンホスホリラ−ゼが、Clostridium thermocellum、Clostridium straminisolvens、Thermotoga RQ2、Ignisphaera aggregans、Thermotoga maritima、Spirochaeta thermophila、Caldicellulosiruptor bescii、Dictyoglomus thermophilum、Thermoanaerobacterium thermosaccharolyticum、Thermosipho africanus、Caldisalinibacter kiritimatiensis、Defluviitalea phaphyphila、Caldicellulosiruptor kronotskyensis、Thermococcus sibiricus、およびThermosphaera aggregansのセロデキストリンホスホリラ−ゼからなる群から選択される、請求項1、2、および10〜17のいずれか一項に記載の方法。
- ホスホグルコムタ−ゼが、Thermococcus kodakaraensis、Pyrococcus kukulkanii、Ammonifex degensii、Methanothermobacter wolfeii、Methanothermus fervidus、Sulfolobus acidocaldarius、Archaeoglobus fulgidus、Ferroglobus placidus、Geoglobus ahangari、Archaeoglobus veneficus、Archaeoglobus sulfaticallidus、Aciduliprofundum boonie、Clostridium thermocellum、Defluviitalea phaphyphila、Caminicella sporogenes、Caloranaerobacter ferrireducens、Thermosipho malanesiensis、Fervidobacterium pennivorans、Symbiobacterium thermophilum、Spirochaeta thermophila、およびThermoanaerobacter wiegeliiのホスホグルコムタ−ゼからなる群から選択される、請求項1、2、および10〜18のいずれか一項に記載の方法。
- ホスホグルコイソメラ−ゼが、Thermus thermophilus、Meiothermus timidus、Thermus filiformis、Marinithermus hydrothermalis、Thermosipho africanus、Sulfurihydrogenibium azorense、Persephonella marina、Marinitoga piezophila、Kosmotoga olearia、Thermotoga maritima、Geobacillus stearothermophilus、Anoxybacillus flavithermus、Thermosulfidibacter takaii、Fervidobacterium nodosum、Clostridium thermocellum、Thermoanaerobacterium thermosaccharolyticum、Methanococcus jannaschii、Methanotorris igneus、Methanocaldococcus villosus、Methanothermococcus okinawensis、Pseudothermotoga thermarum、Deferribacter desulfuricans、およびThermovibrio ammonificansのホスホグルコイソメラ−ゼからなる群から選択される、請求項1、2、および10〜19のいずれか一項に記載の方法。
- A6PPが、Thermoanaerobacter wiegelii、Thermoanaerobacter ethanolicus、Thermus islandicus、Deinococcus geothermalis DSM 11300、Thermosphaera aggregans、Crenarchaeota archaeon、Pyrococcus horikoshii Ot3、Aquifex aeolicus、Ruminiclostridium thermocellum、Desulfotomaculum kuznetsovii、Caldanaerobacter subterraneus、Acidothermus cellulolyticus、Methanothermobacter thermautotrophicus、Thermobifida fusca、Thermotoga neapolitana、Petrotoga mobilis、およびThermodesulfatator indicus、Thermus thermophilus、Bacteroides vulgatus、およびBacteroides fragilusのアルロ−ス6−リン酸ホスファタ−ゼからなる群から選択される、請求項2および7〜20のいずれかに記載の方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762443447P | 2017-01-06 | 2017-01-06 | |
US62/443,447 | 2017-01-06 | ||
US201762538181P | 2017-07-28 | 2017-07-28 | |
US62/538,181 | 2017-07-28 | ||
PCT/US2018/012516 WO2018129275A1 (en) | 2017-01-06 | 2018-01-05 | Cell-free production of sugars |
Publications (3)
Publication Number | Publication Date |
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JP2020503059A JP2020503059A (ja) | 2020-01-30 |
JP2020503059A5 true JP2020503059A5 (ja) | 2021-05-20 |
JP7186167B2 JP7186167B2 (ja) | 2022-12-08 |
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Kim et al. | ted | |
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