JP2020501612A5 - - Google Patents
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- JP2020501612A5 JP2020501612A5 JP2019554474A JP2019554474A JP2020501612A5 JP 2020501612 A5 JP2020501612 A5 JP 2020501612A5 JP 2019554474 A JP2019554474 A JP 2019554474A JP 2019554474 A JP2019554474 A JP 2019554474A JP 2020501612 A5 JP2020501612 A5 JP 2020501612A5
- Authority
- JP
- Japan
- Prior art keywords
- transposase
- seq
- cells
- stem cells
- fusion
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 102000008579 Transposases Human genes 0.000 claims description 152
- 108010020764 Transposases Proteins 0.000 claims description 152
- 150000001413 amino acids Chemical group 0.000 claims description 66
- 230000004927 fusion Effects 0.000 claims description 62
- 235000001014 amino acid Nutrition 0.000 claims description 57
- 238000006467 substitution reaction Methods 0.000 claims description 53
- 238000000034 method Methods 0.000 claims description 52
- 210000004027 cell Anatomy 0.000 claims description 49
- 108091033319 polynucleotide Proteins 0.000 claims description 24
- 102000040430 polynucleotide Human genes 0.000 claims description 24
- 239000002157 polynucleotide Substances 0.000 claims description 24
- 108020004999 messenger RNA Proteins 0.000 claims description 14
- 108020004414 DNA Proteins 0.000 claims description 13
- 210000000130 stem cell Anatomy 0.000 claims description 11
- 230000007935 neutral effect Effects 0.000 claims description 10
- 108700019146 Transgenes Proteins 0.000 claims description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 8
- 235000018102 proteins Nutrition 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 230000009870 specific binding Effects 0.000 claims description 7
- 239000013598 vector Substances 0.000 claims description 7
- 239000004475 Arginine Substances 0.000 claims description 6
- 108091008875 B cell receptors Proteins 0.000 claims description 6
- 108010019670 Chimeric Antigen Receptors Proteins 0.000 claims description 6
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims description 6
- 239000004472 Lysine Substances 0.000 claims description 6
- 108091008874 T cell receptors Proteins 0.000 claims description 6
- 102000016266 T-Cell Antigen Receptors Human genes 0.000 claims description 6
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 6
- 239000013612 plasmid Substances 0.000 claims description 5
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims description 4
- 238000001890 transfection Methods 0.000 claims description 4
- 230000017105 transposition Effects 0.000 claims description 4
- 102000004127 Cytokines Human genes 0.000 claims description 3
- 108090000695 Cytokines Proteins 0.000 claims description 3
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims description 3
- 102000001749 Immunologic Receptors Human genes 0.000 claims description 3
- 108010054738 Immunologic Receptors Proteins 0.000 claims description 3
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 3
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 claims description 3
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 claims description 3
- 102100029812 Protein S100-A12 Human genes 0.000 claims description 3
- 101710110949 Protein S100-A12 Proteins 0.000 claims description 3
- 235000003704 aspartic acid Nutrition 0.000 claims description 3
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 3
- 230000003197 catalytic effect Effects 0.000 claims description 3
- 235000013922 glutamic acid Nutrition 0.000 claims description 3
- 239000004220 glutamic acid Substances 0.000 claims description 3
- 238000006384 oligomerization reaction Methods 0.000 claims description 3
- 210000004986 primary T-cell Anatomy 0.000 claims description 3
- 108020003175 receptors Proteins 0.000 claims description 3
- 102000005962 receptors Human genes 0.000 claims description 3
- 102220036871 rs587780095 Human genes 0.000 claims description 3
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 claims description 2
- 210000004504 adult stem cell Anatomy 0.000 claims description 2
- 238000003556 assay Methods 0.000 claims description 2
- 210000004369 blood Anatomy 0.000 claims description 2
- 239000008280 blood Substances 0.000 claims description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 2
- 239000001506 calcium phosphate Substances 0.000 claims description 2
- 235000011010 calcium phosphates Nutrition 0.000 claims description 2
- 229920006317 cationic polymer Polymers 0.000 claims description 2
- 239000000412 dendrimer Substances 0.000 claims description 2
- 229920000736 dendritic polymer Polymers 0.000 claims description 2
- 210000003162 effector t lymphocyte Anatomy 0.000 claims description 2
- 238000004520 electroporation Methods 0.000 claims description 2
- 210000001671 embryonic stem cell Anatomy 0.000 claims description 2
- 230000003511 endothelial effect Effects 0.000 claims description 2
- 210000002514 epidermal stem cell Anatomy 0.000 claims description 2
- 210000004475 gamma-delta t lymphocyte Anatomy 0.000 claims description 2
- 210000002443 helper t lymphocyte Anatomy 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 210000004966 intestinal stem cell Anatomy 0.000 claims description 2
- 210000000265 leukocyte Anatomy 0.000 claims description 2
- 239000002502 liposome Substances 0.000 claims description 2
- 210000004216 mammary stem cell Anatomy 0.000 claims description 2
- 210000003071 memory t lymphocyte Anatomy 0.000 claims description 2
- 210000002901 mesenchymal stem cell Anatomy 0.000 claims description 2
- 238000000520 microinjection Methods 0.000 claims description 2
- 210000000581 natural killer T-cell Anatomy 0.000 claims description 2
- 230000001537 neural effect Effects 0.000 claims description 2
- 210000001178 neural stem cell Anatomy 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims description 2
- 238000001556 precipitation Methods 0.000 claims description 2
- 210000001938 protoplast Anatomy 0.000 claims description 2
- 210000001550 testis Anatomy 0.000 claims description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 claims description 2
- 239000011701 zinc Substances 0.000 claims description 2
- 229910052725 zinc Inorganic materials 0.000 claims description 2
- 230000027455 binding Effects 0.000 claims 2
- 210000002865 immune cell Anatomy 0.000 claims 1
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 1
- 206010028980 Neoplasm Diseases 0.000 description 4
- 230000004568 DNA-binding Effects 0.000 description 3
- 201000011510 cancer Diseases 0.000 description 2
- 238000010362 genome editing Methods 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004990 primary immune cell Anatomy 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 150000007523 nucleic acids Chemical group 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662435522P | 2016-12-16 | 2016-12-16 | |
| US62/435,522 | 2016-12-16 | ||
| PCT/US2017/066829 WO2018112415A1 (en) | 2016-12-16 | 2017-12-15 | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2020501612A JP2020501612A (ja) | 2020-01-23 |
| JP2020501612A5 true JP2020501612A5 (https=) | 2021-02-04 |
| JP7275043B2 JP7275043B2 (ja) | 2023-05-17 |
Family
ID=62559537
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2019554474A Active JP7275043B2 (ja) | 2016-12-16 | 2017-12-15 | 増大したｈＡＴファミリートランスポゾン媒介遺伝子導入ならびに関連する組成物、システムおよび方法 |
Country Status (10)
| Country | Link |
|---|---|
| US (5) | US11111483B2 (https=) |
| EP (2) | EP3555273B1 (https=) |
| JP (1) | JP7275043B2 (https=) |
| KR (1) | KR102523318B1 (https=) |
| CN (1) | CN110730821B (https=) |
| AU (1) | AU2017378482B2 (https=) |
| CA (1) | CA3047313A1 (https=) |
| EA (1) | EA201991431A1 (https=) |
| IL (1) | IL267334B2 (https=) |
| WO (1) | WO2018112415A1 (https=) |
Families Citing this family (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| AU2017378482B2 (en) | 2016-12-16 | 2024-06-13 | R&D Systems, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| AU2019288669B2 (en) * | 2018-06-21 | 2025-06-26 | R&D Systems, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| CN115698268A (zh) * | 2020-05-04 | 2023-02-03 | 萨利欧基因治疗公司 | 基于转座的疗法 |
| US20230272355A1 (en) * | 2020-06-05 | 2023-08-31 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY MEMBER SPIN TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
| EP4165180A4 (en) | 2020-06-16 | 2024-10-23 | Bio-Techne Corporation | MODIFIED MAD7-DIRECTED ENDONUCLEASE |
| CN111607661B (zh) * | 2020-06-29 | 2022-06-14 | 北部湾大学 | 基于油茶转录组hAT转座子的分子标记引物组及其应用 |
| CN111607662B (zh) * | 2020-06-30 | 2022-06-10 | 北部湾大学 | 基于白骨壤转录组hAT转座子的分子标记引物组及其应用 |
| WO2023282730A1 (ko) * | 2021-07-09 | 2023-01-12 | 주식회사 네오젠티씨 | 트랜스포존 시스템 및 이의 용도 |
| WO2023004366A1 (en) | 2021-07-21 | 2023-01-26 | Aspen Neuroscience, Inc. | Transposon-based modulation of gba1 and related compositions and uses thereof |
| WO2023172514A1 (en) | 2022-03-07 | 2023-09-14 | Catamaran Bio, Inc. | Engineered immune cell therapeutics targeted to her2 and methods of use thereof |
| JP2025515603A (ja) | 2022-04-28 | 2025-05-20 | イマティクス ユーエス，アイエヌシー． | Ｉｌ－１２ポリペプチド、ｉｌ－１５ポリペプチド、ｉｌ－１８ポリペプチド、ｃｄ８ポリペプチド、それらの組成物、およびそれらの使用方法 |
| JP2025515604A (ja) | 2022-04-28 | 2025-05-20 | イマティクス ユーエス，アイエヌシー． | ドミナントネガティブＴＧＦβ受容体ポリペプチド、ＣＤ８ポリペプチド、細胞、組成物、およびその使用方法 |
| JP2025516189A (ja) | 2022-04-28 | 2025-05-27 | イマティクス ユーエス，アイエヌシー． | 膜結合型ｉｌ－１５、ｃｄ８ポリペプチド、細胞、組成物、およびそれらの使用方法 |
| WO2025054409A1 (en) * | 2023-09-07 | 2025-03-13 | Bio-Techne Corporation | Mutant tcbuster transposase with improved solubility |
| WO2025096649A1 (en) | 2023-11-01 | 2025-05-08 | Immatics US, Inc. | Membrane-bound il-15, cd8 polypeptides, cells, compositions, and methods of using thereof |
| WO2025156492A1 (zh) * | 2024-01-23 | 2025-07-31 | 星尘股份有限公司 | 转座酶及其用途 |
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| EP1458875A2 (de) | 2001-12-20 | 2004-09-22 | Sungene GmbH & Co. KGaA | Verfahren zur transformation von pflanzlichen plastiden |
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-
2017
- 2017-12-15 AU AU2017378482A patent/AU2017378482B2/en active Active
- 2017-12-15 EP EP17880424.1A patent/EP3555273B1/en active Active
- 2017-12-15 CA CA3047313A patent/CA3047313A1/en active Pending
- 2017-12-15 JP JP2019554474A patent/JP7275043B2/ja active Active
- 2017-12-15 KR KR1020197020507A patent/KR102523318B1/ko active Active
- 2017-12-15 US US15/844,363 patent/US11111483B2/en active Active
- 2017-12-15 EA EA201991431A patent/EA201991431A1/ru unknown
- 2017-12-15 WO PCT/US2017/066829 patent/WO2018112415A1/en not_active Ceased
- 2017-12-15 IL IL267334A patent/IL267334B2/en unknown
- 2017-12-15 EP EP24170568.0A patent/EP4421167A3/en active Pending
- 2017-12-15 CN CN201780086768.7A patent/CN110730821B/zh active Active
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2018
- 2018-04-20 US US15/958,834 patent/US20180245055A1/en not_active Abandoned
- 2018-06-11 US US16/005,270 patent/US10227574B2/en active Active
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2020
- 2020-02-12 US US16/789,188 patent/US11162084B2/en active Active
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2021
- 2021-10-22 US US17/508,522 patent/US12037611B2/en active Active
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