JP2019528726A5 - - Google Patents

Download PDF

Info

Publication number
JP2019528726A5
JP2019528726A5 JP2019514212A JP2019514212A JP2019528726A5 JP 2019528726 A5 JP2019528726 A5 JP 2019528726A5 JP 2019514212 A JP2019514212 A JP 2019514212A JP 2019514212 A JP2019514212 A JP 2019514212A JP 2019528726 A5 JP2019528726 A5 JP 2019528726A5
Authority
JP
Japan
Prior art keywords
nucleic acid
oligonucleotide
moiety
quenching
tag portion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2019514212A
Other languages
English (en)
Japanese (ja)
Other versions
JP2019528726A (ja
JP7063886B2 (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/EP2017/073293 external-priority patent/WO2018050828A1/en
Publication of JP2019528726A publication Critical patent/JP2019528726A/ja
Publication of JP2019528726A5 publication Critical patent/JP2019528726A5/ja
Application granted granted Critical
Publication of JP7063886B2 publication Critical patent/JP7063886B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2019514212A 2016-09-15 2017-09-15 マルチプレックスリアルタイムｐｃｒを実施する方法 Active JP7063886B2 (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662395325P 2016-09-15 2016-09-15
US62/395,325 2016-09-15
US201662435595P 2016-12-16 2016-12-16
US62/435,595 2016-12-16
US201762536871P 2017-07-25 2017-07-25
US62/536,871 2017-07-25
PCT/EP2017/073293 WO2018050828A1 (en) 2016-09-15 2017-09-15 Methods for performing multiplexed real-time pcr

Publications (3)

Publication Number Publication Date
JP2019528726A JP2019528726A (ja) 2019-10-17
JP2019528726A5 true JP2019528726A5 (cg-RX-API-DMAC10.html) 2020-10-22
JP7063886B2 JP7063886B2 (ja) 2022-05-09

Family

ID=59901522

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2019514212A Active JP7063886B2 (ja) 2016-09-15 2017-09-15 マルチプレックスリアルタイムｐｃｒを実施する方法
JP2019514294A Active JP7221199B2 (ja) 2016-09-15 2017-09-15 マルチプレックスｐｃｒの実施方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2019514294A Active JP7221199B2 (ja) 2016-09-15 2017-09-15 マルチプレックスｐｃｒの実施方法

Country Status (10)

Country Link
US (3) US11028433B2 (cg-RX-API-DMAC10.html)
EP (2) EP3512959B1 (cg-RX-API-DMAC10.html)
JP (2) JP7063886B2 (cg-RX-API-DMAC10.html)
KR (2) KR102468633B1 (cg-RX-API-DMAC10.html)
CN (5) CN109689884A (cg-RX-API-DMAC10.html)
AU (2) AU2017328613B2 (cg-RX-API-DMAC10.html)
CA (2) CA3033250C (cg-RX-API-DMAC10.html)
DK (1) DK3512959T3 (cg-RX-API-DMAC10.html)
ES (2) ES2893411T3 (cg-RX-API-DMAC10.html)
WO (2) WO2018050824A1 (cg-RX-API-DMAC10.html)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK3512959T3 (da) 2016-09-15 2021-10-18 Hoffmann La Roche Fremgangsmåder til udførelse af multipleks PCR
RU2665631C2 (ru) * 2017-01-25 2018-09-03 Общество с ограниченной ответственностью "Секвойя Дженетикс" Способ специфической идентификации последовательностей днк
US20190093155A1 (en) * 2017-05-25 2019-03-28 Roche Molecular Systems, Inc. Multiplex Nucleic Acid Amplification Assay
KR20200115552A (ko) 2018-01-22 2020-10-07 루미넥스 코포레이션 별개의 용융 분석을 위한 방법 및 조성물
US20190292596A1 (en) 2018-03-21 2019-09-26 Roche Molecular Systems, Inc. Modified nucleoside phosphates with high thermal stability
IL277870B2 (en) 2018-04-20 2025-02-01 Seegene Inc Method and device for identifying diverse targets of nucleic acid sequences in a sample
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
SG11202101140TA (en) 2018-08-09 2021-03-30 Speedx Pty Ltd Multiplex detection of nucleic acids
US11371080B2 (en) 2018-11-30 2022-06-28 Luminex Corporation Methods and probes for performing PCR with melt analysis for increased multiplexing
EP3899035B1 (en) * 2018-12-20 2025-04-16 F. Hoffmann-La Roche AG Detection of target nucleic acid by solid-phase molography
EP4031683A4 (en) 2019-09-16 2024-01-17 Exact Sciences Corporation Structure and temperature-dependent flap endonuclease substrates
US11441167B2 (en) 2019-11-20 2022-09-13 Roche Molecular Systems, Inc. Compositions and methods for rapid identification and phenotypic antimicrobial susceptibility testing of bacteria and fungi
JP7794737B2 (ja) 2019-12-09 2026-01-06 エフ． ホフマン－ラ ロシュ アーゲー ジカチオン性蛍光色素
CN111534518B (zh) * 2020-05-18 2021-07-23 纳昂达(南京)生物科技有限公司 通用封闭序列及其应用
CN116018414A (zh) * 2020-06-30 2023-04-25 斯皮德斯私人有限公司 使用混合报告子的多重核酸检测
CN112195221B (zh) * 2020-09-13 2021-08-06 武汉纳诺德医疗科技有限公司 一种多温度单荧光通道多重实时荧光定量pcr仪及其检测方法
EP4592401A3 (en) * 2020-09-16 2025-10-29 Bruker Spatial Biology, Inc. Chemical compositions and methods of using the same
EP4267764A1 (en) 2020-12-22 2023-11-01 F. Hoffmann-La Roche AG Methods for performing multiplexed real-time pcr with the use of large stokes shift fluorescent dyes
JP2024522640A (ja) * 2021-06-25 2024-06-21 エフ． ホフマン－ラ ロシュ アーゲー 高感度で温度多重化ｐｃｒを行うための方法
CA3232383C (en) 2021-09-30 2026-05-12 Gen-Probe Incorporated Temperature-selectable fret cassette signaling
CN119487130A (zh) 2022-06-28 2025-02-18 豪夫迈·罗氏有限公司 具有大斯托克斯位移的荧光染料
EP4621068A1 (en) * 2022-11-16 2025-09-24 Seegene, Inc. Detection of target nucleic acid by quenching-pto cleavage and extension (q-ptoce) assay
AR132405A1 (es) * 2023-04-19 2025-06-25 Consejo Nacional De Investigaciones Cientificas Y Tecn Conicet Métodos y sistemas para seleccionar biomarcadores basados en metilación del adn para la detección de cáncer colorrectal
KR20260034624A (ko) * 2023-05-15 2026-03-11 스피디엑스 피티와이 리미티드 온도 제어 프로브를 이용한 표적 검출
US20250062438A1 (en) * 2023-08-18 2025-02-20 Valeo Systemes Thermiques Heat exchanger plate for battery pack
KR20260035911A (ko) * 2023-09-08 2026-03-13 주식회사 씨젠 3개의 프로브를 이용하여 타겟 핵산을 검출하는 방법
WO2025141105A1 (en) 2023-12-27 2025-07-03 bioMérieux Specific probes for the detection of nucleic acids, methods and uses
EP4606908A1 (en) * 2024-02-21 2025-08-27 Roche Diagnostics GmbH Method of increasing the number of usable channels in multiplex pcr
WO2026052826A1 (en) 2024-09-09 2026-03-12 Roche Diagnostics Gmbh Methods for producing fluorescent dyes
WO2026125666A1 (en) 2024-12-11 2026-06-18 Roche Molecular Systems, Inc. Methods for detection of arthropod-borne viruses by multiplex pcr

Family Cites Families (61)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4250257A (en) * 1978-08-24 1981-02-10 Technicon Instruments Corporation Whole blood analyses in porous media
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5455170A (en) 1986-08-22 1995-10-03 Hoffmann-La Roche Inc. Mutated thermostable nucleic acid polymerase enzyme from Thermus species Z05
US5618711A (en) 1986-08-22 1997-04-08 Hoffmann-La Roche Inc. Recombinant expression vectors and purification methods for Thermus thermophilus DNA polymerase
US5405774A (en) 1986-08-22 1995-04-11 Hoffmann-La Roche Inc. DNA encoding a mutated thermostable nucleic acid polymerase enzyme from thermus species sps17
US5352600A (en) 1986-08-22 1994-10-04 Hoffmann-La Roche Inc. Purified thermostable enzyme
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5079352A (en) 1986-08-22 1992-01-07 Cetus Corporation Purified thermostable enzyme
US5795762A (en) 1986-08-22 1998-08-18 Roche Molecular Systems, Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
JP2709311B2 (ja) 1990-09-28 1998-02-04 エフ．ホフマン−ラ ロシュ アクチェンゲゼルシャフト 熱安定性ｄｎａポリメラーゼの５→３′のエキソヌクレアーゼ突然変異
US5539082A (en) 1993-04-26 1996-07-23 Nielsen; Peter E. Peptide nucleic acids
DE4234086A1 (de) 1992-02-05 1993-08-12 Diagen Inst Molekularbio Verfahren zur bestimmung von in vitro amplifizierten nukleinsaeuresequenzen
US5541311A (en) 1992-12-07 1996-07-30 Third Wave Technologies, Inc. Nucleic acid encoding synthesis-deficient thermostable DNA polymerase
US5843691A (en) * 1993-05-15 1998-12-01 Lifescan, Inc. Visually-readable reagent test strip
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5571673A (en) 1994-11-23 1996-11-05 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
AU7236296A (en) 1995-09-08 1997-03-27 Life Technologies, Inc. Cloned dna polymerases from thermotoga and mutants thereof
DE69739357D1 (de) 1996-06-04 2009-05-28 Univ Utah Res Found Überwachung der hybridisierung während pcr
US6427156B1 (en) 1997-01-21 2002-07-30 Xilinx, Inc. Configurable logic block with AND gate for efficient multiplication in FPGAS
JP3756313B2 (ja) 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6794499B2 (en) 1997-09-12 2004-09-21 Exiqon A/S Oligonucleotide analogues
US6472156B1 (en) 1999-08-30 2002-10-29 The University Of Utah Homogeneous multiplex hybridization analysis by color and Tm
US6893819B1 (en) 2000-11-21 2005-05-17 Stratagene California Methods for detection of a nucleic acid by sequential amplification
US7585632B2 (en) * 1999-10-29 2009-09-08 Hologic, Inc. Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US7381532B2 (en) 1999-10-29 2008-06-03 Stratagene California Compositions and methods for the detection of a nucleic acid using a cleavage reaction
US20050053939A1 (en) 2001-11-09 2005-03-10 Ahmed Chenna Methods and compositions for enhancing detection in determinations employing cleavable electrophoretic tag reagents
EP3483599A1 (en) * 2005-07-20 2019-05-15 Ascensia Diabetes Care Holdings AG Methods for measuring the concentration of an analyte in solution and for calibrating the measurement as well as handheld devices therefor
US7833716B2 (en) * 2006-06-06 2010-11-16 Gen-Probe Incorporated Tagged oligonucleotides and their use in nucleic acid amplification methods
WO2008021446A2 (en) * 2006-08-15 2008-02-21 Genetag Technology, Inc. Probe-antiprobe compositions and methods for dna or rna detection
EP2078189B1 (en) * 2006-10-20 2012-10-10 Clondiag GmbH Assay devices and methods for the detection of analytes
US8133701B2 (en) 2006-12-05 2012-03-13 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
US7902345B2 (en) 2006-12-05 2011-03-08 Sequenom, Inc. Detection and quantification of biomolecules using mass spectrometry
EP2118310B1 (en) 2006-12-29 2013-03-06 Applied Biosystems, LLC Systems and methods for detecting nucleic acids
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
US10150990B2 (en) 2008-04-21 2018-12-11 Roche Molecular Systems, Inc. Ribonucleotide tag nucleic acid detection
GB0909333D0 (en) * 2009-06-01 2009-07-15 Fu Guoliang Multiplex amplification and detection
US9234232B2 (en) * 2008-07-31 2016-01-12 Oxitec Limited Multiplex amplification and detection
US20120252015A1 (en) * 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
KR101073875B1 (ko) * 2008-11-28 2011-10-14 한국생명공학연구원 대장암 진단 마커 및 이를 이용한 대장암 진단방법
US8385895B2 (en) 2008-12-18 2013-02-26 Verizon Patent And Licensing Inc. Updating an operating parameter
US8039215B2 (en) 2009-03-10 2011-10-18 Roche Molecular Systems, Inc. Multiplex quantitative nucleic acid amplification and melting assay
KR101177320B1 (ko) * 2010-02-26 2012-09-10 문우철 Ｙ형 프로브 및 이것의 변형형, 및 이를 이용한 ｄｎａ 마이크로어레이, 키트 및 유전자 분석방법
US8361720B2 (en) * 2010-11-15 2013-01-29 Exact Sciences Corporation Real time cleavage assay
MX352460B (es) 2011-01-11 2017-11-24 Seegene Inc Detección de secuencias de ácido nucleico objetivo mediante ensayo de escisión y extensión del pto.
KR20130101952A (ko) * 2012-02-02 2013-09-16 주식회사 씨젠 Ｐｔｏ 절단과 연장-의존적 혼성화를 이용한 타겟 핵산서열의 검출
US10106842B2 (en) 2013-03-15 2018-10-23 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
US9828629B2 (en) * 2013-03-15 2017-11-28 Roche Molecular Systems, Inc. Nucleic acid target identification by structure based probe cleavage
EP3521452B1 (en) 2013-06-19 2021-08-04 Luminex Corporation Real-time multiplexed hydrolysis probe assay
US9637791B2 (en) 2013-12-20 2017-05-02 Roche Molecular Systems, Inc. Multiplexed nucleic acid target identification by strucutre based probe cleavage
WO2015147370A1 (en) 2014-03-28 2015-10-01 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures
EP3180449B1 (en) * 2014-08-11 2019-10-09 Luminex Corporation Probes for improved melt discrimination and multiplexing in nucleic acid assays
WO2016093620A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
WO2016093619A1 (en) 2014-12-09 2016-06-16 Seegene, Inc. Detection of target nucleic acid sequences using different detection temperatures and reference values
CN107109492B (zh) * 2014-12-22 2021-02-26 阿纳帕生物技术股份公司 用于靶核酸多重检测的双重猝灭测定
DK3512959T3 (da) 2016-09-15 2021-10-18 Hoffmann La Roche Fremgangsmåder til udførelse af multipleks PCR
US10590469B2 (en) * 2016-09-15 2020-03-17 Roche Molecular Svstems, Inc. Methods for performing multiplexed real-time PCR in a self-contained nucleic acid analysis pouch

Similar Documents

Publication Publication Date Title
JP2019532628A5 (cg-RX-API-DMAC10.html)
US12534754B2 (en) Attenuators
US11293048B2 (en) Attenuators
EP2756101B1 (en) Probe: antiprobe compositions for high specificity dna or rna detection
JP2010519896A5 (cg-RX-API-DMAC10.html)
JP2012245004A5 (cg-RX-API-DMAC10.html)
RU2012109893A (ru) Зонд td и его применения
RU2012129362A (ru) Обнаружение мишени tsg праймером
RU2012142160A (ru) Детекция нуклеиновокислотных последовательностей-мишеней в анализе с расщеплением и удлинением рто
JP2019528726A (ja) マルチプレックスリアルタイムｐｃｒを実施する方法
JP2010519897A5 (cg-RX-API-DMAC10.html)
JP2002502615A5 (cg-RX-API-DMAC10.html)
JP2005506075A5 (cg-RX-API-DMAC10.html)
JP5851395B2 (ja) 食中毒菌検出用担体、及び食中毒菌の検出方法
JP2013509871A5 (cg-RX-API-DMAC10.html)
KR102397357B1 (ko) 포스포로티오에이트 dna로 수식된 헤어핀 프로브 기반의 등온 핵산증폭기술을 이용한 표적핵산 검출방법
US20200032310A1 (en) Mispriming prevention reagents
JPWO2012173274A1 (ja) 核酸測定用の核酸プローブ
US10801056B2 (en) Kit and method for detection of microRNA
CN102321765B (zh) 一种实时荧光pcr方法及用途
JP2016527904A5 (cg-RX-API-DMAC10.html)
JP2022533088A5 (cg-RX-API-DMAC10.html)
CN108642165A (zh) 一种用于实时荧光pcr的探针及其使用方法
JP2022545280A5 (cg-RX-API-DMAC10.html)
JP2019502374A5 (cg-RX-API-DMAC10.html)