JP2019526225A5 - - Google Patents

Info

Publication number

JP2019526225A5

JP2019526225A5 JP2018556287A JP2018556287A JP2019526225A5 JP 2019526225 A5 JP2019526225 A5 JP 2019526225A5 JP 2018556287 A JP2018556287 A JP 2018556287A JP 2018556287 A JP2018556287 A JP 2018556287A JP 2019526225 A5 JP2019526225 A5 JP 2019526225A5

Authority

JP

Japan

Prior art keywords

dna polymerase

seq

primer

nucleotide

kit

Prior art date

2017-04-28

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Granted

Links

• Espacenet
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• 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims 37
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims 37
• 239000002773 nucleotide Substances 0.000 claims 30
• 125000003729 nucleotide group Chemical group 0.000 claims 30
• 238000000034 method Methods 0.000 claims 18
• 108020004707 nucleic acids Proteins 0.000 claims 16
• 102000039446 nucleic acids Human genes 0.000 claims 16
• 150000007523 nucleic acids Chemical class 0.000 claims 16
• 230000002950 deficient Effects 0.000 claims 15
• 229920001184 polypeptide Polymers 0.000 claims 15
• 102000004196 processed proteins & peptides Human genes 0.000 claims 15
• 108090000765 processed proteins & peptides Proteins 0.000 claims 15
• WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 claims 14
• 125000003275 alpha amino acid group Chemical group 0.000 claims 14
• 235000001014 amino acid Nutrition 0.000 claims 14
• 235000013922 glutamic acid Nutrition 0.000 claims 14
• 239000004220 glutamic acid Substances 0.000 claims 14
• 125000000291 glutamic acid group Chemical group N[C@@H](CCC(O)=O)C(=O)* 0.000 claims 14
• 230000002441 reversible effect Effects 0.000 claims 12
• 239000011541 reaction mixture Substances 0.000 claims 6
• FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical group C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 4
• 239000005547 deoxyribonucleotide Substances 0.000 claims 4
• 239000011777 magnesium Substances 0.000 claims 4
• 229910001437 manganese ion Inorganic materials 0.000 claims 4
• 235000011178 triphosphate Nutrition 0.000 claims 4
• 239000001226 triphosphate Substances 0.000 claims 4
• FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 3
• 125000002637 deoxyribonucleotide group Chemical group 0.000 claims 3
• 229910052749 magnesium Inorganic materials 0.000 claims 3
• UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 claims 3
• AHCYMLUZIRLXAA-SHYZEUOFSA-N Deoxyuridine 5'-triphosphate Chemical compound O1[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C[C@@H]1N1C(=O)NC(=O)C=C1 AHCYMLUZIRLXAA-SHYZEUOFSA-N 0.000 claims 2
• 230000015572 biosynthetic process Effects 0.000 claims 2
• 238000006664 bond formation reaction Methods 0.000 claims 2
• 239000003054 catalyst Substances 0.000 claims 2
• SUYVUBYJARFZHO-RRKCRQDMSA-N dATP Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-RRKCRQDMSA-N 0.000 claims 2
• SUYVUBYJARFZHO-UHFFFAOYSA-N dATP Natural products C1=NC=2C(N)=NC=NC=2N1C1CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 SUYVUBYJARFZHO-UHFFFAOYSA-N 0.000 claims 2
• RGWHQCVHVJXOKC-SHYZEUOFSA-J dCTP(4-) Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)C1 RGWHQCVHVJXOKC-SHYZEUOFSA-J 0.000 claims 2
• HAAZLUGHYHWQIW-KVQBGUIXSA-N dGTP Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@H]1C[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O1 HAAZLUGHYHWQIW-KVQBGUIXSA-N 0.000 claims 2
• NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims 2
• 125000002887 hydroxy group Chemical group [H]O* 0.000 claims 2
• 230000035772 mutation Effects 0.000 claims 2
• JLVVSXFLKOJNIY-UHFFFAOYSA-N Magnesium ion Chemical compound [Mg+2] JLVVSXFLKOJNIY-UHFFFAOYSA-N 0.000 claims 1
• 239000003153 chemical reaction reagent Substances 0.000 claims 1
• 230000000295 complement effect Effects 0.000 claims 1
• -1 deoxyribonucleotide triphosphates Chemical class 0.000 claims 1
• 230000001419 dependent effect Effects 0.000 claims 1
• 230000000694 effects Effects 0.000 claims 1
• 238000010348 incorporation Methods 0.000 claims 1
• 150000002500 ions Chemical class 0.000 claims 1
• OOYGSFOGFJDDHP-KMCOLRRFSA-N kanamycin A sulfate Chemical group OS(O)(=O)=O.O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N OOYGSFOGFJDDHP-KMCOLRRFSA-N 0.000 claims 1
• 229910001425 magnesium ion Inorganic materials 0.000 claims 1