KR102231977B1 - 핵산 서열의 결정 방법 - Google Patents

핵산 서열의 결정 방법 Download PDF

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KR102231977B1
KR102231977B1 KR1020187034503A KR20187034503A KR102231977B1 KR 102231977 B1 KR102231977 B1 KR 102231977B1 KR 1020187034503 A KR1020187034503 A KR 1020187034503A KR 20187034503 A KR20187034503 A KR 20187034503A KR 102231977 B1 KR102231977 B1 KR 102231977B1
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nucleotide
nucleic acid
polymerase
dna polymerase
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KR20190003660A (ko
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칸다스와미 비자얀
피나르 이위도간
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옴니옴 인코포레이티드
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/518—Detection characterised by immobilisation to a surface characterised by the immobilisation of the nucleic acid sample or target
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)
KR1020187034503A 2016-04-29 2017-04-28 핵산 서열의 결정 방법 Active KR102231977B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201662329489P 2016-04-29 2016-04-29
US62/329,489 2016-04-29
US201762444733P 2017-01-10 2017-01-10
US62/444,733 2017-01-10
PCT/US2017/030135 WO2017190012A1 (en) 2016-04-29 2017-04-28 Method of nucleic acid sequence determination

Publications (2)

Publication Number Publication Date
KR20190003660A KR20190003660A (ko) 2019-01-09
KR102231977B1 true KR102231977B1 (ko) 2021-03-24

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KR1020187034503A Active KR102231977B1 (ko) 2016-04-29 2017-04-28 핵산 서열의 결정 방법

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US (2) US10584379B2 (enExample)
EP (1) EP3449011B1 (enExample)
JP (1) JP6915939B2 (enExample)
KR (1) KR102231977B1 (enExample)
CN (1) CN109154024B (enExample)
AU (1) AU2017258523B2 (enExample)
CA (1) CA3022431C (enExample)
SG (1) SG11201809522WA (enExample)
WO (1) WO2017190012A1 (enExample)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109154024B (zh) 2016-04-29 2022-03-04 欧姆尼欧美公司 核酸序列测定方法
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
EP4039824A1 (en) * 2016-08-15 2022-08-10 Pacific Biosciences of California, Inc. Method and system for sequencing nucleic acids
CA3050695C (en) 2017-01-20 2024-02-20 Omniome, Inc. Process for cognate nucleotide detection in a nucleic acid sequencing workflow
US10161003B2 (en) 2017-04-25 2018-12-25 Omniome, Inc. Methods and apparatus that increase sequencing-by-binding efficiency
EP3697932A1 (en) 2017-10-19 2020-08-26 Omniome, Inc. Simultaneous background reduction and complex stabilization in binding assay workflows
CN111164222A (zh) 2018-02-16 2020-05-15 伊鲁米纳公司 带有电荷标签的核苷酸及其使用方法
EP3784800B1 (en) 2018-04-26 2023-05-03 Pacific Biosciences of California, Inc. Methods and compositions for stabilizing nucleic acid-nucleotide-polymerase complexes
CN112384296B (zh) 2018-05-17 2023-06-27 识别分析股份有限公司 用于直接电测量酶活性的装置、系统和方法
EP3802874A1 (en) 2018-05-31 2021-04-14 Omniome, Inc. Increased signal to noise in nucleic acid sequencing
EP3827097A1 (en) 2018-07-24 2021-06-02 Omniome, Inc. Serial formation of ternary complex species
EP3853358A1 (en) 2018-09-17 2021-07-28 Omniome, Inc. Engineered polymerases for improved sequencing
US10876148B2 (en) 2018-11-14 2020-12-29 Element Biosciences, Inc. De novo surface preparation and uses thereof
US10768173B1 (en) 2019-09-06 2020-09-08 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
US10704094B1 (en) 2018-11-14 2020-07-07 Element Biosciences, Inc. Multipart reagents having increased avidity for polymerase binding
WO2020101795A1 (en) 2018-11-15 2020-05-22 Omniome, Inc. Electronic detection of nucleic acid structure
CN113166805B (zh) 2018-12-04 2024-07-16 加利福尼亚太平洋生物科学股份有限公司 用于核酸测序和其他分析测定的混合相流体
EP3899032A2 (en) 2018-12-20 2021-10-27 Omniome, Inc. Temperature control for analysis of nucleic acids and other analytes
MX2021009150A (es) 2019-01-30 2021-12-10 Univ Arizona State Circuitos bioelectronicos, sistemas y metodos para preparar y usar los mismos.
EP3924513B1 (en) 2019-02-14 2023-04-12 Pacific Biosciences of California, Inc. Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes
US12313627B2 (en) 2019-05-01 2025-05-27 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
WO2020242901A1 (en) * 2019-05-24 2020-12-03 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
US12391929B2 (en) 2019-05-24 2025-08-19 Element Biosciences, Inc. Polymerase-nucleotide conjugates for sequencing by trapping
JP2022537381A (ja) * 2019-06-20 2022-08-25 アリゾナ・ボード・オブ・リージェンツ・オン・ビハーフ・オブ・アリゾナ・ステイト・ユニバーシティー 核酸配列の直接電気的読み出し
JP2022537069A (ja) * 2019-06-21 2022-08-23 サーモ フィッシャー サイエンティフィック バルティックス ユーエービー 次世代配列決定ライブラリを調製するための核酸の標識化に有用なオリゴヌクレオチドが連結された三リン酸ヌクレオチド
TW202124406A (zh) 2019-09-10 2021-07-01 美商歐姆尼歐美公司 核苷酸之可逆修飾
US11287422B2 (en) 2019-09-23 2022-03-29 Element Biosciences, Inc. Multivalent binding composition for nucleic acid analysis
EP4045683B1 (en) 2019-10-18 2025-02-19 Pacific Biosciences of California, Inc. Methods for capping nucleic acids
WO2021092431A1 (en) 2019-11-08 2021-05-14 Omniome, Inc. Engineered polymerases for improved sequencing by binding
DE202019106695U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
DE202019106694U1 (de) 2019-12-02 2020-03-19 Omniome, Inc. System zur Sequenzierung von Nukleinsäuren in Fluidschaum
CA3173798A1 (en) 2020-03-03 2021-09-10 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
US20230183798A1 (en) 2020-05-05 2023-06-15 Pacific Biosciences Of California, Inc. Compositions and methods for modifying polymerase-nucleic acid complexes
WO2022006081A1 (en) * 2020-06-30 2022-01-06 Illumina, Inc. Catalytically controlled sequencing by synthesis to produce scarless dna
US20220356519A1 (en) 2021-05-10 2022-11-10 Pacific Biosciences Of California, Inc. Single-molecule seeding and amplification on a surface
WO2022240766A1 (en) 2021-05-10 2022-11-17 Pacific Biosciences Of California, Inc. Dna amplification buffer replenishment during rolling circle amplification
WO2023035108A1 (zh) * 2021-09-07 2023-03-16 深圳华大智造科技股份有限公司 一种用于分析靶多核苷酸的序列的方法
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
WO2025010160A1 (en) 2023-07-06 2025-01-09 Pacific Biosciences Of California, Inc. Methods and compositions for stabilizing concatemers
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005019476A1 (en) 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
WO2007091077A1 (en) 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
US7414116B2 (en) 2002-08-23 2008-08-19 Illumina Cambridge Limited Labelled nucleotides
US8071755B2 (en) 2004-05-25 2011-12-06 Helicos Biosciences Corporation Nucleotide analogs
US7264934B2 (en) 2004-06-10 2007-09-04 Ge Healthcare Bio-Sciences Corp. Rapid parallel nucleic acid analysis
DE602005020011D1 (de) * 2004-06-10 2010-04-29 Ge Healthcare Bio Sciences Verfahren zur nukleinsäureanalyse
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US7482120B2 (en) 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7544794B1 (en) 2005-03-11 2009-06-09 Steven Albert Benner Method for sequencing DNA and RNA by synthesis
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
US8962293B2 (en) * 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8026091B2 (en) * 2007-07-13 2011-09-27 Roche Molecular Systems, Inc. DNA polymerases and related methods
US8652781B2 (en) 2008-02-12 2014-02-18 Pacific Biosciences Of California, Inc. Cognate sampling kinetics
US8628940B2 (en) 2008-09-24 2014-01-14 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
WO2009145828A2 (en) 2008-03-31 2009-12-03 Pacific Biosciences Of California, Inc. Two slow-step polymerase enzyme systems and methods
AU2009251883B2 (en) 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
EP2607496B1 (en) 2008-12-23 2014-07-16 Illumina, Inc. Methods useful in nucleic acid sequencing protocols
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
LT2742151T (lt) 2011-08-10 2018-02-12 Life Technologies Corporation Polimerazės kompozicija
US9279154B2 (en) 2011-12-21 2016-03-08 Illumina, Inc. Apparatus and methods for kinetic analysis and determination of nucleic acid sequences
US9309501B2 (en) 2012-12-24 2016-04-12 Industrial Technology Research Institute Isolated DNA polymerases, kits and applications thereof
GB201301178D0 (en) 2013-01-23 2013-03-06 Dynamic Biosensors Gmbh Method for sequencing a template nucleic acid immobilized on a substrate
EP4219739A3 (en) 2013-09-30 2023-08-23 Life Technologies Corporation Polymerase compositions, methods of making and using same
US10077470B2 (en) 2015-07-21 2018-09-18 Omniome, Inc. Nucleic acid sequencing methods and systems
US10597643B2 (en) 2016-04-29 2020-03-24 Omniome, Inc. Polymerases engineered to reduce nucleotide-independent DNA binding
CN109154024B (zh) 2016-04-29 2022-03-04 欧姆尼欧美公司 核酸序列测定方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005019476A1 (en) 2003-08-20 2005-03-03 Applera Corporation Polymerase compositions
WO2007091077A1 (en) 2006-02-08 2007-08-16 Solexa Limited Method for sequencing a polynucleotide template
WO2010141390A2 (en) 2009-06-05 2010-12-09 Life Technologies Corporation Nucleotide transient binding for sequencing methods

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Mol Cell. 2006 Apr 7, 22(1):5-13
Nature. 2012 Jul 12, 487(7406): 196-201

Also Published As

Publication number Publication date
US10584379B2 (en) 2020-03-10
AU2017258523B2 (en) 2020-08-13
US20200199668A1 (en) 2020-06-25
CA3022431C (en) 2023-03-21
SG11201809522WA (en) 2018-11-29
EP3449011A1 (en) 2019-03-06
CA3022431A1 (en) 2017-11-02
AU2017258523A1 (en) 2018-11-22
US20170314072A1 (en) 2017-11-02
WO2017190012A1 (en) 2017-11-02
EP3449011B1 (en) 2020-11-04
JP6915939B2 (ja) 2021-08-11
KR20190003660A (ko) 2019-01-09
JP2019526225A (ja) 2019-09-19
US11976323B2 (en) 2024-05-07
CN109154024B (zh) 2022-03-04
CN109154024A (zh) 2019-01-04

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