JP2019520035A5 - - Google Patents
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- JP2019520035A5 JP2019520035A5 JP2018552853A JP2018552853A JP2019520035A5 JP 2019520035 A5 JP2019520035 A5 JP 2019520035A5 JP 2018552853 A JP2018552853 A JP 2018552853A JP 2018552853 A JP2018552853 A JP 2018552853A JP 2019520035 A5 JP2019520035 A5 JP 2019520035A5
- Authority
- JP
- Japan
- Prior art keywords
- pore
- monomer
- mutant
- variant
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000178 monomer Substances 0.000 claims description 72
- 102220498565 Serine/threonine-protein kinase N2_E94D_mutation Human genes 0.000 claims description 51
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- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 19
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- -1 (a) E84 Chemical compound 0.000 claims 1
- 102220483736 Amiloride-sensitive sodium channel subunit delta_H83K_mutation Human genes 0.000 claims 1
- 102220521062 Discoidin, CUB and LCCL domain-containing protein 1_K37Q_mutation Human genes 0.000 claims 1
- 102220475952 Hydroxycarboxylic acid receptor 1_V47N_mutation Human genes 0.000 claims 1
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- 235000001014 amino acid Nutrition 0.000 description 9
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- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
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- MKRBAPNEJMFMHU-UHFFFAOYSA-N 1-benzylpyrrole-2,5-dione Chemical compound O=C1C=CC(=O)N1CC1=CC=CC=C1 MKRBAPNEJMFMHU-UHFFFAOYSA-N 0.000 description 1
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 description 1
- 102220634571 AP-2 complex subunit alpha-1_E92V_mutation Human genes 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 102220502392 Putative C->U-editing enzyme APOBEC-4_E84A_mutation Human genes 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003606 oligomerizing effect Effects 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022041806A JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1605899.2 | 2016-04-06 | ||
| GB201605899 | 2016-04-06 | ||
| GB1608274.5 | 2016-05-11 | ||
| GBGB1608274.5A GB201608274D0 (en) | 2016-05-11 | 2016-05-11 | Mutant pore |
| PCT/GB2017/050961 WO2017174990A1 (en) | 2016-04-06 | 2017-04-06 | Mutant pore |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022041806A Division JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019520035A JP2019520035A (ja) | 2019-07-18 |
| JP2019520035A5 true JP2019520035A5 (https=) | 2020-05-14 |
| JP7364333B2 JP7364333B2 (ja) | 2023-10-18 |
Family
ID=58672620
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018552853A Active JP7364333B2 (ja) | 2016-04-06 | 2017-04-06 | 変異体ポア |
| JP2022041806A Active JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A Pending JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022041806A Active JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A Pending JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Country Status (8)
| Country | Link |
|---|---|
| US (4) | US11104709B2 (https=) |
| EP (3) | EP4122949B1 (https=) |
| JP (3) | JP7364333B2 (https=) |
| KR (1) | KR102472805B1 (https=) |
| CN (6) | CN118326019B (https=) |
| AU (1) | AU2017246690B2 (https=) |
| CA (2) | CA3020203A1 (https=) |
| WO (1) | WO2017174990A1 (https=) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| JP6271505B2 (ja) | 2012-04-10 | 2018-01-31 | オックスフォード ナノポール テクノロジーズ リミテッド | 変異体ライセニンポア |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| JP6749243B2 (ja) | 2014-01-22 | 2020-09-02 | オックスフォード ナノポール テクノロジーズ リミテッド | １つまたは複数のポリヌクレオチド結合タンパク質を標的ポリヌクレオチドに付着させる方法 |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| US10400014B2 (en) | 2014-09-01 | 2019-09-03 | Oxford Nanopore Technologies Ltd. | Mutant CsgG pores |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| AU2017225374B2 (en) | 2016-03-02 | 2020-03-05 | Oxford Nanopore Technologies Limited | Mutant pore |
| WO2017174990A1 (en) | 2016-04-06 | 2017-10-12 | Oxford Nanopore Technologies Limited | Mutant pore |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| WO2019002893A1 (en) | 2017-06-30 | 2019-01-03 | Vib Vzw | NEW PROTEIN PORES |
| GB202015993D0 (en) * | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN113896776B (zh) * | 2021-10-12 | 2024-02-06 | 成都齐碳科技有限公司 | 孔蛋白单体的突变体、蛋白孔及其应用 |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
| GB202413506D0 (en) | 2024-09-13 | 2024-10-30 | Oxford Nanopore Tech Plc | Pore monomers and their uses |
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-
2017
- 2017-04-06 WO PCT/GB2017/050961 patent/WO2017174990A1/en not_active Ceased
- 2017-04-06 CN CN202410563199.2A patent/CN118326019B/zh active Active
- 2017-04-06 CA CA3020203A patent/CA3020203A1/en active Pending
- 2017-04-06 CN CN202410563195.4A patent/CN118256605A/zh active Pending
- 2017-04-06 JP JP2018552853A patent/JP7364333B2/ja active Active
- 2017-04-06 CN CN202410563197.3A patent/CN118326018B/zh active Active
- 2017-04-06 EP EP22179411.8A patent/EP4122949B1/en active Active
- 2017-04-06 EP EP17722128.0A patent/EP3440098B1/en active Active
- 2017-04-06 EP EP24163939.2A patent/EP4397970A3/en active Pending
- 2017-04-06 CN CN202410563192.0A patent/CN118272512A/zh active Pending
- 2017-04-06 KR KR1020187029957A patent/KR102472805B1/ko active Active
- 2017-04-06 AU AU2017246690A patent/AU2017246690B2/en active Active
- 2017-04-06 CN CN201780022553.9A patent/CN109071618A/zh active Pending
- 2017-04-06 US US16/091,746 patent/US11104709B2/en active Active
- 2017-04-06 CN CN202310422087.0A patent/CN116514944A/zh active Pending
- 2017-04-06 CA CA3212147A patent/CA3212147A1/en active Pending
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- 2021-07-26 US US17/384,889 patent/US11939359B2/en active Active
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