JP2015519344A5 - - Google Patents
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- Publication number
- JP2015519344A5 JP2015519344A5 JP2015514137A JP2015514137A JP2015519344A5 JP 2015519344 A5 JP2015519344 A5 JP 2015519344A5 JP 2015514137 A JP2015514137 A JP 2015514137A JP 2015514137 A JP2015514137 A JP 2015514137A JP 2015519344 A5 JP2015519344 A5 JP 2015519344A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- reagent
- pore
- peptide
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 claims 27
- 235000018102 proteins Nutrition 0.000 claims 26
- 102000004169 proteins and genes Human genes 0.000 claims 26
- 108090000623 proteins and genes Proteins 0.000 claims 26
- 210000004027 cell Anatomy 0.000 claims 20
- 238000000034 method Methods 0.000 claims 16
- 108090000765 processed proteins & peptides Proteins 0.000 claims 16
- 239000011148 porous material Substances 0.000 claims 15
- 230000001086 cytosolic effect Effects 0.000 claims 8
- 230000004927 fusion Effects 0.000 claims 7
- 239000000758 substrate Substances 0.000 claims 7
- 210000004899 c-terminal region Anatomy 0.000 claims 6
- 230000003278 mimic effect Effects 0.000 claims 6
- 150000001875 compounds Chemical class 0.000 claims 5
- 102000035118 modified proteins Human genes 0.000 claims 5
- 108091005573 modified proteins Proteins 0.000 claims 5
- 108020001580 protein domains Proteins 0.000 claims 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 4
- 230000027455 binding Effects 0.000 claims 4
- 235000018417 cysteine Nutrition 0.000 claims 4
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 4
- 230000004001 molecular interaction Effects 0.000 claims 4
- 150000007970 thio esters Chemical class 0.000 claims 4
- 235000001014 amino acid Nutrition 0.000 claims 3
- 150000002366 halogen compounds Chemical class 0.000 claims 3
- 125000004573 morpholin-4-yl group Chemical group N1(CCOCC1)* 0.000 claims 3
- 150000007523 nucleic acids Chemical group 0.000 claims 3
- 230000001225 therapeutic effect Effects 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 230000005526 G1 to G0 transition Effects 0.000 claims 2
- 108090000279 Peptidyltransferases Proteins 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 229960002685 biotin Drugs 0.000 claims 2
- 235000020958 biotin Nutrition 0.000 claims 2
- 239000011616 biotin Substances 0.000 claims 2
- 150000005829 chemical entities Chemical class 0.000 claims 2
- 108020001507 fusion proteins Proteins 0.000 claims 2
- 102000037865 fusion proteins Human genes 0.000 claims 2
- 230000003993 interaction Effects 0.000 claims 2
- 230000009870 specific binding Effects 0.000 claims 2
- 108010001857 Cell Surface Receptors Proteins 0.000 claims 1
- 101150029707 ERBB2 gene Proteins 0.000 claims 1
- -1 amino acid chemical entity Chemical class 0.000 claims 1
- 230000001413 cellular effect Effects 0.000 claims 1
- 125000000151 cysteine group Chemical group N[C@@H](CS)C(=O)* 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 102000006240 membrane receptors Human genes 0.000 claims 1
- 108020004707 nucleic acids Proteins 0.000 claims 1
- 102000039446 nucleic acids Human genes 0.000 claims 1
- 230000012743 protein tagging Effects 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261649866P | 2012-05-21 | 2012-05-21 | |
| US201261649421P | 2012-05-21 | 2012-05-21 | |
| US61/649,421 | 2012-05-21 | ||
| US61/649,866 | 2012-05-21 | ||
| PCT/US2013/042118 WO2013177231A1 (en) | 2012-05-21 | 2013-05-21 | Translocation of non-natural chemical entities through anthrax protective antigen pore |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2015519344A JP2015519344A (ja) | 2015-07-09 |
| JP2015519344A5 true JP2015519344A5 (https=) | 2016-07-07 |
Family
ID=49624302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2015514137A Pending JP2015519344A (ja) | 2012-05-21 | 2013-05-21 | 炭疽菌防御抗原ポアを通しての非天然化学実体のトランスロケーション |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US9498538B2 (https=) |
| EP (1) | EP2852619A4 (https=) |
| JP (1) | JP2015519344A (https=) |
| CA (1) | CA2874436A1 (https=) |
| WO (2) | WO2013177231A1 (https=) |
Families Citing this family (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8673267B2 (en) | 2009-03-02 | 2014-03-18 | Massachusetts Institute Of Technology | Methods and products for in vivo enzyme profiling |
| JP6245990B2 (ja) | 2011-03-15 | 2017-12-13 | マサチューセッツ インスティテュート オブ テクノロジー | 同位体によりコード化されたレポーターによる多重検出 |
| CA2891510C (en) | 2012-11-16 | 2022-10-18 | Transposagen Biopharmaceuticals, Inc. | Site-specific enzymes and methods of use |
| EP2777714A1 (en) | 2013-03-15 | 2014-09-17 | NBE-Therapeutics LLC | Method of producing an immunoligand/payload conjugate by means of a sequence-specific transpeptidase enzyme |
| JP6847660B2 (ja) | 2013-06-07 | 2021-03-24 | マサチューセッツ インスティテュート オブ テクノロジー | リガンドをコードする合成バイオマーカーのアフィニティベースの検出 |
| US20170107541A1 (en) * | 2014-06-17 | 2017-04-20 | Poseida Therapeutics, Inc. | A method for directing proteins to specific loci in the genome and uses thereof |
| KR20170098821A (ko) | 2014-12-17 | 2017-08-30 | 에프. 호프만-라 로슈 아게 | 소르타아제를 사용한 １ 단계로의 이중 폴리펩티드 컨쥬게이션을 위한 효소 원-포트 반응 |
| EP3234177A1 (en) | 2014-12-17 | 2017-10-25 | F. Hoffmann-La Roche AG | Activity assay for bond forming enzymes |
| US11473082B2 (en) | 2015-06-17 | 2022-10-18 | Poseida Therapeutics, Inc. | Compositions and methods for directing proteins to specific loci in the genome |
| BR112018003782A2 (pt) | 2015-08-27 | 2018-09-25 | Ipsen Pharma Sas | composições e métodos para o tratamento da dor |
| WO2017050866A1 (en) | 2015-09-25 | 2017-03-30 | F. Hoffmann-La Roche Ag | Novel soluble sortase a |
| CN108026560A (zh) | 2015-09-25 | 2018-05-11 | 豪夫迈·罗氏有限公司 | 在低共熔溶剂中使用分选酶的转酰胺反应 |
| CN108138204B (zh) * | 2015-09-25 | 2021-12-31 | 豪夫迈·罗氏有限公司 | 使用分选酶a生产硫酯的方法 |
| EP3440013A4 (en) | 2016-04-08 | 2021-03-17 | Massachusetts Institute of Technology | METHOD FOR SPECIFIC PROFILING OF PROTEASE ACTIVITY ON LYMPH NODES |
| WO2017193070A1 (en) | 2016-05-05 | 2017-11-09 | Massachusetts Institute Of Technology | Methods and uses for remotely triggered protease activity measurements |
| US10738338B2 (en) | 2016-10-18 | 2020-08-11 | The Research Foundation for the State University | Method and composition for biocatalytic protein-oligonucleotide conjugation and protein-oligonucleotide conjugate |
| CA3059358A1 (en) | 2017-04-07 | 2018-10-11 | Massachusetts Institute Of Technology | Methods to spatially profile protease activity in tissue and sections |
| CN108504670B (zh) * | 2018-02-27 | 2022-01-04 | 温州医科大学 | 一种大肠杆菌冷休克助溶型表达质粒的构建方法及其应用 |
| WO2019173332A1 (en) | 2018-03-05 | 2019-09-12 | Massachusetts Institute Of Technology | Inhalable nanosensors with volatile reporters and uses thereof |
| US11739128B2 (en) | 2018-03-07 | 2023-08-29 | Epicypher, Inc. | Peptide ligase-mediated engineering of recombinant nucleosomes |
| AU2019273119B2 (en) * | 2018-05-22 | 2022-11-24 | The University Of Sydney | New synthetic methods using native chemical ligation in flow |
| US12173349B2 (en) | 2018-09-25 | 2024-12-24 | Massachusetts Institute Of Technology | Lung protease nanosensors and uses thereof |
| US11835522B2 (en) | 2019-01-17 | 2023-12-05 | Massachusetts Institute Of Technology | Sensors for detecting and imaging of cancer metastasis |
| WO2023178327A1 (en) * | 2022-03-17 | 2023-09-21 | Ohio State Innovation Foundation | Membrane translocation domains and uses thereof |
| EP4519308A4 (en) * | 2022-05-04 | 2026-03-11 | Ohio State Innovation Foundation | COMPOSITIONS FOR THE DISTRIBUTION OF AGENTS IN PLANT CELLS |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5677274A (en) * | 1993-02-12 | 1997-10-14 | The Government Of The United States As Represented By The Secretary Of The Department Of Health And Human Services | Anthrax toxin fusion proteins and related methods |
| US5723125A (en) | 1995-12-28 | 1998-03-03 | Tanox Biosystems, Inc. | Hybrid with interferon-alpha and an immunoglobulin Fc linked through a non-immunogenic peptide |
| US5770380A (en) | 1996-09-13 | 1998-06-23 | University Of Pittsburgh | Synthetic antibody mimics--multiple peptide loops attached to a molecular scaffold |
| AU745094B2 (en) | 1997-06-13 | 2002-03-14 | Gryphon Sciences | Solid phase native chemical ligation of unprotected or N-terminal cysteine protected peptides in aqueous solution |
| GB0030038D0 (en) * | 2000-12-08 | 2001-01-24 | Univ Warwick | Yeast-based assay |
| WO2003074554A2 (en) * | 2002-03-05 | 2003-09-12 | Fundacion Galileo | Apoptogenic-bacteriocins combining broad spectrum antibiotic and selective anti-tumoral activities, and compositions and uses thereof |
| WO2005051976A2 (en) | 2003-11-20 | 2005-06-09 | Ansata Therapeutics, Inc. | Protein and peptide ligation processes and one-step purification processes |
| WO2007100358A2 (en) | 2005-10-07 | 2007-09-07 | University Of Chicago | Convergent synthesis of proteins by kinetically controlled ligation |
| WO2007083175A1 (en) * | 2006-01-17 | 2007-07-26 | West China Hospital, Sichuan University | Antiviral bifunctional molecules, methods of construction and methods of treating virus-induced cancer therewith |
| WO2011133704A2 (en) * | 2010-04-20 | 2011-10-27 | Whitehead Institute For Biomedical Researh | Modified polypeptides and proteins and uses thereof |
| WO2012096926A2 (en) | 2011-01-10 | 2012-07-19 | President And Fellows Of Harvard College | Method for delivering agents into cells using bacterial toxins |
| WO2012142659A1 (en) * | 2011-04-19 | 2012-10-26 | Baker Idi Heart And Diabetes Institute Holdings Limited | Site-selective modification of proteins |
| CA2865243A1 (en) * | 2012-02-23 | 2013-08-29 | President And Fellows Of Harvard College | Modified microbial toxin receptor for delivering agents into cells |
-
2013
- 2013-05-21 WO PCT/US2013/042118 patent/WO2013177231A1/en not_active Ceased
- 2013-05-21 JP JP2015514137A patent/JP2015519344A/ja active Pending
- 2013-05-21 WO PCT/US2013/042107 patent/WO2013177221A1/en not_active Ceased
- 2013-05-21 US US14/402,856 patent/US9498538B2/en active Active
- 2013-05-21 US US14/402,915 patent/US9731029B2/en active Active
- 2013-05-21 EP EP13793070.7A patent/EP2852619A4/en not_active Withdrawn
- 2013-05-21 CA CA2874436A patent/CA2874436A1/en not_active Abandoned
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