JP2019520035A5 - - Google Patents
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- JP2019520035A5 JP2019520035A5 JP2018552853A JP2018552853A JP2019520035A5 JP 2019520035 A5 JP2019520035 A5 JP 2019520035A5 JP 2018552853 A JP2018552853 A JP 2018552853A JP 2018552853 A JP2018552853 A JP 2018552853A JP 2019520035 A5 JP2019520035 A5 JP 2019520035A5
- Authority
- JP
- Japan
- Prior art keywords
- pore
- monomer
- mutant
- variant
- polynucleotide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000178 monomer Substances 0.000 claims description 72
- 102220498565 Serine/threonine-protein kinase N2_E94D_mutation Human genes 0.000 claims description 51
- 239000011148 porous material Substances 0.000 claims description 44
- 102000040430 polynucleotide Human genes 0.000 claims description 42
- 108091033319 polynucleotide Proteins 0.000 claims description 42
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- 150000001413 amino acids Chemical class 0.000 claims description 20
- 238000006467 substitution reaction Methods 0.000 claims description 20
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 19
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- 102220532368 Serpin B10_K99Q_mutation Human genes 0.000 claims description 4
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- 102220588493 Hemoglobin subunit beta_T51K_mutation Human genes 0.000 claims description 3
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- -1 (a) E84 Chemical compound 0.000 claims 1
- 102220483736 Amiloride-sensitive sodium channel subunit delta_H83K_mutation Human genes 0.000 claims 1
- 102220521062 Discoidin, CUB and LCCL domain-containing protein 1_K37Q_mutation Human genes 0.000 claims 1
- 102220475952 Hydroxycarboxylic acid receptor 1_V47N_mutation Human genes 0.000 claims 1
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- 235000001014 amino acid Nutrition 0.000 description 9
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- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 2
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 2
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- 102200091898 rs77050410 Human genes 0.000 description 2
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- MKRBAPNEJMFMHU-UHFFFAOYSA-N 1-benzylpyrrole-2,5-dione Chemical compound O=C1C=CC(=O)N1CC1=CC=CC=C1 MKRBAPNEJMFMHU-UHFFFAOYSA-N 0.000 description 1
- 125000000094 2-phenylethyl group Chemical group [H]C1=C([H])C([H])=C(C([H])=C1[H])C([H])([H])C([H])([H])* 0.000 description 1
- 102220634571 AP-2 complex subunit alpha-1_E92V_mutation Human genes 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- 102220502392 Putative C->U-editing enzyme APOBEC-4_E84A_mutation Human genes 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 125000001909 leucine group Chemical group [H]N(*)C(C(*)=O)C([H])([H])C(C([H])([H])[H])C([H])([H])[H] 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000003606 oligomerizing effect Effects 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022041806A JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1605899.2 | 2016-04-06 | ||
| GB201605899 | 2016-04-06 | ||
| GB1608274.5 | 2016-05-11 | ||
| GBGB1608274.5A GB201608274D0 (en) | 2016-05-11 | 2016-05-11 | Mutant pore |
| PCT/GB2017/050961 WO2017174990A1 (en) | 2016-04-06 | 2017-04-06 | Mutant pore |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022041806A Division JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019520035A JP2019520035A (ja) | 2019-07-18 |
| JP2019520035A5 true JP2019520035A5 (cg-RX-API-DMAC7.html) | 2020-05-14 |
| JP7364333B2 JP7364333B2 (ja) | 2023-10-18 |
Family
ID=58672620
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018552853A Active JP7364333B2 (ja) | 2016-04-06 | 2017-04-06 | 変異体ポア |
| JP2022041806A Active JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A Pending JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022041806A Active JP7555987B2 (ja) | 2016-04-06 | 2022-03-16 | 変異体ポア |
| JP2024095022A Pending JP2024138262A (ja) | 2016-04-06 | 2024-06-12 | 変異体ポア |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US11104709B2 (cg-RX-API-DMAC7.html) |
| EP (3) | EP4397970A3 (cg-RX-API-DMAC7.html) |
| JP (3) | JP7364333B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102472805B1 (cg-RX-API-DMAC7.html) |
| CN (5) | CN118272512A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2017246690B2 (cg-RX-API-DMAC7.html) |
| CA (2) | CA3212147A1 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2017174990A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0905140D0 (en) | 2009-03-25 | 2009-05-06 | Isis Innovation | Method |
| KR102083695B1 (ko) | 2012-04-10 | 2020-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 리세닌 기공 |
| GB201313477D0 (en) | 2013-07-29 | 2013-09-11 | Univ Leuven Kath | Nanopore biosensors for detection of proteins and nucleic acids |
| EP3097210B2 (en) | 2014-01-22 | 2022-04-20 | Oxford Nanopore Technologies Limited | Method for attaching one or more polynucleotide binding proteins to a target polynucleotide |
| US10443097B2 (en) | 2014-05-02 | 2019-10-15 | Oxford Nanopore Technologies Ltd. | Method of improving the movement of a target polynucleotide with respect to a transmembrane pore |
| CN117164683A (zh) | 2014-09-01 | 2023-12-05 | 弗拉芒区生物技术研究所 | 突变csgg孔 |
| GB201502810D0 (en) | 2015-02-19 | 2015-04-08 | Oxford Nanopore Tech Ltd | Method |
| US11169138B2 (en) | 2015-04-14 | 2021-11-09 | Katholieke Universiteit Leuven | Nanopores with internal protein adaptors |
| KR102222192B1 (ko) | 2016-03-02 | 2021-03-02 | 옥스포드 나노포어 테크놀로지즈 리미티드 | 돌연변이체 기공 |
| EP4397970A3 (en) | 2016-04-06 | 2024-10-09 | Oxford Nanopore Technologies PLC | Mutant pore |
| GB201707122D0 (en) | 2017-05-04 | 2017-06-21 | Oxford Nanopore Tech Ltd | Pore |
| CN119192308A (zh) | 2017-06-30 | 2024-12-27 | 弗拉芒区生物技术研究所 | 新颖蛋白孔 |
| GB202015993D0 (en) * | 2020-10-08 | 2020-11-25 | Oxford Nanopore Tech Ltd | Method |
| CN113896776B (zh) * | 2021-10-12 | 2024-02-06 | 成都齐碳科技有限公司 | 孔蛋白单体的突变体、蛋白孔及其应用 |
| GB202216905D0 (en) | 2022-11-11 | 2022-12-28 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
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| AU8586298A (en) | 1997-07-25 | 1999-02-16 | University Of Massachusetts | Designed protein pores as components for biosensors |
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2017
- 2017-04-06 EP EP24163939.2A patent/EP4397970A3/en active Pending
- 2017-04-06 CN CN202410563192.0A patent/CN118272512A/zh active Pending
- 2017-04-06 KR KR1020187029957A patent/KR102472805B1/ko active Active
- 2017-04-06 CN CN201780022553.9A patent/CN109071618A/zh active Pending
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- 2017-04-06 CA CA3212147A patent/CA3212147A1/en active Pending
- 2017-04-06 CN CN202410563197.3A patent/CN118326018B/zh active Active
- 2017-04-06 WO PCT/GB2017/050961 patent/WO2017174990A1/en not_active Ceased
- 2017-04-06 EP EP17722128.0A patent/EP3440098B1/en active Active
- 2017-04-06 CN CN202310422087.0A patent/CN116514944A/zh active Pending
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