JP2019519242A - 細菌ヘモグロビンライブラリーを生成するための方法およびその使用 - Google Patents

細菌ヘモグロビンライブラリーを生成するための方法およびその使用 Download PDF

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JP2019519242A
JP2019519242A JP2018568373A JP2018568373A JP2019519242A JP 2019519242 A JP2019519242 A JP 2019519242A JP 2018568373 A JP2018568373 A JP 2018568373A JP 2018568373 A JP2018568373 A JP 2018568373A JP 2019519242 A JP2019519242 A JP 2019519242A
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seq
bacterial
library
gene
genes
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JP2019519242A5 (enExample
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ショーン マンチェスター,
ショーン マンチェスター,
アレクサンダー ネッケルマン,
アレクサンダー ネッケルマン,
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Zymergen Inc
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JP2018568373A 2016-06-30 2017-06-28 細菌ヘモグロビンライブラリーを生成するための方法およびその使用 Pending JP2019519242A (ja)

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US201662356934P 2016-06-30 2016-06-30
US62/356,934 2016-06-30
PCT/US2017/039772 WO2018005655A2 (en) 2016-06-30 2017-06-28 Methods for generating a bacterial hemoglobin library and uses thereof

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JP2019519242A true JP2019519242A (ja) 2019-07-11
JP2019519242A5 JP2019519242A5 (enExample) 2020-08-06

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US (2) US10544390B2 (enExample)
EP (1) EP3478833A4 (enExample)
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KR (1) KR102345899B1 (enExample)
WO (1) WO2018005655A2 (enExample)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
US10544390B2 (en) 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. METHOD OF GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF
KR20200026881A (ko) * 2017-06-07 2020-03-11 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터 및 보조 유전자 발현을 조절하는 데 이의 용도
SG11202104409YA (en) * 2018-10-31 2021-05-28 Zymergen Inc Multiplexed deterministic assembly of dna libraries
CN112300971B (zh) * 2019-07-26 2022-12-23 安徽吐露港生物科技有限公司 高通量基因合成法提高食气梭菌一氧化碳利用率的方法
JP2023553042A (ja) * 2020-12-02 2023-12-20 プランイート フーズ プライベート リミテッド 組換えによりグロビンポリペプチドを生成する方法および肉代替食品製品
JP7548886B2 (ja) 2021-09-28 2024-09-10 信越化学工業株式会社 有機膜形成用組成物、パターン形成方法並びに有機膜形成用化合物及び重合体

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992003546A1 (en) * 1990-08-23 1992-03-05 Exogene Corporation Enhancement of production of native products in corynebacterium by expression of cloned bacterial hemoglobin
WO2006028063A1 (ja) * 2004-09-09 2006-03-16 Research Institute Of Innovative Technology For The Earth プロモーター機能を有するdna断片
US20060269975A1 (en) * 1999-07-16 2006-11-30 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis

Family Cites Families (195)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5835197A (ja) 1981-08-26 1983-03-01 Kyowa Hakko Kogyo Co Ltd プラスミドpcg2
US4435504A (en) 1982-07-15 1984-03-06 Syva Company Immunochromatographic assay with support having bound "MIP" and second enzyme
US4601983A (en) 1983-06-15 1986-07-22 Ajinomoto Co., Inc. Coryneform bacteria carrying recombinant plasmids and their use in the fermentative production of L-threonine and L-isoleucine
US4601893A (en) 1984-02-08 1986-07-22 Pfizer Inc. Laminate device for controlled and prolonged release of substances to an ambient environment and method of use
GB8406752D0 (en) 1984-03-15 1984-04-18 Unilever Plc Chemical and clinical tests
GB2165546B (en) 1984-08-21 1989-05-17 Asahi Chemical Ind A plasmid containing a gene for tetracycline resistance and dna fragments derived therefrom
JPH0655149B2 (ja) 1985-03-12 1994-07-27 協和醗酵工業株式会社 L―リジンの製造法
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
CA1303983C (en) 1987-03-27 1992-06-23 Robert W. Rosenstein Solid phase assay
US4855240A (en) 1987-05-13 1989-08-08 Becton Dickinson And Company Solid phase assay employing capillary flow
IL88095A0 (en) 1987-10-23 1989-06-30 California Inst Of Techn Dna sequences,proteins and gene preparations
JPH01225487A (ja) 1988-03-04 1989-09-08 Sakai Eng Kk セルロースのアスペルギルス ニガーによるクエン酸若しくはグルコン酸生産を目的としたバイオリアクター担体への利用方法
JP2748418B2 (ja) 1988-08-03 1998-05-06 味の素株式会社 組換えdna、該組換えdnaを有する微生物
US5976843A (en) 1992-04-22 1999-11-02 Ajinomoto Co., Inc. Bacterial strain of Escherichia coli BKIIM B-3996 as the producer of L-threonine
CA2072115A1 (en) 1989-10-30 1991-05-01 Dallas E. Hughes Expression of bacterial hemoglobin and enhancement of expression of cloned and native products in streptomyces
DE4027453A1 (de) 1990-08-30 1992-03-05 Degussa Neue plasmide aus corynebacterium glutamicum und davon abgeleitete plasmidvektoren
JP3023615B2 (ja) 1990-08-30 2000-03-21 協和醗酵工業株式会社 発酵法によるl―トリプトファンの製造法
DE69133559T2 (de) 1990-09-27 2007-11-22 Invitrogen Corp., Carlsbad Direkte Klonierung von PCR amplifizierten Nucleinsäuren
US6060296A (en) 1991-07-03 2000-05-09 The Salk Institute For Biological Studies Protein kinases
DE4130867A1 (de) 1991-09-17 1993-03-18 Degussa Verfahren zur fermentativen herstellung von aminosaeuren
US5516670A (en) 1991-09-30 1996-05-14 Kuehnle; Adelheid R. Magnetophoretic particle delivery method and apparatus for the treatment of cells
DE69432543T2 (de) 1993-07-23 2003-12-24 Dsm N.V., Te Heerlen Selektionmarker-genfreie rekombinante Stämme: Verfahren zur ihrer Herstellung und die Verwendung dieser Stämme
US6335160B1 (en) 1995-02-17 2002-01-01 Maxygen, Inc. Methods and compositions for polypeptide engineering
US5837458A (en) 1994-02-17 1998-11-17 Maxygen, Inc. Methods and compositions for cellular and metabolic engineering
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US5928905A (en) 1995-04-18 1999-07-27 Glaxo Group Limited End-complementary polymerase reaction
US6117679A (en) 1994-02-17 2000-09-12 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US5834252A (en) 1995-04-18 1998-11-10 Glaxo Group Limited End-complementary polymerase reaction
US6090592A (en) 1994-08-03 2000-07-18 Mosaic Technologies, Inc. Method for performing amplification of nucleic acid on supports
DE4440118C1 (de) 1994-11-11 1995-11-09 Forschungszentrum Juelich Gmbh Die Genexpression in coryneformen Bakterien regulierende DNA
KR100420743B1 (ko) 1994-12-09 2004-05-24 아지노모토 가부시키가이샤 신규한리신데카복실라제유전자및l-리신의제조방법
AU706285B2 (en) 1995-05-16 1999-06-10 Ajinomoto Co., Inc. Feed additive
GB2304718B (en) 1995-09-05 2000-01-19 Degussa The production of tryptophan by the bacterium escherichia coli
US6713279B1 (en) 1995-12-07 2004-03-30 Diversa Corporation Non-stochastic generation of genetic vaccines and enzymes
US20030215798A1 (en) 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
US6479258B1 (en) 1995-12-07 2002-11-12 Diversa Corporation Non-stochastic generation of genetic vaccines
US6537776B1 (en) 1999-06-14 2003-03-25 Diversa Corporation Synthetic ligation reassembly in directed evolution
US6358709B1 (en) 1995-12-07 2002-03-19 Diversa Corporation End selection in directed evolution
DE19548222A1 (de) 1995-12-22 1997-06-26 Forschungszentrum Juelich Gmbh Verfahren zur mikrobiellen Herstellung von Aminosäuren durch gesteigerte Aktivität von Exportcarriern
US5753477A (en) 1996-03-19 1998-05-19 University Technology Corporation Magneto-biolistic methods
EP0954586A1 (en) 1996-10-18 1999-11-10 Baxter Biotech Technology S.A.R.L. Methods of reducing the levels of protoporphyrin ix in recombinant hemoglobin preparations
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
US7148054B2 (en) 1997-01-17 2006-12-12 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
KR100570935B1 (ko) 1997-01-17 2006-04-13 맥시겐, 인크. 반복적 서열 재조합에 의한 전세포 및 유기체의 개량 방법
JP2002503954A (ja) 1997-04-01 2002-02-05 グラクソ、グループ、リミテッド 核酸増幅法
JPH1180185A (ja) 1997-09-05 1999-03-26 Res Dev Corp Of Japan オリゴヌクレオチドの化学合成法
DE19831609B4 (de) 1997-10-04 2009-11-12 Evonik Degussa Gmbh Verfahren zur Herstellung von Aminosäuren der Aspartat- und/oder Glutamatfamilie und im Verfahren einsetzbare Mittel
US5990350A (en) 1997-12-16 1999-11-23 Archer Midland Company Process for making granular L-lysine
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
KR100618495B1 (ko) 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6759195B1 (en) 1999-03-25 2004-07-06 University Of Maryland Biotechnology Institute Method of differential display of prokaryotic messenger RNA by RTPCR
US6300070B1 (en) 1999-06-04 2001-10-09 Mosaic Technologies, Inc. Solid phase methods for amplifying multiple nucleic acids
US20030049804A1 (en) 1999-06-25 2003-03-13 Markus Pompejus Corynebacterium glutamicum genes encoding metabolic pathway proteins
US6962989B1 (en) 1999-07-08 2005-11-08 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding novel proteins
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
MXPA01013201A (es) 1999-08-12 2002-06-04 Maxygen Inc Redistribucion de adn de genes de dioxigenasa para la produccion de quimicos industriales.
US7413536B1 (en) 1999-09-14 2008-08-19 Xenoport, Inc. Substrates and screening methods for transport proteins
US6586214B1 (en) 1999-09-15 2003-07-01 Degussa Ag Method for increasing the metabolic flux through the pentose phosphate cycle in coryneform bacteria by regulation of the phosphoglucose isomerase (pgi gene)
US7033781B1 (en) 1999-09-29 2006-04-25 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
US6713289B2 (en) 1999-10-05 2004-03-30 Degussa Ag Nucleotide sequences which code for the eno gene
DE19947791A1 (de) 1999-10-05 2001-04-12 Degussa Neue für das eno-Gen codierende Nukleotidsequenzen
DE19950409A1 (de) 1999-10-20 2001-04-26 Degussa Neue für das pck-Gen codierende Nukleotidsequenzen
DE19959327A1 (de) 1999-12-09 2001-06-13 Degussa Neue für das zwa2-Gen codierende Nukleotidsequenzen
DE19959328A1 (de) 1999-12-09 2001-06-13 Degussa Neue für das zwa1-Gen codierende Nukleotidsequenzen
JP4623825B2 (ja) 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
US20020081673A1 (en) 2000-06-02 2002-06-27 Bettina Mockel Novel nucleotide sequences coding for the glbO gene
SE0002460D0 (sv) 2000-06-29 2000-06-29 Roslyn Bill Modified yeast
WO2002029032A2 (en) 2000-09-30 2002-04-11 Diversa Corporation Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
IL155154A0 (en) 2000-09-30 2003-10-31 Diversa Corp Whole cell engineering by mutagenizing a substantial portion of a starting genome, combining mutations, and optionally repeating
CA2881568C (en) 2000-10-27 2019-09-24 Novartis Vaccines And Diagnostics, Inc. Nucleic acids and proteins from streptococcus groups a & b
JP4776146B2 (ja) 2001-01-10 2011-09-21 ザ・ペン・ステート・リサーチ・ファンデーション 細胞代謝をモデル化する方法及びシステム
US20020169562A1 (en) 2001-01-29 2002-11-14 Gregory Stephanopoulos Defining biological states and related genes, proteins and patterns
US7127379B2 (en) 2001-01-31 2006-10-24 The Regents Of The University Of California Method for the evolutionary design of biochemical reaction networks
US20030027175A1 (en) 2001-02-13 2003-02-06 Gregory Stephanopoulos Dynamic whole genome screening methodology and systems
GB0110432D0 (en) 2001-04-27 2001-06-20 Plant Bioscience Ltd Lantibiotic production
CA2456416A1 (en) 2001-08-06 2003-02-20 Degussa Ag Coryneform bacteria which produce chemical compounds ii
CA2455878A1 (en) 2001-08-06 2003-05-15 Degussa Ag Production of l-lysine by genetically modified corynebacterium glutamicum strains
US7160711B2 (en) 2001-08-06 2007-01-09 Degussa Ag Coryneform bacteria which produce chemical compounds I
US7826975B2 (en) 2002-07-10 2010-11-02 The Penn State Research Foundation Method for redesign of microbial production systems
US7563600B2 (en) 2002-09-12 2009-07-21 Combimatrix Corporation Microarray synthesis and assembly of gene-length polynucleotides
KR101130587B1 (ko) 2002-10-04 2012-03-30 다니스코 유에스 인크. 생물질 제조용 글루코오스 수송 돌연변이
US20050079617A1 (en) 2003-10-03 2005-04-14 Cervin Marguerite A. Glucose transport mutants for production of biomaterial
US20040115304A1 (en) 2002-12-16 2004-06-17 Frank Dubner Feesdstuffs additives containing L-lysine with improved abrasion resistance, and process for their production
DE10331366A1 (de) 2003-07-11 2005-01-27 Degussa Ag Verfahren zur Granulation eines Tierfuttermittel-Zusatzes
WO2005021772A1 (en) 2003-08-29 2005-03-10 Degussa Ag Process for the preparation of l-lysine
DK1781785T3 (da) 2004-07-20 2011-02-14 Novozymes Inc Fremgangsmåde til fremstilling af mutant-polynukleotider
US20070166792A1 (en) * 2004-09-15 2007-07-19 Olson John S Increasing hemoglobin and other heme protein production in bacteria by co-expression of heme transport genes
US7987056B2 (en) 2004-09-20 2011-07-26 The Regents Of The University Of Colorado, A Body Corporate Mixed-library parallel gene mapping quantitative micro-array technique for genome-wide identification of trait conferring genes
DE102004061846A1 (de) 2004-12-22 2006-07-13 Basf Ag Mehrfachpromotoren
WO2006092449A2 (en) 2005-03-02 2006-09-08 Metanomics Gmbh Process for the production of fine chemicals
WO2006108075A2 (en) 2005-04-05 2006-10-12 The Scripps Research Institute Compositions and methods for enhancing drug sensitivity and treating drug resistant infections an diseases
MX2007013870A (es) 2005-05-05 2008-01-24 Monsanto Technology Llc Plantas que contienen un gen heterologo de flavohemoglobina y sus metodos de uso.
KR20070009269A (ko) 2005-07-15 2007-01-18 한국생명공학연구원 재조합단백질 생산용 단백질융합인자 라이브러리 및이로부터 획득된 단백질융합인자
WO2007012078A1 (en) 2005-07-18 2007-01-25 Basf Ag Methionine producing recombinant microorganisms
US7846688B2 (en) 2005-08-15 2010-12-07 The Regents Of The University Of Colorado Broad host range vectors for shotgun and expression library cloning in Gram negative bacteria
DE102005048818A1 (de) 2005-10-10 2007-04-12 Degussa Ag Mikrobiologische Herstellung von 3-Hydroxypropionsäure
KR100789271B1 (ko) 2005-11-30 2008-01-02 씨제이 주식회사 L-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 l-라이신을 생산하는 방법
US7498413B2 (en) 2006-01-06 2009-03-03 Pioneer Hi-Bred International, Inc. Maize antimicrobial protein useful for enhancing plant resistance to pathogens
WO2007130745A1 (en) 2006-03-15 2007-11-15 Regents Of The University Of Colorado Enhanced alcohol tolerant microorganism and methods of use thereof
US8445639B2 (en) 2006-05-15 2013-05-21 Avidbiotics Corporation Recombinant bacteriophage and methods for their use
EP2021489A2 (en) 2006-05-30 2009-02-11 Dow Global Technologies Inc. Codon optimization method
GB0611444D0 (en) 2006-06-09 2006-07-19 Medical Res Council Technology Rnase H2 complex and genes therefor
US20100048938A1 (en) 2006-06-22 2010-02-25 Marco Alexander Van Den Berg Fermentation of pravastatin
EP2032703B1 (en) 2006-06-26 2011-03-23 DSM IP Assets B.V. High throughput transfection of filamentous fungi
US20080103060A1 (en) 2006-08-08 2008-05-01 Regents Of The University Of Colorado Compositions and methods for chemical reporter vectors
US9862956B2 (en) * 2006-12-10 2018-01-09 Danisco Us Inc. Expression and high-throughput screening of complex expressed DNA libraries in filamentous fungi
CA2675026A1 (en) 2007-01-12 2008-07-24 The Regents Of The University Of Colorado, A Body Corporate Compositions and methods for enhancing tolerance for the production of organic chemicals produced by microorganisms
US20080243397A1 (en) 2007-03-30 2008-10-02 Jean Peccoud Software for design and verification of synthetic genetic constructs
US9580719B2 (en) 2007-04-27 2017-02-28 Pfenex, Inc. Method for rapidly screening microbial hosts to identify certain strains with improved yield and/or quality in the expression of heterologous proteins
KR100898246B1 (ko) * 2007-08-17 2009-05-18 씨제이제일제당 (주) 신규한 코리네박테리움 글루타미쿰 프로모터
AU2008300579B2 (en) 2007-09-18 2014-11-13 Basf Plant Science Gmbh Plants with increased yield
WO2009043372A1 (en) 2007-10-02 2009-04-09 Metabolic Explorer Increasing methionine yield
MX2010011068A (es) 2008-04-08 2010-11-04 Amyris Biotechnologies Inc Expresion de secuencias heterologas.
CN102317436B (zh) 2008-07-23 2014-06-25 Opx生物工艺学公司 用于增加微生物对3-羟基丙酸(3-hp)的耐受性以及增加3-羟基丙酸产量的方法、系统和组合物
WO2010025310A2 (en) 2008-08-27 2010-03-04 Westend Asset Clearinghouse Company, Llc Methods and devices for high fidelity polynucleotide synthesis
US8530203B2 (en) 2008-09-01 2013-09-10 Shinshu University Process for producing useful substance
CN105671027A (zh) 2008-09-30 2016-06-15 诺维信股份有限公司 在丝状真菌细胞中使用阳性和阴性选择性基因的方法
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
AU2009316660B2 (en) 2008-11-19 2015-01-29 Amrys, Inc. Compositions and methods for the assembly of polynucleotides
AU2009319722B2 (en) 2008-11-28 2016-08-04 Corbion Biotech, Inc. Production of tailored oils in heterotrophic microorganisms
WO2010075424A2 (en) 2008-12-22 2010-07-01 The Regents Of University Of California Compositions and methods for downregulating prokaryotic genes
EP2398915B1 (en) 2009-02-20 2016-08-24 Synthetic Genomics, Inc. Synthesis of sequence-verified nucleic acids
US20130217132A1 (en) 2009-05-01 2013-08-22 Ryan T. Gill Methods, compositions and use for enhancing chemical tolerance by microorganisms
US20100317115A1 (en) 2009-05-01 2010-12-16 Ryan Gill Methods, compositions and uses for enhancing chemical tolerance by microorganisms
US8574835B2 (en) 2009-05-29 2013-11-05 Life Technologies Corporation Scaffolded nucleic acid polymer particles and methods of making and using
WO2010141468A1 (en) 2009-06-01 2010-12-09 Way Jeffrey C Methods and molecules for yield improvement involving metabolic engineering
US10541046B2 (en) 2009-09-02 2020-01-21 Microsoft Technology Licensing, Llc Creating genetic devices
MX2012003604A (es) 2009-09-27 2012-09-12 Opx Biotechnologies Inc Metodo para producir acido 3-hidroxipropionico y otros productos.
EP2529023A4 (en) 2010-01-27 2015-06-10 Opx Biotechnologies Inc MICRO-ORGANISM MANUFACTURE FROM HIGH-FLOW CHEMICAL PRODUCTS AND CORRESPONDING COMPOSITIONS, METHODS AND SYSTEMS
US10087431B2 (en) 2010-03-10 2018-10-02 The Regents Of The University Of California Methods of generating nucleic acid fragments
CA2801799C (en) 2010-06-03 2018-11-20 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
EP2395087A1 (en) 2010-06-11 2011-12-14 Icon Genetics GmbH System and method of modular cloning
BR112013014688B1 (pt) 2010-12-13 2021-09-28 Myriant Corporation Bactérias escherichia coli geneticamente alteradas
US20150368639A1 (en) 2011-04-14 2015-12-24 Ryan T. Gill Compositions, methods and uses for multiplex protein sequence activity relationship mapping
US20120264902A1 (en) 2011-04-18 2012-10-18 The Regents Of The University Of Colorado, A Body Corporate Methods, Systems and Compositions for Increased Microorganism Tolerance to and Production of 3-Hydroxypropionic Acid (3-HP)
AU2012249390B2 (en) 2011-04-27 2017-03-30 Amyris, Inc. Methods for genomic modification
US8741603B2 (en) 2011-05-03 2014-06-03 Agrigenetics Inc. Enhancing spinosyn production with oxygen binding proteins
WO2012164565A1 (en) 2011-06-01 2012-12-06 Yeda Research And Development Co. Ltd. Compositions and methods for downregulating prokaryotic genes
US9752176B2 (en) 2011-06-15 2017-09-05 Ginkgo Bioworks, Inc. Methods for preparative in vitro cloning
CN103732744A (zh) 2011-06-15 2014-04-16 Gen9股份有限公司 制备性体外克隆的方法
US9506167B2 (en) 2011-07-29 2016-11-29 Ginkgo Bioworks, Inc. Methods and systems for cell state quantification
CA2850480A1 (en) 2011-09-30 2013-06-20 Mascoma Corporation Engineering microorganisms to increase ethanol production by metabolic redirection
US9422566B2 (en) 2011-10-12 2016-08-23 Sanofi Gene cluster for biosynthesis of griselimycin and methylgriselimycin
US8349587B2 (en) 2011-10-31 2013-01-08 Ginkgo Bioworks, Inc. Methods and systems for chemoautotrophic production of organic compounds
US8332160B1 (en) 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
US20130252240A1 (en) 2012-03-21 2013-09-26 Ventana Medical Systems, Inc. Cryoembedded cell concentrates, methods for making, and methods for using
AU2013266968B2 (en) 2012-05-25 2017-06-29 Emmanuelle CHARPENTIER Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription
WO2014011800A1 (en) 2012-07-10 2014-01-16 Pivot Bio, Inc. Methods for multipart, modular and scarless assembly of dna molecules
CN103031327B (zh) 2012-08-02 2015-11-25 华东理工大学 原核细菌光诱导基因表达系统及其调控基因表达的方法
WO2014025747A1 (en) 2012-08-07 2014-02-13 Codexis, Inc. Glucose and xylose co-utilization in e. coli
WO2014089436A1 (en) 2012-12-07 2014-06-12 Ginkgo Bioworks, Inc. Methods and systems for methylotrophic production of organic compounds
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
IL239344B2 (en) 2012-12-12 2024-06-01 Broad Inst Inc Systems engineering, methods and optimal guiding components for sequence manipulation
EP2840140B2 (en) 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US20140310830A1 (en) 2012-12-12 2014-10-16 Feng Zhang CRISPR-Cas Nickase Systems, Methods And Compositions For Sequence Manipulation in Eukaryotes
CN113355357B (zh) 2012-12-12 2024-12-03 布罗德研究所有限公司 对用于序列操纵的改进的系统、方法和酶组合物进行的工程化和优化
WO2014093655A2 (en) 2012-12-12 2014-06-19 The Broad Institute, Inc. Engineering and optimization of systems, methods and compositions for sequence manipulation with functional domains
US20140180660A1 (en) 2012-12-14 2014-06-26 Life Technologies Holdings Pte Limited Methods and systems for in silico design
US10102335B2 (en) 2012-12-19 2018-10-16 Virginia Commonwealth University Cost-optimized design analysis for rapid microbial prototyping
WO2014102782A1 (en) 2012-12-24 2014-07-03 Ramot At Tel-Aviv University Ltd Compositions and methods of killing bacteria
SI3044320T1 (sl) 2013-09-11 2020-07-31 Impossible Foods Inc. Sekrecija polipeptidov, ki vsebujejo hem
WO2015070193A1 (en) 2013-11-11 2015-05-14 Liu Oliver Compositions and methods for targeted gene disruption in prokaryotes
US9617569B2 (en) 2013-11-15 2017-04-11 Samsung Electronics Co., Ltd. Genetically engineered yeast cell producing lactate including acetaldehyde dehydrogenase, method of producing yeast cell, and method of producing lactate using the same
CN105992821A (zh) 2013-12-03 2016-10-05 诺维信公司 真菌基因文库的双分裂标记物整合
US11047232B2 (en) 2013-12-31 2021-06-29 Biota Technology, Inc Microbiome based systems, apparatus and methods for the exploration and production of hydrocarbons
US11028449B2 (en) 2013-12-31 2021-06-08 Biota Technology, Inc. Microbiome based systems, apparatus and methods for monitoring and controlling industrial processes and systems
US20170139078A1 (en) 2013-12-31 2017-05-18 Biota Technology, Inc. Microbiome based systems, apparatus and methods for the exploration and production of hydrocarbons
WO2015120403A1 (en) 2014-02-08 2015-08-13 The Regents Of The University Of Colorado, A Body Corporate Multiplexed linking pcr
EP3690044B1 (en) 2014-02-11 2024-01-10 The Regents of the University of Colorado, a body corporate Crispr enabled multiplexed genome engineering
WO2015148680A1 (en) 2014-03-25 2015-10-01 Ginkgo Bioworks, Inc. Methods and genetic systems for cell engineering
EP3143153A4 (en) 2014-05-16 2018-01-03 The University Of Georgia Research Foundation, Inc. Microbial approach for the production of 5-hydroxytryptophan
ES2781323T3 (es) 2014-06-23 2020-09-01 Regeneron Pharma Montaje de ADN mediado por nucleasas
WO2015199614A1 (en) 2014-06-27 2015-12-30 Nanyang Technological University Systems and methods for synthetic biology design and host cell simulation
US10815478B2 (en) 2014-11-05 2020-10-27 Illumina, Inc. Method of sequential tagmentation with transposase compositions for reduction of insertion bias
JP2018500037A (ja) 2014-12-31 2018-01-11 シンセティック ジェノミクス インコーポレーテッド 高効率なインビボゲノム編集のための組成物及び方法
US9650617B2 (en) 2015-01-28 2017-05-16 Pioneer Hi-Bred International. Inc. CRISPR hybrid DNA/RNA polynucleotides and methods of use
CA2981716C (en) 2015-04-10 2022-04-12 Feldan Bio Inc. Polypeptide-based shuttle agents for improving the transduction efficiency of polypeptide cargos to the cytosol of target eukaryotic cells, uses thereof, methods and kits relating to same
US10336999B2 (en) 2015-05-29 2019-07-02 Zymergen Inc. Compositions and methods for activating silent gene clusters
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
AU2016279077A1 (en) 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
WO2017070598A1 (en) 2015-10-23 2017-04-27 Caribou Biosciences, Inc. Engineered crispr class 2 cross-type nucleic-acid targeting nucleic acids
BR112018011503A2 (pt) 2015-12-07 2018-12-04 Zymergen Inc promotores da corynebacterium glutamicum
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11151497B2 (en) 2016-04-27 2021-10-19 Zymergen Inc. Microbial strain design system and methods for improved large-scale production of engineered nucleotide sequences
KR20190090081A (ko) 2015-12-07 2019-07-31 지머젠 인코포레이티드 Htp 게놈 공학 플랫폼에 의한 미생물 균주 개량
WO2017215790A1 (en) 2016-06-17 2017-12-21 National Hellenic Research Foundation Systems for recombinant protein production
AU2017280353B2 (en) 2016-06-24 2021-11-11 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
EP3478845A4 (en) 2016-06-30 2019-07-31 Zymergen, Inc. METHOD OF GENERATING A GLUCOSE PERMEASE LIBRARY AND USES THEREOF
US10544390B2 (en) * 2016-06-30 2020-01-28 Zymergen Inc. Methods for generating a bacterial hemoglobin library and uses thereof
KR20190027843A (ko) 2016-07-05 2019-03-15 지머젠 인코포레이티드 Rna 데그라도솜 단백질 복합체의 유전자 교란
US20190330659A1 (en) 2016-07-15 2019-10-31 Zymergen Inc. Scarless dna assembly and genome editing using crispr/cpf1 and dna ligase
WO2017037304A2 (en) 2016-07-28 2017-03-09 Dsm Ip Assets B.V. An assembly system for a eukaryotic cell
EP3491003A4 (en) 2016-07-28 2020-03-25 Ginkgo Bioworks Inc. DEVICES AND METHODS FOR NUCLEIC ACID MANIPULATION
WO2018071672A1 (en) 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992003546A1 (en) * 1990-08-23 1992-03-05 Exogene Corporation Enhancement of production of native products in corynebacterium by expression of cloned bacterial hemoglobin
US20060269975A1 (en) * 1999-07-16 2006-11-30 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding proteins involved in DNA replication, protein synthesis, and pathogenesis
WO2006028063A1 (ja) * 2004-09-09 2006-03-16 Research Institute Of Innovative Technology For The Earth プロモーター機能を有するdna断片

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