JP2019514376A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2019514376A5 JP2019514376A5 JP2018555963A JP2018555963A JP2019514376A5 JP 2019514376 A5 JP2019514376 A5 JP 2019514376A5 JP 2018555963 A JP2018555963 A JP 2018555963A JP 2018555963 A JP2018555963 A JP 2018555963A JP 2019514376 A5 JP2019514376 A5 JP 2019514376A5
- Authority
- JP
- Japan
- Prior art keywords
- molecule
- nucleic acid
- funa
- vector
- target
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 108020004707 nucleic acids Proteins 0.000 claims 13
- 102000039446 nucleic acids Human genes 0.000 claims 13
- 150000007523 nucleic acids Chemical class 0.000 claims 12
- 238000000034 method Methods 0.000 claims 8
- 229920001184 polypeptide Polymers 0.000 claims 6
- 102000004196 processed proteins & peptides Human genes 0.000 claims 6
- 108090000765 processed proteins & peptides Proteins 0.000 claims 6
- 241001517610 Funa Species 0.000 claims 3
- 230000006801 homologous recombination Effects 0.000 claims 2
- 238000002744 homologous recombination Methods 0.000 claims 2
- 108020004414 DNA Proteins 0.000 claims 1
- 102000053602 DNA Human genes 0.000 claims 1
- 241001465754 Metazoa Species 0.000 claims 1
- 230000000295 complement effect Effects 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 claims 1
- 125000006850 spacer group Chemical group 0.000 claims 1
- -1 spacer nucleic acid Chemical class 0.000 claims 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2022187824A JP2023018093A (ja) | 2016-04-29 | 2022-11-24 | 標的核酸の改変のための改善された方法 |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16167774 | 2016-04-29 | ||
| EP16167773.7 | 2016-04-29 | ||
| EP16167774.5 | 2016-04-29 | ||
| EP16167773 | 2016-04-29 | ||
| EP17156018.8 | 2017-02-14 | ||
| EP17156018 | 2017-02-14 | ||
| PCT/EP2017/059331 WO2017186550A1 (en) | 2016-04-29 | 2017-04-20 | Improved methods for modification of target nucleic acids |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022187824A Division JP2023018093A (ja) | 2016-04-29 | 2022-11-24 | 標的核酸の改変のための改善された方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2019514376A JP2019514376A (ja) | 2019-06-06 |
| JP2019514376A5 true JP2019514376A5 (OSRAM) | 2020-06-11 |
| JP7184648B2 JP7184648B2 (ja) | 2022-12-06 |
Family
ID=58640844
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018555963A Active JP7184648B2 (ja) | 2016-04-29 | 2017-04-20 | 標的核酸の改変のための改善された方法 |
| JP2022187824A Pending JP2023018093A (ja) | 2016-04-29 | 2022-11-24 | 標的核酸の改変のための改善された方法 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2022187824A Pending JP2023018093A (ja) | 2016-04-29 | 2022-11-24 | 標的核酸の改変のための改善された方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (5) | US11608499B2 (OSRAM) |
| EP (5) | EP3448990B1 (OSRAM) |
| JP (2) | JP7184648B2 (OSRAM) |
| KR (3) | KR102370675B1 (OSRAM) |
| CN (1) | CN109072207B (OSRAM) |
| AU (5) | AU2017256517B2 (OSRAM) |
| CA (1) | CA3022475A1 (OSRAM) |
| CL (1) | CL2018003074A1 (OSRAM) |
| DK (1) | DK3448990T5 (OSRAM) |
| IL (1) | IL262409B2 (OSRAM) |
| MX (2) | MX388092B (OSRAM) |
| WO (1) | WO2017186550A1 (OSRAM) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3613852A3 (en) | 2011-07-22 | 2020-04-22 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US20180250424A1 (en) | 2014-10-10 | 2018-09-06 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| EP3215617B1 (en) | 2014-11-07 | 2024-05-08 | Editas Medicine, Inc. | Systems for improving crispr/cas-mediated genome-editing |
| WO2017053879A1 (en) | 2015-09-24 | 2017-03-30 | Editas Medicine, Inc. | Use of exonucleases to improve crispr/cas-mediated genome editing |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| WO2017165826A1 (en) | 2016-03-25 | 2017-09-28 | Editas Medicine, Inc. | Genome editing systems comprising repair-modulating enzyme molecules and methods of their use |
| US11236313B2 (en) | 2016-04-13 | 2022-02-01 | Editas Medicine, Inc. | Cas9 fusion molecules, gene editing systems, and methods of use thereof |
| CA3025828A1 (en) * | 2016-06-01 | 2017-12-07 | Kws Saat Se | Hybrid nucleic acid sequences for genome engineering |
| GB2568182A (en) | 2016-08-03 | 2019-05-08 | Harvard College | Adenosine nucleobase editors and uses thereof |
| WO2018031683A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| US20190330620A1 (en) * | 2016-10-14 | 2019-10-31 | Emendobio Inc. | Rna compositions for genome editing |
| GB2573062A (en) | 2016-10-14 | 2019-10-23 | Harvard College | AAV delivery of nucleobase editors |
| KR20190089175A (ko) * | 2016-11-18 | 2019-07-30 | 진에딧 인코포레이티드 | 표적 핵산 변형을 위한 조성물 및 방법 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EA201991809A1 (ru) * | 2017-01-30 | 2020-02-05 | КВС ЗААТ СЕ & КО. КГаА | Сцепление матрицы для репарации с эндонуклеазами для геномной инженерии |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165631A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Cancer vaccine |
| CN110914310A (zh) | 2017-03-10 | 2020-03-24 | 哈佛大学的校长及成员们 | 胞嘧啶至鸟嘌呤碱基编辑器 |
| US11268082B2 (en) | 2017-03-23 | 2022-03-08 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable DNA binding proteins |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| MX2019015188A (es) | 2017-06-15 | 2020-08-03 | Univ California | Inserciones de adn no virales orientadas. |
| WO2019014564A1 (en) | 2017-07-14 | 2019-01-17 | Editas Medicine, Inc. | SYSTEMS AND METHODS OF TARGETED INTEGRATION AND GENOME EDITING AND DETECTION THEREOF WITH INTEGRATED PRIMING SITES |
| JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| AU2018355587B2 (en) | 2017-10-27 | 2023-02-02 | The Regents Of The University Of California | Targeted replacement of endogenous T cell receptors |
| US12406749B2 (en) | 2017-12-15 | 2025-09-02 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US11268092B2 (en) | 2018-01-12 | 2022-03-08 | GenEdit, Inc. | Structure-engineered guide RNA |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| WO2020047124A1 (en) | 2018-08-28 | 2020-03-05 | Flagship Pioneering, Inc. | Methods and compositions for modulating a genome |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| KR102298105B1 (ko) | 2019-01-08 | 2021-09-03 | 주식회사 엘지에너지솔루션 | 스웰링 게이지를 구비한 배터리 모듈 및 이를 포함하는 배터리 팩 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| AU2020240109A1 (en) | 2019-03-19 | 2021-09-30 | President And Fellows Of Harvard College | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| KR20220150328A (ko) | 2020-03-04 | 2022-11-10 | 바스프 에스이 | 낮은 내지 중간 발현을 부여하는 구성적 박테리아 프로모터를 생산하는 방법 |
| IL296095A (en) | 2020-03-04 | 2022-11-01 | Flagship Pioneering Innovations Vi Llc | Improved methods and compositions for genome modulation |
| US20230139192A1 (en) | 2020-03-04 | 2023-05-04 | Basf Se | Shuttle vector for expression in e. coli and bacilli |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| CN112111505B (zh) * | 2020-09-17 | 2022-03-15 | 江南大学 | 一种氧化葡萄糖酸杆菌中基因敲除的方法 |
| WO2022251179A1 (en) * | 2021-05-26 | 2022-12-01 | Inscripta, Inc. | Crispr editing in diploid genomes |
| US20240263173A1 (en) * | 2021-08-11 | 2024-08-08 | The Board Of Trustees Of The Leland Stanford Junior University | High-throughput precision genome editing in human cells |
| EP4416292A2 (en) | 2021-10-14 | 2024-08-21 | Arsenal Biosciences, Inc. | Immune cells having co-expressed shrnas and logic gate systems |
| WO2023114872A2 (en) * | 2021-12-14 | 2023-06-22 | The Broad Institute, Inc. | Reprogrammable fanzor polynucleotides and uses thereof |
| KR20240153594A (ko) | 2022-03-01 | 2024-10-23 | 바스프 아그리컬쳐럴 솔루션즈 시드 유에스 엘엘씨 | Cas12a 니카제 |
| CN114774420B (zh) * | 2022-04-27 | 2023-09-22 | 扬州大学 | 一种水稻增强子及基于CRISPR/Cas9技术评估水稻增强子活性的方法 |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DK0733059T3 (da) | 1993-12-09 | 2000-10-16 | Univ Jefferson | Forbindelser og fremgangsmåder til site-rettede mutationer i eukaryote celler |
| US6027945A (en) | 1997-01-21 | 2000-02-22 | Promega Corporation | Methods of isolating biological target materials using silica magnetic particles |
| US6555732B1 (en) | 1998-09-14 | 2003-04-29 | Pioneer Hi-Bred International, Inc. | Rac-like genes and methods of use |
| EP1152058A1 (en) * | 2000-05-03 | 2001-11-07 | Institut Curie | Methods and compositions for effecting homologous recombination |
| EP2336362B1 (en) | 2005-08-26 | 2018-09-19 | DuPont Nutrition Biosciences ApS | Use of crispr associated genes (cas) |
| NZ712727A (en) * | 2013-03-14 | 2017-05-26 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| ES2767318T3 (es) | 2013-06-17 | 2020-06-17 | Broad Inst Inc | Suministro, modificación y optimización de sistemas, métodos y composiciones para generar modelos y actuar sobre enfermedades y trastornos de células posmitóticas |
| US10563225B2 (en) * | 2013-07-26 | 2020-02-18 | President And Fellows Of Harvard College | Genome engineering |
| CA2920591A1 (en) | 2013-08-12 | 2015-02-19 | BASF Agro B.V. | Plants having increased tolerance to herbicides |
| MD4770B1 (ro) | 2013-12-18 | 2021-10-31 | BASF Agro B.V. | Plante cu toleranţă sporită la erbicide |
| WO2015191693A2 (en) | 2014-06-10 | 2015-12-17 | Massachusetts Institute Of Technology | Method for gene editing |
| WO2016065364A1 (en) * | 2014-10-24 | 2016-04-28 | Life Technologies Corporation | Compositions and methods for enhancing homologous recombination |
| US11535871B2 (en) | 2015-05-14 | 2022-12-27 | University Of Southern California | Optimized gene editing utilizing a recombinant endonuclease system |
| WO2017024047A1 (en) * | 2015-08-03 | 2017-02-09 | Emendobio Inc. | Compositions and methods for increasing nuclease induced recombination rate in cells |
| CA2998287A1 (en) * | 2015-09-24 | 2017-04-20 | Crispr Therapeutics Ag | Novel family of rna-programmable endonucleases and their uses in genome editing and other applications |
| WO2017059241A1 (en) * | 2015-10-02 | 2017-04-06 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Lentiviral protein delivery system for rna-guided genome editing |
| CN106811479B (zh) | 2015-11-30 | 2019-10-25 | 中国农业科学院作物科学研究所 | 利用CRISPR/Cas9系统定点修饰ALS基因获得抗除草剂水稻的系统及其应用 |
| US20190062734A1 (en) * | 2016-04-13 | 2019-02-28 | Editas Medicine, Inc. | Grna fusion molecules, gene editing systems, and methods of use thereof |
| KR20190089175A (ko) | 2016-11-18 | 2019-07-30 | 진에딧 인코포레이티드 | 표적 핵산 변형을 위한 조성물 및 방법 |
-
2017
- 2017-04-20 WO PCT/EP2017/059331 patent/WO2017186550A1/en not_active Ceased
- 2017-04-20 EP EP17720040.9A patent/EP3448990B1/en not_active Revoked
- 2017-04-20 CN CN201780025990.6A patent/CN109072207B/zh active Active
- 2017-04-20 EP EP21162462.2A patent/EP3868880A1/en not_active Withdrawn
- 2017-04-20 EP EP22193652.9A patent/EP4166660A1/en not_active Withdrawn
- 2017-04-20 CA CA3022475A patent/CA3022475A1/en active Pending
- 2017-04-20 MX MX2018013256A patent/MX388092B/es unknown
- 2017-04-20 KR KR1020187030717A patent/KR102370675B1/ko active Active
- 2017-04-20 KR KR1020237007132A patent/KR20230038804A/ko not_active Ceased
- 2017-04-20 DK DK17720040.9T patent/DK3448990T5/da active
- 2017-04-20 EP EP22193654.5A patent/EP4166661A1/en active Pending
- 2017-04-20 KR KR1020227006922A patent/KR102506185B1/ko active Active
- 2017-04-20 EP EP22193659.4A patent/EP4166662A1/en not_active Withdrawn
- 2017-04-20 AU AU2017256517A patent/AU2017256517B2/en active Active
- 2017-04-20 JP JP2018555963A patent/JP7184648B2/ja active Active
- 2017-04-20 US US16/097,300 patent/US11608499B2/en active Active
-
2018
- 2018-10-16 IL IL262409A patent/IL262409B2/en unknown
- 2018-10-29 CL CL2018003074A patent/CL2018003074A1/es unknown
- 2018-10-29 MX MX2021009235A patent/MX2021009235A/es unknown
-
2022
- 2022-09-16 US US17/933,005 patent/US20230183686A1/en active Pending
- 2022-09-16 US US17/933,023 patent/US20230107997A1/en active Pending
- 2022-09-16 US US17/933,003 patent/US20230024869A1/en active Pending
- 2022-09-16 US US17/933,015 patent/US20230049124A1/en active Pending
- 2022-11-04 AU AU2022100158A patent/AU2022100158A4/en not_active Expired
- 2022-11-04 AU AU2022100155A patent/AU2022100155A4/en not_active Expired
- 2022-11-04 AU AU2022100157A patent/AU2022100157A4/en not_active Expired
- 2022-11-14 AU AU2022100170A patent/AU2022100170A4/en not_active Expired
- 2022-11-24 JP JP2022187824A patent/JP2023018093A/ja active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2019514376A5 (OSRAM) | ||
| BR112017022852A2 (pt) | polipeptídeo recombinante com atividade pesticida, composição, molécula de ácido nucleico recombinante, ácido nucleico recombinante, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo, planta, semente transgênica, método para proteger uma planta e método para aumentar o rendimento em uma planta | |
| CN109072207A (zh) | 用于修饰靶核酸的改进方法 | |
| BR122023016256B8 (pt) | Polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, método de obtenção de uma planta transgênica ou célula de planta transgênica, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas e uso do polipeptídeo | |
| BR112017025929A2 (pt) | genes pesticidas e métodos de uso | |
| RU2017134741A (ru) | Полипептиды транспозазы и их применение | |
| BR112016005543A2 (pt) | construto de dna, polinucleotídeo isolado, polinucleotídeo isolado de dna, cassete de expressão, planta transgênica, semente, planta da progênie, célula hospedeira, polipeptídeo pip-72 recombinante, composição, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto, método para controlar uma infestação de insetos em uma planta transgênica e fornecer o manejo da resistência, método para identificar em uma amostra biológica uma sequência de nucleotídeos que codifica o polipeptídeo pip-72, método para identificar em uma amostra o polipeptídeo pip-72 | |
| CL2010001436A1 (es) | Delta endotoxinas con actividade plaguicida, composiciones y metodos para conferir resisitencia a pestes a plantas, moleculas de acidos nucleicos que codifican dichas delta endotoxinas, vectores, celulas huesped, anticuerpos, y usos. | |
| MX2022000189A (es) | Genes pesticidas y metodos de uso. | |
| PH12018501338A1 (en) | Pesticidal genes and methods of use | |
| WO2018140859A3 (en) | Pesticidal genes and methods of use | |
| BR112019012339A2 (pt) | polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão | |
| Davidson et al. | Yersinia pseudotuberculosis: cultivation, storage, and methods for introducing DNA | |
| BR112017022383A2 (pt) | polipeptídeo recombinante, composição, molécula de ácido nucleico recombinante, ácido nucleico recombinante, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo, planta, semente transgênica da planta, método para proteger uma planta, método para aumentar o rendimento em uma planta | |
| Cao et al. | An improved transient expression system using Arabidopsis protoplasts | |
| BR112017013633A2 (pt) | polipeptídeo recombinante, molécula de ácido nucleico recombinante, composição, célula hospedeira, construto de dna, vetor, método para controlar uma população de pragas, método para produzir um polipeptídeo com atividade pesticida, planta, semente transgênica, método para proteger uma planta de uma praga de insetos, método para aumentar o rendimento em uma planta | |
| PH12021550691A1 (en) | Pesticidal genes and methods of use | |
| RU2626590C2 (ru) | Генетическая конструкция для экспрессии генов в клетках насекомого polypedilum vanderplanki | |
| RU2018145686A (ru) | Генотерапевтический ДНК-вектор на основе генотерапевтического ДНК-вектора VTvaf17, несущий целевой ген, выбранный из группы генов SHH, CTNNB1, NOG, WNT7A для повышения уровня экспрессии этих целевых генов, способ его получения и применения, штамм Escherichia coli SCS110-AF/VTvaf17-SHH или Escherichia coli SCS110-AF/VTvaf17-CTNNB1 или Escherichia coli SCS110-AF/VTvaf17-NOG или Escherichia coli SCS110-AF/VTvaf17-WNT7A, несущий генотерапевтический ДНК-вектор, способ его получения, способ производства в промышленных масштабах генотерапевтического ДНК-вектора | |
| CN104131019A (zh) | 含MyoDI基因启动子片段的重组质粒的构建方法 | |
| CN119751611A (zh) | 小立碗藓复制起始位点片段及鉴定方法与应用 | |
| RU2018143698A (ru) | Генотерапевтический ДНК-вектор на основе генотерапевтического ДНК-вектора VTvaf17, несущий целевой ген, выбранный из группы генов SOD1, SOD2, SOD3, CAT для повышения уровня экспрессии этих целевых генов, способ его получения и применения, штамм Escherichia coli SCS110-AF/VTvaf17-SOD1 или Escherichia coli SCS110-AF/VTvaf17-SOD2 или Escherichia coli SCS110-AF/VTvaf17-SOD3 или Escherichia coli SCS110-AF/VTvaf17-CAT, несущий генотерапевтический ДНК-вектор, способ его получения, способ производства в промышленных масштабах генотерапевтического ДНК-вектора | |
| CN103849640A (zh) | 一种寡核苷酸和可消除质粒共转化用于大肠杆菌必需基因点突变的方法 | |
| AR111369A1 (es) | Genes pesticidas y métodos de uso | |
| AR113099A1 (es) | Genes pesticidas y métodos de uso |