JP2019513973A - タンパク質凝集体のホログラフィ特徴付け - Google Patents
タンパク質凝集体のホログラフィ特徴付け Download PDFInfo
- Publication number
- JP2019513973A JP2019513973A JP2018541149A JP2018541149A JP2019513973A JP 2019513973 A JP2019513973 A JP 2019513973A JP 2018541149 A JP2018541149 A JP 2018541149A JP 2018541149 A JP2018541149 A JP 2018541149A JP 2019513973 A JP2019513973 A JP 2019513973A
- Authority
- JP
- Japan
- Prior art keywords
- protein
- protein aggregates
- aggregates
- refractive index
- radius
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1468—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle
- G01N15/147—Electro-optical investigation, e.g. flow cytometers with spatial resolution of the texture or inner structure of the particle the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N15/0211—Investigating a scatter or diffraction pattern
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N15/0227—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging using imaging, e.g. a projected image of suspension; using holography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N2015/0038—Investigating nanoparticles
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N2015/0042—Investigating dispersion of solids
- G01N2015/0053—Investigating dispersion of solids in liquids, e.g. trouble
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N15/0211—Investigating a scatter or diffraction pattern
- G01N2015/0222—Investigating a scatter or diffraction pattern from dynamic light scattering, e.g. photon correlation spectroscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/02—Investigating particle size or size distribution
- G01N15/0205—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging
- G01N2015/0233—Investigating particle size or size distribution by optical means, e.g. by light scattering, diffraction, holography or imaging using holography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1434—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement
- G01N2015/1454—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its optical arrangement using phase shift or interference, e.g. for improving contrast
Abstract
Description
ホログラフィ特徴付け器具の較正
ホログラフィ特徴付けの動作範囲
ホログラフィによる形態測定
実施例
材料の調製
正確さ及び精密性の証明
肉眼で見えないインスリン凝集物の特徴付け
肉眼で見えないBSA凝集体の特徴付け
凝集体形態の役割
マイクロフロー画像化
動的光散乱
タンパク質凝集体からのシリコン球のホログラフィによる差別化
シリコン球のホログラフィによる特徴付け
シリコン球の差別化による検出
実施例の結果の考察
凝集体サイズと屈折率との間の反相関関係
これから
差別化
コンピュータ実施
Claims (21)
- 複数のタンパク質凝集体のサンプルを特徴付ける方法であって、
前記サンプルをホログラフィ顕微鏡の観察分量を通して流す工程と、
前記観察分量内の第1のセットのタンパク質凝集体のホログラフィビデオ顕微鏡検査に基づいて、第1の時間に第1のセットのホログラムを生成する工程と、
前記第1のセットのタンパク質凝集体の前記タンパク質凝集体の各々を球体として特徴付ける工程と、
前記第1のセットのタンパク質凝集体の特徴付けられた球体に関する屈折率及び半径を決定する工程と、を備えることを特徴とする方法。 - 前記観察分量内の第2のセットのタンパク質凝集体のホログラフィビデオ顕微鏡検査に基づいて、第2の時間に第2のセットのホログラムを生成する工程と、
前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の各々を球体として特徴付ける工程と、
前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の各々に関する屈折率及び半径を決定する工程と、をさらに備える、請求項1に記載の方法。 - 前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の各々の特徴付け工程は、前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の各々を、フラクタクル次元Dのフラクタルクラスタとしてモデル化する工程をさらに備え、
- さらにタンパク質の前記分量分率は反射率がn0であり、残りの分量は、屈折率がnmの流体媒体であり前記タンパク質凝集体の見かけの屈折率npをもたらす、請求項2に記載の方法。
- 前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の形態を、アンサンブル平均のフラクタル次元を推定する工程に基づいて特徴付ける工程をさらに備える、請求項3に記載の方法。
- 前記アンサンブル平均のフラクタル次元を推定する工程は、
- 前記第1の粒子の屈折率及び半径と前記第2の粒子の屈折率及び半径との比較に基づいて複数の粒子の合成を監視する工程をさらに備える、請求項1に記載の方法。
- 前記サンプルを流す工程は、100μms-1までの速度であることをさらに備える、請求項1に記載の方法。
- 前記第1のセットのホログラムを生成する前に、塩を添加する工程をさらに備える、請求項1に記載の方法。
- 前記屈折率及び前記半径を決定する工程は、ローレンツ・ミー理論の適用を備える、請求項1に記載の方法。
- 前記複数の粒子の合成が完了したかどうかを判定する工程をさらに備える、請求項1に記載の方法。
- 前記第1のセットのタンパク質凝集体の前記タンパク質凝集体の前記屈折率及び前記半径を決定する工程、並びに前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の前記屈折率及び前記半径を決定する工程は、それぞれ前記第1のセットのタンパク質凝集体の前記タンパク質凝集体の屈折率及び半径の確率密度を決定する工程、並びに前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の屈折率及び半径の確率密度を決定する工程を備える、請求項1に記載の方法。
- 前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の両方に少なくとも1つのタンパク質凝集体が存在し、さらに、前記少なくとも一方のタンパク質凝集体の軌道が決定される、請求項1に記載の方法。
- 複数のタンパク質凝集体を特徴付ける方法であって、
前記複数のタンパク質凝集体のタンパク質凝集体のホログラムを生成し、各ホログラムは、前記複数のタンパク質凝集体のタンパク質凝集体PNのホログラフィビデオ顕微鏡検査に基づいて時間TNに生成される工程と、
前記タンパク質凝集体を球体として特徴付ける工程と、
前記タンパク質凝集体PNの前記屈折率及び前記半径を時間TNに決定する工程と、
前記複数のタンパク質凝集体の経時的な変化を、前記粒子の前記決定された屈折率及び半径に基づいて特徴付ける工程と、を備えることを特徴とする方法。 - 前記複数のタンパク質凝集体の凝集を、前記決定された屈折率間及び半径間の比較に基づき監視する工程をさらに含む、請求項14に記載の方法。
- 前記屈折率及び前記半径を決定する工程は、ローレンツ・ミー理論の適用を備える、請求項14に記載の方法。
- 前記複数のタンパク質凝集体を、ホログラフィビデオ顕微鏡検査システムを通して流す工程をさらに備える、請求項14に記載の方法。
- 前記モデル化する工程は、前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の各々を、フラクタル次元Dのフラクタルクラスタとしてモデル化する工程をさらに含み、
- さらに前記タンパク質の分量分率は屈折率がn0であり、残りの分量は屈折率がnmの流体媒質であり前記タンパク質凝集体の見かけの屈折率npをもたらし、方法は、前記第1のセットのタンパク質凝集体及び前記第2のセットのタンパク質凝集体の形態を、アンサンブル平均のフラクタル次元を推定する工程に基づいて特徴付ける工程をさらに備える、請求項18に記載の方法。
- 前記アンサンブル平均のフラクタル次元を推定する工程は、
- 複数のタンパク質凝集体を特徴付けるためのコンピュータ実行機械であって、
プロセッサと、
コヒーレント光と、観察分量を有する検査サンプル台と、対物レンズと、画像収集装置とを含み、前記プロセッサと通信するホログラフィ顕微鏡と、
前記プロセッサに動作可能に接続されかつコンピュータコードを有する有形のコンピュータ可読媒体と、を備え、前記コンピュータコードは、
前記ホログラフィ顕微鏡の観察分量を通るサンプルの流れを制御し、
前記観察分量内の第1のセットのタンパク質凝集体のホログラフィビデオ顕微鏡検査に基づいて第1の時間に第1のセットのホログラムを受け取り、
前記第1のセットのタンパク質凝集体の前記タンパク質凝集体の各々を球体としてモデル化し、
前記第1のセットのタンパク質凝集体の前記タンパク質凝集体の各球体に関する前記屈折率及び前記半径を決定し、
前記観察分量内の第2のセットのタンパク質凝集体のホログラフィビデオ顕微鏡検査に基づいて第2の時間に第2のセットのホログラムを受け取り、
前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の各々を球体としてモデル化し、
前記第2のセットのタンパク質凝集体の前記タンパク質凝集体の各球体に関する前記屈折率及び前記半径を決定する、ように構成されていることを特徴とするコンピュータ実行機械。
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662292842P | 2016-02-08 | 2016-02-08 | |
US62/292,842 | 2016-02-08 | ||
PCT/US2017/016857 WO2017139279A2 (en) | 2016-02-08 | 2017-02-07 | Holographic characterization of protein aggregates |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2019513973A true JP2019513973A (ja) | 2019-05-30 |
JP7130242B2 JP7130242B2 (ja) | 2022-09-05 |
Family
ID=59564000
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP2018541149A Active JP7130242B2 (ja) | 2016-02-08 | 2017-02-07 | タンパク質凝集体のホログラフィ特徴付け |
Country Status (7)
Country | Link |
---|---|
US (2) | US11385157B2 (ja) |
EP (1) | EP3414517B1 (ja) |
JP (1) | JP7130242B2 (ja) |
DK (1) | DK3414517T3 (ja) |
ES (1) | ES2901608T3 (ja) |
TW (1) | TWI731030B (ja) |
WO (1) | WO2017139279A2 (ja) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010101671A1 (en) | 2009-01-16 | 2010-09-10 | New York University | Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy |
ES2913524T3 (es) | 2014-11-12 | 2022-06-02 | Univ New York | Huellas coloidales para materiales blandos usando caracterización holográfica total |
EP3414517B1 (en) * | 2016-02-08 | 2021-10-06 | New York University | Holographic characterization of protein aggregates |
JP2022510388A (ja) * | 2018-12-04 | 2022-01-26 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | タンパク質結晶の高コントラストイメージングのための携帯型uvホログラフィック顕微鏡 |
US11543338B2 (en) | 2019-10-25 | 2023-01-03 | New York University | Holographic characterization of irregular particles |
US11948302B2 (en) | 2020-03-09 | 2024-04-02 | New York University | Automated holographic video microscopy assay |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011502256A (ja) * | 2007-10-30 | 2011-01-20 | ニュー・ヨーク・ユニヴァーシティ | ホログラフィックビデオ顕微鏡による粒子の追跡および特徴付け |
CN102411298A (zh) * | 2011-11-30 | 2012-04-11 | 昆明理工大学 | 一种三维实时超分辨数字全息记录方法 |
WO2013019640A1 (en) * | 2011-07-29 | 2013-02-07 | The Regents Of The University Of California | Lensfree holographic microscopy using wetting films |
JP2014525583A (ja) * | 2011-08-29 | 2014-09-29 | アムジェン インコーポレイテッド | 流体中の非溶解粒子の非破壊的検出のための方法および装置 |
WO2015200512A1 (en) * | 2014-06-25 | 2015-12-30 | New York University | In-line particle characterization |
Family Cites Families (153)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3069654A (en) | 1960-03-25 | 1962-12-18 | Paul V C Hough | Method and means for recognizing complex patterns |
US3551018A (en) | 1969-05-01 | 1970-12-29 | Karl A Stetson | Total internal reflection holography |
JPS5596976A (en) | 1979-01-17 | 1980-07-23 | Ricoh Co Ltd | Hologram recorder |
FR2476839A1 (fr) | 1980-02-25 | 1981-08-28 | Anvar | Procede de determination du diametre d'une particule en suspension dans un fluide par utilisation d'un rayonnement electromagnetique coherent |
JPS58155636A (ja) | 1982-03-12 | 1983-09-16 | Hitachi Ltd | ホログラフイ電子顕微鏡 |
DE3303876A1 (de) | 1983-02-05 | 1984-08-09 | M.A.N. Maschinenfabrik Augsburg-Nürnberg AG, 8000 München | Holografisches differenzverfahren |
US4540285A (en) | 1983-05-16 | 1985-09-10 | The United States Of America As Represented By The United States Department Of Energy | Photothermal method of determining calorific properties of coal |
US4740079A (en) | 1984-10-29 | 1988-04-26 | Hitachi, Ltd. | Method of and apparatus for detecting foreign substances |
GB8703228D0 (en) | 1987-02-12 | 1987-03-18 | Hugle W B | Positional detection of objects |
US4986659A (en) | 1988-02-29 | 1991-01-22 | Aerometrics, Inc. | Method for measuring the size and velocity of spherical particles using the phase and intensity of scattered light |
JP2776862B2 (ja) | 1989-01-13 | 1998-07-16 | 株式会社日立製作所 | 反射電子線ホログラフイー装置 |
JPH03251388A (ja) | 1990-02-28 | 1991-11-08 | Sony Corp | ロボット |
JPH03251888A (ja) | 1990-02-28 | 1991-11-11 | Shimadzu Corp | ホログラフイカメラ |
US5095207A (en) | 1991-01-07 | 1992-03-10 | University Of Wisconsin - Milwaukee | Method of three-dimensional atomic imaging |
US5146086A (en) | 1991-03-19 | 1992-09-08 | Chevron Research And Technology Company | Method and apparatus for imaging porous media and method for fabricating novel optical materials |
GB9203239D0 (en) | 1992-02-14 | 1992-04-01 | Imperial College | Holographic imaging |
US5373727A (en) | 1993-04-16 | 1994-12-20 | New Mexico Tech Research Foundation | Miniporopermeameter |
US6797942B2 (en) | 2001-09-13 | 2004-09-28 | University Of Chicago | Apparatus and process for the lateral deflection and separation of flowing particles by a static array of optical tweezers |
US5995153A (en) | 1995-11-02 | 1999-11-30 | Prime Image, Inc. | Video processing system with real time program duration compression and expansion |
IL126544A (en) | 1996-04-25 | 2004-08-31 | Genicon Sciences Inc | Test for component detection using detectable particles in diffused light |
US7041510B2 (en) | 1996-04-25 | 2006-05-09 | Bioarray Solutions Ltd. | System and method for programmable illumination pattern generation |
US20040004717A1 (en) | 1996-11-13 | 2004-01-08 | Reed Wayne F. | Automatic mixing and dilution methods and apparatus for online characterization of equilibrium and non-equilibrium properties of solutions containing polymers and/or colloids |
US6480285B1 (en) | 1997-01-28 | 2002-11-12 | Zetetic Institute | Multiple layer confocal interference microscopy using wavenumber domain reflectometry and background amplitude reduction and compensation |
US5939716A (en) | 1997-04-02 | 1999-08-17 | Sandia Corporation | Three-dimensional light trap for reflective particles |
US5880841A (en) | 1997-09-08 | 1999-03-09 | Erim International, Inc. | Method and apparatus for three-dimensional imaging using laser illumination interferometry |
US6055106A (en) | 1998-02-03 | 2000-04-25 | Arch Development Corporation | Apparatus for applying optical gradient forces |
US7133203B2 (en) | 1998-02-03 | 2006-11-07 | Arch Development Corporation | Apparatus for applying optical gradient forces |
GB9810865D0 (en) | 1998-05-20 | 1998-07-22 | Zeneca Ltd | Nucleic acid sequence identification |
US6097488A (en) | 1998-06-22 | 2000-08-01 | Princeton University | Method and apparatus for measuring micro structures, anisotropy and birefringence in polymers using laser scattered light |
JP2001034148A (ja) | 1999-07-26 | 2001-02-09 | Nippon Telegr & Teleph Corp <Ntt> | 立体映像表示方法および装置 |
US6281994B1 (en) | 1998-12-22 | 2001-08-28 | Nippon Telegraph And Telephone Corporation | Method and apparatus for three-dimensional holographic display suitable for video image display |
JP2000225302A (ja) | 1999-02-04 | 2000-08-15 | Mitsubishi Chemicals Corp | 粒度分布の特徴量の推定方法、粒子の製造方法及びこれらに用いる装置 |
AU4328000A (en) | 1999-03-31 | 2000-10-16 | Verizon Laboratories Inc. | Techniques for performing a data query in a computer system |
US6608716B1 (en) * | 1999-05-17 | 2003-08-19 | New Mexico State University Technology Transfer Corporation | Optical enhancement with nanoparticles and microcavities |
DE19954933A1 (de) | 1999-11-10 | 2001-05-17 | Zeiss Carl Jena Gmbh | Anordnung zur Einkopplung einer optischen Pinzette und/oder eines Bearbeitungsstrahles in ein Mikroskop |
JP2003515405A (ja) | 1999-12-06 | 2003-05-07 | エモリー ユニバーシティ | 機能の磁気共鳴画像化データ分析役務を提供するためのシステムと方法 |
WO2001081977A2 (en) | 2000-04-25 | 2001-11-01 | Silicon Valley Group, Inc. | Optical reduction system with control of illumination polarization |
TW531661B (en) | 2000-10-06 | 2003-05-11 | Arch Dev Corp | Method of controllably filling an array of small particles, method of controllably manipulating an array of optical traps, and apparatus for controllably manipulating an array of optical traps |
US20030007894A1 (en) | 2001-04-27 | 2003-01-09 | Genoptix | Methods and apparatus for use of optical forces for identification, characterization and/or sorting of particles |
AU2002307550A1 (en) * | 2001-04-25 | 2002-11-05 | New Mexico State University Technology Transfer Corporation | Plasmonic nanophotonics methods, materials, and apparatuses |
CA2451166C (fr) | 2001-06-29 | 2011-02-22 | Universite Libre De Bruxelles | Procede et dispositif destines a l'obtention par microscopie d'images en trois dimensions d'un echantillon |
US7338168B2 (en) | 2001-07-06 | 2008-03-04 | Palantyr Research, Llc | Particle analyzing system and methodology |
US20030021016A1 (en) | 2001-07-27 | 2003-01-30 | Grier David G. | Parallel scanned laser confocal microscope |
US7186985B2 (en) | 2001-07-30 | 2007-03-06 | Dxray, Inc. | Method and apparatus for fabricating mercuric iodide polycrystalline films for digital radiography |
US6519033B1 (en) | 2001-11-19 | 2003-02-11 | Point Source Technologies, Llc | Identification of particles in fluid |
FR2830962B1 (fr) | 2001-10-12 | 2004-01-30 | Inst Nat Rech Inf Automat | Dispositif et methode de traitement d'image pour detection de lesions evolutives |
US6735960B2 (en) | 2001-10-30 | 2004-05-18 | Carnegie Institution Of Washington | Composition and method for hydrogen storage |
JP4358631B2 (ja) | 2001-12-04 | 2009-11-04 | エコール ポリテクニーク フェデラル ドゥ ローザンヌ(エーペーエフエル) | ディジタル・ホログラフィック・イメージングの装置及び方法 |
US6737634B2 (en) | 2002-01-16 | 2004-05-18 | The University Of Chicago | Use of multiple optical vortices for pumping, mixing and sorting |
AU2003216175A1 (en) | 2002-02-04 | 2003-09-02 | Colorado School Of Mines | Laminar flow-based separations of colloidal and cellular particles |
GB2385481B (en) | 2002-02-13 | 2004-01-07 | Fairfield Imaging Ltd | Microscopy imaging system and method |
US7161140B2 (en) | 2002-04-10 | 2007-01-09 | Arryx, Inc. | Apparatus and method to generate and control optical traps to manipulate small particles |
US20040180363A1 (en) | 2002-04-12 | 2004-09-16 | Dymeka Gossett Rooks Pitts Pllc | Configurable dynamic three dimensional array |
DE60324953D1 (de) | 2002-05-14 | 2009-01-08 | Arryx Inc | Optisch adressierter sensor mit breitem spektrum |
US7324282B2 (en) | 2002-05-14 | 2008-01-29 | Arryx, Inc. | Apparatus, system and method for applying optical gradient forces |
US6710874B2 (en) | 2002-07-05 | 2004-03-23 | Rashid Mavliev | Method and apparatus for detecting individual particles in a flowable sample |
US7699767B2 (en) | 2002-07-31 | 2010-04-20 | Arryx, Inc. | Multiple laminar flow-based particle and cellular separation with laser steering |
EP2806427A3 (en) | 2002-07-31 | 2015-04-22 | Premium Genetics (UK) Limited | System and method of sorting materials using holographic laser steering |
US6863406B2 (en) | 2002-08-01 | 2005-03-08 | The University Of Chicago | Apparatus and method for fabricating, sorting, and integrating materials with holographic optical traps |
US7515953B2 (en) | 2002-08-01 | 2009-04-07 | The Johns Hopkins University | Techniques for identifying molecular structures and treating cell types lining a body lumen using fluorescence |
MY134335A (en) | 2002-09-11 | 2007-12-31 | Jfe Eng Corp | Process for producing gas clathrate and production apparatus |
US7109473B2 (en) | 2002-09-16 | 2006-09-19 | University Of Chicago | Transverse optical accelerator and generalized optical vortices |
WO2004028682A2 (en) | 2002-09-27 | 2004-04-08 | Carlsberg A/S | Spatially encoded polymer matrix |
US20060003252A1 (en) | 2002-12-02 | 2006-01-05 | Taku Hirayama | Chemical amplification type silicone based positive photoresist composition |
US7351953B2 (en) | 2003-04-10 | 2008-04-01 | Arryx, Inc. | Apparatus and method to generate and control optical traps to manipulate small particles |
EP1620862B1 (en) | 2003-05-08 | 2015-07-08 | The University Court of the University of St. Andrews | Fractionation of particles |
WO2004104551A2 (en) | 2003-05-16 | 2004-12-02 | University Of Chicago | Optical fractionation methods and apparatus |
IL156856A (en) | 2003-07-09 | 2011-11-30 | Joseph Shamir | Method for particle size and concentration measurement |
WO2005027031A2 (en) | 2003-09-12 | 2005-03-24 | Cyvera Corporation | Method and apparatus for labeling using diffraction grating-based encoded optical identification elements |
CN101382619A (zh) | 2003-10-28 | 2009-03-11 | 阿而利克斯公司 | 使用全息光学俘获操纵和处理材料的系统和方法 |
GB2408587A (en) | 2003-11-28 | 2005-06-01 | Univ Hertfordshire | Optical particle manipulation systems |
WO2005062018A2 (en) * | 2003-12-22 | 2005-07-07 | Versamatrix A/S | Apparatus and methods for analysis and sorting of particles such as polymer beads |
US20070242269A1 (en) | 2004-03-06 | 2007-10-18 | Michael Trainer | Methods and apparatus for determining characteristics of particles |
WO2005100541A2 (en) | 2004-04-12 | 2005-10-27 | The Regents Of The University Of California | Optoelectronic tweezers for microparticle and cell manipulation |
JP2005337730A (ja) | 2004-05-24 | 2005-12-08 | Nara Institute Of Science & Technology | 測定システム |
US20060029634A1 (en) | 2004-08-06 | 2006-02-09 | Berg Michael C | Porous structures |
WO2006034129A2 (en) | 2004-09-17 | 2006-03-30 | Jmar Research, Inc. | Systems and methods for detecting scattered light from a particle using illumination incident at an angle |
WO2006058187A2 (en) | 2004-11-23 | 2006-06-01 | Robert Eric Betzig | Optical lattice microscopy |
US7473890B2 (en) | 2004-11-23 | 2009-01-06 | New York University | Manipulation of objects in potential energy landscapes |
WO2006063335A2 (en) | 2004-12-10 | 2006-06-15 | Arryx, Inc. | Automated extraction and purification of samples using optical tweezers |
US7586684B2 (en) | 2005-01-21 | 2009-09-08 | New York University | Solute characterization by optoelectronkinetic potentiometry in an inclined array of optical traps |
US7218112B2 (en) | 2005-05-12 | 2007-05-15 | Siemens Aktiengesellschaft | Combined MR/PET system |
WO2006130728A2 (en) | 2005-05-31 | 2006-12-07 | The Regents Of The University Of California | Single-cell raman spectroscopy for the non-destructive, non-invasive analysis of cells and cellular components |
US7230424B1 (en) | 2005-06-17 | 2007-06-12 | Fonar Corporation | Magnetic resonance imaging |
WO2007008921A2 (en) | 2005-07-12 | 2007-01-18 | Arryx, Inc. | Method and apparatus for forming multi-dimensional colloidal structures using holographic optical tweezers |
JP4581946B2 (ja) | 2005-09-29 | 2010-11-17 | セイコーエプソン株式会社 | 画像表示装置 |
US8149416B2 (en) | 2005-10-17 | 2012-04-03 | Arryx, Inc. | Apparatus and method for dynamic cellular probing and diagnostics using holographic optical forcing array |
US9056291B2 (en) | 2005-11-30 | 2015-06-16 | Micronics, Inc. | Microfluidic reactor system |
US7491928B2 (en) | 2005-12-05 | 2009-02-17 | New York University | Extended optical traps by shape-phase holography |
WO2007066450A1 (ja) | 2005-12-08 | 2007-06-14 | Nec Corporation | 楽曲における区画を抽出する区画自動抽出システム、区画自動抽出方法および区画自動抽出プログラム |
US20070177774A1 (en) | 2006-01-30 | 2007-08-02 | Nanogate Optoelectronics Robot, Inc. | Fingerprint Recognition System Having Detecting Pore and Method Thereof |
JP2007279475A (ja) | 2006-04-10 | 2007-10-25 | Pulstec Industrial Co Ltd | ホログラム記録装置及びホログラム記録方法 |
US7788067B2 (en) | 2006-05-12 | 2010-08-31 | Artium Technologies, Inc. | Means and methods for signal validation for sizing spherical objects |
EP1865430A3 (en) | 2006-06-05 | 2009-09-23 | Cambridge Research & Instrumentation, Inc. | Monte Carlo simulation using GPU units on personal computers |
US8626449B2 (en) | 2006-10-17 | 2014-01-07 | The Regents Of The University Of California | Biological cell sorting and characterization using aerosol mass spectrometry |
US7835051B2 (en) | 2006-10-17 | 2010-11-16 | New York University | Volumetric imaging of holographic optical traps |
WO2008063809A2 (en) | 2006-10-24 | 2008-05-29 | New York University | Three-dimensional holographic ring traps |
US7605919B2 (en) * | 2006-10-30 | 2009-10-20 | Brightwell Technologies Inc. | Method and apparatus for analyzing particles in a fluid |
WO2008127410A2 (en) | 2006-11-07 | 2008-10-23 | New York University | Holographic microfabrication and characterization system for soft matter and biological systems |
US20080150532A1 (en) | 2006-12-21 | 2008-06-26 | General Electric Company | Method and apparatus for measuring t1 relaxation |
US9105306B2 (en) | 2006-12-27 | 2015-08-11 | Nec Corporation | Identifying objects in images using object identity probabilities based on interframe distances |
US8331019B2 (en) | 2007-01-26 | 2012-12-11 | New York University | Holographic microscopy of holographically trapped three-dimensional nanorod structures |
JP5514553B2 (ja) | 2007-01-26 | 2014-06-04 | ニュー・ヨーク・ユニヴァーシティ | ホログラフィックにトラップされた3次元構造のホログラフィック顕微鏡法 |
EP2160352A2 (en) | 2007-05-24 | 2010-03-10 | Simone Arca | Method for the production of binary clathrate hydrates of hydrogen |
WO2008146616A1 (ja) | 2007-05-25 | 2008-12-04 | Nec Corporation | 画像音響区間群対応付け装置と方法およびプログラム |
JP2009002276A (ja) | 2007-06-22 | 2009-01-08 | Nippon Soken Inc | 粒子状物質の捕集量検出方法及び捕集量検出装置と排ガス浄化装置 |
JP4424396B2 (ja) | 2007-09-03 | 2010-03-03 | ソニー株式会社 | データ処理装置および方法、並びにデータ処理プログラムおよびデータ処理プログラムが記録された記録媒体 |
US7782459B2 (en) * | 2007-09-24 | 2010-08-24 | Process Metrix | Laser-based apparatus and method for measuring agglomerate concentration and mean agglomerate size |
US20100259263A1 (en) | 2007-11-14 | 2010-10-14 | Dominic Holland | Longitudinal registration of anatomy in magnetic resonance imaging |
EP2224291B1 (en) | 2007-11-22 | 2016-06-29 | National University Corporation Kyoto Institute of Technology | Digital holography device and phase shifting array |
EP2257057B1 (en) | 2008-03-19 | 2019-05-08 | National University Corporation Hokkaido University | Dynamic image search device and dynamic image search program |
US7908300B2 (en) | 2008-04-02 | 2011-03-15 | Honeywell International Inc. | Guided entry system for individuals for annotating process deviations |
AU2009256118A1 (en) | 2008-06-05 | 2009-12-10 | The Administrators Of The Tulane Educational Fund | Methods and instrumentation for during-synthesis monitoring of polymer functional evolution |
US7929132B2 (en) * | 2008-07-11 | 2011-04-19 | University Of Utah Research Foundation | Transmission microscopy using light emitted from nanoparticles |
GB0813907D0 (en) | 2008-07-30 | 2008-09-03 | Univ Durham | Sub-micron 3d holographic lithpgraphy |
US9618369B2 (en) | 2008-08-26 | 2017-04-11 | The University Court Of The University Of Glasgow | Uses of electromagnetic interference patterns |
DE102008044828B3 (de) | 2008-08-28 | 2010-04-15 | Siemens Aktiengesellschaft | Verwendung eines Magnetresonanz-Sequenzmodells zur formalen Beschreibung einer Messsequenz |
US20100055031A1 (en) | 2008-08-28 | 2010-03-04 | Dong June Ahn | Ice nanorods for hydrogen storage |
WO2010101671A1 (en) | 2009-01-16 | 2010-09-10 | New York University | Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy |
WO2011049937A1 (en) | 2009-10-19 | 2011-04-28 | Massachusetts Institute Of Technology | Kinetically efficient substrate for lipoic acid ligase |
US8763623B2 (en) | 2009-11-06 | 2014-07-01 | Massachusetts Institute Of Technology | Methods for handling solids in microfluidic systems |
CN102753954A (zh) | 2009-12-22 | 2012-10-24 | 纽约大学 | 利用光学力将胶粒分成多个通道:棱镜光学分离 |
US8405395B2 (en) | 2010-04-15 | 2013-03-26 | The General Hospital Corporation | Method for simultaneous multi-slice magnetic resonance imaging |
EP2378307A1 (en) | 2010-04-15 | 2011-10-19 | Commissariat à l'Énergie Atomique et aux Énergies Alternatives | Method and apparatus for compensating for B1 inhomogeneity in magnetic resonance imaging by nonselective three-dimensional tailored RF pulses |
JP2013533469A (ja) | 2010-05-25 | 2013-08-22 | アリックス インク | 免疫診断における生物学的および化学的分析および用途における粒子の位置的自由度の検出のための方法および装置 |
US9519129B2 (en) | 2010-11-05 | 2016-12-13 | New York University | Method and system for measuring porosity of particles |
KR101829947B1 (ko) | 2010-11-12 | 2018-02-19 | 유니베르시테 리브레 드 브룩크젤즈 | 투명 입자를 특성화 하기 위한 광학적 방법 |
US9767341B2 (en) * | 2010-12-14 | 2017-09-19 | The Regents Of The University Of California | Method and device for holographic opto-fluidic microscopy |
US8723518B2 (en) | 2011-03-18 | 2014-05-13 | Nicole SEIBERLICH | Nuclear magnetic resonance (NMR) fingerprinting |
US8653818B2 (en) | 2011-04-08 | 2014-02-18 | Siemens Aktiengesellschaft | Parallel transmission RF pulse design with local SAR constraints |
WO2012170352A2 (en) | 2011-06-06 | 2012-12-13 | The Regents Of The University Of California | Multiplex fluorescent particle detection using spatially distributed excitation |
US9371467B2 (en) | 2011-07-11 | 2016-06-21 | Toray Industries, Inc. | Forming material, paint material composition and production method for forming material |
US20140170735A1 (en) | 2011-09-25 | 2014-06-19 | Elizabeth A. Holmes | Systems and methods for multi-analysis |
US9684044B2 (en) | 2011-11-01 | 2017-06-20 | Hitachi, Ltd. | Magnetic resonance imaging apparatus and antenna device |
WO2013080164A1 (en) | 2011-12-02 | 2013-06-06 | Csir | Hologram processing method and system |
RU2605525C2 (ru) | 2012-02-09 | 2016-12-20 | Конинклейке Филипс Н.В. | Устройство обнаружения данных для использования в комбинации с устройством mri |
WO2013126554A1 (en) | 2012-02-24 | 2013-08-29 | New York University | Holographic microrefractometer |
US9625551B2 (en) | 2012-04-16 | 2017-04-18 | Ali Caglar Ozen | Magnetic resonance apparatus and data acquisition method with decoupling between transmit and receive coils |
AU2014216060A1 (en) * | 2013-02-18 | 2015-08-20 | Theranos Ip Company, Llc | Systems and methods for collecting and transmitting assay results |
US20170209864A1 (en) | 2013-03-15 | 2017-07-27 | Gpb Scientific, Llc | Methods and systems for processing particles |
EP3068298A4 (en) | 2013-11-15 | 2018-02-21 | New York University | Self calibrating parallel transmission by spin dynamic fingerprinting |
US9816948B2 (en) | 2014-04-18 | 2017-11-14 | University Of Georgia Research Foundation, Inc. | Computerized tomography detection of microbial damage of plant tissues |
US10261154B2 (en) | 2014-04-21 | 2019-04-16 | Case Western Reserve University | Nuclear magnetic resonance (NMR) fingerprinting tissue classification and image segmentation |
US9897675B2 (en) | 2014-05-28 | 2018-02-20 | Case Western Reserve University | Magnetic resonance fingerprinting (MRF) with simultaneous multivolume acquisition |
ES2913524T3 (es) * | 2014-11-12 | 2022-06-02 | Univ New York | Huellas coloidales para materiales blandos usando caracterización holográfica total |
WO2017040158A1 (en) * | 2015-08-28 | 2017-03-09 | Schlumberger Technology Corporation | Microrheology of fluids used at wellsite |
US10641696B2 (en) | 2015-09-18 | 2020-05-05 | New York University | Holographic detection and characterization of large impurity particles in precision slurries |
EP3414517B1 (en) * | 2016-02-08 | 2021-10-06 | New York University | Holographic characterization of protein aggregates |
KR20170141029A (ko) * | 2016-06-14 | 2017-12-22 | 조선대학교산학협력단 | 형태학적 셀파라미터 기반 적혈구 검사 방법 및 이에 사용되는 디지털 홀로그래픽 현미경 |
EP3575773A1 (en) | 2018-05-28 | 2019-12-04 | Universität für Bodenkultur Wien | A method for determining a three-dimensional particle distribution in a medium |
US11543338B2 (en) | 2019-10-25 | 2023-01-03 | New York University | Holographic characterization of irregular particles |
EP3922979B1 (en) | 2020-06-08 | 2023-06-07 | Universität für Bodenkultur Wien | Method for determining a focal length of a particle in a medium |
EP3922977A1 (en) | 2020-06-08 | 2021-12-15 | Universität für Bodenkultur Wien | Method for determining a property of a particle in a medium |
-
2017
- 2017-02-07 EP EP17750639.1A patent/EP3414517B1/en active Active
- 2017-02-07 WO PCT/US2017/016857 patent/WO2017139279A2/en active Application Filing
- 2017-02-07 US US16/076,265 patent/US11385157B2/en active Active
- 2017-02-07 ES ES17750639T patent/ES2901608T3/es active Active
- 2017-02-07 DK DK17750639.1T patent/DK3414517T3/da active
- 2017-02-07 JP JP2018541149A patent/JP7130242B2/ja active Active
- 2017-02-07 TW TW106103963A patent/TWI731030B/zh active
-
2022
- 2022-05-27 US US17/826,848 patent/US11747258B2/en active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2011502256A (ja) * | 2007-10-30 | 2011-01-20 | ニュー・ヨーク・ユニヴァーシティ | ホログラフィックビデオ顕微鏡による粒子の追跡および特徴付け |
WO2013019640A1 (en) * | 2011-07-29 | 2013-02-07 | The Regents Of The University Of California | Lensfree holographic microscopy using wetting films |
JP2014525583A (ja) * | 2011-08-29 | 2014-09-29 | アムジェン インコーポレイテッド | 流体中の非溶解粒子の非破壊的検出のための方法および装置 |
CN102411298A (zh) * | 2011-11-30 | 2012-04-11 | 昆明理工大学 | 一种三维实时超分辨数字全息记录方法 |
WO2015200512A1 (en) * | 2014-06-25 | 2015-12-30 | New York University | In-line particle characterization |
Non-Patent Citations (1)
Title |
---|
WANG, CHEN ET AL.: "Holographic Characterization of Protein Aggregates", JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 105, JPN6020050262, 2016, pages 1074 - 1085, XP009513938, ISSN: 0004420770, DOI: 10.1016/j.xphs.2015.12.018 * |
Also Published As
Publication number | Publication date |
---|---|
EP3414517A2 (en) | 2018-12-19 |
US20220326130A1 (en) | 2022-10-13 |
WO2017139279A2 (en) | 2017-08-17 |
US11747258B2 (en) | 2023-09-05 |
TWI731030B (zh) | 2021-06-21 |
JP7130242B2 (ja) | 2022-09-05 |
WO2017139279A3 (en) | 2017-10-26 |
ES2901608T3 (es) | 2022-03-23 |
DK3414517T3 (da) | 2021-12-13 |
EP3414517A4 (en) | 2020-02-19 |
TW201732258A (zh) | 2017-09-16 |
EP3414517B1 (en) | 2021-10-06 |
US11385157B2 (en) | 2022-07-12 |
US20210199551A1 (en) | 2021-07-01 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
US11747258B2 (en) | Holographic characterization of protein aggregates | |
Wang et al. | Holographic characterization of protein aggregates | |
US11892390B2 (en) | Automated real-time particle characterization and three-dimensional velocimetry with holographic video microscopy | |
EP2866099B1 (en) | Tracking and characterizing particles with holographic video microscopy | |
JP6030131B2 (ja) | 粒子の光学的な検出および解析 | |
Wang et al. | Holographic characterization of colloidal fractal aggregates | |
US20190234853A1 (en) | Holographic characterization using hu moments | |
JP2022001040A (ja) | 細胞の評価方法及び細胞解析装置 | |
Nikitin et al. | Study of laser beam scattering by inhomogeneous ensemble of red blood cells in a shear flow | |
Midtvedt et al. | Holographic characterisation of subwavelength particles enhanced by deep learning | |
Monakhova et al. | A Comparative Study of Different Software Packages for Nanoparticle Tracking Analysis | |
Cheong et al. | Flow visualization and flow cytometry with holographic video microscopy | |
Wang | Holographic Characterization of Mutable Colloidal Systems | |
Cannaday | Improved time-lapsed angular scattering microscopy of single cells |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20200205 |
|
A977 | Report on retrieval |
Free format text: JAPANESE INTERMEDIATE CODE: A971007 Effective date: 20201130 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20210107 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20210406 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20210604 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20210706 |
|
A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20211101 |
|
A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20220131 |
|
A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20220330 |
|
TRDD | Decision of grant or rejection written | ||
A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20220719 |
|
A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20220817 |
|
R150 | Certificate of patent or registration of utility model |
Ref document number: 7130242 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |