JP2018530336A5 - - Google Patents
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- JP2018530336A5 JP2018530336A5 JP2018518438A JP2018518438A JP2018530336A5 JP 2018530336 A5 JP2018530336 A5 JP 2018530336A5 JP 2018518438 A JP2018518438 A JP 2018518438A JP 2018518438 A JP2018518438 A JP 2018518438A JP 2018530336 A5 JP2018530336 A5 JP 2018530336A5
- Authority
- JP
- Japan
- Prior art keywords
- cell
- cells
- nucleic acid
- perv
- porcine
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021034493A JP7144122B2 (ja) | 2015-10-08 | 2021-03-04 | 多重ゲノム編集 |
| JP2021196238A JP7420439B2 (ja) | 2015-10-08 | 2021-12-02 | 多重ゲノム編集 |
| JP2022076779A JP7473136B2 (ja) | 2015-10-08 | 2022-05-06 | 多重ゲノム編集 |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562239239P | 2015-10-08 | 2015-10-08 | |
| US62/239,239 | 2015-10-08 | ||
| PCT/US2016/055916 WO2017062723A1 (en) | 2015-10-08 | 2016-10-07 | Multiplexed genome editing |
Related Child Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021034493A Division JP7144122B2 (ja) | 2015-10-08 | 2021-03-04 | 多重ゲノム編集 |
| JP2021196238A Division JP7420439B2 (ja) | 2015-10-08 | 2021-12-02 | 多重ゲノム編集 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018530336A JP2018530336A (ja) | 2018-10-18 |
| JP2018530336A5 true JP2018530336A5 (enExample) | 2019-12-12 |
| JP7059468B2 JP7059468B2 (ja) | 2022-04-26 |
Family
ID=58488520
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018518438A Active JP7059468B2 (ja) | 2015-10-08 | 2016-10-07 | 多重ゲノム編集 |
| JP2021034493A Active JP7144122B2 (ja) | 2015-10-08 | 2021-03-04 | 多重ゲノム編集 |
| JP2021196238A Active JP7420439B2 (ja) | 2015-10-08 | 2021-12-02 | 多重ゲノム編集 |
| JP2022076779A Active JP7473136B2 (ja) | 2015-10-08 | 2022-05-06 | 多重ゲノム編集 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021034493A Active JP7144122B2 (ja) | 2015-10-08 | 2021-03-04 | 多重ゲノム編集 |
| JP2021196238A Active JP7420439B2 (ja) | 2015-10-08 | 2021-12-02 | 多重ゲノム編集 |
| JP2022076779A Active JP7473136B2 (ja) | 2015-10-08 | 2022-05-06 | 多重ゲノム編集 |
Country Status (15)
| Country | Link |
|---|---|
| US (6) | US10959413B2 (enExample) |
| EP (2) | EP4491732A3 (enExample) |
| JP (4) | JP7059468B2 (enExample) |
| KR (2) | KR102880582B1 (enExample) |
| CN (2) | CN108513580A (enExample) |
| AU (2) | AU2016335698B2 (enExample) |
| CA (1) | CA3001314A1 (enExample) |
| CL (1) | CL2018000902A1 (enExample) |
| CO (1) | CO2018004763A2 (enExample) |
| IL (2) | IL297017A (enExample) |
| MX (2) | MX2018004263A (enExample) |
| NZ (1) | NZ741778A (enExample) |
| SA (2) | SA523441205B1 (enExample) |
| WO (1) | WO2017062723A1 (enExample) |
| ZA (1) | ZA201802461B (enExample) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| EP4282970A3 (en) * | 2012-12-17 | 2024-01-17 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| CA3176380A1 (en) | 2014-11-21 | 2016-05-26 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modification using paired guide rnas |
| IL297017A (en) * | 2015-10-08 | 2022-12-01 | Harvard College | Multiplexed genome editing |
| US12043852B2 (en) | 2015-10-23 | 2024-07-23 | President And Fellows Of Harvard College | Evolved Cas9 proteins for gene editing |
| EP3386550B1 (en) | 2015-12-07 | 2021-01-20 | Arc Bio, LLC | Methods for the making and using of guide nucleic acids |
| SG11201805132YA (en) | 2015-12-24 | 2018-07-30 | Selexis Sa | Improved eukaryotic cells for protein manufacturing and methods of making them |
| WO2017201476A1 (en) | 2016-05-20 | 2017-11-23 | Regeneron Pharmaceuticals, Inc. | Methods for breaking immunological tolerance using multiple guide rnas |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CN109804066A (zh) | 2016-08-09 | 2019-05-24 | 哈佛大学的校长及成员们 | 可编程cas9-重组酶融合蛋白及其用途 |
| US11542509B2 (en) | 2016-08-24 | 2023-01-03 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| AU2017342543B2 (en) | 2016-10-14 | 2024-06-27 | President And Fellows Of Harvard College | AAV delivery of nucleobase editors |
| EP3555285A4 (en) * | 2016-12-14 | 2020-07-08 | Dow AgroSciences LLC | RECONSTRUCTION OF SITE SPECIFIC NUCLEASE BINDING SITES |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| EP3592853A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Suppression of pain by gene editing |
| KR20190127797A (ko) | 2017-03-10 | 2019-11-13 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 시토신에서 구아닌으로의 염기 편집제 |
| CA3057192A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| US12058986B2 (en) * | 2017-04-20 | 2024-08-13 | Egenesis, Inc. | Method for generating a genetically modified pig with inactivated porcine endogenous retrovirus (PERV) elements |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| CN107254551B (zh) * | 2017-06-06 | 2020-04-07 | 中国人民解放军军事科学院军事医学研究院 | 鉴定和选育PERV-pol基因缺陷型五指山小型猪新品系的方法 |
| US20200190508A1 (en) * | 2017-06-07 | 2020-06-18 | Arc Bio, Llc | Creation and use of guide nucleic acids |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| WO2019139645A2 (en) | 2017-08-30 | 2019-07-18 | President And Fellows Of Harvard College | High efficiency base editors comprising gam |
| CA3082251A1 (en) | 2017-10-16 | 2019-04-25 | The Broad Institute, Inc. | Uses of adenosine base editors |
| EP3724214A4 (en) | 2017-12-15 | 2021-09-01 | The Broad Institute Inc. | SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| KR102158428B1 (ko) * | 2018-08-16 | 2020-09-22 | (주)라트바이오 | 인위적 뉴클레아제를 생산하는 형질전환 배아 및 형질전환 동물의 제작 방법 |
| US12281338B2 (en) | 2018-10-29 | 2025-04-22 | The Broad Institute, Inc. | Nucleobase editors comprising GeoCas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| DE112020001306T5 (de) | 2019-03-19 | 2022-01-27 | Massachusetts Institute Of Technology | Verfahren und zusammensetzungen zur editierung von nukleotidsequenzen |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| CN110358766A (zh) * | 2019-06-10 | 2019-10-22 | 云南农业大学 | 一种基于全基因组CRISPR/Cas9文库筛选异种移植抗原基因的方法 |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| CN111549060A (zh) * | 2020-05-07 | 2020-08-18 | 西南大学 | 一种真核生物CRISPR/Cas9全基因组编辑细胞文库及构建方法 |
| CN111534541A (zh) * | 2020-05-07 | 2020-08-14 | 西南大学 | 一种真核生物CRISPR-Cas9双gRNA载体及构建方法 |
| JP2023525304A (ja) | 2020-05-08 | 2023-06-15 | ザ ブロード インスティテュート，インコーポレーテッド | 標的二本鎖ヌクレオチド配列の両鎖同時編集のための方法および組成物 |
| CN112280802A (zh) * | 2020-05-21 | 2021-01-29 | 华中农业大学 | 基于piggyBac转座子系统制备稳定表达BE4蛋白的猪肾上皮细胞系的方法 |
| EP4426828A1 (en) | 2021-11-01 | 2024-09-11 | Tome Biosciences, Inc. | Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo |
| CA3244180A1 (en) | 2021-12-22 | 2023-06-29 | Tome Biosciences Inc | CO-ADMINISTRATION OF A GENE EDITOR CONSTRUCTION AND A PATTERN DONOR |
| CN114438083A (zh) * | 2022-01-29 | 2022-05-06 | 五邑大学 | 识别猪PERV基因的sgRNA及其编码DNA和应用 |
| WO2023205744A1 (en) | 2022-04-20 | 2023-10-26 | Tome Biosciences, Inc. | Programmable gene insertion compositions |
| WO2023225670A2 (en) | 2022-05-20 | 2023-11-23 | Tome Biosciences, Inc. | Ex vivo programmable gene insertion |
| WO2024020587A2 (en) | 2022-07-22 | 2024-01-25 | Tome Biosciences, Inc. | Pleiopluripotent stem cell programmable gene insertion |
| WO2024138194A1 (en) | 2022-12-22 | 2024-06-27 | Tome Biosciences, Inc. | Platforms, compositions, and methods for in vivo programmable gene insertion |
| WO2024234006A1 (en) | 2023-05-11 | 2024-11-14 | Tome Biosciences, Inc. | Systems, compositions, and methods for targeting liver sinusodial endothelial cells (lsecs) |
| WO2025050069A1 (en) | 2023-09-01 | 2025-03-06 | Tome Biosciences, Inc. | Programmable gene insertion using engineered integration enzymes |
| WO2025224107A1 (en) | 2024-04-22 | 2025-10-30 | Basecamp Research Ltd | Method and compositions for detecting off-target editing |
| WO2025224182A2 (en) | 2024-04-23 | 2025-10-30 | Basecamp Research Ltd | Single construct platform for simultaneous delivery of gene editing machinery and nucleic acid cargo |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1250594A4 (en) | 2000-01-19 | 2003-05-07 | Biotransplant Inc | PORC UNABLE TO TRANSMIT PORCINE ENDOGENEOUS RETROVIRUS, AND USES THEREOF |
| AU2004316293A1 (en) * | 2003-11-21 | 2005-09-09 | Revivicor, Inc. | Use of interfering RNA in the production of transgenic animals |
| EP1582591A3 (en) * | 2004-02-27 | 2005-12-21 | Bundesrepublik Deutschland vertreten durch das Bundesminsterium für Gesundheit, dieses vertr. durch das Robert-Koch-Institut | Sirna and their use for knock down expression of porcine endogenous retrovirus |
| NZ539491A (en) * | 2005-04-15 | 2008-04-30 | Living Cell Products Pty Ltd | Swine population and uses thereof |
| EP2077329A1 (en) * | 2008-01-04 | 2009-07-08 | MWM Biomodels GmbH | Transgenic pig with altered incretin function |
| EP2931899A1 (en) | 2012-12-12 | 2015-10-21 | The Broad Institute, Inc. | Functional genomics using crispr-cas systems, compositions, methods, knock out libraries and applications thereof |
| EP4282970A3 (en) * | 2012-12-17 | 2024-01-17 | President and Fellows of Harvard College | Rna-guided human genome engineering |
| JP7085716B2 (ja) | 2013-06-05 | 2022-06-17 | デューク ユニバーシティ | Ｒｎａガイド遺伝子編集及び遺伝子調節 |
| AU2014312123A1 (en) | 2013-08-29 | 2016-03-17 | Temple University Of The Commonwealth System Of Higher Education | Methods and compositions for RNA-guided treatment of HIV infection |
| EP3540051B1 (en) * | 2013-12-12 | 2022-08-17 | The Broad Institute, Inc. | Delivery, use and therapeutic applications of the crispr-cas systems and compositions for hsv and viral diseases and disorders. |
| MA41382A (fr) | 2015-03-20 | 2017-11-28 | Univ Temple | Édition génique basée sur le système crispr/endonucléase à induction par tat |
| DE102015207944A1 (de) | 2015-04-29 | 2016-11-03 | Wacker Chemie Ag | Verfahren zur Herstellung organisch modifizierter Aerogele |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| CN105154471A (zh) | 2015-08-11 | 2015-12-16 | 南方医科大学珠江医院 | 低表达或不表达perv的肝细胞的制备方法 |
| IL297017A (en) | 2015-10-08 | 2022-12-01 | Harvard College | Multiplexed genome editing |
| SG11201805132YA (en) | 2015-12-24 | 2018-07-30 | Selexis Sa | Improved eukaryotic cells for protein manufacturing and methods of making them |
| WO2018144546A1 (en) * | 2017-02-01 | 2018-08-09 | President And Fellows Of Harvard College | Methods for increasing efficiency of gene editing in cells |
| US12058986B2 (en) * | 2017-04-20 | 2024-08-13 | Egenesis, Inc. | Method for generating a genetically modified pig with inactivated porcine endogenous retrovirus (PERV) elements |
-
2016
- 2016-10-07 IL IL297017A patent/IL297017A/en unknown
- 2016-10-07 CN CN201680071844.2A patent/CN108513580A/zh active Pending
- 2016-10-07 US US15/766,551 patent/US10959413B2/en active Active
- 2016-10-07 JP JP2018518438A patent/JP7059468B2/ja active Active
- 2016-10-07 CA CA3001314A patent/CA3001314A1/en active Pending
- 2016-10-07 NZ NZ741778A patent/NZ741778A/en unknown
- 2016-10-07 AU AU2016335698A patent/AU2016335698B2/en active Active
- 2016-10-07 EP EP24214109.1A patent/EP4491732A3/en active Pending
- 2016-10-07 KR KR1020247037262A patent/KR102880582B1/ko active Active
- 2016-10-07 KR KR1020187012502A patent/KR102729669B1/ko active Active
- 2016-10-07 CN CN202211152246.1A patent/CN116064664A/zh active Pending
- 2016-10-07 MX MX2018004263A patent/MX2018004263A/es unknown
- 2016-10-07 WO PCT/US2016/055916 patent/WO2017062723A1/en not_active Ceased
- 2016-10-07 EP EP16854392.4A patent/EP3359661B1/en active Active
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2018
- 2018-02-05 US US15/888,581 patent/US10375938B2/en active Active
- 2018-04-06 MX MX2024007924A patent/MX2024007924A/es unknown
- 2018-04-06 CL CL2018000902A patent/CL2018000902A1/es unknown
- 2018-04-07 SA SA523441205A patent/SA523441205B1/ar unknown
- 2018-04-07 SA SA518391294A patent/SA518391294B1/ar unknown
- 2018-04-08 IL IL258536A patent/IL258536A/en unknown
- 2018-04-13 ZA ZA2018/02461A patent/ZA201802461B/en unknown
- 2018-05-03 CO CONC2018/0004763A patent/CO2018004763A2/es unknown
-
2019
- 2019-08-05 US US16/531,351 patent/US11064684B2/en active Active
- 2019-12-10 US US16/708,655 patent/US10925263B2/en active Active
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2020
- 2020-12-11 US US17/118,684 patent/US12133515B2/en active Active
-
2021
- 2021-03-04 JP JP2021034493A patent/JP7144122B2/ja active Active
- 2021-12-02 JP JP2021196238A patent/JP7420439B2/ja active Active
-
2022
- 2022-05-06 JP JP2022076779A patent/JP7473136B2/ja active Active
-
2023
- 2023-02-28 AU AU2023201178A patent/AU2023201178B2/en active Active
-
2024
- 2024-09-27 US US18/899,268 patent/US20250017181A1/en active Pending
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