JP2018524016A5 - - Google Patents
Download PDFInfo
- Publication number
- JP2018524016A5 JP2018524016A5 JP2018503555A JP2018503555A JP2018524016A5 JP 2018524016 A5 JP2018524016 A5 JP 2018524016A5 JP 2018503555 A JP2018503555 A JP 2018503555A JP 2018503555 A JP2018503555 A JP 2018503555A JP 2018524016 A5 JP2018524016 A5 JP 2018524016A5
- Authority
- JP
- Japan
- Prior art keywords
- period
- over
- minutes
- hours
- lysis solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000243 solution Substances 0.000 claims 17
- 230000009089 cytolysis Effects 0.000 claims 10
- 238000000034 method Methods 0.000 claims 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 6
- 150000007523 nucleic acids Chemical class 0.000 claims 6
- 102000039446 nucleic acids Human genes 0.000 claims 6
- 108020004707 nucleic acids Proteins 0.000 claims 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims 4
- 239000002518 antifoaming agent Substances 0.000 claims 4
- 239000002738 chelating agent Substances 0.000 claims 4
- 239000011780 sodium chloride Substances 0.000 claims 3
- 239000004094 surface-active agent Substances 0.000 claims 3
- VKZRWSNIWNFCIQ-UHFFFAOYSA-N 2-[2-(1,2-dicarboxyethylamino)ethylamino]butanedioic acid Chemical compound OC(=O)CC(C(O)=O)NCCNC(C(O)=O)CC(O)=O VKZRWSNIWNFCIQ-UHFFFAOYSA-N 0.000 claims 2
- QPCDCPDFJACHGM-UHFFFAOYSA-N N,N-bis{2-[bis(carboxymethyl)amino]ethyl}glycine Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(=O)O)CCN(CC(O)=O)CC(O)=O QPCDCPDFJACHGM-UHFFFAOYSA-N 0.000 claims 2
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims 2
- 239000000872 buffer Substances 0.000 claims 2
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 239000000839 emulsion Substances 0.000 claims 2
- 239000003960 organic solvent Substances 0.000 claims 2
- 229960003330 pentetic acid Drugs 0.000 claims 2
- FTEDXVNDVHYDQW-UHFFFAOYSA-N BAPTA Chemical compound OC(=O)CN(CC(O)=O)C1=CC=CC=C1OCCOC1=CC=CC=C1N(CC(O)=O)CC(O)=O FTEDXVNDVHYDQW-UHFFFAOYSA-N 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical group CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims 1
- 108091005804 Peptidases Proteins 0.000 claims 1
- 229920001213 Polysorbate 20 Polymers 0.000 claims 1
- 239000004365 Protease Substances 0.000 claims 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 claims 1
- 239000004288 Sodium dehydroacetate Substances 0.000 claims 1
- 230000003115 biocidal effect Effects 0.000 claims 1
- 239000003139 biocide Substances 0.000 claims 1
- 239000003124 biologic agent Substances 0.000 claims 1
- 238000001574 biopsy Methods 0.000 claims 1
- 239000003153 chemical reaction reagent Substances 0.000 claims 1
- KPUWHANPEXNPJT-UHFFFAOYSA-N disiloxane Chemical class [SiH3]O[SiH3] KPUWHANPEXNPJT-UHFFFAOYSA-N 0.000 claims 1
- CDMADVZSLOHIFP-UHFFFAOYSA-N disodium;3,7-dioxido-2,4,6,8,9-pentaoxa-1,3,5,7-tetraborabicyclo[3.3.1]nonane;decahydrate Chemical compound O.O.O.O.O.O.O.O.O.O.[Na+].[Na+].O1B([O-])OB2OB([O-])OB1O2 CDMADVZSLOHIFP-UHFFFAOYSA-N 0.000 claims 1
- 229960001484 edetic acid Drugs 0.000 claims 1
- 238000000605 extraction Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 239000002563 ionic surfactant Substances 0.000 claims 1
- 238000002493 microarray Methods 0.000 claims 1
- 239000002736 nonionic surfactant Substances 0.000 claims 1
- -1 o-aminophenoxy Chemical group 0.000 claims 1
- UEZVMMHDMIWARA-UHFFFAOYSA-M phosphonate Chemical compound [O-]P(=O)=O UEZVMMHDMIWARA-UHFFFAOYSA-M 0.000 claims 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims 1
- 239000003755 preservative agent Substances 0.000 claims 1
- 238000003757 reverse transcription PCR Methods 0.000 claims 1
- 238000007790 scraping Methods 0.000 claims 1
- RDZTWEVXRGYCFV-UHFFFAOYSA-M sodium 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonate Chemical compound [Na+].OCCN1CCN(CCS([O-])(=O)=O)CC1 RDZTWEVXRGYCFV-UHFFFAOYSA-M 0.000 claims 1
- 229940079839 sodium dehydroacetate Drugs 0.000 claims 1
- 235000019259 sodium dehydroacetate Nutrition 0.000 claims 1
- DSOWAKKSGYUMTF-GZOLSCHFSA-M sodium;(1e)-1-(6-methyl-2,4-dioxopyran-3-ylidene)ethanolate Chemical compound [Na+].C\C([O-])=C1/C(=O)OC(C)=CC1=O DSOWAKKSGYUMTF-GZOLSCHFSA-M 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562196774P | 2015-07-24 | 2015-07-24 | |
| US62/196,774 | 2015-07-24 | ||
| PCT/US2016/041917 WO2017019293A1 (en) | 2015-07-24 | 2016-07-12 | Compositions and methods for dna and rna extraction from tissue samples |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018524016A JP2018524016A (ja) | 2018-08-30 |
| JP2018524016A5 true JP2018524016A5 (cg-RX-API-DMAC7.html) | 2019-08-29 |
| JP7099951B2 JP7099951B2 (ja) | 2022-07-12 |
Family
ID=56550375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018503555A Active JP7099951B2 (ja) | 2015-07-24 | 2016-07-12 | 組織試料からのｄｎａ及びｒｎａ抽出のための組成物及び方法 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10465182B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3325647B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP7099951B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN108124451B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2016298048B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2993447C (cg-RX-API-DMAC7.html) |
| DK (1) | DK3325647T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2795048T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2017019293A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2886389A1 (en) | 2012-09-28 | 2014-04-03 | Cepheid | Methods for dna and rna extraction from fixed paraffin-embedded tissue samples |
| EP3325647B1 (en) | 2015-07-24 | 2020-02-19 | Cepheid | Compositions and methods for dna and rna extraction from tissue samples |
| US11807900B2 (en) * | 2018-06-21 | 2023-11-07 | Genomic Health, Inc. | Systems and methods for pre-analytical substrate processing |
| CN109234269B (zh) * | 2018-09-21 | 2021-10-12 | 深圳金普迈生物科技有限公司 | 从甲醛固定的微量样品中提取并扩增dna片段的方法 |
| KR102041333B1 (ko) * | 2018-12-24 | 2019-11-06 | 주식회사 덴오믹스 | 세포 용해 조성물 및 이를 이용한 핵산의 추출 방법 |
| CN110835628B (zh) * | 2019-11-25 | 2021-07-27 | 宁波艾捷康宁生物科技有限公司 | 一种石蜡切片基因组dna提取的除蜡裂解液及提取试剂盒和提取方法 |
| CN111057704B (zh) * | 2019-12-31 | 2022-07-05 | 广州市金圻睿生物科技有限责任公司 | 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法 |
| WO2022197351A1 (en) | 2021-03-16 | 2022-09-22 | Cepheid | Methods for discriminating bacterial and viral infections in a human subject |
| WO2023070123A1 (en) | 2021-10-22 | 2023-04-27 | Cepheid | Compositions and methods of diagnosing and treating tuberculosis |
| TWI771253B (zh) * | 2021-12-27 | 2022-07-11 | 財團法人工業技術研究院 | 戶外用的簡易式核酸萃取套組以及於戶外萃取樣本的核酸的方法 |
| CN117925598B (zh) * | 2023-12-12 | 2025-12-09 | 阔然生物医药科技(上海)有限公司 | 一种新鲜脑组织的核酸快速抽提方法 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958349A (en) | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
| AU758407B2 (en) | 1997-12-24 | 2003-03-20 | Cepheid | Integrated fluid manipulation cartridge |
| CA2270023C (en) | 1998-04-28 | 2010-10-12 | Ortho-Clinical Diagnostics, Inc. | Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6818185B1 (en) | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| JP4078073B2 (ja) | 1999-05-28 | 2008-04-23 | シーフィード | 流体試料分析装置および方法 |
| US6248535B1 (en) | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
| US6423488B1 (en) | 2000-01-15 | 2002-07-23 | Avigenics, Inc | High throughput screening assay for detecting a DNA sequence |
| US6403037B1 (en) | 2000-02-04 | 2002-06-11 | Cepheid | Reaction vessel and temperature control system |
| CN101268189A (zh) * | 2003-04-02 | 2008-09-17 | 亚钧阔特生命科学公司 | 分离核酸的方法 |
| WO2005007852A2 (en) | 2003-07-09 | 2005-01-27 | Genvault Corporation | Room temperature elution of nucleic acids |
| WO2006085104A1 (en) | 2005-02-11 | 2006-08-17 | Invitrogen Dynal As | Method for isolating nucleic acids comprising the use of ethylene glycol multimers |
| JP2007117084A (ja) * | 2005-09-28 | 2007-05-17 | Fujifilm Corp | 核酸抽出法 |
| CN101243186A (zh) * | 2005-09-28 | 2008-08-13 | 富士胶片株式会社 | 核酸的提取方法 |
| EP1783645A1 (en) | 2005-11-08 | 2007-05-09 | Actigenics | Methods for the identification of microRNA and their applications in research and human health |
| DE102005057334A1 (de) * | 2005-11-28 | 2007-06-06 | Aj Innuscreen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien |
| JP2007325562A (ja) * | 2006-06-09 | 2007-12-20 | Fujifilm Corp | 核酸抽出法 |
| JP2008043332A (ja) | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| US8080645B2 (en) | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| WO2008035991A2 (en) | 2006-09-19 | 2008-03-27 | Michael Ronald Cook | A nucleic acid extraction method |
| WO2009127350A1 (en) | 2008-04-15 | 2009-10-22 | Universität Bern | Method for optimized isolation of rna from fixed tissue |
| EP2193831A1 (de) | 2008-12-05 | 2010-06-09 | Qiagen GmbH | Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe |
| US20100240049A1 (en) | 2009-01-16 | 2010-09-23 | Cepheid | Methods of Detecting Cervical Cancer |
| EP2628803A3 (en) | 2009-02-25 | 2014-01-01 | Cepheid | Methods of detecting lung cancer |
| EP2478087B1 (en) | 2009-09-14 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| KR101590544B1 (ko) * | 2010-01-07 | 2016-02-18 | 빅텍 프라이빗 리미티드 | 핵산 분리법 및 그를 위한 키트 |
| US9422542B2 (en) | 2010-02-26 | 2016-08-23 | Qiagen Gmbh | Process for parallel isolation and/or purification of RNA and DNA |
| US20130023655A1 (en) * | 2010-04-08 | 2013-01-24 | Qiagen Gmbh | Method for precipitating anionic surfactant ions in the presence of nucleic acids |
| EP2395082A1 (en) | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
| JP2014526255A (ja) | 2011-09-13 | 2014-10-06 | キアゲン ゲーエムベーハー | 獣医学的全血試料から核酸を単離するための方法 |
| GB201209229D0 (en) | 2012-05-25 | 2012-07-04 | Epistem Ltd | Nucleic acid extraction |
| US9145553B2 (en) * | 2012-06-15 | 2015-09-29 | Ut-Battelle, Llc | Method for isolating nucleic acids |
| EP2708890B1 (en) | 2012-09-14 | 2015-11-18 | Molecular Devices (New Milton) Ltd | Method of selecting a monoclonal cell colony |
| CA2886389A1 (en) | 2012-09-28 | 2014-04-03 | Cepheid | Methods for dna and rna extraction from fixed paraffin-embedded tissue samples |
| CN105164258B (zh) | 2013-03-18 | 2021-05-18 | 凯杰有限公司 | 细胞外核酸的稳定化和分离 |
| EP2793026A1 (en) | 2013-04-18 | 2014-10-22 | Centre National de la Recherche Scientifique (CNRS) | New method for monitoring cancer and/or inflammatory reaction based on relb phosphorylation |
| CN104152438B (zh) * | 2014-08-15 | 2017-05-17 | 四川农业大学 | 用于提取家禽dna的细胞裂解液、试剂盒和方法 |
| WO2016205233A2 (en) * | 2015-06-15 | 2016-12-22 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| EP3325647B1 (en) | 2015-07-24 | 2020-02-19 | Cepheid | Compositions and methods for dna and rna extraction from tissue samples |
-
2016
- 2016-07-12 EP EP16742522.2A patent/EP3325647B1/en active Active
- 2016-07-12 US US15/208,525 patent/US10465182B2/en active Active
- 2016-07-12 EP EP20157569.3A patent/EP3705582B1/en active Active
- 2016-07-12 ES ES16742522T patent/ES2795048T3/es active Active
- 2016-07-12 DK DK16742522.2T patent/DK3325647T3/da active
- 2016-07-12 CN CN201680055819.5A patent/CN108124451B/zh active Active
- 2016-07-12 AU AU2016298048A patent/AU2016298048B2/en active Active
- 2016-07-12 JP JP2018503555A patent/JP7099951B2/ja active Active
- 2016-07-12 CA CA2993447A patent/CA2993447C/en active Active
- 2016-07-12 WO PCT/US2016/041917 patent/WO2017019293A1/en not_active Ceased
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2018524016A5 (cg-RX-API-DMAC7.html) | ||
| US20200225128A1 (en) | Simple fixation and stablisation | |
| Hooper et al. | Effects of severe heat stress on immune function, biochemistry and histopathology in farmed Australian abalone (hybrid Haliotis laevigata× Haliotis rubra) | |
| EP3705582B1 (en) | Compositions and methods for dna and rna extraction from tissue samples | |
| JP2013503639A5 (cg-RX-API-DMAC7.html) | ||
| Toledano et al. | Dual fluorescence detection of protein and RNA in Drosophila tissues | |
| US20130280728A1 (en) | Method and kit for processing wax-embedded biological samples | |
| US20230357825A1 (en) | Cell preservation reagents and methods of use | |
| US20170215410A1 (en) | Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue | |
| Papadaki et al. | Histological evaluation of sex differentiation and early sex identification in hatchery-produced greater amberjack (Seriola dumerili) reared in sea cages | |
| WO2023179216A1 (zh) | 一种生物样本提取液及其应用和使用方法 | |
| Walen | Normal human cell conversion to 3-D cancer-like growth: Genome damage, endopolyploidy, senecence escape, and cell polarity change/loss | |
| US20180355345A1 (en) | Method and composition | |
| Lyons et al. | Survival of tardigrades (Hypsibius exemplaris) to subzero temperatures depends on exposure intensity, duration, and ice-nucleation—as shown by large-scale mortality dye-based assays | |
| Nutton | Detection of male human DNA from whole Lucilia sericata (Meigen)(Diptera: Calliphoridae) larvae using the Quantifiler® Trio DNA Quantification Kit | |
| Golubeva et al. | Collection and preparation of rodent embryonic samples for transcriptome study | |
| Santhanagopalan et al. | Protocol to isolate nuclei from Chlamydomonas reinhardtii for ATAC sequencing | |
| CN105483119A (zh) | 一种固定线虫单条虫dna粗提液及其制备方法与应用 | |
| JP2019512274A5 (cg-RX-API-DMAC7.html) | ||
| HK1220481B (en) | Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue | |
| UA25661U (en) | Method for passivation of l929 cell culture |