ES2795048T3 - Composiciones y métodos para la extracción de ADN y ARN de muestras de tejidos - Google Patents
Composiciones y métodos para la extracción de ADN y ARN de muestras de tejidos Download PDFInfo
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- ES2795048T3 ES2795048T3 ES16742522T ES16742522T ES2795048T3 ES 2795048 T3 ES2795048 T3 ES 2795048T3 ES 16742522 T ES16742522 T ES 16742522T ES 16742522 T ES16742522 T ES 16742522T ES 2795048 T3 ES2795048 T3 ES 2795048T3
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562196774P | 2015-07-24 | 2015-07-24 | |
| PCT/US2016/041917 WO2017019293A1 (en) | 2015-07-24 | 2016-07-12 | Compositions and methods for dna and rna extraction from tissue samples |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2795048T3 true ES2795048T3 (es) | 2020-11-20 |
Family
ID=56550375
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES16742522T Active ES2795048T3 (es) | 2015-07-24 | 2016-07-12 | Composiciones y métodos para la extracción de ADN y ARN de muestras de tejidos |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10465182B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3325647B1 (cg-RX-API-DMAC7.html) |
| JP (1) | JP7099951B2 (cg-RX-API-DMAC7.html) |
| CN (1) | CN108124451B (cg-RX-API-DMAC7.html) |
| AU (1) | AU2016298048B2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2993447C (cg-RX-API-DMAC7.html) |
| DK (1) | DK3325647T3 (cg-RX-API-DMAC7.html) |
| ES (1) | ES2795048T3 (cg-RX-API-DMAC7.html) |
| WO (1) | WO2017019293A1 (cg-RX-API-DMAC7.html) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2886389A1 (en) | 2012-09-28 | 2014-04-03 | Cepheid | Methods for dna and rna extraction from fixed paraffin-embedded tissue samples |
| EP3325647B1 (en) | 2015-07-24 | 2020-02-19 | Cepheid | Compositions and methods for dna and rna extraction from tissue samples |
| US11807900B2 (en) * | 2018-06-21 | 2023-11-07 | Genomic Health, Inc. | Systems and methods for pre-analytical substrate processing |
| CN109234269B (zh) * | 2018-09-21 | 2021-10-12 | 深圳金普迈生物科技有限公司 | 从甲醛固定的微量样品中提取并扩增dna片段的方法 |
| KR102041333B1 (ko) * | 2018-12-24 | 2019-11-06 | 주식회사 덴오믹스 | 세포 용해 조성물 및 이를 이용한 핵산의 추출 방법 |
| CN110835628B (zh) * | 2019-11-25 | 2021-07-27 | 宁波艾捷康宁生物科技有限公司 | 一种石蜡切片基因组dna提取的除蜡裂解液及提取试剂盒和提取方法 |
| CN111057704B (zh) * | 2019-12-31 | 2022-07-05 | 广州市金圻睿生物科技有限责任公司 | 一种石蜡切片样本dna提取的脱蜡裂解结合液、试剂盒及方法 |
| WO2022197351A1 (en) | 2021-03-16 | 2022-09-22 | Cepheid | Methods for discriminating bacterial and viral infections in a human subject |
| WO2023070123A1 (en) | 2021-10-22 | 2023-04-27 | Cepheid | Compositions and methods of diagnosing and treating tuberculosis |
| TWI771253B (zh) * | 2021-12-27 | 2022-07-11 | 財團法人工業技術研究院 | 戶外用的簡易式核酸萃取套組以及於戶外萃取樣本的核酸的方法 |
| CN117925598B (zh) * | 2023-12-12 | 2025-12-09 | 阔然生物医药科技(上海)有限公司 | 一种新鲜脑组织的核酸快速抽提方法 |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5958349A (en) | 1997-02-28 | 1999-09-28 | Cepheid | Reaction vessel for heat-exchanging chemical processes |
| AU758407B2 (en) | 1997-12-24 | 2003-03-20 | Cepheid | Integrated fluid manipulation cartridge |
| CA2270023C (en) | 1998-04-28 | 2010-10-12 | Ortho-Clinical Diagnostics, Inc. | Improved methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6469159B1 (en) * | 1999-04-26 | 2002-10-22 | Robert T. Belly | Methods for extracting nucleic acids from tissue samples and paraffin-embedded tissues |
| US6818185B1 (en) | 1999-05-28 | 2004-11-16 | Cepheid | Cartridge for conducting a chemical reaction |
| JP4078073B2 (ja) | 1999-05-28 | 2008-04-23 | シーフィード | 流体試料分析装置および方法 |
| US6248535B1 (en) | 1999-12-20 | 2001-06-19 | University Of Southern California | Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens |
| US6423488B1 (en) | 2000-01-15 | 2002-07-23 | Avigenics, Inc | High throughput screening assay for detecting a DNA sequence |
| US6403037B1 (en) | 2000-02-04 | 2002-06-11 | Cepheid | Reaction vessel and temperature control system |
| CN101268189A (zh) * | 2003-04-02 | 2008-09-17 | 亚钧阔特生命科学公司 | 分离核酸的方法 |
| WO2005007852A2 (en) | 2003-07-09 | 2005-01-27 | Genvault Corporation | Room temperature elution of nucleic acids |
| WO2006085104A1 (en) | 2005-02-11 | 2006-08-17 | Invitrogen Dynal As | Method for isolating nucleic acids comprising the use of ethylene glycol multimers |
| JP2007117084A (ja) * | 2005-09-28 | 2007-05-17 | Fujifilm Corp | 核酸抽出法 |
| CN101243186A (zh) * | 2005-09-28 | 2008-08-13 | 富士胶片株式会社 | 核酸的提取方法 |
| EP1783645A1 (en) | 2005-11-08 | 2007-05-09 | Actigenics | Methods for the identification of microRNA and their applications in research and human health |
| DE102005057334A1 (de) * | 2005-11-28 | 2007-06-06 | Aj Innuscreen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien |
| JP2007325562A (ja) * | 2006-06-09 | 2007-12-20 | Fujifilm Corp | 核酸抽出法 |
| JP2008043332A (ja) | 2006-08-17 | 2008-02-28 | Panomics Inc | 組織スライドからの核酸定量 |
| US8080645B2 (en) | 2007-10-01 | 2011-12-20 | Longhorn Vaccines & Diagnostics Llc | Biological specimen collection/transport compositions and methods |
| WO2008035991A2 (en) | 2006-09-19 | 2008-03-27 | Michael Ronald Cook | A nucleic acid extraction method |
| WO2009127350A1 (en) | 2008-04-15 | 2009-10-22 | Universität Bern | Method for optimized isolation of rna from fixed tissue |
| EP2193831A1 (de) | 2008-12-05 | 2010-06-09 | Qiagen GmbH | Parallel-Extraktion von unterschiedlichen Biomolekülen aus Formalin-fixiertem Gewebe |
| US20100240049A1 (en) | 2009-01-16 | 2010-09-23 | Cepheid | Methods of Detecting Cervical Cancer |
| EP2628803A3 (en) | 2009-02-25 | 2014-01-01 | Cepheid | Methods of detecting lung cancer |
| EP2478087B1 (en) | 2009-09-14 | 2017-01-18 | QIAGEN Gaithersburg, Inc. | Compositions and methods for recovery of nucleic acids or proteins from tissue samples fixed in cytology media |
| KR101590544B1 (ko) * | 2010-01-07 | 2016-02-18 | 빅텍 프라이빗 리미티드 | 핵산 분리법 및 그를 위한 키트 |
| US9422542B2 (en) | 2010-02-26 | 2016-08-23 | Qiagen Gmbh | Process for parallel isolation and/or purification of RNA and DNA |
| US20130023655A1 (en) * | 2010-04-08 | 2013-01-24 | Qiagen Gmbh | Method for precipitating anionic surfactant ions in the presence of nucleic acids |
| EP2395082A1 (en) | 2010-06-14 | 2011-12-14 | QIAGEN GmbH | Extraction of nucleic acids from wax-embedded samples |
| JP2014526255A (ja) | 2011-09-13 | 2014-10-06 | キアゲン ゲーエムベーハー | 獣医学的全血試料から核酸を単離するための方法 |
| GB201209229D0 (en) | 2012-05-25 | 2012-07-04 | Epistem Ltd | Nucleic acid extraction |
| US9145553B2 (en) * | 2012-06-15 | 2015-09-29 | Ut-Battelle, Llc | Method for isolating nucleic acids |
| EP2708890B1 (en) | 2012-09-14 | 2015-11-18 | Molecular Devices (New Milton) Ltd | Method of selecting a monoclonal cell colony |
| CA2886389A1 (en) | 2012-09-28 | 2014-04-03 | Cepheid | Methods for dna and rna extraction from fixed paraffin-embedded tissue samples |
| CN105164258B (zh) | 2013-03-18 | 2021-05-18 | 凯杰有限公司 | 细胞外核酸的稳定化和分离 |
| EP2793026A1 (en) | 2013-04-18 | 2014-10-22 | Centre National de la Recherche Scientifique (CNRS) | New method for monitoring cancer and/or inflammatory reaction based on relb phosphorylation |
| CN104152438B (zh) * | 2014-08-15 | 2017-05-17 | 四川农业大学 | 用于提取家禽dna的细胞裂解液、试剂盒和方法 |
| WO2016205233A2 (en) * | 2015-06-15 | 2016-12-22 | Cepheid | Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge |
| EP3325647B1 (en) | 2015-07-24 | 2020-02-19 | Cepheid | Compositions and methods for dna and rna extraction from tissue samples |
-
2016
- 2016-07-12 EP EP16742522.2A patent/EP3325647B1/en active Active
- 2016-07-12 US US15/208,525 patent/US10465182B2/en active Active
- 2016-07-12 EP EP20157569.3A patent/EP3705582B1/en active Active
- 2016-07-12 ES ES16742522T patent/ES2795048T3/es active Active
- 2016-07-12 DK DK16742522.2T patent/DK3325647T3/da active
- 2016-07-12 CN CN201680055819.5A patent/CN108124451B/zh active Active
- 2016-07-12 AU AU2016298048A patent/AU2016298048B2/en active Active
- 2016-07-12 JP JP2018503555A patent/JP7099951B2/ja active Active
- 2016-07-12 CA CA2993447A patent/CA2993447C/en active Active
- 2016-07-12 WO PCT/US2016/041917 patent/WO2017019293A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| EP3325647B1 (en) | 2020-02-19 |
| CN108124451A (zh) | 2018-06-05 |
| US20170022493A1 (en) | 2017-01-26 |
| EP3705582B1 (en) | 2025-09-03 |
| AU2016298048A1 (en) | 2018-02-15 |
| EP3325647A1 (en) | 2018-05-30 |
| EP3705582A1 (en) | 2020-09-09 |
| US10465182B2 (en) | 2019-11-05 |
| AU2016298048B2 (en) | 2022-08-04 |
| CN108124451B (zh) | 2022-09-09 |
| CA2993447A1 (en) | 2017-02-02 |
| DK3325647T3 (da) | 2020-05-18 |
| JP7099951B2 (ja) | 2022-07-12 |
| JP2018524016A (ja) | 2018-08-30 |
| CA2993447C (en) | 2024-01-02 |
| WO2017019293A1 (en) | 2017-02-02 |
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