JP2018513681A5 - - Google Patents
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- JP2018513681A5 JP2018513681A5 JP2017552079A JP2017552079A JP2018513681A5 JP 2018513681 A5 JP2018513681 A5 JP 2018513681A5 JP 2017552079 A JP2017552079 A JP 2017552079A JP 2017552079 A JP2017552079 A JP 2017552079A JP 2018513681 A5 JP2018513681 A5 JP 2018513681A5
- Authority
- JP
- Japan
- Prior art keywords
- protein
- integrase
- sequence
- cas9
- recombinase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 108090000623 proteins and genes Proteins 0.000 claims 22
- 102000004169 proteins and genes Human genes 0.000 claims 22
- 108091033409 CRISPR Proteins 0.000 claims 12
- 102100034343 Integrase Human genes 0.000 claims 12
- 108010061833 Integrases Proteins 0.000 claims 12
- 108020001507 fusion proteins Proteins 0.000 claims 11
- 102000037865 fusion proteins Human genes 0.000 claims 11
- 108091033319 polynucleotide Proteins 0.000 claims 10
- 102000040430 polynucleotide Human genes 0.000 claims 10
- 239000002157 polynucleotide Substances 0.000 claims 10
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 7
- 102000018120 Recombinases Human genes 0.000 claims 7
- 108010091086 Recombinases Proteins 0.000 claims 7
- 150000007523 nucleic acids Chemical class 0.000 claims 7
- 108091026890 Coding region Proteins 0.000 claims 4
- 102000008579 Transposases Human genes 0.000 claims 4
- 108010020764 Transposases Proteins 0.000 claims 4
- 101710185494 Zinc finger protein Proteins 0.000 claims 4
- 102100023597 Zinc finger protein 816 Human genes 0.000 claims 4
- 230000003197 catalytic effect Effects 0.000 claims 3
- 108010051219 Cre recombinase Proteins 0.000 claims 2
- 102000052510 DNA-Binding Proteins Human genes 0.000 claims 2
- 101710096438 DNA-binding protein Proteins 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 2
- 239000002773 nucleotide Substances 0.000 claims 2
- 125000003729 nucleotide group Chemical group 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims 1
- -1 Cpf1 Proteins 0.000 claims 1
- 108020005004 Guide RNA Proteins 0.000 claims 1
- 108010002459 HIV Integrase Proteins 0.000 claims 1
- 108700020129 Human immunodeficiency virus 1 p31 integrase Proteins 0.000 claims 1
- 241000713666 Lentivirus Species 0.000 claims 1
- 241000713333 Mouse mammary tumor virus Species 0.000 claims 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 claims 1
- 238000010459 TALEN Methods 0.000 claims 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000004927 fusion Effects 0.000 claims 1
- 230000018883 protein targeting Effects 0.000 claims 1
- 230000008685 targeting Effects 0.000 claims 1
- 241000701161 unidentified adenovirus Species 0.000 claims 1
- 241001430294 unidentified retrovirus Species 0.000 claims 1
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021110120A JP2021176301A (ja) | 2015-03-31 | 2021-07-01 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2023121860A JP7599740B2 (ja) | 2015-03-31 | 2023-07-26 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2024205951A JP2025065094A (ja) | 2015-03-31 | 2024-11-27 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562140454P | 2015-03-31 | 2015-03-31 | |
| US62/140,454 | 2015-03-31 | ||
| US201562210451P | 2015-08-27 | 2015-08-27 | |
| US62/210,451 | 2015-08-27 | ||
| US201562240359P | 2015-10-12 | 2015-10-12 | |
| US62/240,359 | 2015-10-12 | ||
| PCT/US2016/025426 WO2016161207A1 (en) | 2015-03-31 | 2016-03-31 | Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021110120A Division JP2021176301A (ja) | 2015-03-31 | 2021-07-01 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2018513681A JP2018513681A (ja) | 2018-05-31 |
| JP2018513681A5 true JP2018513681A5 (enExample) | 2019-05-09 |
Family
ID=55745849
Family Applications (4)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017552079A Pending JP2018513681A (ja) | 2015-03-31 | 2016-03-31 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2021110120A Pending JP2021176301A (ja) | 2015-03-31 | 2021-07-01 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2023121860A Active JP7599740B2 (ja) | 2015-03-31 | 2023-07-26 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2024205951A Pending JP2025065094A (ja) | 2015-03-31 | 2024-11-27 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
Family Applications After (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021110120A Pending JP2021176301A (ja) | 2015-03-31 | 2021-07-01 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2023121860A Active JP7599740B2 (ja) | 2015-03-31 | 2023-07-26 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
| JP2024205951A Pending JP2025065094A (ja) | 2015-03-31 | 2024-11-27 | 細胞または生物のゲノムへのＤＮＡ配列の標的化組み込みのためのＣａｓ９レトロウイルスインテグラーゼおよびＣａｓ９レコンビナーゼ系 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20180080051A1 (enExample) |
| EP (2) | EP4600366A3 (enExample) |
| JP (4) | JP2018513681A (enExample) |
| KR (1) | KR102769515B1 (enExample) |
| CN (1) | CN108124453B (enExample) |
| DK (1) | DK3277805T3 (enExample) |
| ES (1) | ES3030433T3 (enExample) |
| WO (1) | WO2016161207A1 (enExample) |
Families Citing this family (75)
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| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
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| EP2994531B1 (en) | 2013-05-10 | 2018-03-28 | Sangamo Therapeutics, Inc. | Delivery methods and compositions for nuclease-mediated genome engineering |
| DK3004337T3 (da) * | 2013-05-29 | 2017-11-13 | Cellectis | Fremgangsmåde til konstruktion af T-celler til immunoterapi ved brug af RNA-guidet Cas nuklease-system |
| US11685935B2 (en) * | 2013-05-29 | 2023-06-27 | Cellectis | Compact scaffold of Cas9 in the type II CRISPR system |
| US9388430B2 (en) * | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| CN104404036B (zh) * | 2014-11-03 | 2017-12-01 | 赛业（苏州）生物科技有限公司 | 基于CRISPR/Cas9技术的条件性基因敲除方法 |
| EP4600366A3 (en) * | 2015-03-31 | 2025-10-22 | SOHM, Inc. | Cas 9 retroviral integrase systems for targeted incorporation of a dna sequence into a genome of a cell |
| US9790490B2 (en) * | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
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2016
- 2016-03-31 EP EP25170644.6A patent/EP4600366A3/en active Pending
- 2016-03-31 KR KR1020177031337A patent/KR102769515B1/ko active Active
- 2016-03-31 WO PCT/US2016/025426 patent/WO2016161207A1/en not_active Ceased
- 2016-03-31 EP EP16715977.1A patent/EP3277805B1/en active Active
- 2016-03-31 ES ES16715977T patent/ES3030433T3/es active Active
- 2016-03-31 DK DK16715977.1T patent/DK3277805T3/da active
- 2016-03-31 CN CN201680031466.5A patent/CN108124453B/zh active Active
- 2016-03-31 JP JP2017552079A patent/JP2018513681A/ja active Pending
- 2016-03-31 US US15/563,657 patent/US20180080051A1/en not_active Abandoned
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2021
- 2021-02-11 US US17/173,494 patent/US20220315952A1/en active Pending
- 2021-07-01 JP JP2021110120A patent/JP2021176301A/ja active Pending
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2023
- 2023-07-26 JP JP2023121860A patent/JP7599740B2/ja active Active
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2024
- 2024-11-27 JP JP2024205951A patent/JP2025065094A/ja active Pending
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