CN108124453B - 用于将DNA序列靶向并入细胞或生物体的基因组中的Cas9逆转录病毒整合酶和Cas9重组酶系统 - Google Patents
用于将DNA序列靶向并入细胞或生物体的基因组中的Cas9逆转录病毒整合酶和Cas9重组酶系统 Download PDFInfo
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- CN108124453B CN108124453B CN201680031466.5A CN201680031466A CN108124453B CN 108124453 B CN108124453 B CN 108124453B CN 201680031466 A CN201680031466 A CN 201680031466A CN 108124453 B CN108124453 B CN 108124453B
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- C12N15/90—Stable introduction of foreign DNA into chromosome
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- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
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- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
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- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
- C12N9/222—Clustered regularly interspaced short palindromic repeats [CRISPR]-associated [CAS] enzymes
- C12N9/226—Class 2 CAS enzyme complex, e.g. single CAS protein
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- C07K2319/00—Fusion polypeptide
- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
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- C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
- C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding
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- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562140454P | 2015-03-31 | 2015-03-31 | |
| US62/140,454 | 2015-03-31 | ||
| US201562210451P | 2015-08-27 | 2015-08-27 | |
| US62/210,451 | 2015-08-27 | ||
| US201562240359P | 2015-10-12 | 2015-10-12 | |
| US62/240,359 | 2015-10-12 | ||
| PCT/US2016/025426 WO2016161207A1 (en) | 2015-03-31 | 2016-03-31 | Cas 9 retroviral integrase and cas 9 recombinase systems for targeted incorporation of a dna sequence into a genome of a cell or organism |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108124453A CN108124453A (zh) | 2018-06-05 |
| CN108124453B true CN108124453B (zh) | 2022-04-05 |
Family
ID=55745849
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201680031466.5A Active CN108124453B (zh) | 2015-03-31 | 2016-03-31 | 用于将DNA序列靶向并入细胞或生物体的基因组中的Cas9逆转录病毒整合酶和Cas9重组酶系统 |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20180080051A1 (enExample) |
| EP (2) | EP4600366A3 (enExample) |
| JP (4) | JP2018513681A (enExample) |
| KR (1) | KR102769515B1 (enExample) |
| CN (1) | CN108124453B (enExample) |
| DK (1) | DK3277805T3 (enExample) |
| ES (1) | ES3030433T3 (enExample) |
| WO (1) | WO2016161207A1 (enExample) |
Families Citing this family (75)
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| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| US9737604B2 (en) | 2013-09-06 | 2017-08-22 | President And Fellows Of Harvard College | Use of cationic lipids to deliver CAS9 |
| US11053481B2 (en) | 2013-12-12 | 2021-07-06 | President And Fellows Of Harvard College | Fusions of Cas9 domains and nucleic acid-editing domains |
| AU2015298571B2 (en) | 2014-07-30 | 2020-09-03 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| EP3204496A1 (en) | 2014-10-10 | 2017-08-16 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| AU2015342749B2 (en) | 2014-11-07 | 2022-01-27 | Editas Medicine, Inc. | Methods for improving CRISPR/Cas-mediated genome-editing |
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| KR20230123492A (ko) * | 2020-12-16 | 2023-08-23 | 우니베르시타트 폼페우 파브라 | 프로그래밍 가능한 트랜스포사제 및 이의 용도 |
| WO2024227131A1 (en) * | 2023-04-27 | 2024-10-31 | Rensselaer Polytechnic Institute | Recombinant enzyme for the accurate insertion of dna sequences in eukaryotic cells |
| CN118497204B (zh) * | 2024-07-16 | 2024-12-03 | 西北农林科技大学深圳研究院 | 一种质粒CRISPR-pCas9n及基因编辑的方法与应用 |
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| WO2016161207A1 (en) | 2016-10-06 |
| JP7599740B2 (ja) | 2024-12-16 |
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| US20220315952A1 (en) | 2022-10-06 |
| JP2018513681A (ja) | 2018-05-31 |
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| DK3277805T3 (da) | 2025-06-10 |
| US20180080051A1 (en) | 2018-03-22 |
| JP2021176301A (ja) | 2021-11-11 |
| EP3277805A1 (en) | 2018-02-07 |
| CN108124453A (zh) | 2018-06-05 |
| JP2023156355A (ja) | 2023-10-24 |
| JP2025065094A (ja) | 2025-04-17 |
| EP4600366A3 (en) | 2025-10-22 |
| EP3277805B1 (en) | 2025-05-07 |
| ES3030433T3 (en) | 2025-06-30 |
| KR20180029953A (ko) | 2018-03-21 |
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