JP2018510867A5 - - Google Patents
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- Publication number
- JP2018510867A5 JP2018510867A5 JP2017548109A JP2017548109A JP2018510867A5 JP 2018510867 A5 JP2018510867 A5 JP 2018510867A5 JP 2017548109 A JP2017548109 A JP 2017548109A JP 2017548109 A JP2017548109 A JP 2017548109A JP 2018510867 A5 JP2018510867 A5 JP 2018510867A5
- Authority
- JP
- Japan
- Prior art keywords
- wash solution
- chromatography
- protein
- matrix
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 16
- 239000012504 chromatography matrix Substances 0.000 claims 6
- 102000004169 proteins and genes Human genes 0.000 claims 6
- 108090000623 proteins and genes Proteins 0.000 claims 6
- 239000000203 mixture Substances 0.000 claims 5
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims 4
- 239000004475 Arginine Substances 0.000 claims 3
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 claims 3
- 238000001042 affinity chromatography Methods 0.000 claims 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims 3
- 150000001483 arginine derivatives Chemical class 0.000 claims 3
- 210000004748 cultured cell Anatomy 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 238000004587 chromatography analysis Methods 0.000 claims 2
- 210000004962 mammalian cell Anatomy 0.000 claims 2
- 229910000029 sodium carbonate Inorganic materials 0.000 claims 2
- 239000006228 supernatant Substances 0.000 claims 2
- 241000699802 Cricetulus griseus Species 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 238000005277 cation exchange chromatography Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 claims 1
- 239000013592 cell lysate Substances 0.000 claims 1
- 230000001143 conditioned effect Effects 0.000 claims 1
- 239000000356 contaminant Substances 0.000 claims 1
- 238000010828 elution Methods 0.000 claims 1
- 239000012530 fluid Substances 0.000 claims 1
- 238000004191 hydrophobic interaction chromatography Methods 0.000 claims 1
- 238000004255 ion exchange chromatography Methods 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 238000012434 mixed-mode chromatography Methods 0.000 claims 1
- 210000001672 ovary Anatomy 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201562132974P | 2015-03-13 | 2015-03-13 | |
| US62/132,974 | 2015-03-13 | ||
| PCT/US2016/021984 WO2016149088A1 (en) | 2015-03-13 | 2016-03-11 | Use of alkaline washes during chromatography to remove impurities |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2018510867A JP2018510867A (ja) | 2018-04-19 |
| JP2018510867A5 true JP2018510867A5 (OSRAM) | 2019-03-22 |
| JP6807859B2 JP6807859B2 (ja) | 2021-01-06 |
Family
ID=55699796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017548109A Active JP6807859B2 (ja) | 2015-03-13 | 2016-03-11 | クロマトグラフィーにおける不純物を取り除くためのアルカリ洗浄の使用 |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US10695693B2 (OSRAM) |
| EP (2) | EP4194071A1 (OSRAM) |
| JP (1) | JP6807859B2 (OSRAM) |
| KR (1) | KR102490956B1 (OSRAM) |
| CN (1) | CN107636005B (OSRAM) |
| AU (2) | AU2016233557B2 (OSRAM) |
| BR (1) | BR112017018365A2 (OSRAM) |
| CA (1) | CA2978095C (OSRAM) |
| EA (1) | EA035635B1 (OSRAM) |
| IL (1) | IL254372B (OSRAM) |
| MX (1) | MX395730B (OSRAM) |
| SG (2) | SG11201707330RA (OSRAM) |
| WO (1) | WO2016149088A1 (OSRAM) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016073401A1 (en) * | 2014-11-03 | 2016-05-12 | Bristol-Myers Squibb Company | Use of caprylic acid precipitation for protein purification |
| MX395730B (es) | 2015-03-13 | 2025-03-25 | Bristol Myers Squibb Co | Uso de lavados alcalinos durante la cromatografia para remover impurezas. |
| SG11201905510XA (en) | 2017-01-30 | 2019-08-27 | Regeneron Pharma | Compositions and methods for reducing bioburden in chromatography |
| CN112566930A (zh) * | 2018-06-19 | 2021-03-26 | 百时美施贵宝公司 | 使用色谱纯化蛋白质的方法 |
| JP7527273B2 (ja) * | 2018-08-17 | 2024-08-02 | リジェネロン・ファーマシューティカルズ・インコーポレイテッド | 多量体タンパク質の量および純度を決定するための方法およびクロマトグラフィーシステム |
| KR102270048B1 (ko) * | 2020-07-10 | 2021-06-28 | 주식회사 녹십자 | 바리셀라 조스터 바이러스 표면 단백질 항원의 생산 방법 |
| KR20210111243A (ko) * | 2018-10-31 | 2021-09-10 | 리제너론 파아마슈티컬스, 인크. | 단백질을 식별 및 정량화하는 방법 및 시스템 |
| WO2020125757A1 (en) * | 2018-12-21 | 2020-06-25 | Wuxi Biologics (Shanghai) Co., Ltd. | A method for improving aggregate removal by protein a chromatography |
| AU2020208360B2 (en) * | 2019-01-16 | 2025-05-08 | Regeneron Pharmaceuticals, Inc. | Method and system of identifying and quantifying antibody fragmentation |
| CN109870428A (zh) * | 2019-01-21 | 2019-06-11 | 江苏华泰疫苗工程技术研究有限公司 | 一种基于表面等离子共振技术的抗体定量检测方法 |
| GB201904741D0 (en) * | 2019-04-04 | 2019-05-22 | Glaxosmithkline Ip Dev Ltd | Novel process |
| CN114667450B (zh) * | 2019-09-24 | 2025-05-02 | 里珍纳龙药品有限公司 | 用于色谱介质的使用和再生的系统及方法 |
| KR20220079844A (ko) * | 2019-10-14 | 2022-06-14 | 피어스 바이오테크놀로지, 인크 | 펩티드 정제 제제 및 방법 |
| WO2021191684A1 (en) * | 2020-03-27 | 2021-09-30 | Haemalogix Pty Ltd | Composition and method |
| WO2025178923A1 (en) * | 2024-02-20 | 2025-08-28 | Exelixis, Inc. | Methods for protein purification |
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| US5225539A (en) | 1986-03-27 | 1993-07-06 | Medical Research Council | Recombinant altered antibodies and methods of making altered antibodies |
| US5190859A (en) * | 1987-02-26 | 1993-03-02 | Dana-Farber Cancer Institute, Inc. | Purification of LFA-3 |
| JPS63276494A (ja) * | 1987-02-26 | 1988-11-14 | ダナーファーバー・キャンサー・インスチチュート・インコーポレーテッド | Ｌｆａ−３の精製法 |
| CA1323567C (en) * | 1987-10-05 | 1993-10-26 | Gene R. Nathans | Method for purification of antibodies |
| US5476996A (en) | 1988-06-14 | 1995-12-19 | Lidak Pharmaceuticals | Human immune system in non-human animal |
| US5223409A (en) | 1988-09-02 | 1993-06-29 | Protein Engineering Corp. | Directed evolution of novel binding proteins |
| GB8823869D0 (en) | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| US5530101A (en) | 1988-12-28 | 1996-06-25 | Protein Design Labs, Inc. | Humanized immunoglobulins |
| ATE139258T1 (de) | 1990-01-12 | 1996-06-15 | Cell Genesys Inc | Erzeugung xenogener antikörper |
| US6150584A (en) | 1990-01-12 | 2000-11-21 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US6673986B1 (en) | 1990-01-12 | 2004-01-06 | Abgenix, Inc. | Generation of xenogeneic antibodies |
| US6075181A (en) | 1990-01-12 | 2000-06-13 | Abgenix, Inc. | Human antibodies derived from immunized xenomice |
| US5427908A (en) | 1990-05-01 | 1995-06-27 | Affymax Technologies N.V. | Recombinant library screening methods |
| US6172197B1 (en) | 1991-07-10 | 2001-01-09 | Medical Research Council | Methods for producing members of specific binding pairs |
| GB9015198D0 (en) * | 1990-07-10 | 1990-08-29 | Brien Caroline J O | Binding substance |
| US5633425A (en) | 1990-08-29 | 1997-05-27 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5814318A (en) | 1990-08-29 | 1998-09-29 | Genpharm International Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US5874299A (en) | 1990-08-29 | 1999-02-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| AU8507191A (en) | 1990-08-29 | 1992-03-30 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5661016A (en) | 1990-08-29 | 1997-08-26 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US6300129B1 (en) | 1990-08-29 | 2001-10-09 | Genpharm International | Transgenic non-human animals for producing heterologous antibodies |
| US5545806A (en) | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5625126A (en) | 1990-08-29 | 1997-04-29 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| US6255458B1 (en) | 1990-08-29 | 2001-07-03 | Genpharm International | High affinity human antibodies and human antibodies against digoxin |
| US5770429A (en) | 1990-08-29 | 1998-06-23 | Genpharm International, Inc. | Transgenic non-human animals capable of producing heterologous antibodies |
| US5877397A (en) | 1990-08-29 | 1999-03-02 | Genpharm International Inc. | Transgenic non-human animals capable of producing heterologous antibodies of various isotypes |
| US5789650A (en) | 1990-08-29 | 1998-08-04 | Genpharm International, Inc. | Transgenic non-human animals for producing heterologous antibodies |
| ES2341666T3 (es) | 1991-12-02 | 2010-06-24 | Medimmune Limited | Produccion de autoanticuerpos de repertorios de segmentos de anticue rpos expresados en la superficie de fagos. |
| EP0746609A4 (en) | 1991-12-17 | 1997-12-17 | Genpharm Int | NON-HUMAN TRANSGENIC ANIMALS CAPABLE OF PRODUCING HETEROLOGOUS ANTIBODIES |
| DE69303494T2 (de) | 1992-11-13 | 1997-01-16 | Idec Pharma Corp | Therapeutische verwendung von chimerischen und markierten antikörper gegen menschlichen b lymphozyt beschränkter differenzierung antigen für die behandlung von b-zell-lymphoma |
| JPH08509612A (ja) | 1993-04-26 | 1996-10-15 | ジェンファーム インターナショナル インコーポレイテッド | 異種抗体を産生することができるトランスジェニック非ヒト動物 |
| US5534615A (en) | 1994-04-25 | 1996-07-09 | Genentech, Inc. | Cardiac hypertrophy factor and uses therefor |
| KR20010043323A (ko) * | 1998-05-06 | 2001-05-25 | 제넨테크, 인크. | 이온 교환 크로마토그래피에 의한 단백질 정제 방법 |
| CN1371416B (zh) | 1999-08-24 | 2012-10-10 | 梅达里克斯公司 | 人ctla-4抗体及其应用 |
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| KR101374149B1 (ko) * | 2005-04-26 | 2014-03-14 | 베링거 인겔하임 에르체파우 게엠베하 운트 코 카게 | 융합 단백질의 자가단백질분해성 절단에 의한 재조합단백질 생산 |
| WO2007109163A2 (en) * | 2006-03-16 | 2007-09-27 | Amgen Inc | Wash buffer and method of using |
| CA2661748C (en) * | 2006-08-28 | 2016-02-09 | Alex Eon-Duval | Process for the purification of fc-containing proteins |
| WO2008031020A2 (en) | 2006-09-08 | 2008-03-13 | Wyeth | Arginine wash in protein purification using affinity chromatography |
| PE20091434A1 (es) * | 2007-10-30 | 2009-10-17 | Genentech Inc | Purificacion de anticuerpos por cromatografia de intercambio cationico |
| NZ592095A (en) * | 2008-10-20 | 2013-01-25 | Abbott Lab | Isolation and purification of il-12 and tnf-alpha antibodies using protein a affinity chromatography |
| CA2784278C (en) | 2009-12-18 | 2019-02-26 | Novartis Ag | Wash solution and method for affinity chromatography |
| JP2012001462A (ja) | 2010-06-15 | 2012-01-05 | Kaneka Corp | 抗体精製用カラム |
| EP2714714B1 (en) | 2011-06-01 | 2018-06-20 | Novartis AG | Wash solution and method for affinity chromatography |
| AR096713A1 (es) * | 2013-06-25 | 2016-01-27 | Cadila Healthcare Ltd | Proceso de purificación para anticuerpos monoclonales |
| TWI563003B (en) * | 2013-12-26 | 2016-12-21 | Mitsubishi Tanabe Pharma Corp | Human anti-il-33 neutralization monoclonal antibody |
| WO2016031932A1 (ja) | 2014-08-27 | 2016-03-03 | 田辺三菱製薬株式会社 | アルカリ洗浄によるＦｃ領域を有するタンパク質の製造方法 |
| MX395730B (es) | 2015-03-13 | 2025-03-25 | Bristol Myers Squibb Co | Uso de lavados alcalinos durante la cromatografia para remover impurezas. |
-
2016
- 2016-03-11 MX MX2017011121A patent/MX395730B/es unknown
- 2016-03-11 EP EP22206303.4A patent/EP4194071A1/en active Pending
- 2016-03-11 US US15/558,033 patent/US10695693B2/en active Active
- 2016-03-11 KR KR1020177028511A patent/KR102490956B1/ko active Active
- 2016-03-11 EA EA201792012A patent/EA035635B1/ru not_active IP Right Cessation
- 2016-03-11 SG SG11201707330RA patent/SG11201707330RA/en unknown
- 2016-03-11 WO PCT/US2016/021984 patent/WO2016149088A1/en not_active Ceased
- 2016-03-11 CN CN201680015033.0A patent/CN107636005B/zh active Active
- 2016-03-11 CA CA2978095A patent/CA2978095C/en active Active
- 2016-03-11 EP EP16715383.2A patent/EP3268100A1/en not_active Withdrawn
- 2016-03-11 BR BR112017018365A patent/BR112017018365A2/pt not_active Application Discontinuation
- 2016-03-11 SG SG10201908027Q patent/SG10201908027QA/en unknown
- 2016-03-11 JP JP2017548109A patent/JP6807859B2/ja active Active
- 2016-03-11 AU AU2016233557A patent/AU2016233557B2/en active Active
-
2017
- 2017-09-07 IL IL254372A patent/IL254372B/en unknown
-
2020
- 2020-05-22 US US16/881,102 patent/US12157073B2/en active Active
-
2021
- 2021-08-26 AU AU2021221870A patent/AU2021221870A1/en not_active Abandoned
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