JP2016529489A5 - - Google Patents
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- Publication number
- JP2016529489A5 JP2016529489A5 JP2016525805A JP2016525805A JP2016529489A5 JP 2016529489 A5 JP2016529489 A5 JP 2016529489A5 JP 2016525805 A JP2016525805 A JP 2016525805A JP 2016525805 A JP2016525805 A JP 2016525805A JP 2016529489 A5 JP2016529489 A5 JP 2016529489A5
- Authority
- JP
- Japan
- Prior art keywords
- polypeptide
- buffer
- contaminants
- gradient
- chromatography material
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000034 method Methods 0.000 claims 59
- 229920001184 polypeptide Polymers 0.000 claims 59
- 102000004196 processed proteins & peptides Human genes 0.000 claims 59
- 108090000765 processed proteins & peptides Proteins 0.000 claims 59
- 239000000463 material Substances 0.000 claims 26
- 239000000203 mixture Substances 0.000 claims 26
- 239000000872 buffer Substances 0.000 claims 23
- 239000000356 contaminant Substances 0.000 claims 23
- 238000004587 chromatography analysis Methods 0.000 claims 16
- 238000004255 ion exchange chromatography Methods 0.000 claims 13
- 150000003839 salts Chemical class 0.000 claims 11
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical group [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 8
- 239000012149 elution buffer Substances 0.000 claims 8
- 239000012160 loading buffer Substances 0.000 claims 8
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims 4
- 238000000926 separation method Methods 0.000 claims 4
- 239000011780 sodium chloride Substances 0.000 claims 4
- 239000007995 HEPES buffer Substances 0.000 claims 3
- 239000002253 acid Substances 0.000 claims 3
- 150000001413 amino acids Chemical class 0.000 claims 3
- 238000005277 cation exchange chromatography Methods 0.000 claims 3
- 238000010494 dissociation reaction Methods 0.000 claims 3
- 230000005593 dissociations Effects 0.000 claims 3
- 238000005342 ion exchange Methods 0.000 claims 3
- DBXNUXBLKRLWFA-UHFFFAOYSA-N N-(2-acetamido)-2-aminoethanesulfonic acid Chemical group NC(=O)CNCCS(O)(=O)=O DBXNUXBLKRLWFA-UHFFFAOYSA-N 0.000 claims 2
- 150000001412 amines Chemical group 0.000 claims 2
- 239000012634 fragment Substances 0.000 claims 2
- 230000007935 neutral effect Effects 0.000 claims 2
- 150000003512 tertiary amines Chemical class 0.000 claims 2
- -1 (NH 4 ) 2 SO 4 Substances 0.000 claims 1
- 239000007991 ACES buffer Substances 0.000 claims 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 claims 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 claims 1
- 238000005349 anion exchange Methods 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 230000003139 buffering effect Effects 0.000 claims 1
- 238000005341 cation exchange Methods 0.000 claims 1
- 238000013375 chromatographic separation Methods 0.000 claims 1
- 239000007857 degradation product Substances 0.000 claims 1
- 238000012434 mixed-mode chromatography Methods 0.000 claims 1
- 230000035772 mutation Effects 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361845890P | 2013-07-12 | 2013-07-12 | |
| US61/845,890 | 2013-07-12 | ||
| PCT/US2014/046338 WO2015006686A1 (en) | 2013-07-12 | 2014-07-11 | Elucidation of ion exchange chromatography input optimization |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018242206A Division JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2016529489A JP2016529489A (ja) | 2016-09-23 |
| JP2016529489A5 true JP2016529489A5 (OSRAM) | 2017-08-31 |
| JP6462681B2 JP6462681B2 (ja) | 2019-01-30 |
Family
ID=51257626
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016525805A Active JP6462681B2 (ja) | 2013-07-12 | 2014-07-11 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
| JP2018242206A Pending JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2018242206A Pending JP2019090818A (ja) | 2013-07-12 | 2018-12-26 | イオン交換クロマトグラフィーのインプットの最適化の解明 |
Country Status (18)
| Country | Link |
|---|---|
| US (2) | US10274466B2 (OSRAM) |
| EP (2) | EP3019516B1 (OSRAM) |
| JP (2) | JP6462681B2 (OSRAM) |
| KR (1) | KR102251127B1 (OSRAM) |
| CN (1) | CN105377873B (OSRAM) |
| AU (1) | AU2014287044B2 (OSRAM) |
| BR (1) | BR112016000231A8 (OSRAM) |
| CA (1) | CA2918052A1 (OSRAM) |
| ES (1) | ES2705700T3 (OSRAM) |
| HR (1) | HRP20190071T1 (OSRAM) |
| IL (1) | IL243305B (OSRAM) |
| MX (1) | MX368107B (OSRAM) |
| PL (1) | PL3019516T3 (OSRAM) |
| RU (1) | RU2016104542A (OSRAM) |
| SG (1) | SG11201600221YA (OSRAM) |
| SI (1) | SI3019516T1 (OSRAM) |
| TR (1) | TR201818966T4 (OSRAM) |
| WO (1) | WO2015006686A1 (OSRAM) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3019516B1 (en) | 2013-07-12 | 2018-11-14 | H. Hoffnabb-La Roche Ag | Elucidation of ion exchange chromatography input optimization |
| US11460455B2 (en) | 2014-11-12 | 2022-10-04 | Cytiva Sweden Ab | Method and system for determining the influence of experimental parameters on a liquid chromatography protocol |
| GB2539420B (en) * | 2015-06-16 | 2021-01-13 | Cytiva Sweden Ab | Determination of chromatography conditions |
| US11407838B2 (en) * | 2018-04-02 | 2022-08-09 | Amgen Inc. | Erenumab compositions and uses thereof |
| WO2020010304A2 (en) * | 2018-07-05 | 2020-01-09 | Ovation Diagnostics Llc | BCl-2 Antibodies and Immunoassay for Diagnosis of Cancer |
| KR20200080748A (ko) * | 2018-12-27 | 2020-07-07 | 주식회사 폴루스 | 음이온 교환 크로마토그래피를 이용한 인슐린 전구체의 정제방법 |
| WO2024261724A1 (en) * | 2023-06-23 | 2024-12-26 | Lupin Limited | Ion-exchange chromatography for separation and analysis of charge variants |
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| EP3019516B1 (en) | 2013-07-12 | 2018-11-14 | H. Hoffnabb-La Roche Ag | Elucidation of ion exchange chromatography input optimization |
-
2014
- 2014-07-11 EP EP14745049.8A patent/EP3019516B1/en active Active
- 2014-07-11 JP JP2016525805A patent/JP6462681B2/ja active Active
- 2014-07-11 SG SG11201600221YA patent/SG11201600221YA/en unknown
- 2014-07-11 ES ES14745049T patent/ES2705700T3/es active Active
- 2014-07-11 MX MX2016000181A patent/MX368107B/es active IP Right Grant
- 2014-07-11 CA CA2918052A patent/CA2918052A1/en not_active Abandoned
- 2014-07-11 PL PL14745049T patent/PL3019516T3/pl unknown
- 2014-07-11 AU AU2014287044A patent/AU2014287044B2/en active Active
- 2014-07-11 KR KR1020167003178A patent/KR102251127B1/ko active Active
- 2014-07-11 HR HRP20190071TT patent/HRP20190071T1/hr unknown
- 2014-07-11 WO PCT/US2014/046338 patent/WO2015006686A1/en not_active Ceased
- 2014-07-11 TR TR2018/18966T patent/TR201818966T4/tr unknown
- 2014-07-11 RU RU2016104542A patent/RU2016104542A/ru not_active Application Discontinuation
- 2014-07-11 BR BR112016000231A patent/BR112016000231A8/pt active Search and Examination
- 2014-07-11 SI SI201431039T patent/SI3019516T1/sl unknown
- 2014-07-11 CN CN201480039460.3A patent/CN105377873B/zh active Active
- 2014-07-11 EP EP18204792.8A patent/EP3536699A1/en not_active Withdrawn
- 2014-07-11 US US14/904,422 patent/US10274466B2/en active Active
-
2015
- 2015-12-23 IL IL243305A patent/IL243305B/en active IP Right Grant
-
2018
- 2018-12-26 JP JP2018242206A patent/JP2019090818A/ja active Pending
-
2019
- 2019-03-12 US US16/351,372 patent/US10921297B2/en active Active
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