JP2017538428A5 - - Google Patents
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- JP2017538428A5 JP2017538428A5 JP2017532808A JP2017532808A JP2017538428A5 JP 2017538428 A5 JP2017538428 A5 JP 2017538428A5 JP 2017532808 A JP2017532808 A JP 2017532808A JP 2017532808 A JP2017532808 A JP 2017532808A JP 2017538428 A5 JP2017538428 A5 JP 2017538428A5
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- Japan
- Prior art keywords
- nucleic acid
- acid insert
- ltvec
- sequence
- cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 150000007523 nucleic acids Chemical class 0.000 claims description 153
- 108020004707 nucleic acids Proteins 0.000 claims description 149
- 102000039446 nucleic acids Human genes 0.000 claims description 149
- 238000000034 method Methods 0.000 claims description 91
- 210000004027 cell Anatomy 0.000 claims description 50
- 230000008685 targeting Effects 0.000 claims description 20
- 101710163270 Nuclease Proteins 0.000 claims description 12
- 238000012217 deletion Methods 0.000 claims description 11
- 230000037430 deletion Effects 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 10
- 108020004414 DNA Proteins 0.000 claims description 8
- 238000010348 incorporation Methods 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 241000894007 species Species 0.000 claims description 7
- 239000012634 fragment Substances 0.000 claims description 6
- 210000001161 mammalian embryo Anatomy 0.000 claims description 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 5
- 230000005782 double-strand break Effects 0.000 claims description 5
- 210000004962 mammalian cell Anatomy 0.000 claims description 5
- 230000005783 single-strand break Effects 0.000 claims description 5
- 241000283984 Rodentia Species 0.000 claims description 4
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims description 4
- 210000005260 human cell Anatomy 0.000 claims description 4
- 238000003780 insertion Methods 0.000 claims description 4
- 230000037431 insertion Effects 0.000 claims description 4
- 230000010354 integration Effects 0.000 claims description 4
- 230000035772 mutation Effects 0.000 claims description 4
- 102000004169 proteins and genes Human genes 0.000 claims description 4
- 230000001105 regulatory effect Effects 0.000 claims description 4
- 108091033409 CRISPR Proteins 0.000 claims description 3
- 230000006798 recombination Effects 0.000 claims description 3
- 238000005215 recombination Methods 0.000 claims description 3
- 239000012636 effector Substances 0.000 claims description 2
- 210000002950 fibroblast Anatomy 0.000 claims description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 2
- 230000002103 transcriptional effect Effects 0.000 claims description 2
- 108700028369 Alleles Proteins 0.000 claims 2
- 108700008625 Reporter Genes Proteins 0.000 claims 2
- 238000000338 in vitro Methods 0.000 claims 2
- 239000003550 marker Substances 0.000 claims 2
- 108091033319 polynucleotide Proteins 0.000 claims 2
- 102000040430 polynucleotide Human genes 0.000 claims 2
- 239000002157 polynucleotide Substances 0.000 claims 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 1
- 238000010354 CRISPR gene editing Methods 0.000 claims 1
- 230000002068 genetic effect Effects 0.000 claims 1
- 210000001178 neural stem cell Anatomy 0.000 claims 1
- 230000006801 homologous recombination Effects 0.000 description 4
- 238000002744 homologous recombination Methods 0.000 description 4
- 108020005004 Guide RNA Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462094104P | 2014-12-19 | 2014-12-19 | |
| US62/094,104 | 2014-12-19 | ||
| US201562167408P | 2015-05-28 | 2015-05-28 | |
| US62/167,408 | 2015-05-28 | ||
| US201562205524P | 2015-08-14 | 2015-08-14 | |
| US62/205,524 | 2015-08-14 | ||
| PCT/US2015/066681 WO2016100819A1 (en) | 2014-12-19 | 2015-12-18 | Methods and compositions for targeted genetic modification through single-step multiple targeting |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020219029A Division JP7095066B2 (ja) | 2014-12-19 | 2020-12-28 | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017538428A JP2017538428A (ja) | 2017-12-28 |
| JP2017538428A5 true JP2017538428A5 (cg-RX-API-DMAC7.html) | 2019-01-24 |
| JP6840077B2 JP6840077B2 (ja) | 2021-03-10 |
Family
ID=55229831
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017532808A Active JP6840077B2 (ja) | 2014-12-19 | 2015-12-18 | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
| JP2020219029A Active JP7095066B2 (ja) | 2014-12-19 | 2020-12-28 | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020219029A Active JP7095066B2 (ja) | 2014-12-19 | 2020-12-28 | 単一ステップの複数標的化を通じた標的化された遺伝子修飾のための方法及び組成物 |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US11326184B2 (cg-RX-API-DMAC7.html) |
| EP (2) | EP3653048B9 (cg-RX-API-DMAC7.html) |
| JP (2) | JP6840077B2 (cg-RX-API-DMAC7.html) |
| KR (1) | KR102530821B1 (cg-RX-API-DMAC7.html) |
| CN (1) | CN107208113A (cg-RX-API-DMAC7.html) |
| AU (1) | AU2015364427B2 (cg-RX-API-DMAC7.html) |
| BR (1) | BR112017013104A2 (cg-RX-API-DMAC7.html) |
| CA (1) | CA2971213C (cg-RX-API-DMAC7.html) |
| ES (2) | ES2760508T3 (cg-RX-API-DMAC7.html) |
| IL (1) | IL252755B (cg-RX-API-DMAC7.html) |
| MX (1) | MX388784B (cg-RX-API-DMAC7.html) |
| NZ (1) | NZ732895A (cg-RX-API-DMAC7.html) |
| RU (1) | RU2707137C2 (cg-RX-API-DMAC7.html) |
| SG (1) | SG11201704646YA (cg-RX-API-DMAC7.html) |
| WO (1) | WO2016100819A1 (cg-RX-API-DMAC7.html) |
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| EP3461885B1 (en) | 2014-06-26 | 2021-10-20 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modifications and methods of use |
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| EP4559939A3 (en) | 2020-12-23 | 2025-09-24 | Regeneron Pharmaceuticals, Inc. | Nucleic acids encoding anchor modified antibodies and uses therof |
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| WO2015163733A1 (en) | 2014-04-24 | 2015-10-29 | Institute For Basic Science | A method of selecting a nuclease target sequence for gene knockout based on microhomology |
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| LT3152312T (lt) | 2014-06-06 | 2020-04-27 | Regeneron Pharmaceuticals, Inc. | Tikslinio lokuso modifikavimo būdai ir kompozicijos |
| EP3461885B1 (en) | 2014-06-26 | 2021-10-20 | Regeneron Pharmaceuticals, Inc. | Methods and compositions for targeted genetic modifications and methods of use |
| JP2017529841A (ja) * | 2014-09-19 | 2017-10-12 | リジェネロン・ファーマシューティカルズ・インコーポレイテッドＲｅｇｅｎｅｒｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | キメラ抗原受容体 |
| WO2016061073A1 (en) | 2014-10-14 | 2016-04-21 | Memorial Sloan-Kettering Cancer Center | Composition and method for in vivo engineering of chromosomal rearrangements |
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| WO2016089866A1 (en) | 2014-12-01 | 2016-06-09 | President And Fellows Of Harvard College | Rna-guided systems for in vivo gene editing |
| NZ732895A (en) | 2014-12-19 | 2022-05-27 | Regeneron Pharma | Methods and compositions for targeted genetic modification through single-step multiple targeting |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
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2015
- 2015-12-18 NZ NZ732895A patent/NZ732895A/en unknown
- 2015-12-18 RU RU2017124909A patent/RU2707137C2/ru active
- 2015-12-18 EP EP19201384.5A patent/EP3653048B9/en active Active
- 2015-12-18 AU AU2015364427A patent/AU2015364427B2/en active Active
- 2015-12-18 MX MX2017008190A patent/MX388784B/es unknown
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- 2015-12-18 EP EP15828604.7A patent/EP3232774B1/en active Active
- 2015-12-18 KR KR1020177019826A patent/KR102530821B1/ko active Active
- 2015-12-18 CN CN201580069507.5A patent/CN107208113A/zh active Pending
- 2015-12-18 US US14/974,623 patent/US11326184B2/en active Active
- 2015-12-18 ES ES15828604T patent/ES2760508T3/es active Active
- 2015-12-18 BR BR112017013104A patent/BR112017013104A2/pt not_active Application Discontinuation
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- 2015-12-18 ES ES19201384T patent/ES2947714T3/es active Active
- 2015-12-18 SG SG11201704646YA patent/SG11201704646YA/en unknown
- 2015-12-18 WO PCT/US2015/066681 patent/WO2016100819A1/en not_active Ceased
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2017
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2020
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2022
- 2022-04-05 US US17/713,696 patent/US20220235381A1/en active Pending
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