JP2017537645A - 細胞の選択された部分集団中の複数エピトープを識別するための方法 - Google Patents

細胞の選択された部分集団中の複数エピトープを識別するための方法 Download PDF

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JP2017537645A
JP2017537645A JP2017532096A JP2017532096A JP2017537645A JP 2017537645 A JP2017537645 A JP 2017537645A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017532096 A JP2017532096 A JP 2017532096A JP 2017537645 A JP2017537645 A JP 2017537645A
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ゲイリー ピー. ノーラン,
ゲイリー ピー. ノーラン,
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アプライズ バイオ, インコーポレイテッド
アプライズ バイオ, インコーポレイテッド
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JP2017532096A 2014-12-19 2015-12-21 細胞の選択された部分集団中の複数エピトープを識別するための方法 Pending JP2017537645A (ja)

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US201462094919P 2014-12-19 2014-12-19
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US62/094,924 2014-12-19
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PCT/US2015/067147 WO2016100976A2 (en) 2014-12-19 2015-12-21 Methods for identifying multiple epitopes in selected sub-populations of cells

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Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
JP6069224B2 (ja) 2011-01-31 2017-02-01 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
US11560585B2 (en) 2011-01-31 2023-01-24 Roche Sequencing Solutions, Inc. Methods of identifying multiple epitopes in cells
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3216609C (en) 2012-08-14 2024-05-14 10X Genomics, Inc. Microcapsule compositions and methods
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014093676A1 (en) 2012-12-14 2014-06-19 10X Technologies, Inc. Methods and systems for processing polynucleotides
AU2014214682B2 (en) 2013-02-08 2018-07-26 10X Genomics, Inc. Polynucleotide barcode generation
ES2711168T3 (es) 2013-08-28 2019-04-30 Becton Dickinson Co Análisis masivo en paralelo de células individuales
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
CN113249435B (zh) 2014-06-26 2024-09-03 10X基因组学有限公司 分析来自单个细胞或细胞群体的核酸的方法
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
MX2016016904A (es) 2014-06-26 2017-03-27 10X Genomics Inc Analisis de secuencias de acidos nucleicos.
US10900065B2 (en) 2014-11-14 2021-01-26 University Of Washington Methods and kits for labeling cellular molecules
SG11201705615UA (en) 2015-01-12 2017-08-30 10X Genomics Inc Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
EP4286516A3 (en) 2015-02-24 2024-03-06 10X Genomics, Inc. Partition processing methods and systems
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
CN107580632B (zh) 2015-04-23 2021-12-28 贝克顿迪金森公司 用于全转录组扩增的方法和组合物
CN108026524A (zh) 2015-09-11 2018-05-11 赛卢拉研究公司 用于核酸文库标准化的方法和组合物
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
EP4717780A2 (en) 2016-07-22 2026-04-01 Verily Life Sciences LLC Quantitative massively parallel proteomics
CA3034924C (en) 2016-09-26 2025-11-18 Becton, Dickinson And Company MEASUREMENT OF PROTEIN EXPRESSION USING REAGENTS WITH BARCODED OLIGONUCLEOTIDE SEQUENCES
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
GB201622222D0 (en) * 2016-12-23 2017-02-08 Cs Genetics Ltd Reagents and methods for molecular barcoding of nucleic acids of single cells
JP7033602B2 (ja) * 2017-01-27 2022-03-10 エフ.ホフマン-ラ ロシュ アーゲー ロングレンジ配列決定のためのバーコードを付けられたdna
CN110214186B (zh) 2017-01-30 2023-11-24 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10844372B2 (en) 2017-05-26 2020-11-24 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN109526228B (zh) 2017-05-26 2022-11-25 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
EP3447152A1 (en) * 2017-08-25 2019-02-27 Eawag, Swiss Federal Institute of Aquatic Science and Technology Accurate and massively parallel quantification of nucleic acid
CN111386351A (zh) 2017-09-22 2020-07-07 华盛顿大学 细胞分子的原位组合标记
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
EP3568494B1 (en) * 2017-12-08 2021-05-05 10X Genomics, Inc. Methods and compositions for labeling cells
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
US11841371B2 (en) 2018-03-13 2023-12-12 The Broad Institute, Inc. Proteomics and spatial patterning using antenna networks
CN112262218B (zh) 2018-04-06 2024-11-08 10X基因组学有限公司 用于单细胞处理中的质量控制的系统和方法
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
US11039856B2 (en) 2018-05-10 2021-06-22 Verily Life Sciences Llc Surgical laparoscopic ports for implanting medical systems
US11414701B2 (en) 2018-05-24 2022-08-16 The Broad Institute, Inc. Multimodal readouts for quantifying and sequencing nucleic acids in single cells
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
JP7618548B2 (ja) 2018-11-08 2025-01-21 ベクトン・ディキンソン・アンド・カンパニー ランダムプライミングを使用した単一細胞の全トランスクリプトーム解析
CA3120713A1 (en) * 2018-11-21 2020-05-28 Avida Biomed, Inc. Methods for targeted nucleic acid library formation
WO2020117914A1 (en) * 2018-12-04 2020-06-11 Roche Sequencing Solutions, Inc. Spatially oriented quantum barcoding of cellular targets
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
SG10201904897UA (en) * 2019-05-30 2020-12-30 Nat Univ Singapore Method, structures and system for nucleic acid sequence topology assembly for multiplexed profiling of proteins.
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
WO2021034791A1 (en) * 2019-08-19 2021-02-25 Inscripta, Inc. Optimizing high-throughput sequencing capacity
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長v(d)j情報を得るためのランダムプライミングの使用
US12163189B2 (en) 2019-11-27 2024-12-10 University Of Washington Method for preparation and high-throughput microbial single-cell RNA sequencing of bacteria
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ, インク. 核酸結合免疫サンドイッチアッセイ(nulisa)
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
EP4090764A1 (en) 2020-01-13 2022-11-23 Becton, Dickinson and Company Cell capture using du-containing oligonucleotides
US12595462B2 (en) * 2020-01-14 2026-04-07 William Marsh Rice University High throughput genetic barcoding and analysis methods
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
EP4111168A1 (en) 2020-02-25 2023-01-04 Becton Dickinson and Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
EP4150118A1 (en) 2020-05-14 2023-03-22 Becton Dickinson and Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
WO2021249816A1 (en) * 2020-06-08 2021-12-16 F. Hoffmann-La Roche Ag Method of single-cell analysis of multiple samples
US20230340578A1 (en) * 2020-07-08 2023-10-26 Roche Sequencing Solutions, Inc. Spatial analysis of multiple targets in tissue samples
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
US11739443B2 (en) 2020-11-20 2023-08-29 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
EP4263859A1 (en) 2020-12-15 2023-10-25 Becton, Dickinson and Company Single cell secretome analysis
CN120158497A (zh) 2021-02-23 2025-06-17 10X基因组学有限公司 基于探针的核酸和蛋白质分析
US20250129405A1 (en) * 2021-09-08 2025-04-24 Becton, Dickinson And Company Non-sequencing pcr-based method for detection of antibody-conjugated oligonucleotides

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524419A (ja) * 2000-02-18 2003-08-19 ウルフ・ランデグレン 近接プロービング方法およびキット
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
US20100203543A1 (en) * 2007-08-10 2010-08-12 Peter Oliver Krutzik Biological encoding of large numbers of cells
US20100240101A1 (en) * 2009-03-19 2010-09-23 Massachusetts Institute Of Technology Parallel Proximity Ligation Event Analysis
JP2012502625A (ja) * 2008-09-16 2012-02-02 ナチュラル エンヴァイロメント リサーチ カウンシル 樹状細胞調節分子
WO2014026032A2 (en) * 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
JP2014507141A (ja) * 2011-02-09 2014-03-27 ナテラ, インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
JP2014513916A (ja) * 2011-01-31 2014-06-19 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
WO2014099744A1 (en) * 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5242794A (en) 1984-12-13 1993-09-07 Applied Biosystems, Inc. Detection of specific sequences in nucleic acids
US4988617A (en) 1988-03-25 1991-01-29 California Institute Of Technology Method of detecting a nucleotide change in nucleic acids
US5494810A (en) 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
SE9400088D0 (sv) 1994-01-14 1994-01-14 Kabi Pharmacia Ab Bacterial receptor structures
US6582938B1 (en) 2001-05-11 2003-06-24 Affymetrix, Inc. Amplification of nucleic acids
US6625546B2 (en) * 2000-02-03 2003-09-23 Nanoscale Combinatorial Synthesis, Inc. Structure identification methods using mass measurements
US7473767B2 (en) 2001-07-03 2009-01-06 The Institute For Systems Biology Methods for detection and quantification of analytes in complex mixtures
US8853185B2 (en) * 2008-04-09 2014-10-07 Cornell University Coferons and methods of making and using them
US9315857B2 (en) * 2009-12-15 2016-04-19 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse label-tags

Patent Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2003524419A (ja) * 2000-02-18 2003-08-19 ウルフ・ランデグレン 近接プロービング方法およびキット
WO2005123963A2 (en) * 2004-06-14 2005-12-29 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for use in analyte detection using proximity probes
US20100203543A1 (en) * 2007-08-10 2010-08-12 Peter Oliver Krutzik Biological encoding of large numbers of cells
JP2012502625A (ja) * 2008-09-16 2012-02-02 ナチュラル エンヴァイロメント リサーチ カウンシル 樹状細胞調節分子
US20100240101A1 (en) * 2009-03-19 2010-09-23 Massachusetts Institute Of Technology Parallel Proximity Ligation Event Analysis
JP2014513916A (ja) * 2011-01-31 2014-06-19 アプライズ バイオ, インコーポレイテッド 細胞において複数のエピトープを同定する方法
JP2014507141A (ja) * 2011-02-09 2014-03-27 ナテラ, インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
WO2014026032A2 (en) * 2012-08-08 2014-02-13 Apprise Bio, Inc. Increasing dynamic range for identifying multiple epitopes in cells
WO2014099744A1 (en) * 2012-12-17 2014-06-26 President And Fellows Of Harvard College Rna-guided human genome engineering

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NATURE BIOTECHNOLOGY, vol. 20, JPN6015053064, 2002, pages 473 - 477, ISSN: 0004132563 *
NUCLEIC ACIDS RESEARCH, 2005, VOL.33, NO.18 E162,P.1-7, JPN6019039275, ISSN: 0004298051 *

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