JP2017536548A5 - - Google Patents
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- Publication number
- JP2017536548A5 JP2017536548A5 JP2017527719A JP2017527719A JP2017536548A5 JP 2017536548 A5 JP2017536548 A5 JP 2017536548A5 JP 2017527719 A JP2017527719 A JP 2017527719A JP 2017527719 A JP2017527719 A JP 2017527719A JP 2017536548 A5 JP2017536548 A5 JP 2017536548A5
- Authority
- JP
- Japan
- Prior art keywords
- group
- binding molecule
- modified binding
- chemical ligation
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000004132 cross linking Methods 0.000 claims description 31
- 239000000126 substance Substances 0.000 claims description 29
- 238000003776 cleavage reaction Methods 0.000 claims description 12
- 230000007017 scission Effects 0.000 claims description 12
- 230000004962 physiological condition Effects 0.000 claims description 4
- 150000001540 azides Chemical class 0.000 claims description 3
- 150000002009 diols Chemical group 0.000 claims description 3
- 125000006501 nitrophenyl group Chemical group 0.000 claims description 3
- 125000003831 tetrazolyl group Chemical group 0.000 claims description 3
- 150000007970 thio esters Chemical class 0.000 claims description 3
- 238000000034 method Methods 0.000 claims 24
- 229920001223 polyethylene glycol Polymers 0.000 claims 10
- 125000005843 halogen group Chemical group 0.000 claims 6
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 claims 4
- 108091034117 Oligonucleotide Proteins 0.000 claims 4
- 239000002202 Polyethylene glycol Substances 0.000 claims 4
- 125000002009 alkene group Chemical group 0.000 claims 4
- 125000002355 alkine group Chemical group 0.000 claims 4
- 239000003054 catalyst Substances 0.000 claims 4
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims 4
- 125000003827 glycol group Chemical group 0.000 claims 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims 4
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 claims 4
- 239000003638 chemical reducing agent Substances 0.000 claims 3
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 claims 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims 2
- VMQMZMRVKUZKQL-UHFFFAOYSA-N Cu+ Chemical compound [Cu+] VMQMZMRVKUZKQL-UHFFFAOYSA-N 0.000 claims 2
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims 2
- 239000004472 Lysine Substances 0.000 claims 2
- 241000872931 Myoporum sandwicense Species 0.000 claims 2
- 239000003153 chemical reaction reagent Substances 0.000 claims 2
- AVXURJPOCDRRFD-UHFFFAOYSA-N hydroxylamine group Chemical group NO AVXURJPOCDRRFD-UHFFFAOYSA-N 0.000 claims 2
- 229920000642 polymer Polymers 0.000 claims 2
- QEDXSHCYPROEOK-UHFFFAOYSA-N 3-phosphanylpropanoic acid Chemical compound OC(=O)CCP QEDXSHCYPROEOK-UHFFFAOYSA-N 0.000 claims 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims 1
- 238000006736 Huisgen cycloaddition reaction Methods 0.000 claims 1
- 102000003992 Peroxidases Human genes 0.000 claims 1
- PZBFGYYEXUXCOF-UHFFFAOYSA-N TCEP Chemical compound OC(=O)CCP(CCC(O)=O)CCC(O)=O PZBFGYYEXUXCOF-UHFFFAOYSA-N 0.000 claims 1
- 239000007983 Tris buffer Substances 0.000 claims 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 claims 1
- 229960002685 biotin Drugs 0.000 claims 1
- 235000020958 biotin Nutrition 0.000 claims 1
- 239000011616 biotin Substances 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 238000006243 chemical reaction Methods 0.000 claims 1
- 229910052802 copper Inorganic materials 0.000 claims 1
- 239000010949 copper Substances 0.000 claims 1
- 238000010511 deprotection reaction Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 238000001917 fluorescence detection Methods 0.000 claims 1
- 108040007629 peroxidase activity proteins Proteins 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 238000005406 washing Methods 0.000 claims 1
- 150000001412 amines Chemical class 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462084452P | 2014-11-25 | 2014-11-25 | |
| US62/084,452 | 2014-11-25 | ||
| US201562116962P | 2015-02-17 | 2015-02-17 | |
| US62/116,962 | 2015-02-17 | ||
| PCT/EP2015/077484 WO2016083364A1 (en) | 2014-11-25 | 2015-11-24 | Proximity assays using chemical ligation and hapten transfer |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017536548A JP2017536548A (ja) | 2017-12-07 |
| JP2017536548A5 true JP2017536548A5 (enExample) | 2020-01-16 |
| JP6909156B2 JP6909156B2 (ja) | 2021-07-28 |
Family
ID=54697581
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017527719A Active JP6909156B2 (ja) | 2014-11-25 | 2015-11-24 | 化学ライゲーション及びハプテン移行を用いた近接アッセイ |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US10620217B2 (enExample) |
| EP (1) | EP3224621B1 (enExample) |
| JP (1) | JP6909156B2 (enExample) |
| CN (1) | CN107003323B (enExample) |
| AU (1) | AU2015352625B2 (enExample) |
| CA (1) | CA2965872C (enExample) |
| DK (1) | DK3224621T3 (enExample) |
| WO (1) | WO2016083364A1 (enExample) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107923915B (zh) | 2015-08-28 | 2021-05-04 | 文塔纳医疗系统公司 | 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定 |
| EP4220164B1 (en) * | 2016-12-19 | 2024-08-21 | Ventana Medical Systems, Inc. | Methods and systems for quantitative immunohistochemistry |
| GB2562721A (en) | 2017-05-16 | 2018-11-28 | Fastbase Solutions Ltd | Kits, methods and their uses for detecting cell-cell interactions in a sample |
| US10871485B2 (en) | 2018-04-13 | 2020-12-22 | Rarecyte, Inc. | Kits for labeling of biomarkers and methods of using the same |
| WO2019241191A1 (en) * | 2018-06-11 | 2019-12-19 | Merck Sharp & Dohme Corp. | Cut vertex method for identifying complex molecule substructures |
| US12385923B2 (en) | 2018-11-15 | 2025-08-12 | Arizona Board Of Regents On Behalf Of Arizona State University | Cleavable fluorescent tyramide for sensitive and multiplexed analysis of biological samples |
| CN116868054A (zh) * | 2021-01-25 | 2023-10-10 | 文塔纳医疗系统公司 | 包括用一个或多个可检测部分标记的一种或多种生物标志物的染色生物学样本 |
| CN113552362B (zh) * | 2021-07-23 | 2024-08-09 | 湖北百奥斯生物科技有限公司 | 一种新型信号放大的免疫荧光试剂盒 |
| WO2023058624A1 (ja) * | 2021-10-08 | 2023-04-13 | コニカミノルタ株式会社 | 染色方法、評価方法および標本 |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4711955A (en) | 1981-04-17 | 1987-12-08 | Yale University | Modified nucleotides and methods of preparing and using same |
| US5266684A (en) | 1988-05-02 | 1993-11-30 | The Reagents Of The University Of California | Peptide mixtures |
| CH683667A5 (fr) | 1992-02-26 | 1994-04-29 | Nestle Sa | Matière première aromatique. |
| US5654419A (en) | 1994-02-01 | 1997-08-05 | The Regents Of The University Of California | Fluorescent labels and their use in separations |
| DE69619825T2 (de) | 1995-06-21 | 2002-10-10 | Martek Biosciences Corp., Columbia | Kombinatorische bibliotheken aus markierten biochemischen verbindungen und verfahren zu ihrer herstellung |
| US5661028A (en) | 1995-09-29 | 1997-08-26 | Lockheed Martin Energy Systems, Inc. | Large scale DNA microsequencing device |
| US6444421B1 (en) * | 1997-11-19 | 2002-09-03 | The United States Of America As Represented By The Department Of Health And Human Services | Methods for detecting intermolecular interactions in vivo and in vitro |
| EP1681983A4 (en) * | 2003-10-14 | 2008-12-10 | Monogram Biosciences Inc | RECEPTOR TYROSINE KINASE SIGNAL PATH ANALYSIS FOR DIAGNOSIS AND THERAPY |
| AU2006239154A1 (en) | 2005-04-28 | 2006-11-02 | Ventana Medical Systems, Inc | Nanoparticle conjugates |
| EP3144675A1 (en) | 2005-04-28 | 2017-03-22 | Ventana Medical Systems, Inc. | Enzymes conjugated to antibodies via a peg heterobifuctional linker |
| DK2963011T3 (en) | 2005-11-23 | 2018-08-06 | Ventana Med Syst Inc | MOLECULAR CONJUGATE |
| DK3564674T3 (da) | 2006-11-01 | 2021-10-18 | Ventana Med Syst Inc | Haptener, haptenkonjugater, sammensætninger deraf og fremgangsmåde til fremstilling og anvendelse deraf |
| US20080220434A1 (en) * | 2007-02-07 | 2008-09-11 | Perscitus Biosciences, Llc | Detection Of Molecule Proximity |
| BRPI0816289B1 (pt) | 2007-09-04 | 2019-01-15 | Sanofi Aventis | método para identificar um composto que module uma interação proteína-proteína entre uma primeira proteína e uma segunda proteína e sistema de ensaio |
| JP2012515353A (ja) * | 2009-01-15 | 2012-07-05 | ラボラトリー コーポレイション オブ アメリカ ホールディングス | Ｈｅｒ−２発現の測定による患者の反応を判定する方法 |
| CN101620233B (zh) * | 2009-05-27 | 2012-10-31 | 华中科技大学 | 一种蛋白质相互作用的检测方法 |
| US20120129248A1 (en) * | 2009-07-31 | 2012-05-24 | Prognosys Biosciences, Inc. | Assay tools and methods of use |
| GB0918712D0 (en) * | 2009-10-26 | 2009-12-09 | Axis Shield Diagnostics Ltd | An assay |
| WO2013148498A1 (en) | 2012-03-27 | 2013-10-03 | Ventana Medical Systems, Inc. | Signaling conjugates and methods of use |
| AU2014230945B2 (en) * | 2013-03-12 | 2019-07-11 | Ventana Medical Systems, Inc. | Proximity assay for in situ detection of targets |
| CN103245789B (zh) * | 2013-04-27 | 2014-10-29 | 深圳先进技术研究院 | 用于肿瘤病理诊断的试剂盒及对组织切片进行染色的方法 |
-
2015
- 2015-11-24 AU AU2015352625A patent/AU2015352625B2/en active Active
- 2015-11-24 JP JP2017527719A patent/JP6909156B2/ja active Active
- 2015-11-24 WO PCT/EP2015/077484 patent/WO2016083364A1/en not_active Ceased
- 2015-11-24 CN CN201580063800.0A patent/CN107003323B/zh active Active
- 2015-11-24 EP EP15798449.3A patent/EP3224621B1/en active Active
- 2015-11-24 CA CA2965872A patent/CA2965872C/en active Active
- 2015-11-24 DK DK15798449.3T patent/DK3224621T3/da active
-
2017
- 2017-05-23 US US15/603,079 patent/US10620217B2/en active Active
-
2020
- 2020-03-04 US US16/809,290 patent/US11808770B2/en active Active
-
2023
- 2023-09-27 US US18/373,328 patent/US20240069034A1/en active Pending
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