CA2965872C - Proximity assays using chemical ligation and hapten transfer - Google Patents

Proximity assays using chemical ligation and hapten transfer Download PDF

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Publication number
CA2965872C
CA2965872C CA2965872A CA2965872A CA2965872C CA 2965872 C CA2965872 C CA 2965872C CA 2965872 A CA2965872 A CA 2965872A CA 2965872 A CA2965872 A CA 2965872A CA 2965872 C CA2965872 C CA 2965872C
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Canada
Prior art keywords
chemical ligation
target
bridge component
group
antibody
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Active
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CA2965872A
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English (en)
French (fr)
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CA2965872A1 (en
Inventor
Christopher Bieniarz
Rui Hong
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Ventana Medical Systems Inc
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Ventana Medical Systems Inc
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Publication of CA2965872A1 publication Critical patent/CA2965872A1/en
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54353—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals with ligand attached to the carrier via a chemical coupling agent
    • G01N33/5759—
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2440/00—Post-translational modifications [PTMs] in chemical analysis of biological material

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2965872A 2014-11-25 2015-11-24 Proximity assays using chemical ligation and hapten transfer Active CA2965872C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201462084452P 2014-11-25 2014-11-25
US62/084,452 2014-11-25
US201562116962P 2015-02-17 2015-02-17
US62/116,962 2015-02-17
PCT/EP2015/077484 WO2016083364A1 (en) 2014-11-25 2015-11-24 Proximity assays using chemical ligation and hapten transfer

Publications (2)

Publication Number Publication Date
CA2965872A1 CA2965872A1 (en) 2016-06-02
CA2965872C true CA2965872C (en) 2022-02-22

Family

ID=54697581

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2965872A Active CA2965872C (en) 2014-11-25 2015-11-24 Proximity assays using chemical ligation and hapten transfer

Country Status (8)

Country Link
US (3) US10620217B2 (enExample)
EP (1) EP3224621B1 (enExample)
JP (1) JP6909156B2 (enExample)
CN (1) CN107003323B (enExample)
AU (1) AU2015352625B2 (enExample)
CA (1) CA2965872C (enExample)
DK (1) DK3224621T3 (enExample)
WO (1) WO2016083364A1 (enExample)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107923915B (zh) 2015-08-28 2021-05-04 文塔纳医疗系统公司 使用经掩蔽的半抗原在福尔马林固定石蜡包埋的组织中的蛋白质邻近测定
WO2018118786A1 (en) * 2016-12-19 2018-06-28 Ventana Medical Systems, Inc. Methods and systems for quantitative immunohistochemistry
GB2562721A (en) * 2017-05-16 2018-11-28 Fastbase Solutions Ltd Kits, methods and their uses for detecting cell-cell interactions in a sample
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
CN112567465B (zh) * 2018-06-11 2024-02-20 默沙东有限责任公司 复杂分子子结构的识别系统、装置和方法
WO2020102094A1 (en) * 2018-11-15 2020-05-22 Arizona Board Of Regents On Behalf Ofarizona State University Cleavable fluorescent tyramide for sensitive and multiplexed analysis of biological samples
EP4281779A1 (en) * 2021-01-25 2023-11-29 Ventana Medical Systems, Inc. Stained biological specimens including one or more biomarkers labeled with one or more detectable moieties
CN113552362B (zh) * 2021-07-23 2024-08-09 湖北百奥斯生物科技有限公司 一种新型信号放大的免疫荧光试剂盒
WO2023058624A1 (ja) * 2021-10-08 2023-04-13 コニカミノルタ株式会社 染色方法、評価方法および標本

Family Cites Families (21)

* Cited by examiner, † Cited by third party
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US4711955A (en) 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
US5266684A (en) 1988-05-02 1993-11-30 The Reagents Of The University Of California Peptide mixtures
CH683667A5 (fr) 1992-02-26 1994-04-29 Nestle Sa Matière première aromatique.
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
JPH11509086A (ja) 1995-06-21 1999-08-17 マーテック・バイオサイエンシズ・コーポレイション 標識生化学化合物の組合わせライブラリーとその作成方法
US5661028A (en) 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
US6444421B1 (en) * 1997-11-19 2002-09-03 The United States Of America As Represented By The Department Of Health And Human Services Methods for detecting intermolecular interactions in vivo and in vitro
US7402399B2 (en) * 2003-10-14 2008-07-22 Monogram Biosciences, Inc. Receptor tyrosine kinase signaling pathway analysis for diagnosis and therapy
EP3144675A1 (en) 2005-04-28 2017-03-22 Ventana Medical Systems, Inc. Enzymes conjugated to antibodies via a peg heterobifuctional linker
EP1893241A2 (en) 2005-04-28 2008-03-05 Ventana Medical Systems, Inc. Fluorescent nanoparticles conjugated to antibodies via a peg linker
CN101535244B (zh) 2005-11-23 2014-07-23 文塔納医疗系统公司 分子缀合物
ES2465465T3 (es) 2006-11-01 2014-06-05 Ventana Medical Systems, Inc. Haptenos, conjugados de haptenos, composiciones de los mismos y método para su preparación y uso
US20080220434A1 (en) * 2007-02-07 2008-09-11 Perscitus Biosciences, Llc Detection Of Molecule Proximity
BRPI0816289B1 (pt) 2007-09-04 2019-01-15 Sanofi Aventis método para identificar um composto que module uma interação proteína-proteína entre uma primeira proteína e uma segunda proteína e sistema de ensaio
CA2749817A1 (en) 2009-01-15 2010-07-22 Laboratory Corporation Of America Holdings Methods of determining patient response by measurement of her-2 expression
CN101620233B (zh) * 2009-05-27 2012-10-31 华中科技大学 一种蛋白质相互作用的检测方法
WO2011014879A2 (en) 2009-07-31 2011-02-03 Prognosys Biosciences, Inc. Assay tools and methods of use
GB0918712D0 (en) 2009-10-26 2009-12-09 Axis Shield Diagnostics Ltd An assay
WO2013148498A1 (en) 2012-03-27 2013-10-03 Ventana Medical Systems, Inc. Signaling conjugates and methods of use
AU2014230945B2 (en) * 2013-03-12 2019-07-11 Ventana Medical Systems, Inc. Proximity assay for in situ detection of targets
CN103245789B (zh) * 2013-04-27 2014-10-29 深圳先进技术研究院 用于肿瘤病理诊断的试剂盒及对组织切片进行染色的方法

Also Published As

Publication number Publication date
CN107003323A (zh) 2017-08-01
US20240069034A1 (en) 2024-02-29
AU2015352625A1 (en) 2017-04-20
WO2016083364A1 (en) 2016-06-02
CN107003323B (zh) 2019-06-04
DK3224621T3 (da) 2019-08-12
AU2015352625B2 (en) 2019-07-25
US11808770B2 (en) 2023-11-07
US20170254813A1 (en) 2017-09-07
US20200271662A1 (en) 2020-08-27
JP6909156B2 (ja) 2021-07-28
EP3224621A1 (en) 2017-10-04
JP2017536548A (ja) 2017-12-07
CA2965872A1 (en) 2016-06-02
US10620217B2 (en) 2020-04-14
EP3224621B1 (en) 2019-06-12

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