JP2017535258A5 - - Google Patents
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- Publication number
- JP2017535258A5 JP2017535258A5 JP2017519646A JP2017519646A JP2017535258A5 JP 2017535258 A5 JP2017535258 A5 JP 2017535258A5 JP 2017519646 A JP2017519646 A JP 2017519646A JP 2017519646 A JP2017519646 A JP 2017519646A JP 2017535258 A5 JP2017535258 A5 JP 2017535258A5
- Authority
- JP
- Japan
- Prior art keywords
- site
- exogenous
- goi
- nucleic acid
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 150000007523 nucleic acids Chemical group 0.000 claims description 56
- 238000000034 method Methods 0.000 claims description 42
- 108090000623 proteins and genes Proteins 0.000 claims description 36
- 108020004707 nucleic acids Proteins 0.000 claims description 34
- 102000039446 nucleic acids Human genes 0.000 claims description 34
- 210000004027 cell Anatomy 0.000 claims description 32
- 239000002773 nucleotide Substances 0.000 claims description 32
- 125000003729 nucleotide group Chemical group 0.000 claims description 32
- 230000006798 recombination Effects 0.000 claims description 26
- 238000005215 recombination Methods 0.000 claims description 26
- 239000013598 vector Substances 0.000 claims description 25
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 22
- 102000004169 proteins and genes Human genes 0.000 claims description 21
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims description 19
- 239000012634 fragment Substances 0.000 claims description 18
- 102000018120 Recombinases Human genes 0.000 claims description 16
- 108010091086 Recombinases Proteins 0.000 claims description 16
- 239000000427 antigen Substances 0.000 claims description 14
- 108091007433 antigens Proteins 0.000 claims description 14
- 102000036639 antigens Human genes 0.000 claims description 14
- 108010017070 Zinc Finger Nucleases Proteins 0.000 claims description 12
- 101710163270 Nuclease Proteins 0.000 claims description 9
- 239000003550 marker Substances 0.000 claims description 8
- 108020001507 fusion proteins Proteins 0.000 claims description 6
- 102000037865 fusion proteins Human genes 0.000 claims description 6
- 108020004999 messenger RNA Proteins 0.000 claims description 6
- 238000010459 TALEN Methods 0.000 claims description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims description 4
- 108010008532 Deoxyribonuclease I Proteins 0.000 claims description 4
- 102000007260 Deoxyribonuclease I Human genes 0.000 claims description 4
- 108010073062 Transcription Activator-Like Effectors Proteins 0.000 claims description 4
- 239000000539 dimer Substances 0.000 claims description 4
- 241000702421 Dependoparvovirus Species 0.000 claims description 3
- 102100034343 Integrase Human genes 0.000 claims description 3
- 108010061833 Integrases Proteins 0.000 claims description 3
- 241000713666 Lentivirus Species 0.000 claims description 3
- 102000008579 Transposases Human genes 0.000 claims description 3
- 108010020764 Transposases Proteins 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 239000013612 plasmid Substances 0.000 claims description 3
- 239000000758 substrate Substances 0.000 claims description 3
- 241000701161 unidentified adenovirus Species 0.000 claims description 3
- 241001430294 unidentified retrovirus Species 0.000 claims description 3
- 239000013603 viral vector Substances 0.000 claims description 3
- 230000010354 integration Effects 0.000 claims 3
- 239000012636 effector Substances 0.000 claims 1
- 229920001184 polypeptide Polymers 0.000 claims 1
- 102000004196 processed proteins & peptides Human genes 0.000 claims 1
- 108090000765 processed proteins & peptides Proteins 0.000 claims 1
- 230000002103 transcriptional effect Effects 0.000 claims 1
- 230000002708 enhancing effect Effects 0.000 description 6
- 108060003951 Immunoglobulin Proteins 0.000 description 3
- 102000018358 immunoglobulin Human genes 0.000 description 3
- 108091033319 polynucleotide Proteins 0.000 description 3
- 102000040430 polynucleotide Human genes 0.000 description 3
- 239000002157 polynucleotide Substances 0.000 description 3
- 102000009109 Fc receptors Human genes 0.000 description 2
- 108010087819 Fc receptors Proteins 0.000 description 2
- 108091006020 Fc-tagged proteins Proteins 0.000 description 2
- 239000003446 ligand Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462067774P | 2014-10-23 | 2014-10-23 | |
| US62/067,774 | 2014-10-23 | ||
| PCT/US2015/056653 WO2016064999A1 (en) | 2014-10-23 | 2015-10-21 | Novel cho integration sites and uses thereof |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017535258A JP2017535258A (ja) | 2017-11-30 |
| JP2017535258A5 true JP2017535258A5 (OSRAM) | 2018-11-22 |
| JP6668340B2 JP6668340B2 (ja) | 2020-03-18 |
Family
ID=54602002
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017519646A Active JP6668340B2 (ja) | 2014-10-23 | 2015-10-21 | 新規ｃｈｏ組込み部位およびその使用 |
Country Status (16)
| Country | Link |
|---|---|
| US (5) | US9816110B2 (OSRAM) |
| EP (1) | EP3209785B1 (OSRAM) |
| JP (1) | JP6668340B2 (OSRAM) |
| KR (1) | KR102243243B1 (OSRAM) |
| CN (1) | CN107109434A (OSRAM) |
| AR (1) | AR102420A1 (OSRAM) |
| AU (1) | AU2015335921B2 (OSRAM) |
| BR (1) | BR112017008022A2 (OSRAM) |
| CA (1) | CA2965495C (OSRAM) |
| EA (1) | EA037255B1 (OSRAM) |
| IL (1) | IL251674B (OSRAM) |
| MX (1) | MX2017005306A (OSRAM) |
| MY (1) | MY184103A (OSRAM) |
| SG (1) | SG11201703149RA (OSRAM) |
| TW (2) | TWI780847B (OSRAM) |
| WO (1) | WO2016064999A1 (OSRAM) |
Families Citing this family (38)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2015335921B2 (en) | 2014-10-23 | 2020-06-25 | Regeneron Pharmaceuticals, Inc. | Novel CHO integration sites and uses thereof |
| US11649449B2 (en) | 2015-09-03 | 2023-05-16 | CHO Plus, Inc. | Hybrid cell lines for high level production of a target protein |
| US10329594B1 (en) | 2015-09-03 | 2019-06-25 | CHO Plus, Inc. | Cell lines for high level production of protein-based pharmaceuticals |
| BR112018071283A2 (pt) | 2016-04-20 | 2019-02-12 | Regeneron Pharma | célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno. |
| CA3015371A1 (en) | 2016-04-20 | 2017-10-26 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| WO2018039499A1 (en) | 2016-08-24 | 2018-03-01 | Regeneron Pharmaceuticals, Inc. | Host cell protein modification |
| EP3559236A4 (en) * | 2016-12-20 | 2020-11-04 | Development Center for Biotechnology | TARGETED INTEGRATION SITES IN THE CHINESE HAMSTER OVARIAN CELL GENOME |
| WO2018148196A1 (en) * | 2017-02-07 | 2018-08-16 | Sigma-Aldrich Co. Llc | Stable targeted integration |
| KR20240154089A (ko) * | 2017-02-17 | 2024-10-24 | 론자 리미티드 | 아데노-관련 바이러스 생산을 위한 포유동물 세포 |
| WO2018175153A1 (en) * | 2017-03-19 | 2018-09-27 | Applied Stemcell, Inc. | Novel integration sites and uses thereof |
| US11560549B2 (en) | 2017-08-11 | 2023-01-24 | Boehringer Ingelheim International Gmbh | Integration sites in CHO cells |
| CN107723276B (zh) * | 2017-11-02 | 2021-08-13 | 上海交通大学 | 一种稳定高表达目标产物的细胞株的构建方法和试剂盒 |
| EP3728581B1 (en) * | 2017-12-22 | 2024-10-09 | Bio-Rad Laboratories, Inc. | Controlling phenotype of organisms with crispr/cas gene targeting |
| IL275462B1 (en) * | 2017-12-22 | 2025-09-01 | Genentech Inc | Targeted integration of nucleic acids |
| CN109295092B (zh) * | 2018-10-30 | 2021-06-29 | 江南大学 | 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用 |
| CN109295093B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用 |
| CN109136193B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用 |
| CN109207432B (zh) * | 2018-10-30 | 2021-08-03 | 江南大学 | 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用 |
| CN109337927B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用 |
| CN109321604B (zh) * | 2018-10-30 | 2021-07-06 | 江南大学 | 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用 |
| CN113631711B (zh) * | 2018-12-21 | 2025-03-04 | 豪夫迈·罗氏有限公司 | 核酸的靶向整合 |
| WO2020204055A1 (ja) | 2019-04-02 | 2020-10-08 | 中外製薬株式会社 | 標的特異的な外来遺伝子の導入方法 |
| CN114080454A (zh) | 2019-06-26 | 2022-02-22 | 豪夫迈·罗氏有限公司 | 核酸的随机化构型靶向整合 |
| EP3901266A1 (en) * | 2020-04-22 | 2021-10-27 | LEK Pharmaceuticals d.d. | Super-enhancers for recombinant gene expression in cho cells |
| CN116134136A (zh) * | 2020-06-02 | 2023-05-16 | 康泰伦特药物解决方案有限责任公司 | 具有多个用于基因插入的Dock的细胞系 |
| TW202216991A (zh) * | 2020-06-24 | 2022-05-01 | 美商建南德克公司 | 核酸之靶向整合 |
| EP4229205B1 (en) | 2020-10-15 | 2025-05-14 | F. Hoffmann-La Roche AG | Nucleic acid constructs for va rna transcription |
| CN113755447B (zh) * | 2021-09-23 | 2022-04-29 | 云舟生物科技(广州)股份有限公司 | 一种用于生产腺病毒的293a细胞株及其制备与应用 |
| AU2022371200A1 (en) | 2021-10-18 | 2024-05-02 | Regeneron Pharmaceuticals, Inc. | Controlled transcription of polynucleotides |
| TW202323529A (zh) | 2021-10-18 | 2023-06-16 | 美商再生元醫藥公司 | 包括腺病毒相關病毒聚核苷酸的真核細胞 |
| CA3235566A1 (en) | 2021-10-18 | 2023-04-27 | Michael Goren | Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites |
| IL313321A (en) * | 2022-01-28 | 2024-08-01 | Univ Columbia | Genetic modifications for xenotransplantation |
| CN117305334B (zh) * | 2022-06-21 | 2024-11-08 | 深圳太力生物技术有限责任公司 | 靶向整合细胞及其制备方法、生产目标基因表达产物的方法 |
| TW202434888A (zh) | 2022-11-18 | 2024-09-01 | 美商再生元醫藥公司 | 用於偵測及測定包括生物流體在內之流體中之蛋白質結構及穩定性的方法 |
| CN118726475B (zh) * | 2023-03-28 | 2025-07-08 | 深圳太力生物技术有限责任公司 | 核酸、重组载体、靶向整合细胞、基因表达方法和应用 |
| WO2024211287A1 (en) * | 2023-04-03 | 2024-10-10 | Seagen Inc. | Production cell lines with targeted integration sites |
| WO2025054526A1 (en) | 2023-09-07 | 2025-03-13 | Regeneron Pharmaceuticals, Inc. | Production and purification of covalently surface modified adeno-associated virus |
| WO2025111473A1 (en) | 2023-11-21 | 2025-05-30 | Regeneron Pharmaceuticals, Inc. | Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4634665A (en) | 1980-02-25 | 1987-01-06 | The Trustees Of Columbia University In The City Of New York | Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials |
| US4656134A (en) | 1982-01-11 | 1987-04-07 | Board Of Trustees Of Leland Stanford Jr. University | Gene amplification in eukaryotic cells |
| US6689606B2 (en) | 1998-07-21 | 2004-02-10 | M.L. Laboratories Plc | Polynucleotide |
| US6521419B1 (en) * | 1998-09-22 | 2003-02-18 | Kanakaraju Koduri | Expression vectors containing hot spot for increased recombinant protein expression in transfected cells |
| WO2002081632A2 (en) | 2001-04-04 | 2002-10-17 | The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services | Nucleic acids for transgene expression |
| US20040045043A1 (en) * | 2002-05-20 | 2004-03-04 | Finney Robert E. | Compositions and methods for generating conditional knockouts |
| US8673589B2 (en) | 2002-05-29 | 2014-03-18 | Regeneron Pharmaceuticals, Inc. | Inducible eukaryotic expression system |
| WO2008151219A1 (en) * | 2007-06-04 | 2008-12-11 | Regeneron Pharmaceuticals, Inc. | Enhanced expression and stability regions |
| ES2627552T3 (es) * | 2008-12-04 | 2017-07-28 | Sigma Aldrich Company | Edición de genoma en ratas usando nucleasas con dedos de cinc |
| EP2694661A1 (en) * | 2011-04-05 | 2014-02-12 | The Scripps Research Institute | Chromosomal landing pads and related uses |
| CA2833636A1 (en) | 2011-04-20 | 2012-10-26 | Amplimmune, Inc. | Antibodies and other molecules that bind b7-h1 and pd-1 |
| DK2714936T3 (en) * | 2011-06-01 | 2019-03-25 | Prec Biosciences Inc | METHODS AND PRODUCTS FOR PRODUCING MANIPULATED MAMMAL CELL LINES WITH AMPLIFIED TRANSGENES |
| AU2012328682B2 (en) * | 2011-10-27 | 2017-09-21 | Sangamo Therapeutics, Inc. | Methods and compositions for modification of the HPRT locus |
| EP2847338B1 (en) | 2012-05-07 | 2018-09-19 | Sangamo Therapeutics, Inc. | Methods and compositions for nuclease-mediated targeted integration of transgenes |
| AU2015335921B2 (en) | 2014-10-23 | 2020-06-25 | Regeneron Pharmaceuticals, Inc. | Novel CHO integration sites and uses thereof |
-
2015
- 2015-10-21 AU AU2015335921A patent/AU2015335921B2/en active Active
- 2015-10-21 MX MX2017005306A patent/MX2017005306A/es unknown
- 2015-10-21 KR KR1020177012465A patent/KR102243243B1/ko active Active
- 2015-10-21 BR BR112017008022-2A patent/BR112017008022A2/pt not_active Application Discontinuation
- 2015-10-21 JP JP2017519646A patent/JP6668340B2/ja active Active
- 2015-10-21 CA CA2965495A patent/CA2965495C/en active Active
- 2015-10-21 EP EP15797495.7A patent/EP3209785B1/en active Active
- 2015-10-21 CN CN201580069889.1A patent/CN107109434A/zh active Pending
- 2015-10-21 US US14/919,300 patent/US9816110B2/en active Active
- 2015-10-21 SG SG11201703149RA patent/SG11201703149RA/en unknown
- 2015-10-21 EA EA201790698A patent/EA037255B1/ru unknown
- 2015-10-21 WO PCT/US2015/056653 patent/WO2016064999A1/en not_active Ceased
- 2015-10-21 MY MYPI2017701254A patent/MY184103A/en unknown
- 2015-10-22 TW TW110128495A patent/TWI780847B/zh active
- 2015-10-22 TW TW104134716A patent/TWI747808B/zh active
- 2015-10-23 AR ARP150103445A patent/AR102420A1/es unknown
-
2017
- 2017-04-09 IL IL251674A patent/IL251674B/en active IP Right Grant
- 2017-10-13 US US15/783,525 patent/US20180030480A1/en not_active Abandoned
-
2019
- 2019-09-19 US US16/575,506 patent/US11788102B2/en active Active
-
2021
- 2021-02-12 US US17/175,025 patent/US11268109B2/en active Active
-
2023
- 2023-09-06 US US18/461,798 patent/US20240018553A1/en active Pending
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