MY184103A - Novel cho integration sites and uses thereof - Google Patents

Novel cho integration sites and uses thereof

Info

Publication number
MY184103A
MY184103A MYPI2017701254A MYPI2017701254A MY184103A MY 184103 A MY184103 A MY 184103A MY PI2017701254 A MYPI2017701254 A MY PI2017701254A MY PI2017701254 A MYPI2017701254 A MY PI2017701254A MY 184103 A MY184103 A MY 184103A
Authority
MY
Malaysia
Prior art keywords
expression
integration sites
enhanced
eukaryotic
novel cho
Prior art date
Application number
MYPI2017701254A
Other languages
English (en)
Inventor
Ying Shen
Darya Burakov
Gang Chen
James P Fandl
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MY184103(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MY184103A publication Critical patent/MY184103A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Saccharide Compounds (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
MYPI2017701254A 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof MY184103A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (1)

Publication Number Publication Date
MY184103A true MY184103A (en) 2021-03-18

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
MYPI2017701254A MY184103A (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Country Status (16)

Country Link
US (5) US9816110B2 (OSRAM)
EP (1) EP3209785B1 (OSRAM)
JP (1) JP6668340B2 (OSRAM)
KR (1) KR102243243B1 (OSRAM)
CN (1) CN107109434A (OSRAM)
AR (1) AR102420A1 (OSRAM)
AU (1) AU2015335921B2 (OSRAM)
BR (1) BR112017008022A2 (OSRAM)
CA (1) CA2965495C (OSRAM)
EA (1) EA037255B1 (OSRAM)
IL (1) IL251674B (OSRAM)
MX (1) MX2017005306A (OSRAM)
MY (1) MY184103A (OSRAM)
SG (1) SG11201703149RA (OSRAM)
TW (2) TWI780847B (OSRAM)
WO (1) WO2016064999A1 (OSRAM)

Families Citing this family (38)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015335921B2 (en) 2014-10-23 2020-06-25 Regeneron Pharmaceuticals, Inc. Novel CHO integration sites and uses thereof
US11649449B2 (en) 2015-09-03 2023-05-16 CHO Plus, Inc. Hybrid cell lines for high level production of a target protein
US10329594B1 (en) 2015-09-03 2019-06-25 CHO Plus, Inc. Cell lines for high level production of protein-based pharmaceuticals
BR112018071283A2 (pt) 2016-04-20 2019-02-12 Regeneron Pharma célula, conjunto de vetores para expressar uma proteína de ligação a antígeno biespecífica em uma célula, conjunto de vetores, método, e, método para produção de uma proteína de ligação a antígeno.
CA3015371A1 (en) 2016-04-20 2017-10-26 Regeneron Pharmaceuticals, Inc. Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018039499A1 (en) 2016-08-24 2018-03-01 Regeneron Pharmaceuticals, Inc. Host cell protein modification
EP3559236A4 (en) * 2016-12-20 2020-11-04 Development Center for Biotechnology TARGETED INTEGRATION SITES IN THE CHINESE HAMSTER OVARIAN CELL GENOME
WO2018148196A1 (en) * 2017-02-07 2018-08-16 Sigma-Aldrich Co. Llc Stable targeted integration
KR20240154089A (ko) * 2017-02-17 2024-10-24 론자 리미티드 아데노-관련 바이러스 생산을 위한 포유동물 세포
WO2018175153A1 (en) * 2017-03-19 2018-09-27 Applied Stemcell, Inc. Novel integration sites and uses thereof
US11560549B2 (en) 2017-08-11 2023-01-24 Boehringer Ingelheim International Gmbh Integration sites in CHO cells
CN107723276B (zh) * 2017-11-02 2021-08-13 上海交通大学 一种稳定高表达目标产物的细胞株的构建方法和试剂盒
EP3728581B1 (en) * 2017-12-22 2024-10-09 Bio-Rad Laboratories, Inc. Controlling phenotype of organisms with crispr/cas gene targeting
IL275462B1 (en) * 2017-12-22 2025-09-01 Genentech Inc Targeted integration of nucleic acids
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN113631711B (zh) * 2018-12-21 2025-03-04 豪夫迈·罗氏有限公司 核酸的靶向整合
WO2020204055A1 (ja) 2019-04-02 2020-10-08 中外製薬株式会社 標的特異的な外来遺伝子の導入方法
CN114080454A (zh) 2019-06-26 2022-02-22 豪夫迈·罗氏有限公司 核酸的随机化构型靶向整合
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
CN116134136A (zh) * 2020-06-02 2023-05-16 康泰伦特药物解决方案有限责任公司 具有多个用于基因插入的Dock的细胞系
TW202216991A (zh) * 2020-06-24 2022-05-01 美商建南德克公司 核酸之靶向整合
EP4229205B1 (en) 2020-10-15 2025-05-14 F. Hoffmann-La Roche AG Nucleic acid constructs for va rna transcription
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
AU2022371200A1 (en) 2021-10-18 2024-05-02 Regeneron Pharmaceuticals, Inc. Controlled transcription of polynucleotides
TW202323529A (zh) 2021-10-18 2023-06-16 美商再生元醫藥公司 包括腺病毒相關病毒聚核苷酸的真核細胞
CA3235566A1 (en) 2021-10-18 2023-04-27 Michael Goren Mammalian cells comprising integrated cas9 genes to produce stable integration sites, and mammalian cells comprising stable integration sites and other sites
IL313321A (en) * 2022-01-28 2024-08-01 Univ Columbia Genetic modifications for xenotransplantation
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
TW202434888A (zh) 2022-11-18 2024-09-01 美商再生元醫藥公司 用於偵測及測定包括生物流體在內之流體中之蛋白質結構及穩定性的方法
CN118726475B (zh) * 2023-03-28 2025-07-08 深圳太力生物技术有限责任公司 核酸、重组载体、靶向整合细胞、基因表达方法和应用
WO2024211287A1 (en) * 2023-04-03 2024-10-10 Seagen Inc. Production cell lines with targeted integration sites
WO2025054526A1 (en) 2023-09-07 2025-03-13 Regeneron Pharmaceuticals, Inc. Production and purification of covalently surface modified adeno-associated virus
WO2025111473A1 (en) 2023-11-21 2025-05-30 Regeneron Pharmaceuticals, Inc. Covalently surface modified adeno-associated virus production by in vitro conjugation, and purification of covalently surface modified adeno-associated virus

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4634665A (en) 1980-02-25 1987-01-06 The Trustees Of Columbia University In The City Of New York Processes for inserting DNA into eucaryotic cells and for producing proteinaceous materials
US4656134A (en) 1982-01-11 1987-04-07 Board Of Trustees Of Leland Stanford Jr. University Gene amplification in eukaryotic cells
US6689606B2 (en) 1998-07-21 2004-02-10 M.L. Laboratories Plc Polynucleotide
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2002081632A2 (en) 2001-04-04 2002-10-17 The Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services Nucleic acids for transgene expression
US20040045043A1 (en) * 2002-05-20 2004-03-04 Finney Robert E. Compositions and methods for generating conditional knockouts
US8673589B2 (en) 2002-05-29 2014-03-18 Regeneron Pharmaceuticals, Inc. Inducible eukaryotic expression system
WO2008151219A1 (en) * 2007-06-04 2008-12-11 Regeneron Pharmaceuticals, Inc. Enhanced expression and stability regions
ES2627552T3 (es) * 2008-12-04 2017-07-28 Sigma Aldrich Company Edición de genoma en ratas usando nucleasas con dedos de cinc
EP2694661A1 (en) * 2011-04-05 2014-02-12 The Scripps Research Institute Chromosomal landing pads and related uses
CA2833636A1 (en) 2011-04-20 2012-10-26 Amplimmune, Inc. Antibodies and other molecules that bind b7-h1 and pd-1
DK2714936T3 (en) * 2011-06-01 2019-03-25 Prec Biosciences Inc METHODS AND PRODUCTS FOR PRODUCING MANIPULATED MAMMAL CELL LINES WITH AMPLIFIED TRANSGENES
AU2012328682B2 (en) * 2011-10-27 2017-09-21 Sangamo Therapeutics, Inc. Methods and compositions for modification of the HPRT locus
EP2847338B1 (en) 2012-05-07 2018-09-19 Sangamo Therapeutics, Inc. Methods and compositions for nuclease-mediated targeted integration of transgenes
AU2015335921B2 (en) 2014-10-23 2020-06-25 Regeneron Pharmaceuticals, Inc. Novel CHO integration sites and uses thereof

Also Published As

Publication number Publication date
US20200002732A1 (en) 2020-01-02
US20210171984A1 (en) 2021-06-10
KR102243243B1 (ko) 2021-04-22
TWI747808B (zh) 2021-12-01
CN107109434A (zh) 2017-08-29
EA037255B1 (ru) 2021-02-26
MX2017005306A (es) 2018-01-09
AR102420A1 (es) 2017-03-01
US11268109B2 (en) 2022-03-08
IL251674B (en) 2020-10-29
JP2017535258A (ja) 2017-11-30
JP6668340B2 (ja) 2020-03-18
TW202144576A (zh) 2021-12-01
US20160115502A1 (en) 2016-04-28
EP3209785B1 (en) 2019-07-31
AU2015335921B2 (en) 2020-06-25
CA2965495C (en) 2022-05-24
KR20170096999A (ko) 2017-08-25
US20180030480A1 (en) 2018-02-01
EP3209785A1 (en) 2017-08-30
IL251674A0 (en) 2017-06-29
AU2015335921A1 (en) 2017-05-04
TWI780847B (zh) 2022-10-11
SG11201703149RA (en) 2018-02-27
CA2965495A1 (en) 2016-04-28
BR112017008022A2 (pt) 2018-02-20
US11788102B2 (en) 2023-10-17
WO2016064999A1 (en) 2016-04-28
EA201790698A1 (ru) 2018-01-31
TW201629229A (zh) 2016-08-16
US9816110B2 (en) 2017-11-14
US20240018553A1 (en) 2024-01-18

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