JP2017532043A5 - - Google Patents
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- Publication number
- JP2017532043A5 JP2017532043A5 JP2017519813A JP2017519813A JP2017532043A5 JP 2017532043 A5 JP2017532043 A5 JP 2017532043A5 JP 2017519813 A JP2017519813 A JP 2017519813A JP 2017519813 A JP2017519813 A JP 2017519813A JP 2017532043 A5 JP2017532043 A5 JP 2017532043A5
- Authority
- JP
- Japan
- Prior art keywords
- erf1
- combination
- mutant erf1
- mutant
- unnatural amino
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 102100030667 Eukaryotic peptide chain release factor subunit 1 Human genes 0.000 claims 36
- 101710175705 Eukaryotic peptide chain release factor subunit 1 Proteins 0.000 claims 36
- 108020004705 Codon Proteins 0.000 claims 19
- 150000001413 amino acids Chemical class 0.000 claims 17
- 230000035772 mutation Effects 0.000 claims 16
- 210000004027 cell Anatomy 0.000 claims 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims 14
- 150000007523 nucleic acids Chemical group 0.000 claims 13
- 229920001184 polypeptide Polymers 0.000 claims 12
- 102000004196 processed proteins & peptides Human genes 0.000 claims 12
- 238000010348 incorporation Methods 0.000 claims 11
- 238000000034 method Methods 0.000 claims 9
- 108020004707 nucleic acids Proteins 0.000 claims 8
- 102000039446 nucleic acids Human genes 0.000 claims 8
- 102000004169 proteins and genes Human genes 0.000 claims 7
- 108090000623 proteins and genes Proteins 0.000 claims 7
- 210000003527 eukaryotic cell Anatomy 0.000 claims 6
- 108091028043 Nucleic acid sequence Proteins 0.000 claims 5
- 125000003275 alpha amino acid group Chemical group 0.000 claims 5
- 102220498965 Phosphatidylinositol 4-phosphate 5-kinase type-1 beta_Y125F_mutation Human genes 0.000 claims 4
- 102000052866 Amino Acyl-tRNA Synthetases Human genes 0.000 claims 2
- 108700028939 Amino Acyl-tRNA Synthetases Proteins 0.000 claims 2
- 102220473604 Cytochrome b5_M51A_mutation Human genes 0.000 claims 2
- 102220590746 Mediator of RNA polymerase II transcription subunit 13_T58K_mutation Human genes 0.000 claims 2
- 102220558658 Nuclear protein 1_E55A_mutation Human genes 0.000 claims 2
- 102220558654 Nuclear protein 1_E55Q_mutation Human genes 0.000 claims 2
- 102220643725 Prolactin-inducible protein_S60T_mutation Human genes 0.000 claims 2
- 102220530815 Transcription factor SOX-9_S64D_mutation Human genes 0.000 claims 2
- 102220247983 rs761483326 Human genes 0.000 claims 2
- YMBQDEOCKOLCJG-AXDSSHIGSA-N (2S)-2-amino-6-[(2-methylcycloprop-2-en-1-yl)methoxycarbonylamino]hexanoic acid Chemical compound CC1=CC1COC(=O)NCCCC[C@H](N)C(O)=O YMBQDEOCKOLCJG-AXDSSHIGSA-N 0.000 claims 1
- 241000238631 Hexapoda Species 0.000 claims 1
- 102200027218 c.217G>A Human genes 0.000 claims 1
- 210000004962 mammalian cell Anatomy 0.000 claims 1
- 239000011022 opal Substances 0.000 claims 1
- 102200033501 rs387907005 Human genes 0.000 claims 1
- 239000000758 substrate Substances 0.000 claims 1
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1419109.2 | 2014-10-27 | ||
| GBGB1419109.2A GB201419109D0 (en) | 2014-10-27 | 2014-10-27 | Incorporation of unnatural amino acids into proteins |
| PCT/GB2015/053141 WO2016066995A1 (en) | 2014-10-27 | 2015-10-21 | Incorporation of unnatural amino acids into proteins |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177605A Division JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017532043A JP2017532043A (ja) | 2017-11-02 |
| JP2017532043A5 true JP2017532043A5 (enExample) | 2018-10-25 |
| JP6843044B2 JP6843044B2 (ja) | 2021-03-17 |
Family
ID=52103474
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2017519813A Active JP6843044B2 (ja) | 2014-10-27 | 2015-10-21 | 非天然アミノ酸のタンパク質への組み込み |
| JP2020177605A Withdrawn JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2020177605A Withdrawn JP2021035374A (ja) | 2014-10-27 | 2020-10-22 | 非天然アミノ酸のタンパク質への組み込み |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US10738339B2 (enExample) |
| EP (1) | EP3212799B1 (enExample) |
| JP (2) | JP6843044B2 (enExample) |
| KR (1) | KR102516695B1 (enExample) |
| CN (1) | CN107002088B (enExample) |
| BR (1) | BR112017008108A2 (enExample) |
| CA (1) | CA2965085A1 (enExample) |
| GB (1) | GB201419109D0 (enExample) |
| WO (1) | WO2016066995A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104718285A (zh) | 2012-05-18 | 2015-06-17 | 医药研究委员会 | 利用编码包含BCN基团的氨基酸的正交密码子和正交PylRS合成酶将包含BCN基团的氨基酸整合到多肽中的方法 |
| GB2528227A (en) | 2014-03-14 | 2016-01-20 | Medical Res Council | Cyclopropene amino acids and methods |
| WO2018044143A1 (ko) * | 2016-09-05 | 2018-03-08 | 한국과학기술원 | 목적 단백질의 위치 특이적 변형이 시공간적으로 조절되는 마우스, 이의 제조방법 및 그 용도 |
| CN106834349B (zh) * | 2017-02-28 | 2019-12-13 | 中国人民解放军军事医学科学院 | 一种安全性提高的病毒基因递送系统 |
| CN110959041B (zh) * | 2017-06-02 | 2023-09-29 | Ambrx公司 | 促进含非天然氨基酸的蛋白质产生的方法和组合物 |
| WO2019213331A1 (en) * | 2018-05-01 | 2019-11-07 | Ambrx, Inc. | A method for optimizing antibody expression |
| WO2020051331A1 (en) * | 2018-09-07 | 2020-03-12 | Medimmune, Llc | Methods of cell selection |
| GB201817444D0 (en) | 2018-10-26 | 2018-12-12 | Res & Innovation Uk | Methods and compositions |
| EP3696189A1 (en) * | 2019-02-14 | 2020-08-19 | European Molecular Biology Laboratory | Means and methods for preparing engineered target proteins by genetic code expansion in a target protein selective manner |
| CN111718949B (zh) * | 2019-03-19 | 2021-10-01 | 宁波鲲鹏生物科技有限公司 | 利用双质粒系统在蛋白中引入非天然氨基酸 |
| CN111850020B (zh) * | 2019-04-25 | 2021-05-07 | 苏州鲲鹏生物技术有限公司 | 利用质粒系统在蛋白中引入非天然氨基酸 |
| CN111849929B (zh) * | 2019-04-30 | 2021-05-11 | 苏州鲲鹏生物技术有限公司 | 高效引入赖氨酸衍生物的氨酰基—tRNA合成酶 |
| GB201906775D0 (en) | 2019-05-14 | 2019-06-26 | Res & Innovation Uk | Synthetic genome |
| TW202128996A (zh) * | 2019-10-10 | 2021-08-01 | 美商史基普研究協會 | 用於活體內合成非天然多肽的組合物及方法 |
| CN117881779A (zh) | 2021-05-28 | 2024-04-12 | 英国研究与创新署 | 微生物及其用途 |
| CN113481239A (zh) * | 2021-07-01 | 2021-10-08 | 四川大学华西医院 | 一种通过Rosa26位点向细胞系中引入非天然氨基酸编码体系的方法及其细胞系 |
| KR20240032941A (ko) * | 2021-07-07 | 2024-03-12 | 아지노모토 가부시키가이샤 | 비천연 아미노산 함유 단백질의 분비 생산법 |
| GB202110137D0 (en) | 2021-07-14 | 2021-08-25 | Res & Innovation Uk | Methods for optimising protein production |
| CN113699124B (zh) * | 2021-09-08 | 2022-04-12 | 北京大学 | 一种含非天然氨基酸蛋白的制备方法 |
| WO2023196596A2 (en) * | 2022-04-08 | 2023-10-12 | Absci Corporation | Trna synthetases and methods of use thereof |
| WO2024199465A1 (en) * | 2023-03-30 | 2024-10-03 | The Chinese University Of Hong Kong | Unnatural amino acid in cellulo synthesis for site-specific protein modification |
| CN120752342A (zh) * | 2023-07-13 | 2025-10-03 | 深圳华大生命科学研究院 | 工程生物遏制系统及方法 |
| CN120210144A (zh) * | 2023-12-25 | 2025-06-27 | 康码（上海）生物科技有限公司 | 一种改造的氨酰-tRNA合成酶、核酸构建物以及基因工程菌株 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2004321117B2 (en) * | 2003-12-18 | 2010-09-02 | The Scripps Research Institute | Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells |
| MX2007004892A (es) * | 2004-10-27 | 2007-08-16 | Scripps Research Inst | Componentes de traduccion ortogonales para la incorporacion in vivo de aminoacidos no naturales. |
| AU2008301614A1 (en) | 2007-09-20 | 2009-03-26 | Riken | Mutant pyrrolysyl-TRNA synthetase, and method for production of protein having non-natural amino acid integrated therein by using the same |
| GB2470770A (en) | 2009-06-04 | 2010-12-08 | Medical Res Council | Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs |
| US20120077186A1 (en) | 2010-05-07 | 2012-03-29 | Oregon Health & Science University | Use of cysteine-derived suppressor trnas for non-native amino acid incorporation |
| GB201201100D0 (en) | 2012-01-20 | 2012-03-07 | Medical Res Council | Polypeptides and methods |
-
2014
- 2014-10-27 GB GBGB1419109.2A patent/GB201419109D0/en not_active Ceased
-
2015
- 2015-10-21 BR BR112017008108A patent/BR112017008108A2/pt not_active Application Discontinuation
- 2015-10-21 US US15/521,103 patent/US10738339B2/en active Active
- 2015-10-21 WO PCT/GB2015/053141 patent/WO2016066995A1/en not_active Ceased
- 2015-10-21 JP JP2017519813A patent/JP6843044B2/ja active Active
- 2015-10-21 EP EP15794622.9A patent/EP3212799B1/en active Active
- 2015-10-21 CN CN201580058784.6A patent/CN107002088B/zh active Active
- 2015-10-21 CA CA2965085A patent/CA2965085A1/en not_active Abandoned
- 2015-10-21 KR KR1020177014048A patent/KR102516695B1/ko active Active
-
2020
- 2020-10-22 JP JP2020177605A patent/JP2021035374A/ja not_active Withdrawn
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