JP2017515484A5 - - Google Patents

Download PDF

Info

Publication number
JP2017515484A5
JP2017515484A5 JP2016567863A JP2016567863A JP2017515484A5 JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5 JP 2016567863 A JP2016567863 A JP 2016567863A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2017515484 A5 JP2017515484 A5 JP 2017515484A5
Authority
JP
Japan
Prior art keywords
subsequence
sequence
oligonucleotide
blocker
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2016567863A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017515484A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/031476 external-priority patent/WO2015179339A1/en
Publication of JP2017515484A publication Critical patent/JP2017515484A/ja
Publication of JP2017515484A5 publication Critical patent/JP2017515484A5/ja
Pending legal-status Critical Current

Links

JP2016567863A 2014-05-19 2015-05-19 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 Pending JP2017515484A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
US62/000,114 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020165028A Division JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Publications (2)

Publication Number Publication Date
JP2017515484A JP2017515484A (ja) 2017-06-15
JP2017515484A5 true JP2017515484A5 (cg-RX-API-DMAC7.html) 2018-06-28

Family

ID=54554607

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2016567863A Pending JP2017515484A (ja) 2014-05-19 2015-05-19 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2020165028A Active JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2022126459A Pending JP2022166131A (ja) 2014-05-19 2022-08-08 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2024153764A Pending JP2024167406A (ja) 2014-05-19 2024-09-06 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020165028A Active JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2022126459A Pending JP2022166131A (ja) 2014-05-19 2022-08-08 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2024153764A Pending JP2024167406A (ja) 2014-05-19 2024-09-06 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Country Status (13)

Country Link
US (2) US20170067090A1 (cg-RX-API-DMAC7.html)
EP (3) EP3146080B1 (cg-RX-API-DMAC7.html)
JP (4) JP2017515484A (cg-RX-API-DMAC7.html)
KR (1) KR102343605B1 (cg-RX-API-DMAC7.html)
CN (2) CN106661627B (cg-RX-API-DMAC7.html)
AU (2) AU2015264357B2 (cg-RX-API-DMAC7.html)
BR (1) BR112016027063A2 (cg-RX-API-DMAC7.html)
CA (1) CA2985631A1 (cg-RX-API-DMAC7.html)
DK (1) DK3146080T3 (cg-RX-API-DMAC7.html)
ES (1) ES2861353T3 (cg-RX-API-DMAC7.html)
PH (1) PH12016502542B1 (cg-RX-API-DMAC7.html)
RU (1) RU2708992C2 (cg-RX-API-DMAC7.html)
WO (1) WO2015179339A1 (cg-RX-API-DMAC7.html)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
CA2865575C (en) 2012-02-27 2024-01-16 Cellular Research, Inc. Compositions and kits for molecular counting
KR102758333B1 (ko) 2013-08-28 2025-01-23 벡톤 디킨슨 앤드 컴퍼니 대량의 동시 단일 세포 분석
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en) 2013-12-05 2015-06-11 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
KR20230076867A (ko) 2013-12-20 2023-05-31 더 브로드 인스티튜트, 인코퍼레이티드 신생항원 백신과의 병용 요법
EP3234130B1 (en) 2014-12-19 2020-11-25 The Broad Institute, Inc. Methods for profiling the t-cell- receptor repertoire
US10975442B2 (en) 2014-12-19 2021-04-13 Massachusetts Institute Of Technology Molecular biomarkers for cancer immunotherapy
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
WO2016160844A2 (en) 2015-03-30 2016-10-06 Cellular Research, Inc. Methods and compositions for combinatorial barcoding
EP4582556A3 (en) 2015-04-23 2025-10-08 Becton, Dickinson and Company Methods and compositions for whole transcriptome amplification
JP6940484B2 (ja) 2015-09-11 2021-09-29 セルラー リサーチ， インコーポレイテッド ライブラリー正規化のための方法および組成物
CN108603193B (zh) * 2015-12-30 2022-07-22 生物辐射实验室股份有限公司 分裂循环和tape扩增
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
CN109791157B (zh) 2016-09-26 2022-06-07 贝克顿迪金森公司 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达
US11549149B2 (en) * 2017-01-24 2023-01-10 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
CN110382708A (zh) 2017-02-01 2019-10-25 赛卢拉研究公司 使用阻断性寡核苷酸进行选择性扩增
CN110719959B (zh) 2017-06-05 2021-08-06 贝克顿迪金森公司 针对单细胞的样品索引
JP2021500077A (ja) * 2017-10-18 2021-01-07 デイ ゼロ ダイアグノスティックス， インコーポレイテッド Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化
EP3759118A4 (en) * 2018-02-20 2022-03-23 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
CN108220444A (zh) * 2018-02-28 2018-06-29 无锡禾盛医疗器械有限公司 一种kras基因突变检测的引物探针组合及其应用
JP7358388B2 (ja) 2018-05-03 2023-10-10 ベクトン・ディキンソン・アンド・カンパニー 反対側の転写物末端における分子バーコーディング
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
ES2992135T3 (es) 2018-10-01 2024-12-09 Becton Dickinson Co Determinar secuencias de transcripción 5’
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
US11492660B2 (en) 2018-12-13 2022-11-08 Becton, Dickinson And Company Selective extension in single cell whole transcriptome analysis
US20200224260A1 (en) * 2019-01-15 2020-07-16 Tangen Bioscience Inc. Method for suppressing non-specific amplification products in nucleic acid amplification technologies
ES2945227T3 (es) 2019-01-23 2023-06-29 Becton Dickinson Co Oligonucleótidos asociados con anticuerpos
WO2020167920A1 (en) 2019-02-14 2020-08-20 Cellular Research, Inc. Hybrid targeted and whole transcriptome amplification
US20220348606A1 (en) * 2019-06-26 2022-11-03 Nanjing GenScript Biotech Co., Ltd. Oligonucleotide containing blocker
CN120099137A (zh) 2019-07-22 2025-06-06 贝克顿迪金森公司 单细胞染色质免疫沉淀测序测定
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US20240279735A1 (en) * 2019-12-20 2024-08-22 Korea University Research And Business Foundation Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
US11649497B2 (en) 2020-01-13 2023-05-16 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and RNA
WO2021146219A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Cell capture using du-containing oligonucleotides
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
WO2021173719A1 (en) 2020-02-25 2021-09-02 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
US20230220456A1 (en) * 2020-05-01 2023-07-13 William Marsh Rice University Quantitative blocker displacement amplification (qbda) sequencing for calibration-free and multiplexed variant allele frequency quantitation
US11661625B2 (en) 2020-05-14 2023-05-30 Becton, Dickinson And Company Primers for immune repertoire profiling
US12157913B2 (en) 2020-06-02 2024-12-03 Becton, Dickinson And Company Oligonucleotides and beads for 5 prime gene expression assay
CN116075520A (zh) * 2020-06-26 2023-05-05 威廉马歇莱思大学 扩增子全面富集
CN111647650B (zh) * 2020-07-06 2023-06-27 河南赛诺特生物技术有限公司 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
US12391940B2 (en) 2020-07-31 2025-08-19 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022094403A1 (en) * 2020-11-02 2022-05-05 Nuprobe Usa, Inc. Quantitative multiplex amplicon sequencing system
EP4247967A1 (en) 2020-11-20 2023-09-27 Becton, Dickinson and Company Profiling of highly expressed and lowly expressed proteins
CN112522369A (zh) * 2020-12-08 2021-03-19 合肥欧创基因生物科技有限公司 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2022146773A1 (en) 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
WO2022155397A1 (en) 2021-01-15 2022-07-21 Nuprobe Usa, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en) 2021-03-16 2022-09-22 Nuprobe Usa, Inc. High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) * 2021-11-01 2023-05-04 Nuprobe Usa, Inc. Rna quantitative amplicon sequencing for gene expression quantitation
CN119662835B (zh) * 2025-02-20 2025-05-27 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
ATE541055T1 (de) * 2004-10-18 2012-01-15 Univ Brandeis Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation
WO2007106534A2 (en) * 2006-03-14 2007-09-20 Harbor-Ucla Research And Education Institute Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
JP5535921B2 (ja) * 2007-10-04 2014-07-02 コモンウェルス サイエンティフィック アンドインダストリアル リサーチ オーガナイゼーション 核酸増幅
CN105200097A (zh) * 2008-10-20 2015-12-30 霍夫曼-拉罗奇有限公司 改进的等位基因特异性扩增
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
EP2411543B1 (en) * 2009-03-27 2017-04-19 Life Technologies Corporation Methods, compositions, and kits for detecting allelic variants
KR101312241B1 (ko) * 2010-04-27 2013-09-27 사회복지법인 삼성생명공익재단 증폭억제시발체를 이용하는 유전자 돌연변이 검출 방법
US20130059294A1 (en) * 2010-05-16 2013-03-07 Xiangdong Ren Identification of polymorphic hepatitis b viruses and kras oncogene mutations and clinical use
GB2487632B (en) * 2011-01-14 2014-03-12 Genefirst Ltd Methods,compositions,and kits for determining the presence/absence of a variant nucleic acid sequence
KR20210056458A (ko) * 2011-05-04 2021-05-18 바이오셉트 인코포레이티드 핵산 서열 변이체를 검출하는 방법
EP2798078B1 (en) * 2011-12-30 2018-02-14 Polowang Biotech Inc. Method and primer set for detecting mutation
US9879316B2 (en) * 2012-04-25 2018-01-30 Tho Huu Ho Method for competitive allele-specific cDNA synthesis and differential amplification of the cDNA products
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
EP2992110B1 (en) * 2013-04-29 2017-10-25 QIAGEN Marseille S.A.S. A method for dna amplification with a blocking oligonucleotide

Similar Documents

Publication Publication Date Title
JP2017515484A5 (cg-RX-API-DMAC7.html)
JP7126718B2 (ja) 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2024167406A5 (cg-RX-API-DMAC7.html)
JP2015156872A5 (cg-RX-API-DMAC7.html)
JP2017504360A5 (cg-RX-API-DMAC7.html)
Guo et al. A specific and versatile genome walking technique
JP2018514230A5 (cg-RX-API-DMAC7.html)
JP2017537658A5 (cg-RX-API-DMAC7.html)
JP2005270108A5 (cg-RX-API-DMAC7.html)
JP2011507488A5 (cg-RX-API-DMAC7.html)
JP2010519896A5 (cg-RX-API-DMAC7.html)
WO2017218777A1 (en) Nucleic acid reactions and related methods and compositions
IL274638B1 (en) Asymmetric pcr methods
US10801056B2 (en) Kit and method for detection of microRNA
JP2017500020A5 (cg-RX-API-DMAC7.html)
US20160060710A1 (en) Compas-pcr method and methods for detecting, identifying or monitoring salmonid species
CN102382814A (zh) 核酸等温扩增方法
JP2014516545A5 (cg-RX-API-DMAC7.html)
Ableitner “Molbio” in Keywords
JP2015019631A5 (cg-RX-API-DMAC7.html)
JP7306722B2 (ja) プライマー及びその使用
JP4455168B2 (ja) Ｄｎａ増幅方法
JP2023540161A5 (cg-RX-API-DMAC7.html)
HK1236578B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides