JP2017515484A - 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 - Google Patents

重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 Download PDF

Info

Publication number
JP2017515484A
JP2017515484A JP2016567863A JP2016567863A JP2017515484A JP 2017515484 A JP2017515484 A JP 2017515484A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2016567863 A JP2016567863 A JP 2016567863A JP 2017515484 A JP2017515484 A JP 2017515484A
Authority
JP
Japan
Prior art keywords
subsequence
sequence
blocker
oligonucleotide
target
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2016567863A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017515484A5 (cg-RX-API-DMAC7.html
Inventor
デヴィット ユー ツァン
デヴィット ユー ツァン
ルオジャ ウー
ルオジャ ウー
ジュシャオ ワン
ジュシャオ ワン
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
William Marsh Rice University
Original Assignee
William Marsh Rice University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by William Marsh Rice University filed Critical William Marsh Rice University
Publication of JP2017515484A publication Critical patent/JP2017515484A/ja
Publication of JP2017515484A5 publication Critical patent/JP2017515484A5/ja
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/163—Reactions characterised by the reaction format or use of a specific feature the purpose or use of blocking probe

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2016567863A 2014-05-19 2015-05-19 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅 Pending JP2017515484A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462000114P 2014-05-19 2014-05-19
US62/000,114 2014-05-19
PCT/US2015/031476 WO2015179339A1 (en) 2014-05-19 2015-05-19 Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2020165028A Division JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Publications (2)

Publication Number Publication Date
JP2017515484A true JP2017515484A (ja) 2017-06-15
JP2017515484A5 JP2017515484A5 (cg-RX-API-DMAC7.html) 2018-06-28

Family

ID=54554607

Family Applications (4)

Application Number Title Priority Date Filing Date
JP2016567863A Pending JP2017515484A (ja) 2014-05-19 2015-05-19 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2020165028A Active JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2022126459A Pending JP2022166131A (ja) 2014-05-19 2022-08-08 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2024153764A Pending JP2024167406A (ja) 2014-05-19 2024-09-06 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Family Applications After (3)

Application Number Title Priority Date Filing Date
JP2020165028A Active JP7126718B2 (ja) 2014-05-19 2020-09-30 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2022126459A Pending JP2022166131A (ja) 2014-05-19 2022-08-08 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP2024153764A Pending JP2024167406A (ja) 2014-05-19 2024-09-06 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅

Country Status (13)

Country Link
US (2) US20170067090A1 (cg-RX-API-DMAC7.html)
EP (3) EP4512908A3 (cg-RX-API-DMAC7.html)
JP (4) JP2017515484A (cg-RX-API-DMAC7.html)
KR (1) KR102343605B1 (cg-RX-API-DMAC7.html)
CN (2) CN114032234A (cg-RX-API-DMAC7.html)
AU (2) AU2015264357B2 (cg-RX-API-DMAC7.html)
BR (1) BR112016027063A2 (cg-RX-API-DMAC7.html)
CA (1) CA2985631A1 (cg-RX-API-DMAC7.html)
DK (1) DK3146080T3 (cg-RX-API-DMAC7.html)
ES (1) ES2861353T3 (cg-RX-API-DMAC7.html)
PH (1) PH12016502542B1 (cg-RX-API-DMAC7.html)
RU (1) RU2708992C2 (cg-RX-API-DMAC7.html)
WO (1) WO2015179339A1 (cg-RX-API-DMAC7.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021500077A (ja) * 2017-10-18 2021-01-07 デイ ゼロ ダイアグノスティックス， インコーポレイテッド Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化

Families Citing this family (53)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10941396B2 (en) 2012-02-27 2021-03-09 Becton, Dickinson And Company Compositions and kits for molecular counting
EP3039158B1 (en) 2013-08-28 2018-11-14 Cellular Research, Inc. Massively parallel single cell analysis
WO2015077717A1 (en) 2013-11-25 2015-05-28 The Broad Institute Inc. Compositions and methods for diagnosing, evaluating and treating cancer by means of the dna methylation status
WO2015085147A1 (en) 2013-12-05 2015-06-11 The Broad Institute Inc. Polymorphic gene typing and somatic change detection using sequencing data
CA2934073A1 (en) 2013-12-20 2015-06-25 The Broad Institute, Inc. Combination therapy with neoantigen vaccine
EP3234193B1 (en) 2014-12-19 2020-07-15 Massachusetts Institute of Technology Molecular biomarkers for cancer immunotherapy
ES3049406T3 (en) 2014-12-19 2025-12-16 Broad Inst Inc Methods for profiling the t-cell-receptor repertoire
US9727810B2 (en) 2015-02-27 2017-08-08 Cellular Research, Inc. Spatially addressable molecular barcoding
JP7508191B2 (ja) 2015-03-30 2024-07-01 ベクトン・ディキンソン・アンド・カンパニー コンビナトリアルバーコーディングのための方法および組成物
WO2016172373A1 (en) 2015-04-23 2016-10-27 Cellular Research, Inc. Methods and compositions for whole transcriptome amplification
KR102395450B1 (ko) 2015-09-11 2022-05-09 셀룰러 리서치, 인크. 핵산 라이브러리 정규화를 위한 방법 및 조성물
CN108603193B (zh) 2015-12-30 2022-07-22 生物辐射实验室股份有限公司 分裂循环和tape扩增
WO2017193025A1 (en) 2016-05-06 2017-11-09 William Marsh Rice University Stoichiometric nucleic acid purification using randomer capture probe libraries
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
EP3516400B1 (en) 2016-09-26 2023-08-16 Becton, Dickinson and Company Measurement of protein expression using reagents with barcoded oligonucleotide sequences
EP3574116A1 (en) * 2017-01-24 2019-12-04 The Broad Institute, Inc. Compositions and methods for detecting a mutant variant of a polynucleotide
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
US12331350B2 (en) * 2018-02-20 2025-06-17 William Marsh Rice University Systems and methods for allele enrichment using multiplexed blocker displacement amplification
CN108220444A (zh) * 2018-02-28 2018-06-29 无锡禾盛医疗器械有限公司 一种kras基因突变检测的引物探针组合及其应用
CN112272710A (zh) 2018-05-03 2021-01-26 贝克顿迪金森公司 高通量多组学样品分析
CN118910215A (zh) 2018-05-03 2024-11-08 贝克顿迪金森公司 在相对的转录物末端进行分子条形码化
US11639517B2 (en) 2018-10-01 2023-05-02 Becton, Dickinson And Company Determining 5′ transcript sequences
US11932849B2 (en) 2018-11-08 2024-03-19 Becton, Dickinson And Company Whole transcriptome analysis of single cells using random priming
CN113195717A (zh) * 2018-12-13 2021-07-30 贝克顿迪金森公司 单细胞全转录组分析中的选择性延伸
EP3911754A4 (en) * 2019-01-15 2022-10-12 Tangen Biosciences Inc. METHOD FOR REMOVAL OF NON-SPECIFIC AMPLIFICATION PRODUCTS IN NUCLEIC ACID AMPLIFICATION TECHNOLOGIES
EP4242322B1 (en) 2019-01-23 2024-08-21 Becton, Dickinson and Company Oligonucleotides associated with antibodies
US12071617B2 (en) 2019-02-14 2024-08-27 Becton, Dickinson And Company Hybrid targeted and whole transcriptome amplification
US20220348606A1 (en) * 2019-06-26 2022-11-03 Nanjing GenScript Biotech Co., Ltd. Oligonucleotide containing blocker
US11939622B2 (en) 2019-07-22 2024-03-26 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
JP7522189B2 (ja) 2019-11-08 2024-07-24 ベクトン・ディキンソン・アンド・カンパニー 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用
US20240279735A1 (en) * 2019-12-20 2024-08-22 Korea University Research And Business Foundation Primers for detecting trace amount of rare single- nucleotide variant and method for specifically and sensitively detecting trace amount of rare single-nucleotide variant by using same
WO2021146207A1 (en) 2020-01-13 2021-07-22 Becton, Dickinson And Company Methods and compositions for quantitation of proteins and rna
ES2993319T3 (en) 2020-01-29 2024-12-27 Becton Dickinson Co Barcoded wells for spatial mapping of single cells through sequencing
CN115151810A (zh) 2020-02-25 2022-10-04 贝克顿迪金森公司 实现使用单细胞样品作为单色补偿对照的双特异性探针
CN115605614A (zh) 2020-05-14 2023-01-13 贝克顿迪金森公司（Us） 用于免疫组库谱分析的引物
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
CN116075520A (zh) * 2020-06-26 2023-05-05 威廉马歇莱思大学 扩增子全面富集
CN111647650B (zh) * 2020-07-06 2023-06-27 河南赛诺特生物技术有限公司 检测人B-raf基因V600E突变的引物、引物探针组合物及试剂盒
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
CN116547390A (zh) * 2020-11-02 2023-08-04 阅尔基因美国公司 定量多重扩增子测序系统
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN112522369A (zh) * 2020-12-08 2021-03-19 合肥欧创基因生物科技有限公司 一种ARMS-TaqMan Blocker体系的Blocker双链设计方法
US12392771B2 (en) 2020-12-15 2025-08-19 Becton, Dickinson And Company Single cell secretome analysis
WO2022146773A1 (en) 2020-12-29 2022-07-07 Nuprobe Usa, Inc. Methods and compositions for sequencing and fusion detection using ligation tail adapters (lta)
EP4278009A1 (en) 2021-01-15 2023-11-22 Nuprobe USA, Inc. Cycle multiplexing for highly multiplexed quantitative pcr
WO2022197688A1 (en) 2021-03-16 2022-09-22 Nuprobe Usa, Inc. High sensitivity detection of microsatellite loci by blocker displacement amplification with multiple blockers and its use in microsatellite instability detection
WO2023077121A1 (en) * 2021-11-01 2023-05-04 Nuprobe Usa, Inc. Rna quantitative amplicon sequencing for gene expression quantitation
CN120624641A (zh) * 2025-02-20 2025-09-12 宁波熙宁检测技术有限公司 一种用于检测ezh2基因突变的核酸组合物、试剂盒及检测方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149695A1 (en) * 2010-04-27 2013-06-13 Samsung Life Public Welfare Foundation Method for detecting genetic mutation by using a blocking primer
WO2013097173A1 (en) * 2011-12-30 2013-07-04 Mackay Memorial Hospital Method and primer set for detecting mutation

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6312892B1 (en) * 1996-07-19 2001-11-06 Cornell Research Foundation, Inc. High fidelity detection of nucleic acid differences by ligase detection reaction
BRPI0516619B1 (pt) * 2004-10-18 2016-02-10 Univ Brandeis reagentes, kit de reagentes e conjunto de oligonucleotídeos para realização de uma amplificação por reação em cadeia de polimerase
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
CN101889096B (zh) * 2007-10-04 2015-10-21 联邦科学及工业研究组织 核酸扩增
ES2527653T3 (es) * 2008-10-20 2015-01-28 F. Hoffmann-La Roche Ag Amplificación específica de alelo usando un cebador con un nucleótido modificado
US20100285478A1 (en) * 2009-03-27 2010-11-11 Life Technologies Corporation Methods, Compositions, and Kits for Detecting Allelic Variants
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
CN102985562A (zh) * 2010-05-16 2013-03-20 润尼生命科学公司 鉴别多态性乙肝病毒和kras致癌基因突变及其临床应用
EP2663651B1 (en) * 2011-01-14 2018-06-20 Genefirst Limited Methods, compositions, and kits for determing the presence/absence of a variant nucleic acid sequence
CN103635593B (zh) * 2011-05-04 2018-03-20 生物概念股份有限公司 用于检测核酸序列变体的方法
CA2871704C (en) * 2012-04-25 2022-10-18 Tho Huu HO Method for competitive allele-specific cdna synthesis and differential amplification of the cdna products
CN103215361A (zh) * 2013-04-18 2013-07-24 深圳联合医学科技有限公司 等位基因变体检测方法、试剂盒及组合物
EP2992110B1 (en) * 2013-04-29 2017-10-25 QIAGEN Marseille S.A.S. A method for dna amplification with a blocking oligonucleotide

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20130149695A1 (en) * 2010-04-27 2013-06-13 Samsung Life Public Welfare Foundation Method for detecting genetic mutation by using a blocking primer
WO2013097173A1 (en) * 2011-12-30 2013-07-04 Mackay Memorial Hospital Method and primer set for detecting mutation

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
BIOTECHNIQUES, vol. 37, no. 2, JPN6019026124, 2004, pages 226 - 231, ISSN: 0004270336 *
JAPANESE JOURNAL OF APPLIED PHYSICS, vol. Vol. 45, No. 1B, JPN6019026125, 2006, pages 509 - 512, ISSN: 0004270337 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2021500077A (ja) * 2017-10-18 2021-01-07 デイ ゼロ ダイアグノスティックス， インコーポレイテッド Ｄｎａ増幅の標的化抑制による混合ｄｎａ試料中のｄｎａ集団の選択的富化
US11795485B2 (en) 2017-10-18 2023-10-24 Day Zero Diagnostics, Inc. Selective enrichment of a population of DNA in a mixed DNA sample through targeted suppression of DNA amplification

Also Published As

Publication number Publication date
RU2016149307A3 (cg-RX-API-DMAC7.html) 2018-12-10
EP3146080B1 (en) 2021-01-06
AU2015264357A1 (en) 2017-01-05
RU2708992C2 (ru) 2019-12-12
AU2021266291B2 (en) 2024-09-19
EP4512908A2 (en) 2025-02-26
JP7126718B2 (ja) 2022-08-29
DK3146080T3 (en) 2021-04-12
EP3146080A4 (en) 2017-11-01
AU2021266291A1 (en) 2021-12-09
PH12016502542B1 (en) 2024-01-17
RU2016149307A (ru) 2018-06-20
EP3901278B1 (en) 2024-10-23
JP2022166131A (ja) 2022-11-01
CN114032234A (zh) 2022-02-11
CN106661627A (zh) 2017-05-10
AU2021266291C1 (en) 2024-12-12
CA2985631A1 (en) 2015-11-26
PH12016502542A1 (en) 2017-04-10
KR102343605B1 (ko) 2021-12-27
JP2024167406A (ja) 2024-12-03
EP4512908A3 (en) 2025-03-26
US20220090168A1 (en) 2022-03-24
EP3146080A1 (en) 2017-03-29
EP3901278A1 (en) 2021-10-27
WO2015179339A1 (en) 2015-11-26
AU2015264357B2 (en) 2021-08-12
ES2861353T3 (es) 2021-10-06
BR112016027063A2 (pt) 2017-10-31
KR20170003695A (ko) 2017-01-09
CN106661627B (zh) 2021-11-23
JP2021019607A (ja) 2021-02-18
US20170067090A1 (en) 2017-03-09

Similar Documents

Publication Publication Date Title
JP7126718B2 (ja) 重複する非対立遺伝子特異的プライマー及び対立遺伝子特異的ブロッカーオリゴヌクレオチドの組成物を用いる対立遺伝子特異的な増幅
JP4417723B2 (ja) ヘッドループｄｎａの増幅
US12275990B2 (en) Modified primers for nucleic acid amplification and detection
JP6940412B2 (ja) Ｐｃｒ方法
WO2017138484A1 (ja) 標的核酸を検出する方法およびそれに用いる核酸プローブ
Fakruddin et al. Competitiveness of polymerase chain reaction to alternate amplification methods
EP3377652A1 (en) A method and a kit for nucleic acid detection
HK40065933A (en) Allele-specific amplification using overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578A1 (en) Allele-specific amplification using a composition of overlapping non-allele-specific primer and allele-specific blocker oligonucleotides
HK1236578B (zh) 使用重叠非等位基因特异性引物和等位基因特异性阻断剂寡核苷酸的组合物进行等位基因特异性扩增
JP2018533943A (ja) 一塩基多型検出用キット及び方法
JP4406366B2 (ja) 多型配列部位を有する核酸の識別方法
Whitcombe 6 Using Scorpion Primers for Genotyping
JP2007330101A (ja) 核酸の増幅方法
JP2005278443A (ja) オリゴヌクレオチド及びそれを用いた淋菌の検出方法
EP3377651A1 (en) A method and a kit for nucleic acid detection

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20180518

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20180518

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20190708

A601 Written request for extension of time

Free format text: JAPANESE INTERMEDIATE CODE: A601

Effective date: 20191007

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20191204

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20200601