JP2017509350A - ガイドrnaの生成のための方法および組成物 - Google Patents
ガイドrnaの生成のための方法および組成物 Download PDFInfo
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- JP2017509350A JP2017509350A JP2016560684A JP2016560684A JP2017509350A JP 2017509350 A JP2017509350 A JP 2017509350A JP 2016560684 A JP2016560684 A JP 2016560684A JP 2016560684 A JP2016560684 A JP 2016560684A JP 2017509350 A JP2017509350 A JP 2017509350A
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- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N2310/12—Type of nucleic acid catalytic nucleic acids, e.g. ribozymes
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
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- C12N2830/00—Vector systems having a special element relevant for transcription
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- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/80—Vector systems having a special element relevant for transcription from vertebrates
- C12N2830/85—Vector systems having a special element relevant for transcription from vertebrates mammalian
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461974672P | 2014-04-03 | 2014-04-03 | |
| US61/974,672 | 2014-04-03 | ||
| PCT/US2015/024196 WO2015153940A1 (en) | 2014-04-03 | 2015-04-03 | Methods and compositions for the production of guide rna |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JP2017509350A true JP2017509350A (ja) | 2017-04-06 |
| JP2017509350A5 JP2017509350A5 (enExample) | 2018-05-31 |
Family
ID=52997563
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2016560684A Pending JP2017509350A (ja) | 2014-04-03 | 2015-04-03 | ガイドrnaの生成のための方法および組成物 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20170022499A1 (enExample) |
| EP (1) | EP3126503A1 (enExample) |
| JP (1) | JP2017509350A (enExample) |
| CN (1) | CN106170550A (enExample) |
| WO (1) | WO2015153940A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017502683A (ja) * | 2014-01-14 | 2017-01-26 | ラム セラピューティクス, インコーポレイテッド | 変異誘発方法 |
| JP2023532864A (ja) * | 2020-06-30 | 2023-08-01 | ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ エジンバラ | 導入遺伝子発現系 |
Families Citing this family (117)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| US10378027B2 (en) | 2013-03-15 | 2019-08-13 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9359599B2 (en) | 2013-08-22 | 2016-06-07 | President And Fellows Of Harvard College | Engineered transcription activator-like effector (TALE) domains and uses thereof |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| WO2015066119A1 (en) | 2013-10-30 | 2015-05-07 | North Carolina State University | Compositions and methods related to a type-ii crispr-cas system in lactobacillus buchneri |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| KR20160102056A (ko) * | 2013-12-26 | 2016-08-26 | 더 제너럴 하스피탈 코포레이션 | 멀티플렉스 가이드 rna |
| US10787654B2 (en) | 2014-01-24 | 2020-09-29 | North Carolina State University | Methods and compositions for sequence guiding Cas9 targeting |
| EP3129490A4 (en) | 2014-04-08 | 2017-10-04 | North Carolina State University | Methods and compositions for rna-directed repression of transcription using crispr-associated genes |
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| WO2016084084A1 (en) * | 2014-11-27 | 2016-06-02 | Danziger Innovations Ltd. | Nucleic acid constructs for genome editing |
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| WO2016196361A1 (en) | 2015-05-29 | 2016-12-08 | North Carolina State University | Methods for screening bacteria, archaea, algae, and yeast using crispr nucleic acids |
| JP7051438B2 (ja) | 2015-06-15 | 2022-04-11 | ノース カロライナ ステート ユニバーシティ | 核酸およびrnaに基づく抗菌剤の効率的な送達のための方法および組成物 |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
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| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| WO2017106414A1 (en) * | 2015-12-18 | 2017-06-22 | Danisco Us Inc. | Methods and compositions for polymerase ii (pol-ii) based guide rna expression |
| WO2017112620A1 (en) | 2015-12-22 | 2017-06-29 | North Carolina State University | Methods and compositions for delivery of crispr based antimicrobials |
| CN105543223A (zh) * | 2015-12-25 | 2016-05-04 | 华侨大学 | 一种基于miRNA/shRNA转录加工机制转录sgRNA的方法 |
| EP3199632A1 (en) | 2016-01-26 | 2017-08-02 | ACIB GmbH | Temperature-inducible crispr/cas system |
| EP3219799A1 (en) | 2016-03-17 | 2017-09-20 | IMBA-Institut für Molekulare Biotechnologie GmbH | Conditional crispr sgrna expression |
| JP2019514377A (ja) | 2016-04-26 | 2019-06-06 | マサチューセッツ インスティテュート オブ テクノロジー | 拡張可能なリコンビナーゼカスケード |
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| EP3568476A1 (en) * | 2017-01-11 | 2019-11-20 | Oxford University Innovation Limited | Crispr rna |
| TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
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| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| EP3638789A4 (en) * | 2017-06-12 | 2021-03-10 | California Institute of Technology | CONDITIONAL GUIDE RNAS |
| US9982279B1 (en) | 2017-06-23 | 2018-05-29 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| US10011849B1 (en) | 2017-06-23 | 2018-07-03 | Inscripta, Inc. | Nucleic acid-guided nucleases |
| HUE058668T2 (hu) | 2017-06-30 | 2022-09-28 | Inscripta Inc | Automatizált sejtkezelési eljárások, modulok, berendezések és rendszerek |
| US11041154B2 (en) | 2017-07-21 | 2021-06-22 | Arizona Board Of Regents On Behalf Of Arizona State University | CRISPR fluorescent guide RNA (fgRNA) to understanding gRNAs expressed from pol II promotors |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| CA3111479A1 (en) * | 2017-09-26 | 2019-04-04 | The Board Of Trustees Of The University Of Illinois | Crispr/cas system and method for genome editing and modulating transcription |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| EP3765612A4 (en) * | 2018-03-12 | 2022-01-05 | Nanjing Bioheng Biotech Co., Ltd | MODIFIED CHEMERICAL GUIDE RNA AND ITS USES |
| US10760075B2 (en) | 2018-04-30 | 2020-09-01 | Snipr Biome Aps | Treating and preventing microbial infections |
| CN108330138B (zh) * | 2018-03-29 | 2018-09-25 | 上海欣百诺生物科技有限公司 | 一种sgRNA的体外合成方法及其试剂盒 |
| CN108728441B (zh) * | 2018-04-18 | 2022-07-22 | 深圳市第二人民医院 | 特异性识别p53突变的基因系统 |
| US10858761B2 (en) | 2018-04-24 | 2020-12-08 | Inscripta, Inc. | Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells |
| US10508273B2 (en) | 2018-04-24 | 2019-12-17 | Inscripta, Inc. | Methods for identifying selective binding pairs |
| US10526598B2 (en) | 2018-04-24 | 2020-01-07 | Inscripta, Inc. | Methods for identifying T-cell receptor antigens |
| WO2019213065A1 (en) * | 2018-04-30 | 2019-11-07 | Oregon Health & Science University | Methods of gene therapy |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| AU2019292919A1 (en) | 2018-06-30 | 2021-03-11 | Inscripta, Inc. | Instruments, modules, and methods for improved detection of edited sequences in live cells |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
| WO2020072253A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for screening for variant cells |
| WO2020072250A1 (en) | 2018-10-01 | 2020-04-09 | North Carolina State University | Recombinant type i crispr-cas system and uses thereof for genome modification and alteration of expression |
| US10711267B2 (en) | 2018-10-01 | 2020-07-14 | North Carolina State University | Recombinant type I CRISPR-Cas system |
| US12264330B2 (en) | 2018-10-01 | 2025-04-01 | North Carolina State University | Recombinant type I CRISPR-Cas system and uses thereof for killing target cells |
| US11851663B2 (en) | 2018-10-14 | 2023-12-26 | Snipr Biome Aps | Single-vector type I vectors |
| GB201817010D0 (en) * | 2018-10-18 | 2018-12-05 | Imperial Innovations Ltd | Methods |
| US11214781B2 (en) | 2018-10-22 | 2022-01-04 | Inscripta, Inc. | Engineered enzyme |
| EP3870697A4 (en) | 2018-10-22 | 2022-11-09 | Inscripta, Inc. | GMO ENZYMES |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US20220056460A1 (en) * | 2018-12-05 | 2022-02-24 | Dsm Ip Assets B.V. | Crispr guide-rna expression strategies for multiplex genome engineering |
| WO2020131986A1 (en) * | 2018-12-21 | 2020-06-25 | Pioneer Hi-Bred International, Inc. | Multiplex genome targeting |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| EP3942043A2 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US11001831B2 (en) | 2019-03-25 | 2021-05-11 | Inscripta, Inc. | Simultaneous multiplex genome editing in yeast |
| CN113631713A (zh) * | 2019-03-25 | 2021-11-09 | 因思科瑞普特公司 | 酵母中的同时多重基因组编辑 |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US10837021B1 (en) | 2019-06-06 | 2020-11-17 | Inscripta, Inc. | Curing for recursive nucleic acid-guided cell editing |
| WO2020257395A1 (en) | 2019-06-21 | 2020-12-24 | Inscripta, Inc. | Genome-wide rationally-designed mutations leading to enhanced lysine production in e. coli |
| US10927385B2 (en) | 2019-06-25 | 2021-02-23 | Inscripta, Inc. | Increased nucleic-acid guided cell editing in yeast |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021102059A1 (en) | 2019-11-19 | 2021-05-27 | Inscripta, Inc. | Methods for increasing observed editing in bacteria |
| KR20250033331A (ko) | 2019-12-10 | 2025-03-07 | 인스크립타 인코포레이티드 | 신규 mad 뉴클레아제 |
| CA3157127A1 (en) | 2019-12-18 | 2021-06-24 | Aamir MIR | Cascade/dcas3 complementation assays for in vivo detection of nucleic acid-guided nuclease edited cells |
| KR20220133257A (ko) | 2020-01-27 | 2022-10-04 | 인스크립타 인코포레이티드 | 전기천공 모듈 및 기구 |
| EP3889259A1 (en) | 2020-03-30 | 2021-10-06 | IMBA-Institut für Molekulare Biotechnologie GmbH | Internal standard for crispr guide rna |
| US20210332388A1 (en) | 2020-04-24 | 2021-10-28 | Inscripta, Inc. | Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| US11787841B2 (en) | 2020-05-19 | 2023-10-17 | Inscripta, Inc. | Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli |
| GB202007943D0 (en) | 2020-05-27 | 2020-07-08 | Snipr Biome Aps | Products & methods |
| CN111850011B (zh) * | 2020-06-24 | 2022-08-26 | 湖南文理学院 | 改良的Csy4序列、改良方法及应用 |
| WO2022060749A1 (en) | 2020-09-15 | 2022-03-24 | Inscripta, Inc. | Crispr editing to embed nucleic acid landing pads into genomes of live cells |
| US11512297B2 (en) | 2020-11-09 | 2022-11-29 | Inscripta, Inc. | Affinity tag for recombination protein recruitment |
| CA3204158A1 (en) | 2021-01-04 | 2022-07-07 | Juhan Kim | Mad nucleases |
| US11332742B1 (en) | 2021-01-07 | 2022-05-17 | Inscripta, Inc. | Mad nucleases |
| US11873485B2 (en) | 2021-01-26 | 2024-01-16 | California Institute Of Technology | Allosteric conditional guide RNAs for cell-selective regulation of CRISPR/Cas |
| US11884924B2 (en) | 2021-02-16 | 2024-01-30 | Inscripta, Inc. | Dual strand nucleic acid-guided nickase editing |
| WO2022204088A1 (en) * | 2021-03-22 | 2022-09-29 | Massachusetts Institute Of Technology | Multi-input mirna sensing with constitutive erns to regulate multi-output gene expression in mammalian cells |
| EP4326863A4 (en) * | 2021-04-20 | 2025-04-23 | Texas Tech University System | Tissue culture-independent gene editing of cells using a long-distance RNA delivery system |
| CN115704040A (zh) * | 2021-08-06 | 2023-02-17 | 华东理工大学 | 基于CRISPRi和CRISPRa的转录调控系统、其建立方法及应用 |
| WO2023018938A1 (en) * | 2021-08-12 | 2023-02-16 | The J. David Gladstone Institutes, A Testamentary Trust Established Under The Will Of J. David Gladstone | Methods for generation of precise rna transcripts |
| CN113604472B (zh) * | 2021-09-17 | 2024-01-09 | 中国科学院植物研究所 | 一种应用于里氏木霉的CRISPR/Cas基因编辑系统 |
| GB202209518D0 (en) | 2022-06-29 | 2022-08-10 | Snipr Biome Aps | Treating & preventing E coli infections |
| WO2025165779A1 (en) * | 2024-02-01 | 2025-08-07 | The Board Of Trustees Of The Leland Stanford Junior University | Simultaneous gene knockin and knockout in engineered cell therapies with mrna expressed guide rnas |
| CN119220682B (zh) * | 2024-10-25 | 2025-04-22 | 首都医科大学附属北京儿童医院 | Saal1作为神经母细胞瘤预后评估标志物和药物靶点的应用 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| LT3401400T (lt) * | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
| JP2015527889A (ja) * | 2012-07-25 | 2015-09-24 | ザ ブロード インスティテュート, インコーポレイテッド | 誘導可能なdna結合タンパク質およびゲノム撹乱ツール、ならびにそれらの適用 |
| CN103388006B (zh) * | 2013-07-26 | 2015-10-28 | 华东师范大学 | 一种基因定点突变的构建方法 |
| KR20160102056A (ko) * | 2013-12-26 | 2016-08-26 | 더 제너럴 하스피탈 코포레이션 | 멀티플렉스 가이드 rna |
-
2015
- 2015-04-03 EP EP15718044.9A patent/EP3126503A1/en not_active Withdrawn
- 2015-04-03 US US15/301,135 patent/US20170022499A1/en not_active Abandoned
- 2015-04-03 CN CN201580018277.XA patent/CN106170550A/zh active Pending
- 2015-04-03 WO PCT/US2015/024196 patent/WO2015153940A1/en not_active Ceased
- 2015-04-03 JP JP2016560684A patent/JP2017509350A/ja active Pending
Non-Patent Citations (4)
| Title |
|---|
| BIOTECHNOL. LETT., vol. 33, JPN6019017332, 2011, pages 1723 - 1728, ISSN: 0004033975 * |
| CELL, vol. 152, no. 5, JPN6019017334, 2013, pages 1173 - 1183, ISSN: 0004033973 * |
| MOL. CELL, vol. 54, no. 4, JPN6019017339, 22 May 2014 (2014-05-22), pages 698 - 710, ISSN: 0004033974 * |
| NAT. BIOTECHNOL., vol. 23, no. 3, JPN6019017336, 2005, pages 337 - 343, ISSN: 0004033976 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2017502683A (ja) * | 2014-01-14 | 2017-01-26 | ラム セラピューティクス, インコーポレイテッド | 変異誘発方法 |
| JP2023532864A (ja) * | 2020-06-30 | 2023-08-01 | ザ ユニバーシティー コート オブ ザ ユニバーシティー オブ エジンバラ | 導入遺伝子発現系 |
Also Published As
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| CN106170550A (zh) | 2016-11-30 |
| US20170022499A1 (en) | 2017-01-26 |
| EP3126503A1 (en) | 2017-02-08 |
| WO2015153940A1 (en) | 2015-10-08 |
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