CN108330138B - 一种sgRNA的体外合成方法及其试剂盒 - Google Patents
一种sgRNA的体外合成方法及其试剂盒 Download PDFInfo
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- CN108330138B CN108330138B CN201810634615.8A CN201810634615A CN108330138B CN 108330138 B CN108330138 B CN 108330138B CN 201810634615 A CN201810634615 A CN 201810634615A CN 108330138 B CN108330138 B CN 108330138B
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- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/00—Structure or type of the nucleic acid
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- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
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Abstract
Description
Claims (11)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810271411 | 2018-03-29 | ||
CN2018102714112 | 2018-03-29 |
Publications (2)
Publication Number | Publication Date |
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CN108330138A CN108330138A (zh) | 2018-07-27 |
CN108330138B true CN108330138B (zh) | 2018-09-25 |
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ID=62934957
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201810634615.8A Active CN108330138B (zh) | 2018-03-29 | 2018-06-20 | 一种sgRNA的体外合成方法及其试剂盒 |
Country Status (3)
Country | Link |
---|---|
US (1) | US20210115437A1 (zh) |
CN (1) | CN108330138B (zh) |
WO (1) | WO2019185034A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108998483B (zh) * | 2018-08-17 | 2021-04-30 | 武汉核圣生物技术有限公司 | 利用单亚基RNA聚合酶进行体外转录合成sgRNA的方法 |
CN111518838A (zh) * | 2020-05-01 | 2020-08-11 | 山东瑞辑通慧生物技术有限公司 | 一种真核细胞单碱基基因编辑的引物、试剂盒及使用方法、用途 |
CN112981544A (zh) * | 2021-02-25 | 2021-06-18 | 通用生物系统(安徽)有限公司 | 一种sgRNA文库构建方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2015153940A1 (en) * | 2014-04-03 | 2015-10-08 | Massachusetts Institute Of Technology | Methods and compositions for the production of guide rna |
WO2016201047A1 (en) * | 2015-06-09 | 2016-12-15 | Editas Medicine, Inc. | Crispr/cas-related methods and compositions for improving transplantation |
US10301619B2 (en) * | 2016-04-01 | 2019-05-28 | New England Biolabs, Inc. | Compositions and methods relating to synthetic RNA polynucleotides created from synthetic DNA oligonucleotides |
CN105861552B (zh) * | 2016-04-25 | 2019-10-11 | 西北农林科技大学 | 一种T7 RNA聚合酶介导的CRISPR/Cas9基因编辑系统的构建方法 |
CN107058573B (zh) * | 2016-06-13 | 2020-07-10 | 艾吉泰康生物科技(北京)有限公司 | 一种利用Cas9/gRNA系统构建扩增子文库的方法 |
WO2018005691A1 (en) * | 2016-06-29 | 2018-01-04 | The Regents Of The University Of California | Efficient genetic screening method |
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2018
- 2018-06-20 CN CN201810634615.8A patent/CN108330138B/zh active Active
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2019
- 2019-03-29 WO PCT/CN2019/080485 patent/WO2019185034A1/zh active Application Filing
- 2019-03-29 US US17/043,119 patent/US20210115437A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
CN108330138A (zh) | 2018-07-27 |
US20210115437A1 (en) | 2021-04-22 |
WO2019185034A1 (zh) | 2019-10-03 |
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Effective date of registration: 20191009 Address after: 215200 4th floor, No.228 yunchuang Road, Wujiang Economic and Technological Development Zone, Wujiang District, Suzhou City, Jiangsu Province Patentee after: Wujiang Novoprotein Technology Co., Ltd. Address before: 201203 Pudong New Area, Shanghai, China (Shanghai) free trade trial zone, 304 lane, 2 lane, 720 lane, Cai Lun Road. Patentee before: Sinobio Biotech Co. |
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Address after: 215200 4th floor, 228 yunchuang Road, Wujiang Economic and Technological Development Zone, Wujiang District, Suzhou City, Jiangsu Province Patentee after: Suzhou inshore protein Technology Co.,Ltd. Address before: 215200 4th floor, 228 yunchuang Road, Wujiang Economic and Technological Development Zone, Wujiang District, Suzhou City, Jiangsu Province Patentee before: WUJIANG NOVOPROTEIN SCIENTIFIC Inc. |
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