JP2017505127A5 - - Google Patents

Download PDF

Info

Publication number
JP2017505127A5
JP2017505127A5 JP2016548101A JP2016548101A JP2017505127A5 JP 2017505127 A5 JP2017505127 A5 JP 2017505127A5 JP 2016548101 A JP2016548101 A JP 2016548101A JP 2016548101 A JP2016548101 A JP 2016548101A JP 2017505127 A5 JP2017505127 A5 JP 2017505127A5
Authority
JP
Japan
Prior art keywords
segment
cell
locus
cells
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2016548101A
Other languages
English (en)
Japanese (ja)
Other versions
JP6479024B2 (ja
JP2017505127A (ja
Filing date
Publication date
Application filed filed Critical
Priority claimed from PCT/US2015/012577 external-priority patent/WO2015112790A2/en
Publication of JP2017505127A publication Critical patent/JP2017505127A/ja
Publication of JP2017505127A5 publication Critical patent/JP2017505127A5/ja
Application granted granted Critical
Publication of JP6479024B2 publication Critical patent/JP6479024B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2016548101A 2014-01-24 2015-01-23 抗体親和性の最適化のための高スループットマウスモデル Active JP6479024B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461931074P 2014-01-24 2014-01-24
US61/931,074 2014-01-24
PCT/US2015/012577 WO2015112790A2 (en) 2014-01-24 2015-01-23 High-throughput mouse model for optimizing antibody affinities

Publications (3)

Publication Number Publication Date
JP2017505127A JP2017505127A (ja) 2017-02-16
JP2017505127A5 true JP2017505127A5 (https=) 2018-03-01
JP6479024B2 JP6479024B2 (ja) 2019-03-06

Family

ID=53682124

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016548101A Active JP6479024B2 (ja) 2014-01-24 2015-01-23 抗体親和性の最適化のための高スループットマウスモデル

Country Status (4)

Country Link
US (2) US10034463B2 (https=)
EP (1) EP3096609B1 (https=)
JP (1) JP6479024B2 (https=)
WO (1) WO2015112790A2 (https=)

Families Citing this family (46)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6482757B2 (ja) 2010-07-26 2019-03-13 トリアンニ インコーポレイテッドＴｒｉａｎｎｉ，Ｉｎｃ． トランスジェニック動物および使用方法
US10793829B2 (en) 2010-07-26 2020-10-06 Trianni, Inc. Transgenic mammals and methods of use thereof
US10662256B2 (en) 2010-07-26 2020-05-26 Trianni, Inc. Transgenic mammals and methods of use thereof
JP6261500B2 (ja) 2011-07-22 2018-01-17 プレジデント アンド フェローズ オブ ハーバード カレッジ ヌクレアーゼ切断特異性の評価および改善
SI2986729T1 (sl) 2013-04-16 2019-02-28 Regeneron Pharmaceuticals, Inc. Ciljana sprememba genoma podgane
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9340799B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College MRNA-sensing switchable gRNAs
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
AU2014346559B2 (en) 2013-11-07 2020-07-09 Editas Medicine,Inc. CRISPR-related methods and compositions with governing gRNAs
US9546384B2 (en) 2013-12-11 2017-01-17 Regeneron Pharmaceuticals, Inc. Methods and compositions for the targeted modification of a mouse genome
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
US10034463B2 (en) * 2014-01-24 2018-07-31 Children's Medical Center Corporation High-throughput mouse model for optimizing antibody affinities
WO2016022363A2 (en) 2014-07-30 2016-02-11 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
ES2731437T3 (es) 2014-11-21 2019-11-15 Regeneron Pharma Métodos y composiciones para la modificación genética dirigida mediante el uso de pares de ARN guías
JP7109784B2 (ja) 2015-10-23 2022-08-01 プレジデント アンド フェローズ オブ ハーバード カレッジ 遺伝子編集のための進化したＣａｓ９蛋白質
EP3384030A4 (en) 2015-12-03 2019-07-03 Trianni, Inc. IMPROVED IMMUNOGLULINIVITY
AU2017213633B2 (en) 2016-02-04 2023-08-03 Trianni, Inc. Enhanced production of immunoglobulins
CN118185874A (zh) * 2016-04-04 2024-06-14 苏黎世联邦理工学院 一种重组哺乳动物b细胞
KR102547316B1 (ko) 2016-08-03 2023-06-23 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 아데노신 핵염기 편집제 및 그의 용도
US11661590B2 (en) 2016-08-09 2023-05-30 President And Fellows Of Harvard College Programmable CAS9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
EP3526320A1 (en) 2016-10-14 2019-08-21 President and Fellows of Harvard College Aav delivery of nucleobase editors
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
JP2020510038A (ja) 2017-03-09 2020-04-02 プレジデント アンド フェローズ オブ ハーバード カレッジ がんワクチン
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
JP2020510439A (ja) 2017-03-10 2020-04-09 プレジデント アンド フェローズ オブ ハーバード カレッジ シトシンからグアニンへの塩基編集因子
CN110637083A (zh) * 2017-03-20 2019-12-31 华盛顿大学 细胞及其使用和制备方法
CA3057192A1 (en) 2017-03-23 2018-09-27 President And Fellows Of Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
CA3060935A1 (en) 2017-05-01 2018-11-08 The Children's Medical Center Coporation Methods and compositions relating to anti-pd1 antibody reagents
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
WO2019023680A1 (en) 2017-07-28 2019-01-31 President And Fellows Of Harvard College METHODS AND COMPOSITIONS FOR EVOLUTION OF BASIC EDITORS USING PHAGE-ASSISTED CONTINUOUS EVOLUTION (PACE)
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
WO2019118949A1 (en) 2017-12-15 2019-06-20 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
EP3797160A1 (en) 2018-05-23 2021-03-31 The Broad Institute Inc. Base editors and uses thereof
US20210238312A1 (en) * 2018-06-13 2021-08-05 The Children's Medical Center Corporation Methods and compositions relating to high-throughput models for antibody discovery and/or optimization
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
JP7657726B2 (ja) 2019-03-19 2025-04-07 ザ ブロード インスティテュート，インコーポレーテッド 編集ヌクレオチド配列を編集するための方法および組成物
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
DE112021002672T5 (de) 2020-05-08 2023-04-13 President And Fellows Of Harvard College Vefahren und zusammensetzungen zum gleichzeitigen editieren beider stränge einer doppelsträngigen nukleotid-zielsequenz
WO2022256739A2 (en) * 2021-06-04 2022-12-08 Nonagen Therapeutics Corporation Antibody specific for bcl-6 and methods of use

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6130364A (en) 1995-03-29 2000-10-10 Abgenix, Inc. Production of antibodies using Cre-mediated site-specific recombination
ES2436044T3 (es) * 2008-05-23 2013-12-26 Ablexis, Llc Procedimiento de generación de anticuerpos de dominio VL individual en animales transgénicos
JP5827127B2 (ja) 2008-12-18 2015-12-02 エラスムス・ユニヴァーシティ・メディカル・センター・ロッテルダム ヒト化抗体を発現する非ヒトトランスジェニック動物及びその使用
RS59728B1 (sr) * 2011-08-05 2020-02-28 Regeneron Pharma Humanizovani miševi univerzalnog lakog lanca
BR112014006394A2 (pt) 2011-09-19 2017-03-28 Kymab Ltd manipulação de diversidade genética de imunoglobulina e terapêuticos multi-anticorpos
KR20160098514A (ko) * 2011-10-17 2016-08-18 리제너론 파아마슈티컬스, 인크. 제한된 면역글로불린 중쇄 마우스
PT3597037T (pt) * 2012-06-12 2021-06-01 Regeneron Pharma Animais não humanos humanizados com lócus de cadeia pesada de imunoglobulina restrito
US10034463B2 (en) * 2014-01-24 2018-07-31 Children's Medical Center Corporation High-throughput mouse model for optimizing antibody affinities

Similar Documents

Publication Publication Date Title
JP2017505127A5 (https=)
JP6876853B2 (ja) 動物モデルおよび治療用分子
US11224207B2 (en) Non-human animals expressing pH-sensitive immunoglobulin sequences
CN103228130B (zh) 动物模型及治疗分子
JP2022090103A (ja) 限定された免疫グロブリン重鎖遺伝子座を有するヒト化非ヒト動物
EP2509409B1 (en) Mice that make heavy chain antibodies
CN105861548B (zh) Adam6小鼠
JP2019068857A5 (https=)
JP2019022518A5 (https=)
JP2020124228A (ja) ヒト可変領域を保有している抗体を産生するために有用な繁殖可能遺伝子導入動物
JP2015510769A5 (https=)
CN111031789A (zh) 动物模型和治疗性分子
EP3046412A1 (en) Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same
RU2016123708A (ru) Отличные от человека животные, содержащие гуманизированный ген фактора активации в-клеток
EP2825036A1 (en) Histidine engineered light chain antibodies and genetically modified non-human animals for generating the same
WO2015077072A4 (en) Non-human animals having a humanized a proliferation-inducing ligand gene
JP2018525014A (ja) 共通の軽鎖を有する抗体を産生するためのトランスジェニック動物
JP2019506897A5 (https=)
JP2022538885A (ja) トランスジェニック哺乳動物およびその使用法
JP2021166551A (ja) 鳥類および卵
JP2021508236A5 (https=)
JP7765403B2 (ja) ヒト化ｃｘｃｌ１３遺伝子を有する非ヒト動物
WO2017175745A1 (ja) 生殖細胞欠損動物を用いる遺伝子改変動物の作製方法
JP2026508038A (ja) ヒト化抗体を産生する動物モデル及びその構築方法
HK40013873A (en) Non-human animals expressing ph-sensitive immunoglobulin sequences