JP2017504311A - 網膜色素上皮細胞を生成する方法 - Google Patents

網膜色素上皮細胞を生成する方法 Download PDF

Info

Publication number
JP2017504311A
JP2017504311A JP2016538000A JP2016538000A JP2017504311A JP 2017504311 A JP2017504311 A JP 2017504311A JP 2016538000 A JP2016538000 A JP 2016538000A JP 2016538000 A JP2016538000 A JP 2016538000A JP 2017504311 A JP2017504311 A JP 2017504311A
Authority
JP
Japan
Prior art keywords
cells
rpe
cultured
days
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
JP2016538000A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017504311A5 (enExample
Inventor
チャウダリー パールー
チャウダリー パールー
サーマツ−コードレ ベアタ
サーマツ−コードレ ベアタ
ドーン エレン ブース ヘザー
ドーン エレン ブース ヘザー
ジョン ホワイティング ポール
ジョン ホワイティング ポール
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pfizer Ltd
Original Assignee
Pfizer Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pfizer Ltd filed Critical Pfizer Ltd
Publication of JP2017504311A publication Critical patent/JP2017504311A/ja
Publication of JP2017504311A5 publication Critical patent/JP2017504311A5/ja
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/30—Nerves; Brain; Eyes; Corneal cells; Cerebrospinal fluid; Neuronal stem cells; Neuronal precursor cells; Glial cells; Oligodendrocytes; Schwann cells; Astroglia; Astrocytes; Choroid plexus; Spinal cord tissue
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P27/00—Drugs for disorders of the senses
    • A61P27/02—Ophthalmic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/01—Modulators of cAMP or cGMP, e.g. non-hydrolysable analogs, phosphodiesterase inhibitors, cholera toxin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Ophthalmology & Optometry (AREA)
  • General Health & Medical Sciences (AREA)
  • Neurosurgery (AREA)
  • Neurology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
JP2016538000A 2013-12-11 2014-12-08 網膜色素上皮細胞を生成する方法 Pending JP2017504311A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361914445P 2013-12-11 2013-12-11
US61/914,445 2013-12-11
PCT/IB2014/066703 WO2015087231A1 (en) 2013-12-11 2014-12-08 Method for producing retinal pigment epithelial cells

Publications (2)

Publication Number Publication Date
JP2017504311A true JP2017504311A (ja) 2017-02-09
JP2017504311A5 JP2017504311A5 (enExample) 2018-01-25

Family

ID=52446403

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2016538000A Pending JP2017504311A (ja) 2013-12-11 2014-12-08 網膜色素上皮細胞を生成する方法

Country Status (14)

Country Link
US (1) US20150159134A1 (enExample)
EP (1) EP3080248A1 (enExample)
JP (1) JP2017504311A (enExample)
KR (1) KR101871084B1 (enExample)
CN (1) CN105814194A (enExample)
AR (1) AR098693A1 (enExample)
AU (1) AU2014363032A1 (enExample)
BR (1) BR112016012129A8 (enExample)
CA (1) CA2933083A1 (enExample)
HK (1) HK1221734A1 (enExample)
IL (1) IL245594A0 (enExample)
MX (1) MX2016007671A (enExample)
TW (2) TW201538726A (enExample)
WO (1) WO2015087231A1 (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018501209A (ja) * 2014-11-21 2018-01-18 ライジェル ファーマシューティカルズ， インコーポレイテッド Ｔｇｆ−ベータ阻害剤としての縮合イミダゾール誘導体
JPWO2021014515A1 (enExample) * 2019-07-19 2021-01-28
JP2022532411A (ja) * 2019-05-15 2022-07-14 ノヴォ ノルディスク アー／エス ヒト多能性幹細胞から眼野前駆細胞を得るための方法

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
US20180011092A1 (en) * 2014-12-30 2018-01-11 Cell Cure Neurosciences Ltd. Assessing retinal pigment epithelial cell populations
CA2972700A1 (en) * 2014-12-30 2016-07-07 Cell Cure Neurosciences Ltd. Methods of treating retinal diseases
AU2015373050B2 (en) * 2014-12-30 2022-09-29 Cell Cure Neurosciences Ltd. RPE cell populations and methods of generating same
CN108290855A (zh) 2015-07-01 2018-07-17 西北大学 被取代的喹唑啉化合物及其调节葡糖脑苷脂酶活性的用途
CN108138144A (zh) * 2015-08-05 2018-06-08 细胞治疗神经科学有限公司 视网膜色素上皮细胞的制备
CN114807035B (zh) 2015-09-08 2024-02-02 (由卫生与公众服务部部长代表的)美利坚合众国 临床级别视网膜色素上皮细胞的可再现的分化方法
US11162070B2 (en) * 2015-09-08 2021-11-02 FUJIFILM Cellular Dynamics, Inc. MACS-based purification of stem cell-derived retinal pigment epithelium
AU2016320991C1 (en) * 2015-09-08 2023-04-06 Healios K.K. Method for producing retinal pigment epithelial cells
WO2017070333A1 (en) 2015-10-20 2017-04-27 Cellular Dynamics International, Inc. Multi-lineage hematopoietic precursor cell production by genetic programming
EP3384008A4 (en) 2015-12-04 2019-10-02 Bond University Ltd Methods of differentiating retinal cells
CN106344932B (zh) * 2016-08-26 2019-12-27 首都医科大学附属北京同仁医院 一种用于提高视网膜退行性疾病基因治疗效果的组合物及其应用
MX2019006576A (es) 2016-12-07 2019-08-21 Mayo Found Medical Education & Res Metodos y materiales para usar soportes de fibrina para trasplante de epitelio de pigmento retiniano.
WO2018146679A2 (en) * 2017-02-08 2018-08-16 Hadasit Medical Research Services And Development Ltd. Photoreceptor cells for the treatment of retinal diseases
US11618883B2 (en) 2017-03-08 2023-04-04 Sumitomo Pharma Co., Ltd. Method for producing retinal pigment epithelial cells
CN106906178A (zh) * 2017-04-07 2017-06-30 温州医科大学附属第二医院、温州医科大学附属育英儿童医院 培养上皮细胞的组合物及其保持上皮样细胞形态中的应用
CN110891967B (zh) 2017-04-18 2025-03-14 富士胶片细胞动力公司 抗原特异性免疫效应细胞
AU2018282179B2 (en) * 2017-06-05 2024-03-07 Mayo Foundation For Medical Education And Research Methods and materials for culturing, proliferating, and differentiating stem cells
KR20200057051A (ko) * 2017-09-27 2020-05-25 시질론 테라퓨틱스, 인크. 활성 세포를 포함하는 방법, 조성물 및 이식 가능한 요소
IL263217B (en) 2017-11-24 2022-06-01 Juul Labs Inc Puff sensing and power circuitry for vaporizer devices
CN109957545A (zh) 2017-12-26 2019-07-02 江苏艾尔康生物医药科技有限公司 一种消化rpe细胞的方法
BR112020013238A2 (pt) 2017-12-29 2020-12-01 Cell Cure Neurosciences Ltd. composição e método para formular células do epitélio pigmentar da retina bem como uso da dita composição para tratar uma condição retiniana
TW202012390A (zh) * 2018-06-29 2020-04-01 日商日本歐愛特農業科技股份有限公司 新穎之經取代吡唑衍生物
EP4008350A4 (en) 2019-07-26 2023-06-14 Saitama Medical University ANTIBODY RECOGNIZING THE EXTRACELLULAR REGION OF ALK2/ACVR1
CN113699097B (zh) * 2021-09-08 2023-06-09 湖南光琇高新生命科技有限公司 视网膜色素上皮细胞的制备方法
CN120905148B (zh) * 2025-10-11 2026-01-06 中国人民解放军陆军军医大学第一附属医院 一种基于ChR2和GCaMP5G双转染hESC构建起搏细胞制备视网膜类器官的方法

Family Cites Families (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG49267A1 (en) 1989-08-14 1998-05-18 Photogenesis Inc Surgical instrument and cell isolation and transplantation
US6045791A (en) 1992-03-06 2000-04-04 Photogenesis, Inc. Retinal pigment epithelium transplantation
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5941250A (en) 1996-11-21 1999-08-24 University Of Louisville Research Foundation Inc. Retinal tissue implantation method
CN1720055A (zh) 2002-10-04 2006-01-11 组织技术公司 视网膜上皮细胞在羊膜上的培养和移植
CA2937099A1 (en) 2004-01-23 2005-08-04 Astellas Institute For Regenerative Medicine Improved modalities for the treatment of degenerative diseases of the retina
US7893315B2 (en) 2004-11-04 2011-02-22 Advanced Cell Technology, Inc. Derivation of embryonic stem cells and embryo-derived cells
EP1812554A4 (en) 2004-11-04 2008-10-08 Advanced Cell Tech Inc DERIVATION OF EMBRYONAL STEM CELLS
EP2206724A1 (en) 2005-12-13 2010-07-14 Kyoto University Nuclear reprogramming factor
US20090227032A1 (en) 2005-12-13 2009-09-10 Kyoto University Nuclear reprogramming factor and induced pluripotent stem cells
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
EP2128244A4 (en) 2007-01-18 2012-05-09 Riken PROCESS FOR INDUCTION / DIFFERENTIATION TO A PHOTOREZEPTORZELLE
DK3399025T3 (da) 2007-03-23 2025-06-16 Wisconsin Alumni Res Found Omprogrammering af somatiske celler
CA2684460C (en) * 2007-04-18 2017-01-03 Hadasit Medical Research Services & Development Limited Stem cell-derived retinal pigment epithelial cells
JP2008307007A (ja) 2007-06-15 2008-12-25 Bayer Schering Pharma Ag 出生後のヒト組織由来未分化幹細胞から誘導したヒト多能性幹細胞
EP2209888B1 (en) 2007-10-12 2019-12-04 Astellas Institute for Regenerative Medicine Improved methods of producing rpe cells and compositions of rpe cells
GB0806746D0 (en) 2008-04-14 2008-05-14 Ucl Business Plc Membrane
EP3553170B1 (en) 2009-08-24 2024-02-14 Wisconsin Alumni Research Foundation Substantially pure human retinal progenitor, forebrain progenitor, and retinal pigment epithelium cell cultures and methods of making the same
IL281453B (en) 2009-11-17 2022-07-01 Astellas Inst For Regenerative Medicine Methods for preparing human rpe cells and pharmaceutical preparations of human rpe cells
ES2542226T3 (es) 2010-04-28 2015-08-03 Technische Universität Dresden Método para producir células progenitoras retinianas polarizadas a partir de células madre pluripotentes y su diferenciación para dar células del epitelio pigmentario retiniano
ES2747803T3 (es) * 2010-05-25 2020-03-11 Memorial Sloan Kettering Cancer Center Método de diferenciación a nociceptores de células madre embrionarias humanas y sus usos
GB201011313D0 (en) 2010-07-05 2010-08-18 Ucl Business Plc Implantation devices, methods and implants
AU2011279250B2 (en) 2010-07-12 2014-12-04 California Institute Of Technology Biocompatible substrate for facilitating interconnections between stem cells and target tissues and methods for implanting same
US8871744B2 (en) 2010-07-21 2014-10-28 B & G Partyers, LLC Compounds and methods for selectively targeting tumor-associated mucins
CN102465111A (zh) * 2010-11-19 2012-05-23 薛志刚 一种体外人胚胎干细胞诱导分化为视网膜色素上皮细胞的试验方法
WO2012099873A1 (en) 2011-01-18 2012-07-26 Minipums, Llc Surgical implantation instrument
WO2012177968A1 (en) 2011-06-22 2012-12-27 The Schepens Eye Research Institute, Inc. A scaffold for subretinal cell transplantation and drug delivery
IL287381B2 (en) 2011-11-14 2024-04-01 Astellas Inst For Regenerative Medicine Pharmaceutical preparations of human rpe cells and uses thereof
US9850463B2 (en) 2012-02-01 2017-12-26 The Regents Of The University Of California Methods of culturing retinal pigmented epithelium cells, including xeno-free production, RPE enrichment, and cryopreservation
CN102618488A (zh) * 2012-03-15 2012-08-01 中国人民解放军第三军医大学第一附属医院 一种制备视网膜色素上皮细胞的方法
WO2013184809A1 (en) 2012-06-05 2013-12-12 The Regents Of The University Of California Methods and compositions for the rapid production of retinal pigmented epithelial cells from pluripotent cells
DK2951290T3 (en) 2013-02-01 2018-01-22 Us Health PROCEDURE FOR PREPARING RETINAL PIGMENTAL EPIT (RPE) CELLS FROM INDUCED PLURIPOTENT STEM CELLS (IPSCS)

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CARR, A.J.F., ET AL.: "Development of human embryonic stem cell therapies for age-related macular degeneration", TRENDS IN NEUROSCIENCES, vol. 36, no. 7, JPN6018041744, 2013, pages 385 - 395, XP055173782, ISSN: 0003904502, DOI: 10.1016/j.tins.2013.03.006 *
IDELSON, M., ET AL.: "Directed differentiation of human embryonic stem cells into functional retinal pigment epithelium ce", CELL STEM CELL, vol. 5, no. 4, JPN6018041750, 2009, pages 396 - 408, XP055046648, ISSN: 0003904504, DOI: 10.1016/j.stem.2009.07.002 *
OSAKADA, F., ET AL.: "In vitro differentiation of retinal cells from human pluripotent stem cells by small-molecule induct", JOURNAL OF CELL SCIENCE, vol. 122, no. 17, JPN6018041747, 2009, pages 3169 - 3179, XP002604161, ISSN: 0003904503, DOI: 10.1242/JCS.050393 *

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018501209A (ja) * 2014-11-21 2018-01-18 ライジェル ファーマシューティカルズ， インコーポレイテッド Ｔｇｆ−ベータ阻害剤としての縮合イミダゾール誘導体
JP2022532411A (ja) * 2019-05-15 2022-07-14 ノヴォ ノルディスク アー／エス ヒト多能性幹細胞から眼野前駆細胞を得るための方法
JPWO2021014515A1 (enExample) * 2019-07-19 2021-01-28
WO2021014515A1 (ja) * 2019-07-19 2021-01-28 東京エレクトロン株式会社 細胞の分化状態の評価方法
JP7344421B2 (ja) 2019-07-19 2023-09-14 東京エレクトロン株式会社 細胞の分化状態の評価方法

Also Published As

Publication number Publication date
EP3080248A1 (en) 2016-10-19
BR112016012129A8 (pt) 2020-05-12
US20150159134A1 (en) 2015-06-11
CN105814194A (zh) 2016-07-27
HK1221734A1 (zh) 2017-06-09
AR098693A1 (es) 2016-06-08
TW201538726A (zh) 2015-10-16
KR20160095118A (ko) 2016-08-10
BR112016012129A2 (pt) 2017-08-08
CA2933083A1 (en) 2015-06-18
MX2016007671A (es) 2016-10-14
IL245594A0 (en) 2016-06-30
KR101871084B1 (ko) 2018-06-25
TW201823451A (zh) 2018-07-01
WO2015087231A1 (en) 2015-06-18
AU2014363032A1 (en) 2016-06-09

Similar Documents

Publication Publication Date Title
KR101871084B1 (ko) 망막 색소 상피 세포의 제조 방법
US11560546B2 (en) Methods for neural conversion of human embryonic stem cells
JP6995314B2 (ja) 網膜組織の製造法
JP2022123137A (ja) ヒト多能性幹細胞からの機能的頭蓋プラコード派生体の特定
JP5721111B2 (ja) 幹細胞の培地及び培養方法
JP5631210B2 (ja) 多能性幹細胞からのニューロン細胞の生成
JP7029144B2 (ja) 腺性下垂体又はその前駆組織の製造方法
WO2016063985A1 (ja) 神経組織の製造方法
AU2017214749B2 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
JP2014082956A (ja) 細胞培養基材、およびそれを用いた細胞培養方法並びに多能性幹細胞の分化誘導方法
JP7766724B2 (ja) オリゴデンドロサイト前駆細胞組成物
US20200199530A1 (en) Methods of differentiating stem cell-derived ectodermal lineage precursors
Klimanskaya Retinal pigment epithelium
JP7737672B2 (ja) 下垂体ホルモン産生細胞及びその前駆細胞の分離法
JP2022538503A (ja) ヒト多能性幹細胞由来の神経幹細胞株の生成
Rattier et al. Disruption of epithelial integrity drives mesendoderm differentiation in human induced pluripotent stem cells by enabling BMP and ACTIVIN sensing
WO2024242069A1 (ja) 神経堤細胞の製造方法
US20220259558A1 (en) Methods for obtaining eye field progenitor cells from human pluripotent stem cells
WO2023054325A1 (ja) 細胞表面マーカーを用いた下垂体細胞および視床下部細胞の分離法
EP4689063A1 (en) Compositions and methods for differentiating rpe cells
HK40086565A (en) Method for separating pituitary hormone-producing cells and progenitor cells thereof

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20171206

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20171206

A977 Report on retrieval

Free format text: JAPANESE INTERMEDIATE CODE: A971007

Effective date: 20180926

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20181030

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20190122

RD03 Notification of appointment of power of attorney

Free format text: JAPANESE INTERMEDIATE CODE: A7423

Effective date: 20190122

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20190625