JP2017189179A5 - - Google Patents
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- JP2017189179A5 JP2017189179A5 JP2017146473A JP2017146473A JP2017189179A5 JP 2017189179 A5 JP2017189179 A5 JP 2017189179A5 JP 2017146473 A JP2017146473 A JP 2017146473A JP 2017146473 A JP2017146473 A JP 2017146473A JP 2017189179 A5 JP2017189179 A5 JP 2017189179A5
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- Prior art keywords
- cell
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- virus
- bacterial
- bacterial dna
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- 210000004027 cells Anatomy 0.000 claims 21
- 108020000946 Bacterial DNA Proteins 0.000 claims 8
- 241000700605 Viruses Species 0.000 claims 6
- 230000001580 bacterial Effects 0.000 claims 6
- 241000712461 unidentified influenza virus Species 0.000 claims 6
- 210000004962 mammalian cells Anatomy 0.000 claims 4
- 241000271566 Aves Species 0.000 claims 3
- 229920001405 Coding region Polymers 0.000 claims 3
- 238000004519 manufacturing process Methods 0.000 claims 3
- 241000894006 Bacteria Species 0.000 claims 2
- 239000003550 marker Substances 0.000 claims 2
- 230000035897 transcription Effects 0.000 claims 2
- 229960005486 vaccines Drugs 0.000 claims 2
- 241000712431 Influenza A virus Species 0.000 claims 1
- 241000701076 Macacine alphaherpesvirus 1 Species 0.000 claims 1
- 210000003501 Vero Cells Anatomy 0.000 claims 1
- 229920003013 deoxyribonucleic acid Polymers 0.000 claims 1
- 210000003292 kidney cell Anatomy 0.000 claims 1
- 230000000644 propagated Effects 0.000 claims 1
- 230000001902 propagating Effects 0.000 claims 1
- 230000002194 synthesizing Effects 0.000 claims 1
Claims (15)
- プロモーターと、インフルエンザウイルスのＰＢ２、ＰＢ１、ＰＡ、ＮＰ及びＮＳセグメントを発現するためのコード配列とを含む非細菌性ＤＮＡ構築物であって、該構築物は、細菌複製起点と細菌選択マーカーの両方を欠き、線状であり、かつ、該プロモーターは、真核宿主細胞における転写のために作動可能に連結されており、該真核宿主細胞は、ほ乳動物細胞又はトリ細胞である、非細菌性ＤＮＡ構築物。
- プロモーターと、Ａ型又はＢ型インフルエンザウイルスの全８種のゲノムセグメントを発現するコード配列とを含む、請求項１に記載の非細菌性ＤＮＡ構築物。
- （ｉ）複数の断片を化学合成する工程であって、各断片の端が隣接する５’又は３’断片と重複しており、該複数の断片が前記非細菌性ＤＮＡ構築物に広がっている工程、及び（ｉｉ）該断片を連結して該非細菌性ＤＮＡ構築物を提供する工程、を含む方法によって調製された、請求項１又は請求項２に記載の非細菌性ＤＮＡ構築物であって、該方法において細菌が必要とされない、非細菌性ＤＮＡ構築物。
- 請求項１から３のいずれか一項に記載の非細菌性ＤＮＡ構築物を含む、組成物。
- 請求項１から３のいずれか一項に記載の非細菌性ＤＮＡ構築物を含む真核宿主細胞を調製するための方法であって、請求項１から３のいずれか一項に記載の構築物を該細胞に挿入する工程を含む、方法。
- インフルエンザウイルスを生成するための方法であって、該インフルエンザウイルスセグメントの発現が起きて該ウイルスを生成するように、請求項５に記載の宿主細胞を培養する工程を含む、方法。
- （ｉ）請求項１に記載の第１の非細菌性発現構築物、及び（ｉｉ）プロモーターと、Ａ型又はＢ型インフルエンザウイルスゲノムセグメントＨＡを発現するためのコード配列とを含む第２の非細菌性発現構築物、を含む発現構築物のセットであって、該構築物は、細菌複製起点と細菌選択マーカーの両方を欠き、線状であり、かつ、該プロモーターは、真核宿主細胞における転写のために作動可能に連結されており、該真核宿主細胞は、ほ乳動物細胞又はトリ細胞である、セット。
- 前記第１又は第２の非細菌性発現構築物がさらに、ＮＡゲノムセグメント及びＭゲノムセグメントを含む、請求項７に記載のセット。
- 請求項７又は請求項８に記載の構築物のセットを含む、真核宿主細胞。
- 前記細胞が、ほ乳動物細胞又はトリ細胞である、請求項９に記載の宿主細胞。
- 前記ほ乳動物細胞が腎臓細胞である、請求項１０に記載の宿主細胞。
- 前記細胞がＭＤＣＫ細胞、ＰＥＲ．Ｃ６細胞又はＶｅｒｏ細胞である、請求項９に記載の宿主細胞。
- 請求項１０から１２のいずれか一項に記載の宿主細胞を調製するための方法であって、請求項７又は請求項８に記載の発現構築物のセットを該細胞に挿入する工程を含む、方法。
- インフルエンザウイルスを生成するための方法であって、該インフルエンザウイルスセグメントの発現が起きて該ウイルスを生成するように、請求項１０から１２のいずれか一項に記載の宿主細胞を培養する工程を含む、方法。
- ワクチンを製造するための方法であって、（ａ）請求項１４に記載の方法によってウイルスを生成する工程；（ｂ）培養宿主を該ウイルスに感染させる工程；（ｃ）該ウイルスを増殖させる工程；及び（ｄ）該増殖させたウイルスからワクチンを製造する工程、を含む、方法。
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US27315109P | 2009-07-31 | 2009-07-31 | |
| US61/273,151 | 2009-07-31 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012522275A Division JP2013500712A (ja) | 2009-07-31 | 2010-07-30 | 逆遺伝学系 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2017189179A JP2017189179A (ja) | 2017-10-19 |
| JP2017189179A5 true JP2017189179A5 (ja) | 2018-02-01 |
| JP6666311B2 JP6666311B2 (ja) | 2020-03-13 |
Family
ID=43018886
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012522275A Withdrawn JP2013500712A (ja) | 2009-07-31 | 2010-07-30 | 逆遺伝学系 |
| JP2015215655A Pending JP2016019547A (ja) | 2009-07-31 | 2015-11-02 | 逆遺伝学系 |
| JP2017146473A Active JP6666311B2 (ja) | 2009-07-31 | 2017-07-28 | 逆遺伝学系 |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2012522275A Withdrawn JP2013500712A (ja) | 2009-07-31 | 2010-07-30 | 逆遺伝学系 |
| JP2015215655A Pending JP2016019547A (ja) | 2009-07-31 | 2015-11-02 | 逆遺伝学系 |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US9821052B2 (ja) |
| EP (1) | EP2459722B1 (ja) |
| JP (3) | JP2013500712A (ja) |
| KR (1) | KR20120039047A (ja) |
| CN (2) | CN104862335A (ja) |
| AU (1) | AU2010277310B2 (ja) |
| CA (1) | CA2769673A1 (ja) |
| HK (1) | HK1175814A1 (ja) |
| WO (1) | WO2011012999A1 (ja) |
Families Citing this family (17)
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| KR101323582B1 (ko) * | 2011-03-17 | 2013-10-30 | 충북대학교 산학협력단 | Ｖｅｒｏ 세포 유래의 ｐｏｌⅠ 프로모터 및 이를 포함하는 재조합 벡터 |
| WO2013101783A2 (en) | 2011-12-30 | 2013-07-04 | Bio-Rad Laboratories, Inc. | Methods and compositions for performing nucleic acid amplification reactions |
| WO2013087945A2 (en) | 2012-03-02 | 2013-06-20 | Novartis Ag | Influenza virus reassortment |
| WO2014086732A2 (en) | 2012-12-03 | 2014-06-12 | Novartis Ag | Influenza virus reassortment |
| US20140274806A1 (en) | 2013-03-15 | 2014-09-18 | Synthetic Genomics Vaccines | Influenza virus reassortment |
| WO2014115104A1 (en) * | 2013-01-23 | 2014-07-31 | Novartis Ag | Influenza virus reassortment |
| MX2015011529A (es) | 2013-03-13 | 2016-02-05 | Novartis Ag | Reordenamiento del virus de infuenza b. |
| WO2014145261A2 (en) * | 2013-03-15 | 2014-09-18 | University Of Maryland | Methods and compositions for in vivo immune stimulation and antigen production |
| DE202013005130U1 (de) | 2013-06-05 | 2013-09-10 | Novartis Ag | Influenza Virus Reassortierung |
| DE202013005100U1 (de) | 2013-06-05 | 2013-08-26 | Novartis Ag | Influenza Virus Reassortierung |
| WO2014195920A2 (en) | 2013-06-06 | 2014-12-11 | Novartis Ag | Influenza virus reassortment |
| WO2016157208A1 (en) * | 2015-03-27 | 2016-10-06 | Cadila Healthcare Limited | Recombinant mumps virus jeryl lynn 2 based vaccine |
| US10726110B2 (en) * | 2017-03-01 | 2020-07-28 | Seven Bridges Genomics, Inc. | Watermarking for data security in bioinformatic sequence analysis |
| CN114040979B (zh) * | 2019-06-21 | 2024-06-28 | 国立大学法人大阪大学 | 稳定地保持外源基因的人工重组rna病毒的制作方法 |
| CN110305854B (zh) * | 2019-07-18 | 2023-09-15 | 山东中医药大学 | 一种携带荧光素酶的重组h3n2亚型流感病毒、构建方法及应用 |
| JP2023502650A (ja) | 2019-11-18 | 2023-01-25 | セキラス ピーティーワイ リミテッド | 遺伝子再集合インフルエンザウイルスを産生するための方法 |
| CN114395568A (zh) * | 2021-10-29 | 2022-04-26 | 扬州大学 | 一种猪流行性腹泻病毒感染性cDNA克隆及其构建方法与应用 |
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| WO1995011700A1 (en) | 1993-10-29 | 1995-05-04 | Pharmos Corp. | Submicron emulsions as vaccine adjuvants |
| DE19612966B4 (de) | 1996-04-01 | 2009-12-10 | Novartis Vaccines And Diagnostics Gmbh & Co. Kg | MDCK-Zellen und Verfahren zur Vermehrung von Influenzaviren |
| DE19612967A1 (de) | 1996-04-01 | 1997-10-02 | Behringwerke Ag | Verfahren zur Vermehrung von Influenzaviren in Zellkultur, sowie die durch das Verfahren erhältlichen Influenzaviren |
| TW570803B (en) | 1997-04-09 | 2004-01-11 | Duphar Int Res | Influenza vaccine |
| WO1999050419A2 (en) | 1998-03-31 | 1999-10-07 | University Of Maryland Biotechnology Institute | A method for generating nonpathogenic, infectious pancreatic necrosis virus (ipnv) from synthetic rna transcripts |
| ES2400445T3 (es) | 1998-06-12 | 2013-04-09 | Mount Sinai School Of Medicine | Virus atenuados con cadena de polaridad negativa con actividad antagonista de interferón alterada para uso como vacunas y productos farmacéuticos |
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-
2010
- 2010-07-30 EP EP10754367.0A patent/EP2459722B1/en active Active
- 2010-07-30 CA CA2769673A patent/CA2769673A1/en not_active Abandoned
- 2010-07-30 CN CN201510264548.1A patent/CN104862335A/zh active Pending
- 2010-07-30 WO PCT/IB2010/002137 patent/WO2011012999A1/en active Application Filing
- 2010-07-30 CN CN201080041918.0A patent/CN102666860B/zh active Active
- 2010-07-30 AU AU2010277310A patent/AU2010277310B2/en active Active
- 2010-07-30 US US13/388,033 patent/US9821052B2/en active Active
- 2010-07-30 KR KR1020127005113A patent/KR20120039047A/ko not_active Application Discontinuation
- 2010-07-30 JP JP2012522275A patent/JP2013500712A/ja not_active Withdrawn
-
2013
- 2013-03-12 HK HK13103109.7A patent/HK1175814A1/xx unknown
-
2015
- 2015-11-02 JP JP2015215655A patent/JP2016019547A/ja active Pending
-
2017
- 2017-07-28 JP JP2017146473A patent/JP6666311B2/ja active Active
- 2017-10-16 US US15/784,575 patent/US10610584B2/en active Active
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