JP2017108748A5 - - Google Patents

Download PDF

Info

Publication number
JP2017108748A5
JP2017108748A5 JP2017018800A JP2017018800A JP2017108748A5 JP 2017108748 A5 JP2017108748 A5 JP 2017108748A5 JP 2017018800 A JP2017018800 A JP 2017018800A JP 2017018800 A JP2017018800 A JP 2017018800A JP 2017108748 A5 JP2017108748 A5 JP 2017108748A5
Authority
JP
Japan
Prior art keywords
probe
tag
reverse
labeled
ligation product
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
JP2017018800A
Other languages
English (en)
Japanese (ja)
Other versions
JP2017108748A (ja
JP6397065B2 (ja
Filing date
Publication date
Application filed filed Critical
Publication of JP2017108748A publication Critical patent/JP2017108748A/ja
Publication of JP2017108748A5 publication Critical patent/JP2017108748A5/ja
Application granted granted Critical
Publication of JP6397065B2 publication Critical patent/JP6397065B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

JP2017018800A 2013-08-19 2017-02-03 単分子検出アッセイおよびその使用 Active JP6397065B2 (ja)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201361867559P 2013-08-19 2013-08-19
US201361867554P 2013-08-19 2013-08-19
US61/867,559 2013-08-19
US61/867,554 2013-08-19

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
JP2016536393A Division JP6088710B2 (ja) 2013-08-19 2014-08-19 単分子検出アッセイおよびその使用

Publications (3)

Publication Number Publication Date
JP2017108748A JP2017108748A (ja) 2017-06-22
JP2017108748A5 true JP2017108748A5 (enExample) 2018-02-08
JP6397065B2 JP6397065B2 (ja) 2018-09-26

Family

ID=52484110

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2016536393A Active JP6088710B2 (ja) 2013-08-19 2014-08-19 単分子検出アッセイおよびその使用
JP2017018800A Active JP6397065B2 (ja) 2013-08-19 2017-02-03 単分子検出アッセイおよびその使用

Family Applications Before (1)

Application Number Title Priority Date Filing Date
JP2016536393A Active JP6088710B2 (ja) 2013-08-19 2014-08-19 単分子検出アッセイおよびその使用

Country Status (12)

Country Link
US (5) US9212394B2 (enExample)
EP (3) EP3726213A1 (enExample)
JP (2) JP6088710B2 (enExample)
CN (2) CN105659091A (enExample)
AU (2) AU2014308980C1 (enExample)
BR (1) BR112016003480B1 (enExample)
ES (2) ES3057032T3 (enExample)
IL (3) IL285521B2 (enExample)
MX (2) MX371428B (enExample)
SG (1) SG11201601201VA (enExample)
WO (1) WO2015026873A1 (enExample)
ZA (1) ZA201601029B (enExample)

Families Citing this family (54)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11111543B2 (en) 2005-07-29 2021-09-07 Natera, Inc. System and method for cleaning noisy genetic data and determining chromosome copy number
US9424392B2 (en) 2005-11-26 2016-08-23 Natera, Inc. System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals
US9677118B2 (en) 2014-04-21 2017-06-13 Natera, Inc. Methods for simultaneous amplification of target loci
US12152275B2 (en) 2010-05-18 2024-11-26 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12655483B2 (en) 2010-05-18 2026-06-16 Natera, Inc. Methods for simultaneous amplification of target loci
US20190010543A1 (en) 2010-05-18 2019-01-10 Natera, Inc. Methods for simultaneous amplification of target loci
US10316362B2 (en) 2010-05-18 2019-06-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11322224B2 (en) 2010-05-18 2022-05-03 Natera, Inc. Methods for non-invasive prenatal ploidy calling
US12221653B2 (en) 2010-05-18 2025-02-11 Natera, Inc. Methods for simultaneous amplification of target loci
US11939634B2 (en) 2010-05-18 2024-03-26 Natera, Inc. Methods for simultaneous amplification of target loci
JP6153874B2 (ja) 2011-02-09 2017-06-28 ナテラ， インコーポレイテッド 非侵襲的出生前倍数性呼び出しのための方法
US10648030B2 (en) 2012-01-13 2020-05-12 Affymetrix, Inc. Methods of determining the presence or absence of a plurality of target polynucleotides in a sample
US9892230B2 (en) 2012-03-08 2018-02-13 The Chinese University Of Hong Kong Size-based analysis of fetal or tumor DNA fraction in plasma
US20140100126A1 (en) 2012-08-17 2014-04-10 Natera, Inc. Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data
CN105659091A (zh) * 2013-08-19 2016-06-08 卓异生物公司 用于单分子检测的测定及其应用
US12492429B2 (en) 2014-04-21 2025-12-09 Natera, Inc. Detecting mutations and ploidy in chromosomal segments
RU2717641C2 (ru) 2014-04-21 2020-03-24 Натера, Инк. Обнаружение мутаций и плоидности в хромосомных сегментах
US20180173845A1 (en) 2014-06-05 2018-06-21 Natera, Inc. Systems and Methods for Detection of Aneuploidy
TWI758011B (zh) 2014-07-18 2022-03-11 香港中文大學 Ｄｎａ混合物中之組織甲基化模式分析
US10364467B2 (en) 2015-01-13 2019-07-30 The Chinese University Of Hong Kong Using size and number aberrations in plasma DNA for detecting cancer
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof
AU2016229372B2 (en) * 2015-03-06 2020-01-30 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
US10221448B2 (en) 2015-03-06 2019-03-05 Pillar Biosciences Inc. Selective amplification of overlapping amplicons
EP4428863A3 (en) 2015-05-11 2024-12-11 Natera, Inc. Methods and compositions for determining ploidy
WO2017007586A1 (en) 2015-07-07 2017-01-12 Pillar Biosciences Inc. Method for reducing primer-dimer amplification
US11118216B2 (en) * 2015-09-08 2021-09-14 Affymetrix, Inc. Nucleic acid analysis by joining barcoded polynucleotide probes
JP7280044B2 (ja) 2016-04-15 2023-05-23 ナテラ， インコーポレイテッド 肺癌の検出方法
CN106053405B (zh) * 2016-05-10 2018-10-02 东南大学 一种基于单分子定位法的超分辨光学成像方法
WO2017205827A1 (en) * 2016-05-26 2017-11-30 Singular Bio, Inc. Arrays for single molecule detection and uses thereof
WO2018045270A1 (en) 2016-09-02 2018-03-08 Singular Bio, Inc. Systems and methods for single molecule quantification
GB201618485D0 (en) 2016-11-02 2016-12-14 Ucl Business Plc Method of detecting tumour recurrence
IL266347B2 (en) 2016-11-30 2025-01-01 Univ Hong Kong Chinese Analysis of cell-free DNA in urine and other samples
US11093307B2 (en) * 2016-12-08 2021-08-17 Accenture Global Solutions Limited Platform for supporting multiple virtual agent applications
EP3595806A4 (en) * 2017-03-17 2021-06-16 Apton Biosystems, Inc. SEQUENCING AND HIGH RESOLUTION IMAGING
CN110945136A (zh) 2017-06-20 2020-03-31 威斯康星州立大学医学院 使用总无细胞dna评估移植并发症风险
WO2019118926A1 (en) 2017-12-14 2019-06-20 Tai Diagnostics, Inc. Assessing graft suitability for transplantation
EP3737773B1 (en) 2018-01-12 2023-04-12 Natera, Inc. Novel primers and uses thereof
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019200326A1 (en) 2018-04-13 2019-10-17 Rarecyte, Inc. Kits for labeling of biomarkers and methods of using the same
DK3781714T3 (da) 2018-04-14 2026-03-30 Natera Inc Fremgangsmåder til cancerpåvisning og -overvågning ved personlig påvisning af cirkulerende tumor-dna
US12234509B2 (en) 2018-07-03 2025-02-25 Natera, Inc. Methods for detection of donor-derived cell-free DNA
EP3591401A1 (de) * 2018-07-06 2020-01-08 Euroimmun Medizinische Labordiagnostika AG Verfahren zum automatisierten detektieren von antikörpern in einer flüssigen biologischen probe unter verwendung eines antigen-chips und antigen-chip hierfür
US11931674B2 (en) 2019-04-04 2024-03-19 Natera, Inc. Materials and methods for processing blood samples
JPWO2021117667A1 (enExample) * 2019-12-09 2021-06-17
US11211147B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing
US11211144B2 (en) 2020-02-18 2021-12-28 Tempus Labs, Inc. Methods and systems for refining copy number variation in a liquid biopsy assay
US11475981B2 (en) 2020-02-18 2022-10-18 Tempus Labs, Inc. Methods and systems for dynamic variant thresholding in a liquid biopsy assay
CN112345766B (zh) * 2020-10-22 2024-02-02 中国工程物理研究院核物理与化学研究所 一种荧光-放射性联用的体外靶向筛选方法
CN112419235B (zh) * 2020-10-22 2024-07-23 南京大学 一种精子活性检测系统及方法
AU2022227563A1 (en) 2021-02-23 2023-08-24 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
EP4092136B8 (en) * 2021-05-20 2024-03-13 Sophia Genetics S.A. Capture probes and uses thereof
EP4441277A4 (en) * 2021-12-01 2026-04-08 Siscapa Assay Tech Inc DETECTION OF ENRICHED PEPTIDES BY SINGLE-MOLECULE SEQUENCING
EP4581367A4 (en) * 2022-09-01 2026-07-15 Univ Johns Hopkins METHODS FOR MEASURING TELOMERE LENGTH
WO2025199499A1 (en) * 2024-03-22 2025-09-25 Orbit Genomics, Inc. Methods and systems for sample classification

Family Cites Families (100)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5256536A (en) 1990-11-09 1993-10-26 Syntex (U.S.A.) Inc. Nucleotide probe for Neisseria gonrrhoeae
US6582908B2 (en) 1990-12-06 2003-06-24 Affymetrix, Inc. Oligonucleotides
DK0562025T3 (da) 1990-12-06 2001-06-18 Affymetrix Inc Forbindelser og deres anvendelse i en binær syntesestrategi
EP0566670A4 (en) 1990-12-17 1993-12-08 Idexx Laboratories, Inc. Nucleic acid sequence detection by triple helix formation
EP1382386A3 (en) 1992-02-19 2004-12-01 The Public Health Research Institute Of The City Of New York, Inc. Novel oligonucleotide arrays and their use for sorting, isolating, sequencing, and manipulating nucleic acids
US5670330A (en) 1992-09-29 1997-09-23 Mcgill University Anti-tumor agent assay using PKR
US5681943A (en) 1993-04-12 1997-10-28 Northwestern University Method for covalently linking adjacent oligonucleotides
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
ATE496288T1 (de) 1995-10-11 2011-02-15 Luminex Corp Gleichzeitige mehrfachanalyse klinischer proben
AU735440B2 (en) * 1996-02-09 2001-07-05 Cornell Research Foundation Inc. Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
AU730633B2 (en) * 1996-05-29 2001-03-08 Phillip Belgrader Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
US6706473B1 (en) 1996-12-06 2004-03-16 Nanogen, Inc. Systems and devices for photoelectrophoretic transport and hybridization of oligonucleotides
US6028189A (en) 1997-03-20 2000-02-22 University Of Washington Solvent for oligonucleotide synthesis and methods of use
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
CA2243985C (en) 1997-09-11 2004-08-10 F. Hoffmann-La Roche Ag Thermostable dna polymerases incorporating nucleoside triphosphates labeled with fluorescein family dyes
US6465178B2 (en) 1997-09-30 2002-10-15 Surmodics, Inc. Target molecule attachment to surfaces
US6238869B1 (en) 1997-12-19 2001-05-29 High Throughput Genomics, Inc. High throughput assay system
US6268146B1 (en) 1998-03-13 2001-07-31 Promega Corporation Analytical methods and materials for nucleic acid detection
US6284497B1 (en) 1998-04-09 2001-09-04 Trustees Of Boston University Nucleic acid arrays and methods of synthesis
AU770831B2 (en) 1998-07-30 2004-03-04 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
US6432642B1 (en) 1999-01-15 2002-08-13 Pe Corporation (Ny) Binary probe and clamp composition and methods for a target hybridization detection
US6506594B1 (en) * 1999-03-19 2003-01-14 Cornell Res Foundation Inc Detection of nucleic acid sequence differences using the ligase detection reaction with addressable arrays
US6238868B1 (en) 1999-04-12 2001-05-29 Nanogen/Becton Dickinson Partnership Multiplex amplification and separation of nucleic acid sequences using ligation-dependant strand displacement amplification and bioelectronic chip technology
US20060275782A1 (en) 1999-04-20 2006-12-07 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US20020086289A1 (en) 1999-06-15 2002-07-04 Don Straus Genomic profiling: a rapid method for testing a complex biological sample for the presence of many types of organisms
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US6495320B1 (en) 1999-07-21 2002-12-17 Affymetrix, Inc. Even length proportional amplification of nucleic acids
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
JP2003521252A (ja) 2000-02-07 2003-07-15 イルミナ インコーポレイテッド ユニバーサルプライミングを用いる核酸検出方法
US6913884B2 (en) 2001-08-16 2005-07-05 Illumina, Inc. Compositions and methods for repetitive use of genomic DNA
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
CA2405412A1 (en) 2000-04-14 2001-10-25 Cornell Research Foundation, Inc. Method of designing addressable array for detection of nucleic acid sequence differences using ligase detection reaction
WO2001092579A2 (en) * 2000-05-30 2001-12-06 Pe Corporation (Ny) Methods for detecting target nucleic acids using coupled ligation and amplification
US6635425B2 (en) 2001-01-05 2003-10-21 Molecular Staging, Inc. 5′-thio phosphate directed ligation of oligonucleotides and use in detection of single nucleotide polymorphisms
EP2801624B1 (en) 2001-03-16 2019-03-06 Singular Bio, Inc Arrays and methods of use
US20020197630A1 (en) 2001-04-12 2002-12-26 Knapp Michael R. Systems and methods for high throughput genetic analysis
JP2006510348A (ja) 2002-07-18 2006-03-30 ザ・ボード・オブ・トラスティーズ・オブ・ザ・レランド・スタンフォード・ジュニア・ユニバーシティ 蛍光消光脱離基を用いる化学ライゲーションの検出
AU2003267583A1 (en) * 2002-09-19 2004-04-08 The Chancellor, Master And Scholars Of The University Of Oxford Molecular arrays and single molecule detection
US7153658B2 (en) 2002-09-19 2006-12-26 Applera Corporation Methods and compositions for detecting targets
CN1791682B (zh) 2003-02-26 2013-05-22 凯利达基因组股份有限公司 通过杂交进行的随机阵列dna分析
US7105024B2 (en) 2003-05-06 2006-09-12 Aesculap Ii, Inc. Artificial intervertebral disc
EP1602733A1 (en) 2004-06-02 2005-12-07 Keygene N.V. Detection of target nucleotide sequences using an asymmetric oligonucleotide ligation assay
WO2005026329A2 (en) * 2003-09-12 2005-03-24 Cornell Research Foundation, Inc. Methods for identifying target nucleic acid molecules
DE60328193D1 (de) 2003-10-16 2009-08-13 Sequenom Inc Nicht invasiver Nachweis fötaler genetischer Merkmale
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
CN101087890A (zh) * 2004-07-26 2007-12-12 帕拉列勒生物科学公司 多个基因组的同时分析
EP1859051A4 (en) * 2005-03-18 2009-08-05 Univ Hong Kong Chinese MARKERS FOR PRENATAL DIAGNOSIS AND SURVEILLANCE
CN1824786A (zh) * 2005-12-23 2006-08-30 上海生物芯片有限公司 基于连接的选择性扩增方法
US20080038733A1 (en) 2006-03-28 2008-02-14 Baylor College Of Medicine Screening for down syndrome
EP2029779A4 (en) * 2006-06-14 2010-01-20 Living Microsystems Inc HIGHLY PARALLEL SNP GENOTYPING UTILIZATION FOR FETAL DIAGNOSIS
US8137912B2 (en) * 2006-06-14 2012-03-20 The General Hospital Corporation Methods for the diagnosis of fetal abnormalities
JP5165933B2 (ja) * 2007-06-12 2013-03-21 日本碍子株式会社 標的核酸中の特定部分配列の検出方法及びアレイ
JP2010532665A (ja) 2007-07-11 2010-10-14 ユニヴェルシテ ラヴァル 細菌性および真菌性敗血症の病原菌の高感度な増幅および検出用の核酸配列およびその組合せ
US20100297585A1 (en) 2008-01-29 2010-11-25 Uriel Yarovesky Process for making a dental restoration and resultant apparatus
JP5319148B2 (ja) * 2008-03-27 2013-10-16 日本碍子株式会社 標的核酸中の変異の検出方法及びアレイ
US20120252015A1 (en) 2011-02-18 2012-10-04 Bio-Rad Laboratories Methods and compositions for detecting genetic material
AU2009311769A1 (en) 2008-11-05 2010-05-14 Stichting Sanquin Bloedvoorziening Means and methods for investigating nucleic acid sequences
US20100273164A1 (en) 2009-03-24 2010-10-28 President And Fellows Of Harvard College Targeted and Whole-Genome Technologies to Profile DNA Cytosine Methylation
WO2010148115A1 (en) 2009-06-18 2010-12-23 The Penn State Research Foundation Methods, systems and kits for detecting protein-nucleic acid interactions
US20120196765A1 (en) * 2009-07-09 2012-08-02 Mitsuo Kawase Method for detection or analysis of target sequence in genomic dna
CN101864482A (zh) * 2010-02-10 2010-10-20 深圳出入境检验检疫局动植物检验检疫技术中心 Mlcr探针、二步反应模式和悬浮芯片检测用捕获探针
WO2011142836A2 (en) * 2010-05-14 2011-11-17 Fluidigm Corporation Assays for the detection of genotype, mutations, and/or aneuploidy
JP2013528061A (ja) 2010-06-07 2013-07-08 エソテリックス ジェネティック ラボラトリーズ， エルエルシー 核酸の数え上げ
EP2596127A2 (en) * 2010-07-23 2013-05-29 Esoterix Genetic Laboratories, LLC Identification of differentially represented fetal or maternal genomic regions and uses thereof
US20140342940A1 (en) * 2011-01-25 2014-11-20 Ariosa Diagnostics, Inc. Detection of Target Nucleic Acids using Hybridization
US20120077185A1 (en) 2010-08-06 2012-03-29 Tandem Diagnostics, Inc. Detection of genetic abnormalities and infectious disease
US20130040375A1 (en) 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
US8700338B2 (en) 2011-01-25 2014-04-15 Ariosa Diagnosis, Inc. Risk calculation for evaluation of fetal aneuploidy
US10167508B2 (en) 2010-08-06 2019-01-01 Ariosa Diagnostics, Inc. Detection of genetic abnormalities
US20130261003A1 (en) 2010-08-06 2013-10-03 Ariosa Diagnostics, In. Ligation-based detection of genetic variants
US20120034603A1 (en) 2010-08-06 2012-02-09 Tandem Diagnostics, Inc. Ligation-based detection of genetic variants
US20120190557A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Risk calculation for evaluation of fetal aneuploidy
CN103228796B (zh) * 2010-08-11 2016-06-01 赛路拉公司 基因分型dna
US11270781B2 (en) 2011-01-25 2022-03-08 Ariosa Diagnostics, Inc. Statistical analysis for non-invasive sex chromosome aneuploidy determination
US9994897B2 (en) 2013-03-08 2018-06-12 Ariosa Diagnostics, Inc. Non-invasive fetal sex determination
US10131947B2 (en) 2011-01-25 2018-11-20 Ariosa Diagnostics, Inc. Noninvasive detection of fetal aneuploidy in egg donor pregnancies
US20120190021A1 (en) 2011-01-25 2012-07-26 Aria Diagnostics, Inc. Detection of genetic abnormalities
WO2012118745A1 (en) 2011-02-28 2012-09-07 Arnold Oliphant Assay systems for detection of aneuploidy and sex determination
US8759036B2 (en) * 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
WO2012135658A2 (en) 2011-03-30 2012-10-04 Noblegen Biosciences, Inc. Sequence preserved dna conversion for optical nanopore sequencing
AU2012281242B2 (en) 2011-07-08 2016-12-22 Keygene N.V. Sequence based genotyping based on oligonucleotide ligation assays
EP2764458B1 (en) 2011-10-06 2021-04-07 Sequenom, Inc. Methods and processes for non-invasive assessment of genetic variations
CN104204228A (zh) 2012-02-14 2014-12-10 康奈尔大学 使用组合的核酸酶、连接和聚合酶反应用于核酸序列的相对定量、表达或拷贝变化的方法
US20130310262A1 (en) 2012-05-21 2013-11-21 Ariosa Diagnostics, Inc. Noninvasive detection of robertsonian translocations
CN104583421A (zh) 2012-07-19 2015-04-29 阿瑞奥萨诊断公司 遗传变体的基于多重的顺序连接的检测
CN102936755B (zh) * 2012-10-19 2015-01-21 上海交通大学 一种单分子核酸芯片的制作方法
WO2014089536A1 (en) 2012-12-07 2014-06-12 Invitae Corporation Multiplex nucleic acid detection methods
US20140242582A1 (en) 2013-02-28 2014-08-28 Ariosa Diagnostics, Inc. Detection of genetic abnormalities using ligation-based detection and digital pcr
US9567632B2 (en) 2013-03-19 2017-02-14 New England Biolabs, Inc. Enrichment of target sequences
KR102160389B1 (ko) 2013-08-05 2020-09-28 트위스트 바이오사이언스 코포레이션 드 노보 합성된 유전자 라이브러리
CN105659091A (zh) 2013-08-19 2016-06-08 卓异生物公司 用于单分子检测的测定及其应用
EP3175236B1 (en) 2014-08-01 2019-08-21 Ariosa Diagnostics, Inc. Detection of target nucleic acids using hybridization
US20180023138A1 (en) 2014-11-01 2018-01-25 Singular Bio, Inc. Assays for Single Molecule Detection and Use Thereof
WO2016134191A1 (en) 2015-02-18 2016-08-25 Singular Bio, Inc. Assays for single molecule detection and use thereof

Similar Documents

Publication Publication Date Title
AU2024216525B2 (en) Methods for spatial analysis using rna-templated ligation
US11332778B2 (en) Methods and systems for nucleic acid amplification
US9255291B2 (en) Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
CN103781918B (zh) 用于组织样本中核酸的局部或空间检测的方法和产品
CN104619894B (zh) 用于非期望核酸序列的阴性选择的组合物和方法
US12516446B2 (en) Array and method for detecting spatial information of nucleic acids
JP2021522796A5 (enExample)
US10233443B2 (en) Reducing adapter dimer formation
US20150050657A1 (en) Targeted enrichment and amplification of nucleic acids on a support
JP2023513955A5 (enExample)
JP2020530779A5 (enExample)
JP2020517298A5 (enExample)
WO2018091676A1 (en) Method for spatial tagging and analysing nucleic acids in a biological specimen
JP2010537643A (ja) 代替的な核酸配列決定法
CZ20031582A3 (cs) Izotermická amplifikace nukleových kyselin na pevném povrchu
JP6716465B2 (ja) 集積した単一細胞の配列決定
JP2013509871A5 (enExample)
JP2023539360A5 (enExample)
US10392652B2 (en) Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end
WO2019014218A2 (en) SEQUENCING METHOD FOR DETECTION OF GENOMIC REARRANGEMENTS
JP2026040472A5 (enExample)
CN104093854A (zh) 表征组合物中的rna的方法和试剂盒
JP2024003255A5 (enExample)
RU2011141720A (ru) Молекулярное обнаружение, основанное на абскрипции
JP2022515774A5 (enExample)